This work presents a promising clinical molecular diagnostics for early stage lung cancer. This novel diagnostic method utilized the loopmediated isothermal amplification （LAMP）, micro fluidic chips and a confocal o...This work presents a promising clinical molecular diagnostics for early stage lung cancer. This novel diagnostic method utilized the loopmediated isothermal amplification （LAMP）, micro fluidic chips and a confocal optical detector with a nonlinear fluorescent filter processor. An isothermal amplification based microfluidic chip for the early diagnostics of lung cancer was developed and a confocal optical detector was improved by a novel real-time fluorescent filter to sensitively monitor the DNA amplification procedure with high signal to noise ratio and fluorescence collecting ability. Experiment showed that a rapid diagnostic of lung cancer by detecting the existence of the CEA mRNA could be performed in only 5 laL of reaction assay in less than 45 min. While the traditional intube RTPCR set consumed more than 25 -μL of the assay and took more than 90 rain.展开更多
基金supported by the National Supporting Plan of China (2011BAZ03288, 2012BAI23B00)the National Program on Key Basic Research Projects of China (2011CB707701)+2 种基金the National Natural Science Foundation of China (60978064)the Science Plan Foundation of Zhejiang (2012C31G3210006)the TSINGHUA-SIEMENS Collective Foundation (20103000273)
文摘This work presents a promising clinical molecular diagnostics for early stage lung cancer. This novel diagnostic method utilized the loopmediated isothermal amplification （LAMP）, micro fluidic chips and a confocal optical detector with a nonlinear fluorescent filter processor. An isothermal amplification based microfluidic chip for the early diagnostics of lung cancer was developed and a confocal optical detector was improved by a novel real-time fluorescent filter to sensitively monitor the DNA amplification procedure with high signal to noise ratio and fluorescence collecting ability. Experiment showed that a rapid diagnostic of lung cancer by detecting the existence of the CEA mRNA could be performed in only 5 laL of reaction assay in less than 45 min. While the traditional intube RTPCR set consumed more than 25 -μL of the assay and took more than 90 rain.
