Gold nanoparticles recently showed great interest for many uses including food, drug and medical applications. The algae </span><i style="font-family:"white-space:normal;">Undaria</i...Gold nanoparticles recently showed great interest for many uses including food, drug and medical applications. The algae </span><i style="font-family:"white-space:normal;">Undaria</i><span style="font-family:"white-space:normal;"> <i>sp.</i></span><span style="font-family:"white-space:normal;"> well known as wakame in South Asia are considered to be large edible brown algae. It provides nutritious source of dietary fiber, vitamin Bs and mineral. The present study aimed to investigate the use of </span><i style="font-family:"white-space:normal;">Undaria</i><span style="font-family:"white-space:normal;"> <i>sp.</i></span><span style="font-family:"white-space:normal;"> for green synthesis of metallic gold nanoparticles. The synthesized nanoparticles were characterized for physicochemical properties including size measurement and tested </span><i style="font-family:"white-space:normal;">in</i><span style="font-family:"white-space:normal;"> <i>vitro</i></span><span style="font-family:"white-space:normal;"> for their effect on viability of human non-small lung cancer H-460 cell line using the MTT assay. From the results, brown algae were able to chemically form nanoparticles with chloroauric acid solution possibly due to the sulphated polysaccharides found in algae. The particle sizes were found to be approximately 10 nm. The gold nanoparticles stabilized by the algae could decrease the cancer cell viability. However, the properties and biological activity of nanoparticles seemed to depend upon reaction time and temperature. Conclusively, gold nanoparticles synthesized and stabilized by the algae could decrease the cancer cell viability, thus indicating the potential of such nanoparticles for further study for anticancer activity.展开更多
Objectives: We retrospectively analyzed whether the severity of Chronic Obstructive Pulmonary Disease (COPD) affected disease-specific survival in Non-Small-Cell Lung Cancer (NSCLC) patients after surgical resection. ...Objectives: We retrospectively analyzed whether the severity of Chronic Obstructive Pulmonary Disease (COPD) affected disease-specific survival in Non-Small-Cell Lung Cancer (NSCLC) patients after surgical resection. Methods: We enrolled 210 NSCLC patients who underwent curative surgery between 2009 and 2011. Classification of COPD severity was based on guidelines of the Global Initiative for Chronic Obstructive Lung Disease (GOLD). Results: A total of 55 patients were diagnosed with COPD. The 5-year disease-specific survival of patients with COPD was not different compared with that of patients without COPD. Among the COPD patients, 40 were classified as GOLD 1, 13 as GOLD 2, and 2 as GOLD 3. Although the number of patients with GOLD 2 - 3 was small, the 5-year disease-specific survival of patients with GOLD 2 - 3 was significantly poorer. We found the prognostic significance of GOLD 2 - 3 in univariate analysis, but failed to find this in multivariate analysis. Conclusions: There is a possibility that the severity of COPD might be useful to predict the prognosis of NSCLC patients. Further studies with large study population are needed.展开更多
This paper investigated the numerical scheme extended to solve the hyperbolic non-Fourier form of bioheat transfer equation and the experimental trials were conducted to validate the numerical simulation. MNPs were pr...This paper investigated the numerical scheme extended to solve the hyperbolic non-Fourier form of bioheat transfer equation and the experimental trials were conducted to validate the numerical simulation. MNPs were prepared via co-precipitation and modified with a silica layer. The amino modified Fe3O4/SiO2 nanoshells were covered with gold colloids producing nanoshells of Fe3O4/SiO2/Au (MNSs). In vitro assays were performed to determine the effect of apoptosis of QU-DB lung cancer cells based on the cells morphology changes. Cell damage was reduced by decreasing the power density of laser. Also, a larger area of damage on cell culture plates was observed at longer intervals of laser irradiation. The effect of nanoshell concentration and irradiation rate has been evaluated. A maximum temperature rise of 6°C was achieved at 184 W/cm2 and concentration of 0.01 mg/ml. The experiment confirmed a hyperbolic behaviour of thermal propagation. The results revealed that the three-dimensional implementation of bioheat equation is likely to be more accurate than the two-dimensional study.展开更多
文摘Gold nanoparticles recently showed great interest for many uses including food, drug and medical applications. The algae </span><i style="font-family:"white-space:normal;">Undaria</i><span style="font-family:"white-space:normal;"> <i>sp.</i></span><span style="font-family:"white-space:normal;"> well known as wakame in South Asia are considered to be large edible brown algae. It provides nutritious source of dietary fiber, vitamin Bs and mineral. The present study aimed to investigate the use of </span><i style="font-family:"white-space:normal;">Undaria</i><span style="font-family:"white-space:normal;"> <i>sp.</i></span><span style="font-family:"white-space:normal;"> for green synthesis of metallic gold nanoparticles. The synthesized nanoparticles were characterized for physicochemical properties including size measurement and tested </span><i style="font-family:"white-space:normal;">in</i><span style="font-family:"white-space:normal;"> <i>vitro</i></span><span style="font-family:"white-space:normal;"> for their effect on viability of human non-small lung cancer H-460 cell line using the MTT assay. From the results, brown algae were able to chemically form nanoparticles with chloroauric acid solution possibly due to the sulphated polysaccharides found in algae. The particle sizes were found to be approximately 10 nm. The gold nanoparticles stabilized by the algae could decrease the cancer cell viability. However, the properties and biological activity of nanoparticles seemed to depend upon reaction time and temperature. Conclusively, gold nanoparticles synthesized and stabilized by the algae could decrease the cancer cell viability, thus indicating the potential of such nanoparticles for further study for anticancer activity.
文摘Objectives: We retrospectively analyzed whether the severity of Chronic Obstructive Pulmonary Disease (COPD) affected disease-specific survival in Non-Small-Cell Lung Cancer (NSCLC) patients after surgical resection. Methods: We enrolled 210 NSCLC patients who underwent curative surgery between 2009 and 2011. Classification of COPD severity was based on guidelines of the Global Initiative for Chronic Obstructive Lung Disease (GOLD). Results: A total of 55 patients were diagnosed with COPD. The 5-year disease-specific survival of patients with COPD was not different compared with that of patients without COPD. Among the COPD patients, 40 were classified as GOLD 1, 13 as GOLD 2, and 2 as GOLD 3. Although the number of patients with GOLD 2 - 3 was small, the 5-year disease-specific survival of patients with GOLD 2 - 3 was significantly poorer. We found the prognostic significance of GOLD 2 - 3 in univariate analysis, but failed to find this in multivariate analysis. Conclusions: There is a possibility that the severity of COPD might be useful to predict the prognosis of NSCLC patients. Further studies with large study population are needed.
文摘This paper investigated the numerical scheme extended to solve the hyperbolic non-Fourier form of bioheat transfer equation and the experimental trials were conducted to validate the numerical simulation. MNPs were prepared via co-precipitation and modified with a silica layer. The amino modified Fe3O4/SiO2 nanoshells were covered with gold colloids producing nanoshells of Fe3O4/SiO2/Au (MNSs). In vitro assays were performed to determine the effect of apoptosis of QU-DB lung cancer cells based on the cells morphology changes. Cell damage was reduced by decreasing the power density of laser. Also, a larger area of damage on cell culture plates was observed at longer intervals of laser irradiation. The effect of nanoshell concentration and irradiation rate has been evaluated. A maximum temperature rise of 6°C was achieved at 184 W/cm2 and concentration of 0.01 mg/ml. The experiment confirmed a hyperbolic behaviour of thermal propagation. The results revealed that the three-dimensional implementation of bioheat equation is likely to be more accurate than the two-dimensional study.
