Extraction is most commonly used sample preparation technique in quantitative determination of all-trans-lutein in spinach. In our study RP-HPLC was applied to identify the constituents of spinach extract and to quant...Extraction is most commonly used sample preparation technique in quantitative determination of all-trans-lutein in spinach. In our study RP-HPLC was applied to identify the constituents of spinach extract and to quantify all-trans-lutein content with the mobile phase consisted of acetone and water. Due to sensitivity of all-trans-lutein, we compared effects of different extraction conditions;air and nitrogen atmosphere and ethanol, ethanol with added 0.1% TBHQ and ethanol with added 0.1% BHT as solvents. Results for the all-trans-lutein content in the spinach samples and spinach samples with standard addition obtained in air and in nitrogen confirmed the induced degradation of all-trans-lutein in oxygen from the air. Ethanol containing synthetic antioxidant TBHQ under nitrogen atmosphere gave the highest yield of extraction and recovery of all-trans-lutein from spinach.展开更多
[ Objective] This paper was to evaluate the methods of breaking stiffness cell wall of Chlorella sp. and extracting and testing functional antioxidant by ethanol and DPPH method separately. [Method] Extractions were p...[ Objective] This paper was to evaluate the methods of breaking stiffness cell wall of Chlorella sp. and extracting and testing functional antioxidant by ethanol and DPPH method separately. [Method] Extractions were performed at different extraction times （60, 120 and 180 min） at 37 ℃. And the radical scavenging activity of Chlorella spo extract was assayed by the DPPH （ 1,1-Diphenyl-2- picrylhydrazy[） method. [ Result ] The optimal conditions for ethanol extraction from Chlorella sp. were ethanol concentration of 90%, substrate consistency of 5% （w/v） and treating time of 180 min, under which, a concentration of 135.5 mg/L was obtained. Antioxidant pigments obtained from alcoholic extraction from Chlorella sp. showed high free radical scavenging ability. The efficiency increased with increasing concentration of solid microalgae powder, which could reach to 88%. [ Conclusion] Ethanol extraction method is simple and feasible. However, the efficiency of extraction is not high enough, which will limit the yield of antioxidant production from economic prospective.展开更多
文摘Extraction is most commonly used sample preparation technique in quantitative determination of all-trans-lutein in spinach. In our study RP-HPLC was applied to identify the constituents of spinach extract and to quantify all-trans-lutein content with the mobile phase consisted of acetone and water. Due to sensitivity of all-trans-lutein, we compared effects of different extraction conditions;air and nitrogen atmosphere and ethanol, ethanol with added 0.1% TBHQ and ethanol with added 0.1% BHT as solvents. Results for the all-trans-lutein content in the spinach samples and spinach samples with standard addition obtained in air and in nitrogen confirmed the induced degradation of all-trans-lutein in oxygen from the air. Ethanol containing synthetic antioxidant TBHQ under nitrogen atmosphere gave the highest yield of extraction and recovery of all-trans-lutein from spinach.
文摘[ Objective] This paper was to evaluate the methods of breaking stiffness cell wall of Chlorella sp. and extracting and testing functional antioxidant by ethanol and DPPH method separately. [Method] Extractions were performed at different extraction times （60, 120 and 180 min） at 37 ℃. And the radical scavenging activity of Chlorella spo extract was assayed by the DPPH （ 1,1-Diphenyl-2- picrylhydrazy[） method. [ Result ] The optimal conditions for ethanol extraction from Chlorella sp. were ethanol concentration of 90%, substrate consistency of 5% （w/v） and treating time of 180 min, under which, a concentration of 135.5 mg/L was obtained. Antioxidant pigments obtained from alcoholic extraction from Chlorella sp. showed high free radical scavenging ability. The efficiency increased with increasing concentration of solid microalgae powder, which could reach to 88%. [ Conclusion] Ethanol extraction method is simple and feasible. However, the efficiency of extraction is not high enough, which will limit the yield of antioxidant production from economic prospective.
