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N-acetylcysteine and zinc sulphate abate di-2-ethylhexyl phthalate-mediated reproductive dysfunction in rats:Focus on oxidative and sex hormone receptors mechanisms
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作者 Victor Oghenekparobo Emojevwe Mega Obukohwo Oyovwi +7 位作者 Kayode Ezekiel Adewole Peggy Ejiro Ohwin Adeniran Oluwadamilare Akinola Alexander Obidike Naiho Eze Kingsley Nwangwa Victor Omo-Idonije Motunrayo Lade-Ige Benneth Ben-Azu 《Asian pacific Journal of Reproduction》 CAS 2024年第5期228-240,共13页
Objective:To investigate the potential of N-acetylcysteine(NAC)and zinc sulphate(ZnSO_(4))in mitigating reproductive dysfunction caused by di-2-ethylhexyl phthalate(DEHP)in rats and to understand the underlying mechan... Objective:To investigate the potential of N-acetylcysteine(NAC)and zinc sulphate(ZnSO_(4))in mitigating reproductive dysfunction caused by di-2-ethylhexyl phthalate(DEHP)in rats and to understand the underlying mechanisms,specifically oxidative stress and sex hormone receptor activity.Methods:Thirty-five male Wistar rats were randomly divided into five equal groups(n=7 per group).Group 1 was administered 0.5 mL of distilled water and served as the control group.Group 2 was given only DEHP(750 mg/kg/day),while group 3,4 and 5 were given DEHP(750 mg/kg/day)plus NAC(100 mg/kg/day),DEHP(750 mg/kg/day)plus ZnSO_(4)(0.5 mg/kg/day),and DEHP(750 mg/kg/day)plus NAC(100 mg/kg/day)as well as ZnSO_(4)(0.5 mg/kg/day),respectively.All treatments lasted for 21 days.Samples were obtained after the rats were sacrificed,and hormones levels in the serum and markers of oxidative stress in the testicles were analyzed using the enzyme-linked immunosorbent assay.The amount of androgen receptors in the testicles was determined by immunohistochemistry,and the susceptibility of testosterone and DEHP to bind to androgen receptor and 5α-reductase was determined by molecular docking studies.Results:DEHP decreased reproductive hormones,testicular antioxidant enzymes,increased malondialdehyde levels,and negatively impacted histology of the pituitary and testes.NAC or ZnSO_(4) treatment showed a marked improvement in testicular antioxidant status and hormone levels,as well as a positive effect on the histology of the pituitary and testes.The combination of both treatments appeared to be more effective.The affinity of DEHP to bind to androgen receptors may lead to disruption of androgen receptor signaling,which can further result in dysfunction of hormones related to androgen.However,NAC is more likely to form stronger binding interactions with follicle stimulating hormone and luteinizing hormone receptors,as well as gonadotropin-releasing hormone receptors,when compared to DEHP.Conclusions:The possibility that NAC and ZnSO_(4) could downregulate DEHP-induced sex hormone changes is suggested by their potential to reduce toxicity. 展开更多
关键词 Di-2-ethylhexyl phthalate TESTOSTERONE Androgen receptor 5Α-REDUCTASE OESTROGEN luteinizing hormone
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Influence of leptin on luteinizing hormone and follicle stimulating hormone secreted from cultured rat anterior pituitary cells 被引量:2
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作者 Yuebing Qiao Xiuyan Ma Huixian Cui 《Neural Regeneration Research》 SCIE CAS CSCD 2008年第6期656-658,共3页
BACKGROUND: Leptin may regulate reproductive function via release of hypothalamic neuropeptide Y. However, it is unknown whether this regulatory effect is limited to the hypothalamus. OBJECTIVE: To detect the effect... BACKGROUND: Leptin may regulate reproductive function via release of hypothalamic neuropeptide Y. However, it is unknown whether this regulatory effect is limited to the hypothalamus. OBJECTIVE: To detect the effect of different dosages of leptin on luteinizing hormone (LH) and follicle stimulating hormone (FSH) secretion from in vitro cultured rat anterior pituitary cells. DESIGN: Contrast study based on cells. SETTING: This study was performed in the Basic Institute of Chengde Medical College, Chengde City, Hebei Province, China from March to June 2007. MATERIALS: Eighteen female Wistar rats of three months of age, weighing 200-220 g, and of clean grade were used. Leptin was provided by Peprotech Company, DMEM culture medium by Invitrogen Company, and the radioimmunological kit by Beijing Zhongshan Jinqiao Biotechnology Co., Ltd. METHODS: Three glandular organs were regarded as one group for culture of anterior pituitary cells. In the control group, saline was added to the culture medium instead of leptin. In the leptin group, leptin was prepared into different concentrations of 1×10^-12, 1×10^-11, 1×10^-9, 1×10^-7, and 1×10^-6 mol/L for stimulation of cultured cells. The culture supernatant was obtained at three hours after additional of saline/leptin. MAIN OUTCOME MEASURES: Contents of LH and FSH were detected by radioimmunology. RESULTS: Following leptin stimulation, LH release increased with increasing concentrations of leptin up to 1×10^-9 mol/L, where LH release peaked. LH release then progressively decreased with increasing leptin concentrations (P 〈 0.01). LH release in the leptin (1×10^-12, 1×10^-11, 1×10^-7, and 1×10^-6 mol/L) groups was significantly higher than that in the control group (P 〈 0.01). FSH content in the leptin (1×10^-11, 1×10^-9, and 1×10^-7 mol/L) groups was significantly higher than that in the control group (P 〈 0.01). CONCLUSION: Leptin can directly affect pituitary tissue to promote the secretion of LH and FSH in a dose-dependent manner. 展开更多
关键词 LEPTIN anterior pituitary luteinizing hormone follicle stimulating hormone
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Combination immunotherapy with Survivin and luteinizing hormone-releasing hormone fusion protein in murine breast cancer model 被引量:2
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作者 Himani Garg Rohit Singh Hada +2 位作者 Jagdish C Gupta G P Talwar Shweta Dubey 《World Journal of Clinical Oncology》 CAS 2018年第8期188-199,共12页
AIM To investigate the therapeutic potential of two recombinant proteins, Survivin and luteinizing hormone-releasing hormone (LHRH) fusion protein [LHRH(6 leu)-LTB] for immunotherapy of breast cancer.METHODS Murine 4 ... AIM To investigate the therapeutic potential of two recombinant proteins, Survivin and luteinizing hormone-releasing hormone (LHRH) fusion protein [LHRH(6 leu)-LTB] for immunotherapy of breast cancer.METHODS Murine 4 T-1 breast cancer model was used to evaluate the efficacy of recombinant proteins in vivo. Twenty four Balb/c mice were divided into 4 groups of 6 mice each. Recombinant Survivin and LHRH fusion protein, alone or in combination, were administered along with immunomodulator Mycobacterium indicus pranii (MIP) in Balb/c mice. Unimmunized or control group mice were administered with phosphate buffer saline. Each group was then challenged with syngeneic 4 T-1 cells to induce the growth of breast tumor. Tumor growth was monitored to evaluate the efficacy of immune-response in preventing the growth of cancer cells.RESULTS Preventive immunization with 20 μg recombinant Survivin and MIP was effective in suppressing growth of 4 T-1 mouse model of breast cancer (P = 0.04) but 50 μg dose was ineffective in suppressing tumor growth. However, combination of Survivin and LHRH fusion protein was more effective in suppressing tumor growth (P = 0.02) as well as metastasis in vivo in comparison to LHRH fusion protein as vaccine antigen alone.CONCLUSION Recombinant Survivin and MIP suppress tumor growth significantly. Combining LHRH fusion protein with Survivin and MIP enhances tumor suppressive effects marginally which provides evidence for recombinant Survivin and LHRH fusion protein as candidates for translating the combination cancer immunotherapy approaches. 展开更多
关键词 IMMUNOTHERAPY SURVIVIN luteinizing hormone-releasing hormone fusion protein COMBINATION IMMUNOTHERAPY Breast cancer
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Long-term effectiveness of luteinizing hormone-releasing hormone agonist or antiandrogen monotherapy in elderly men with localizect prostate cancer (T1-2) : a retrospective study 被引量:1
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作者 Rupesh Raina Geetu Pahalajani +1 位作者 Ashok Agarwal Craig Zippe 《Asian Journal of Andrology》 SCIE CAS CSCD 2007年第2期253-258,共6页
Aim: To evaluate the long-term effectiveness, side effects and compliance rates of two types of drugs (luteinizing hormone-releasing hormone [LHRH] agonist and antiandrogen) that were used individually to treat pat... Aim: To evaluate the long-term effectiveness, side effects and compliance rates of two types of drugs (luteinizing hormone-releasing hormone [LHRH] agonist and antiandrogen) that were used individually to treat patients with localized prostate cancer (T1-2) at our institution. Methods: Ninety-seven patients who were diagnosed in the period from April 1997 to January 2000 as having clinically localized prostate cancer (T1-2) received either LHRH agonist (leuprolide acetate 7.5 mg/month) monotherapy (group 1, n = 62) or antiandrogen monotherapy (group 2, n = 35; 18 received bicalutamide 50 mg q.d., 13 received nilutamide 150 mg t.i.d, and 4 received flutamide 250 mg t.i.d.). The mean age in both groups was 76 years. Results: The mean follow-up time was (50.8 ±8.5) months in group 1 and (43.1 ± 2.2) months in group 2. Prostate-specific antigen (PSA) levels rose in only 1 of the 62 patients (1.6%) in group 1, and in 20 of the 35 patients (57.1%) in group 2. In group 2, 10 of the 20 patients (50 %) with increasing PSA levels were treated with LHRH salvage therapy, and eight (80%) responded. Hot flashes (54.8%) and lethargy (41.9%) were the most common side effects in group 1. In contrast, nipple-tenderness (40%) and light-dark adaptation (17.1%) were more often seen in group 2. Only 1 of the 62 patients (1.6%) in group 1 switched to another medication because of adverse side effects; whereas 8 of the 35 patients (22.9%) in group 2 did so. Conclusion: Unlike antiandrogen monotherapy, LHRH agonist monotherapy provided long-term durable control of localized prostate cancer (T1-2). It can also be an effective treatment option for patients whose disease failed to respond to antiandrogen monotherapy. The limitations of our study are the lack of health outcomes analysis and a small sample size. 展开更多
关键词 localized prostate cancer ANTIANDROGEN prostate-specific antigen luteinizing hormone-releasing hormone agonist ANDROGEN ablation MONOTHERAPY
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Effect of Luteinizing Hormone-Releasing Hormone Analogue on the Sexual Behavior of Sacalia quadriocellata 被引量:1
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作者 HE Bin LIU Yuxiang +2 位作者 SHI Haitao FU Lirong WANG Jichao 《Asian Herpetological Research》 SCIE 2010年第1期40-43,共4页
Luteinizing hormone-releasing hormone(LHRH) is known to influence sexual behavior in many vertebrate taxa, but there have been no systematic studies on the role of LHRH in sexual behavior of turtles. We tested the hyp... Luteinizing hormone-releasing hormone(LHRH) is known to influence sexual behavior in many vertebrate taxa, but there have been no systematic studies on the role of LHRH in sexual behavior of turtles. We tested the hypotheses that exogenous LHRH analogues would induce sexual behavior of male Four-eyed turtle, Sacalia quadriocellata. We examined this by challenging males with intramuscular injections of mammalian luteinizing hormone-releasing hormone analogue(LHRH-A), human chorionic gonadotropin(HCG), or a combination of the two, and subsequently exposing them to sexually receptive females for behavioral observation. Our data show that the injection of only HCG could not, while that of only LHRH-A could, facilitate sexual behavior along with testicular recrudescence and spermatogenesis in S. quadriocellata. The injection of both LHRH-A and HCG would induce more drastic sexual behavior of the animals than that of LHRH-A alone, indicating HCG enhances the effects of LHRH-A induced sexual behavior. However, different pharmacological dosages of LHRH-A(0.5 μg, 1 μg, 2 μg per 100 g bodyweight) did not correspond to different activity levels. Though the mechanism of LHRH effect was not determined, this study may support that the sexual behavior of S. quadriocellata which occurs at the beginning of the injection despite regression of the gonads. This is the first report on the exogenous LHRH-A induced sexual behavior for this species. 展开更多
关键词 Four-eyed turtle Sacalia quadriocellata luteinizing hormone-releasing hormone sexual behavior
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Effects of Estrogen and/or Progesterone on Morphology of Pituitary Luteinizing Hormone Cells 被引量:1
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作者 周寿康 任惠民 +3 位作者 王健 赵伟 顾敦瑜 谢衷明 《Journal of Reproduction and Contraception》 CAS 2001年第1期3-14,共12页
Objective To identify the morphological characteristics of pituitary luteinizing hormone (LH) cells after exogenous gonadal hormone treatment Methods Effects of various doses of estrogen, progesterone and their... Objective To identify the morphological characteristics of pituitary luteinizing hormone (LH) cells after exogenous gonadal hormone treatment Methods Effects of various doses of estrogen, progesterone and their combination on morphological parameters, including the size and shape of pituitary LH cells, the size of endocellular vacuoles, were observed and measured by immuno histochemistry and computer image analysis. Results Different kinds of gonadal hormones could recover the magnified LH cells to the normal level in ovariectomized rats. However, their final effects on the gonadotrophin levels and the cellular morphological characters of the LH cells were different. The low dose of estrogen elicited abundant hormone stored in the LH cells to an easy released status with a lot of different size of vacuoles. On the contrary, the high dose of estrogen inhibited the storage of LH, and the LH cells were filled with secretory granules and few vacuoles. The progesterone could promote the storage of LH in an uneasy released status. The administration of estrogen progesterone combination not only inhibited the storage of LH, but also the release of LH. In this group, the LH cells containing a large amount of secretory granules and a few vacuoles showed a better uniform shape compared with those administrated with high dose of estrogen. Conclusion: Different kinds of gonadal hormones could reverse the excessive secretion of LH and recover the morphological change of LH cells to the normally physiological condition. 展开更多
关键词 lh secretion VACUOLE secretory granules gonadal hormone
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Beneficial effects of a decreased meal frequency on nutrient utilization,secretion of luteinizing hormones and ovarian follicular development in gilts 被引量:1
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作者 Lun Hua Lianpeng Zhao +10 位作者 Zhengyu Mao Wentao Li Jing Li Xuemei Jiang Lianqiang Che Shengyu Xu Yan Lin Zhengfeng Fang Bin Feng De Wu Yong Zhuo 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2021年第3期1098-1111,共14页
Background:Replacement gilts are typically fed ad libitum,whereas emerging evidence from human and rodent studies has revealed that time-restricted access to food has health benefits.The objective of this study was to... Background:Replacement gilts are typically fed ad libitum,whereas emerging evidence from human and rodent studies has revealed that time-restricted access to food has health benefits.The objective of this study was to investigate the effect of meal frequency on the metabolic status and ovarian follicular development in gilts.Methods:A total of 36 gilts(Landrace×Yorkshire)with an age of 150±3 d and a body weight of 77.6±3.8 kg were randomly allocated into one of three groups(n=12 in each group),and based on the group allocation,the gilts were fed at a frequency of one meal(T1),two meals(T2),or six meals per day(T6)for 14 consecutive weeks.The effects of the meal frequency on growth preference,nutrient utilization,short-chain fatty acid production by gut microbial,the post-meal dynamics in the metabolic status,reproductive hormone secretions,and ovarian follicular development in the gilts were measured.Results:The gilts in the T1 group presented a higher average daily gain(+48 g/d,P<0.05)and a higher body weight(+4.9 kg,P<0.05)than those in the T6 group.The meal frequency had no effect on the apparent digestibility of dry matter,crude protein,ether extract,ash,and gross energy,with the exception that the T1 gilts exhibited a greater NDF digestibility than the T6 gilts(P<0.05).The nitrogen balance analysis revealed that the T1 gilts presented decreased urine excretion of nitrogen(−8.17 g/d,P<0.05)and higher nitrogen retention(+9.81 g/d,P<0.05),and thus exhibited higher nitrogen utilization than the T6 gilts.The time-course dynamics of glucose,α-amino nitrogen,urea,lactate,and insulin levels in serum revealed that the T1 group exhibited higher utilization of nutrients after a meal than the T2 or T6 gilts.The T1 gilts also had a higher acetate content and SCFAs in feces than the T6 gilts(P<0.05).The age,body weight and backfat thickness of the gilts at first estrous expression were not affected by the meal frequency,but the gilts in the T1 group had higher levels of serum luteinizing hormone on the 18th day of the 3rd estrus cycle and 17β-estradiol,a larger number of growing follicles and corpora lutea,and higher mRNA expression levels of genes related to follicular development on the 19th day of the 3rd estrus cycle.Conclusions:The current findings revealed the benefits of a lower meal frequency equal feed intake on nutrient utilization and reproductive function in replacement gilts,and thus provide new insights into the nutritional strategy for replacement gilts,and the dietary pattern for other mammals,such as humans. 展开更多
关键词 GILTS luteinizing hormone Meal frequency Nutrient utilization Ovarian follicular development
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Immunohistochemical observation on luteinizing hormone in rat testes before and after testicular capsulotomy
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作者 Da-NianQIN MaryA.Lung 《Asian Journal of Andrology》 SCIE CAS CSCD 2001年第3期227-230,共4页
Aim: In the testicular capsulotomized rats, although there was a significant increase in the luteinizing hormone (LH)levels, the secretion of testosterone remained low. In order to clarify the mechanisms of this pheno... Aim: In the testicular capsulotomized rats, although there was a significant increase in the luteinizing hormone (LH)levels, the secretion of testosterone remained low. In order to clarify the mechanisms of this phenomenon, the bindingof endogenous LH to the testes were observed before and after testicular capsulotomy. Methods; Peroxidase-anti-peroxidase (PAP) method was used to detect the binding of LH to the testes in rats. Results; An intense positivestaining of LH was found in the Leydig cells of both the normal and sham-operated control testes. However, at 40 d af-ter operation, the LH immunoreactivity was decreased in the Leydig cells of the capsulotomized testis. By d 60, onlyvery weak positive staining could be observed in these cells. Conclusion; A progressive reduction of endogenousLH binding to the testis occurred in the capsulotomized rat. (Asian J Androl 2001 Sep; 3 : 227 - 230) 展开更多
关键词 TESTIS testicular capsulotomy luteinizing hormone IMMUNOHISTOCHEMISTRY
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Effect of Kisspeptin on Regulation of Growth Hormone and Luteinizing Hormone in Lactating Dairy Cows
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作者 B. K. Whitlock J. A. Daniel +3 位作者 R. R. Wilborn H. S. Maxwell B. P. Steele J. L. Sartin 《Journal of Animal Science and Biotechnology》 SCIE CAS 2011年第3期131-140,共10页
Kisspeptin (KP) , a neuroendocrine regulator of reproduction,is hypothesized to be an integrator of metabolism and hormones critical to the regulation of reproduction. Lactation is associated with enhanced growth ho... Kisspeptin (KP) , a neuroendocrine regulator of reproduction,is hypothesized to be an integrator of metabolism and hormones critical to the regulation of reproduction. Lactation is associated with enhanced growth hormone (GH) responsiveness and reduced fertility. Our study was designed to determine the effects of lactation on KP-stimulated GH and luteinizing hormone (LH) secretion. Five non-lactating and five lactating dairy cows were used in the study. Experiments were conducted with lactating cows at weeks 1,5 and 11 after parturition. The experimental treatments (saline and KP [100 and 400 pmol / kg body weight]) were given intravenously and blood was collected and plasma was stored until later assay to determine concentrations of GH,LH,progesterone and nonesterified fatty acids. We found that neither dose of KP stimulated an increase in GH secretion. The low dose ofKP increased (P 〈0. 05) LH concentrations only in lactating cows. The higher dose of KP elicited an increase in circulating LH concentrations in both lactating and non-lactating cows. The lower dose of KP increased (P 〈 0. 05) the area under the curve for LH only in cows during week 5 of lactation,and the area under the curve of LH following the highest dose of KP was greater (P 〈 0. 05) in cows during week 5 of lactation than that for the other groups of cows. In summary,lactation status and stage of lactation did not change the sensitivity of the GH system to KP. However,an effect of stage of lactation on KP-stimulated LH secretion was detected in the dairy cows. Study of the KP system during lactation in dairy cows may provide critical insights into the mechanisms for lactation-associated changes in the reproductive axis. 展开更多
关键词 growth hormone KISSPEPTIN LACTATION luteinizing hormone
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Plasma levels of luteinizing hormone and prolactin in relation to double brooding in Great Tit(Parus major)
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作者 Xudong Li Wenyu Xu +2 位作者 Jiangping Yu Wutong Zhang Haitao Wang 《Avian Research》 SCIE CSCD 2022年第1期102-107,共6页
The reproductive behaviors of birds are mainly controlled by the hypothalamus-pituitary-gonad axis.Many studies have shown that reproductive hormones are tightly linked to the breeding sub-stages.However,only a few st... The reproductive behaviors of birds are mainly controlled by the hypothalamus-pituitary-gonad axis.Many studies have shown that reproductive hormones are tightly linked to the breeding sub-stages.However,only a few studies have examined the temporal trend of hormone levels among different reproductive stages in multiple brooded species.We investigated the changes in plasma luteinizing hormone(LH)and prolactin(PRL)concentrations during different reproductive stages of the facultative double-brooded Great Tit(Parus major).We found that the concentrations of LH and PRL in females were significantly higher than those in males.Females had significantly higher LH and lower PRL concentrations in the pre-breeding period than in the first/second brooding periods,and there were no significant changes between the first and second brooding periods.The concentrations of LH and PRL in males had no significant difference between the pre-breeding period and the first brooding periods,while LH and PRL concentrations in the second brooding period were significantly higher than those in the first brooding period.We conclude that there are sex-based differences between LH and PRL at different stages of reproduction.The changes in LH and PRL in both males and females should be related to their physiological functions.Especially for males,individuals with higher levels of LH and PRL are more likely to maintain second clutches. 展开更多
关键词 BIRDS Great Tit luteinizing hormone PROLACTIN Seasonal breeding
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血清HE4、LH、FSH对宫颈癌和宫颈上皮内瘤变的鉴别诊断价值
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作者 王衡 高湛 +1 位作者 王蕊 冉繁华 《实用癌症杂志》 2024年第11期1900-1903,1908,共5页
目的探究血清人附睾蛋白4(HE4)、促黄体生成素(LH)、卵泡刺激素(FSH)对宫颈癌和宫颈上皮内瘤变(CIN)的鉴别诊断价值。方法选择89例接受宫颈癌筛查的宫颈病变患者,经病理学或细胞学明确诊断宫颈癌51例、CIN 38例,检测所有患者血清HE4、LH... 目的探究血清人附睾蛋白4(HE4)、促黄体生成素(LH)、卵泡刺激素(FSH)对宫颈癌和宫颈上皮内瘤变(CIN)的鉴别诊断价值。方法选择89例接受宫颈癌筛查的宫颈病变患者,经病理学或细胞学明确诊断宫颈癌51例、CIN 38例,检测所有患者血清HE4、LH、FSH水平,分析宫颈癌患者与CIN患者血清检测指标水平差异,采用受试者特征工作曲线(ROC曲线)、二元logistics回归分析法评价HE4、LH、FSH在疾病鉴别中的价值。结果宫颈癌患者血清HE4、LH、FSH水平均显著高于CIN患者(P<0.05);宫颈病变患者血清HE4与LH、FSH水平呈正相关(γ=0.357、0.392,P<0.05)。ROC曲线分析显示,血清HE4、LH、FSH诊断宫颈癌的敏感性均较低,三者联合诊断效能较单一指标更高,联合诊断曲线下面积为0.859,诊断敏感度88.24%、特异度71.05%;logistics回归分析显示,HE4>62.84 pmol/ml是宫颈癌发病的影响因素(P<0.05)。结论宫颈癌患者血清HE4、LH、FSH水平异常升高,且指标间呈正相关,三者联合用于鉴别诊断宫颈癌和CIN具有一定临床价值。 展开更多
关键词 宫颈癌 宫颈上皮内瘤变 人附睾蛋白 促黄体生成素 卵泡刺激素 价值
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不同繁殖力新疆山羊发情周期血清FSH和LH浓度变化规律研究
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作者 全凯丽 刘建新 +2 位作者 吕雪峰 邢巍婷 郑文新 《黑龙江动物繁殖》 2024年第5期1-6,共6页
为了探究不同繁殖力母羊发情周期血清中促卵泡激素(FSH)和促黄体激素(LH)分泌变化规律,试验选择新疆山羊高繁殖力母羊17只和低繁殖力母羊14只,采用酶联免疫分析法测定母羊发情周期血清中FSH和LH浓度。结果表明:高、低繁殖力母羊发情周期... 为了探究不同繁殖力母羊发情周期血清中促卵泡激素(FSH)和促黄体激素(LH)分泌变化规律,试验选择新疆山羊高繁殖力母羊17只和低繁殖力母羊14只,采用酶联免疫分析法测定母羊发情周期血清中FSH和LH浓度。结果表明:高、低繁殖力母羊发情周期中FSH和LH浓度均呈脉冲波动式分泌,高繁殖力母羊血清中FSH浓度在发情周期第1,5,13,21天出现4个波峰,LH浓度在发情周期第2,5,11,21天出现4次波峰;低繁殖力母羊血清中FSH浓度在发情周期第1,5,21天出现3个波峰,LH浓度在发情周期第5,13,21天出现3次波峰。在发情期和发情前期母羊血清中FSH和LH浓度均高于发情后期和间情期,高、低繁殖力母羊血清中FSH和LH浓度均在发情前期(>17~21 d)开始升高,在发情期(0~48 h)当天达到峰值之后呈波动式下降趋势,且高繁殖力母羊发情周期4个阶段血清中FSH和LH浓度均高于低繁殖力母羊。高、低繁殖力母羊血清中FSH和LH基础浓度均在发情期最高,间情期最低。说明不同繁殖力新疆山羊母羊发情周期中FSH和LH分泌变化规律一致,高繁殖力母羊在整个发情周期中FSH和LH浓度高于低繁殖力母羊且波峰数量多于低繁殖力母羊。 展开更多
关键词 新疆山羊 生殖激素 促卵泡激素(FSH) 促黄体激素(lh) 繁殖力
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重组人生长激素辅助治疗对高龄不孕IVF⁃ET患者血清FSH、E_(2)及LH水平的影响
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作者 张皙卉 卢静 和伟 《分子诊断与治疗杂志》 2024年第8期1437-1440,共4页
目的探讨在实施体外受精⁃胚胎移植(IVF⁃ET)治疗过程中使用重组人生长激素(rhGH)辅助治疗对高龄不孕症女性患者血清卵泡刺激素(FSH)、雌二醇(E_(2))及促黄体生成素(LH)水平的影响。方法回顾性选取2021年1月至2023年8月邯郸市中心医院收... 目的探讨在实施体外受精⁃胚胎移植(IVF⁃ET)治疗过程中使用重组人生长激素(rhGH)辅助治疗对高龄不孕症女性患者血清卵泡刺激素(FSH)、雌二醇(E_(2))及促黄体生成素(LH)水平的影响。方法回顾性选取2021年1月至2023年8月邯郸市中心医院收治的高龄不孕女性102例,依据治疗方案分为对照组[予促性腺激素释放激素(GnRH)拮抗剂方案治疗,n=50]、试验组(予GnRH拮抗剂方案+rhGH辅助治疗方案,n=52);比较两组促排卵结果、性激素水平(FSH、E_(2)、LH)、妊娠结局及不良反应情况。结果试验组Gn总用量、Gn使用天数比对照组少,差异有统计学意义(P<0.05);试验组获卵总个数、可移植胚胎数及优质胚胎数比对照组高,但差异无统计学意义(P>0.05)。两组治疗后的FSH、E_(2)、LH水平均下降,且试验组治疗后的FSH、E_(2)、LH水平均低于对照组,差异有统计学意义(P<0.05)。试验组治疗后取消周期率、流产率低于对照组,临床妊娠率、活产率高于对照组,但差异无统计学意义(P>0.05)。试验组、对照组不良反应发生率比较差异无统计学意义(P>0.05)。结论相较于GnRH拮抗剂方案单独治疗,GnRH拮抗剂+rhGH辅助治疗方案可进一步调节高龄不孕女性FSH、LH、E_(2)水平,提高获卵数、优胚率及临床妊娠率。 展开更多
关键词 重组人生长激素 不孕 IVF⁃ET FSH E_(2) lh
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血清LH、IGF-ⅠSDS、IGF-bp3诊断女童快速进展型中枢性性早熟的价值研究
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作者 邱世超 高婷 +2 位作者 高飞飞 赵婷 刘超 《中国妇幼健康研究》 2024年第11期7-12,共6页
目的分析血清黄体生成素(LH)、胰岛素样生长因子Ⅰ标准差积分(IGF-ⅠSDS)及胰岛素样生长因子结合蛋白3(IGF-bp3)在早期识别快速进展型中枢性性早熟(RP-CPP)和缓慢进展型中枢性性早熟(SP-CPP)患儿中的有效性,为临床进一步提高对女童RP-CP... 目的分析血清黄体生成素(LH)、胰岛素样生长因子Ⅰ标准差积分(IGF-ⅠSDS)及胰岛素样生长因子结合蛋白3(IGF-bp3)在早期识别快速进展型中枢性性早熟(RP-CPP)和缓慢进展型中枢性性早熟(SP-CPP)患儿中的有效性,为临床进一步提高对女童RP-CPP的诊疗水平提供参考资料。方法回顾性分析2021年4月至2023年6月西安交通大学附属儿童医院收治的120例中枢性性早熟(CPP)女童患儿的临床资料,将其纳入研究组,按照线性生长或骨龄成熟加速等条件,将其分为RP-CPP组(71例),SP-CPP组(49例);纳入同期在院体检的青春期发育正常女童64例为对照组。比较不同人群LH、IGF-ⅠSDS及IGF-bp3水平的变化情况,采用多元Logistic回归分析影响儿童RP-CPP的相关危险因素,采用受试者工作特征(ROC)曲线评估LH、IGF-ⅠSDS及IGF-bp3预测RP-CPP的效能。结果研究组的LH、IGF-ⅠSDS、IGF-bp3及骨龄均明显高于对照组,经比较差异均有统计学意义(t值分别为26.256、47.930、20.390、14.008,P<0.05)。LH、IGF-ⅠSDS、IGF-bp3与骨龄均呈正相关(r值分别为0.435、0.502、0.397,P<0.05)。RP-CPP组的LH、IGF-ⅠSDS水平均明显高于SP-CPP组,经比较差异均有统计学意义(t值分别为7.867、33.943,P<0.05),但RP-CPP组与SP-CPP组的IGF-bp3水平比较,差异无统计学意义(P>0.05)。经多元Logistic回归分析显示,LH[OR(95%CI):1.962(1.172~3.285)]、IGF-ⅠSDS[OR(95%CI):1.707(1.142~2.552)]及IGF-bp3[OR(95%CI):2.314(1.221~4.384)]均是影响RP-CPP发生的危险因素(P<0.01)。经ROC曲线分析显示,LH、IGF-ⅠSDS及IGF-bp3单独预测RP-CPP的曲线下面积(AUC)从高到低依次为0.799、0.762、0.714,三者联合预测RP-CPP的AUC为0.864,其明显高于各指标单独预测的结果(P<0.001)。结论CPP女童的血清LH、IGF-ⅠSDS及IGF-bp3水平均存在异常,通过检测患儿的上述指标变化,可有效地预测病情的进展,LH、IGF-ⅠSDS及IGF-bp3水平较高者发生RP-CPP的风险相对较大。 展开更多
关键词 女童 中枢性性早熟 快速进展型中枢性性早熟 血清黄体生成素 胰岛素样生长因子Ⅰ标准差积分 胰岛素样生长因子结合蛋白3
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Association between luteinizing hormone level decrease on the triggering day and pregnancy outcome for in vitro fertilization/intracytoplasmic sperm injection patients following gonadotropin-releasing hormone antagonist protocol: a retrospective cohor
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作者 Qing-Fang Li Min-Yue Tang +3 位作者 Gui-Quan Wang Xi-Jing Chen Guo-Fang Feng Yi-Min Zhu 《Reproductive and Developmental Medicine》 CAS CSCD 2024年第1期8-17,共10页
Objective:Optimal luteinizing hormone(LH)levels forin vitro fertilization/intracytoplasmic sperm injection(IVF/ICSI)patients following the gonadotropin-releasing hormone(GnRH)antagonist protocol remain controversial.T... Objective:Optimal luteinizing hormone(LH)levels forin vitro fertilization/intracytoplasmic sperm injection(IVF/ICSI)patients following the gonadotropin-releasing hormone(GnRH)antagonist protocol remain controversial.This study aimed to estimate the association between serum LH levels on the triggering day(LHLOT)and clinical pregnancy outcomes in IVF/ICSI patients following the GnRH antagonist protocol.Methods:We enrolled 4743 IVF/ICSI cycles using flexible or fixed GnRH antagonist protocols between January 2017 and June 2021.The patients were stratified into four groups according to the LHLOT quartiles(Q1–Q4).Each group was further divided into two subgroups according to the median basal LH/LHLOT.The main outcomes were clinical pregnancy,birth,and miscarriage rates.Secondary outcomes were oocyte yield,number of MII,fertilized oocytes(2PN),and high-quality embryos.Results:Serum LHLOT levels were negatively correlated with oocyte yield and the numbers of MII,2PN,and high-quality embryos.Reproductive outcomes were compared among the different quartiles and subgroups.The absolute difference in the clinical pregnancy rates between the Q4 and Q1 groups was 6.4%(47.2%vs.40.8%,P<0.05).Clinical pregnancy rates between the subgroups with basal LH/LHLOT>3.04 and≤3.04 were 32.7%and 48.6%(P<0.01),respectively,in Q1.Within Q3,the clinical pregnancy rates between the subgroups with basal LH/LHLOT>2.14 and≤2.14 were 50.2%and 41.9%,respectively(P<0.05).The greater the decrease in LH levels,the lower the oocyte maturation rate across all quartiles.Conclusions:According to the LHLOT quartiles,the Q1 of serum LHLOT<1.43 IU/L may result in a higher number of oocytes but reduced oocyte maturation and lower clinical pregnancy rates.A decrease in LH levels by approximately 2.14 to 3.04 times compared to basal LH levels may lead to higher clinical pregnancy rates in IVF/ICSI patients following the GnRH antagonist protocol. 展开更多
关键词 GnRH antagonist IVF/ICSI luteinizing hormone Ovarian stimulation Reproductive outcomes
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Hormonal Status Assessment of Infertile Congolese Men
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作者 Constantin Moukouma Henriette Poaty +4 位作者 Rancia Colombe Diakouka Diambalou Ulrich Ngoma-Nzaou Guy Emergence Poaty Etienne Mokondjimobé Anani Wencesl Sévérin Odzebé 《Advances in Reproductive Sciences》 CAS 2024年第2期155-164,共10页
Background: The pathologies causing male infertility are various (congenital or acquired) and concern several hormone-producing organs: the hypothalamus, pituitary gland, testes and adrenals. The hormonal dosage inclu... Background: The pathologies causing male infertility are various (congenital or acquired) and concern several hormone-producing organs: the hypothalamus, pituitary gland, testes and adrenals. The hormonal dosage includes systematically testosterone, FSH and LH. These analyses often highlight hypogonadism hypogonadotropic or hypergonadotropic. They can sometimes be normal. The present study aimed to establish the hormonal profile of infertile men residing in Brazzaville. Patients and Methods: It was a cross-sectional descriptive study during five years (from 2018 to 2023). It concerned 344 infertilemen with an abnormal spermogram and spermocytogram. The method was performed from the blood dosage of testosterone, FSH, and LH using the Elisa technique. Results: The hormonal assessment was not normal in 48.83% (168/344) of cases. Increased values of testosterone were noted in 6.10% of cases and decreased in 12.20%. Abnormal high values of FSH were observed in 14.24% of cases and decreased values in 8.13%. LH was elevated, i.e. 2.03% of cases and decreased in 6.10% of subjects. Conclusions: A hormonal disturbance was observed in almost half of the infertile men. It concerned the hypothalamus-pituitary and the testes axis and indicated mainly a state of hypogonadism with high FSH and low LH. 展开更多
关键词 Male infertility hormoneS TESTOSTERONE FSH lh HYPOGONADISM
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基础黄体生成素和不同LH/FSH比值水平对PCOS患者辅助生殖妊娠结局的影响
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作者 叶子 王菁 +1 位作者 刘嘉茵 马翔 《实用妇产科杂志》 CAS CSCD 北大核心 2024年第3期213-218,共6页
目的:探究基础黄体生成素(bLH)及不同黄体生成素/卵泡刺激素(LH/FSH)比值水平对多囊卵巢综合征(PCOS)患者体外受精或卵胞浆内单精子注射(IVF/ICSI)妊娠结局的影响。方法:选择2013年7月至2020年1月在南京医科大学第一附属医院生殖医学科... 目的:探究基础黄体生成素(bLH)及不同黄体生成素/卵泡刺激素(LH/FSH)比值水平对多囊卵巢综合征(PCOS)患者体外受精或卵胞浆内单精子注射(IVF/ICSI)妊娠结局的影响。方法:选择2013年7月至2020年1月在南京医科大学第一附属医院生殖医学科首次行促性腺激素释放激素(GnRH)拮抗剂灵活方案促排卵,接受IVF/ICSI治疗的424例PCOS患者的临床资料进行回顾性分析。按照bLH水平不同分为正常bLH组(LH≤10 U/L,316例)和高bLH组(LH>10 U/L,108例);同时根据LH/FSH比值水平不同分为低比值组(LH/FSH≤1,227例)、中比值组(1<LH/FSH<2,142例)、高比值组(LH/FSH≥2,55例)。分析比较各组患者一般情况、促排卵情况及妊娠结局的差异,并采用二元Logistic回归分析影响活产的相关因素。结果:①高bLH组基础FSH和抗苗勒管激素(AMH)较正常bLH组高,差异有统计学意义(P<0.05)。在促排卵情况方面,高bLH组使用拮抗剂总天数长于正常组,但获卵数和绒促性素(HCG)日子宫内膜厚度小于正常bLH组,差异有统计学意义(P<0.05)。两组患者妊娠结局相关指标比较,差异无统计学意义(P>0.05)。②不同LH/FSH比值组患者比较,高比值组AMH和窦卵泡数(AFC)高于其他两组,差异有统计学意义(P<0.05)。在促排卵情况方面,高比值组获卵数、移植胚胎数、HCG日子宫内膜厚度均低于其他两组,差异有统计学意义(P<0.05)。3组患者妊娠结局相关指标比较,差异无统计学意义(P>0.05)。③二元Logistic回归分析显示活产与bLH、LH/FSH比值无关(P>0.05),与移植胚胎数有关(P<0.05)。结论:bLH水平和LH/FSH值升高可能会影响PCOS患者促排卵结果(如获卵数和HCG日子宫内膜厚度等),但与辅助生殖的活产率未见显著相关性。 展开更多
关键词 黄体生成素 卵泡刺激素 多囊卵巢综合征 辅助生殖 妊娠结局
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醋酸亮丙瑞林联合腹腔镜子宫肌瘤剔除术对子宫肌瘤FSH、E_(2)及LH水平的影响
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作者 许章山 《中国现代药物应用》 2024年第21期113-116,共4页
目的对子宫肌瘤患者实施醋酸亮瑞林、腹腔镜子宫肌瘤剔除术(LSM)联合方案,观察该联合方案对卵泡刺激素(FSH)、雌二醇(E_(2))、促黄体生成素(LH)水平的影响。方法选取100例子宫肌瘤患者,根据治疗方案的个体差异性分为对照组(LSM治疗)、... 目的对子宫肌瘤患者实施醋酸亮瑞林、腹腔镜子宫肌瘤剔除术(LSM)联合方案,观察该联合方案对卵泡刺激素(FSH)、雌二醇(E_(2))、促黄体生成素(LH)水平的影响。方法选取100例子宫肌瘤患者,根据治疗方案的个体差异性分为对照组(LSM治疗)、观察组(醋酸亮丙瑞林+LSM治疗),各50例。比较两组血清性激素水平、术后月经指标及围术期相关治疗指标。结果治疗前,对照组血清FSH、E_(2)、LH水平分别为(27.57±3.47)U/L、(275.39±33.48)pmol/L、(20.91±2.51)U/L,观察组分别为(27.61±3.36)U/L、(276.82±33.51)pmol/L、(20.85±2.49)U/L;治疗后,对照组血清FSH、E_(2)、LH水平分别为(18.17±2.84)U/L、(216.17±26.57)pmol/L、(17.49±1.85)U/L,观察组分别为(13.05±2.29)U/L、(139.84±18.79)pmol/L、(11.67±1.36)U/L。与治疗前相比,两组血清FSH、E_(2)、LH水平均明显下降,组内差异有统计学意义(P<0.05);观察组治疗后血清FSH、E_(2)、LH水平均低于对照组,组间差异有统计学意义(P<0.05)。观察组术后月经周期时间(29.56±1.32)d短于对照组的(33.13±2.29)d,月经量(41.33±4.32)ml少于对照组的(55.45±6.69)ml,组间差异有统计学意义(P<0.05)。观察组术中出血量、盆腔引流量少于对照组,下床活动时间、住院时间短于对照组,疾病复发率低于对照组,组间差异有统计学意义(P<0.05)。结论醋酸亮丙瑞林联合LSM运用于子宫肌瘤临床治疗过程中,可明显降低患者FSE、E_(2)、LH水平,减少术中出血量与盆腔引流量,加快术后身体恢复,降低疾病复发率,值得推荐。 展开更多
关键词 子宫肌瘤 醋酸亮丙瑞林 腹腔镜子宫肌瘤剔除术 卵泡刺激素 雌二醇 促黄体生成素
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抑制素A对牦牛颗粒细胞FSH、LH及其受体表达的影响 被引量:1
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作者 王立斌 王军乾 +5 位作者 赵凌 黄振华 王萌 王靖雷 余四九 潘阳阳 《核农学报》 CAS CSCD 北大核心 2023年第1期51-59,共9页
为了探明抑制素A(INHA)对牦牛卵泡颗粒细胞中促卵泡素(FSH)和促黄体素(LH)与其受体结合发挥的作用,通过体外培养牦牛卵泡颗粒细胞,采用免疫荧光技术(IF)检测促卵泡素受体(FSHR)和促黄体素受体(LHR)在颗粒细胞中的分布情况,用不同浓度外... 为了探明抑制素A(INHA)对牦牛卵泡颗粒细胞中促卵泡素(FSH)和促黄体素(LH)与其受体结合发挥的作用,通过体外培养牦牛卵泡颗粒细胞,采用免疫荧光技术(IF)检测促卵泡素受体(FSHR)和促黄体素受体(LHR)在颗粒细胞中的分布情况,用不同浓度外源性INHA(0、1.25、2.5、5、10、20 ng·mL^(-1))作用12 h后,利用实时荧光定量PCR(qRT-PCR)技术检测颗粒细胞中FSHβ、LHβ、FSHR、LHR基因的表达情况,采用酶联免疫分析试验(ELISA)检测细胞内外FSH、LH的含量。结果显示,FSHR和LHR在颗粒细胞质与细胞核中均有表达。FSHβ和LHβ基因在颗粒细胞中的表达随INHA浓度的增大呈先降后升的趋势;INHA浓度接近5 ng·mL^(-1)时,FSHβ表达最低;INHA浓度接近10 ng·mL^(-1)时,LHβ表达最低;FSHR和LHR基因的表达与INHA浓度呈负相关。INHA浓度接近5 ng·mL^(-1)时,颗粒细胞内外FSH含量最低;INHA浓度接近10 ng·mL^(-1)时,颗粒细胞内外LH含量最低;其他INHA浓度下,当颗粒细胞内容物中FSH和LH含量降低时,细胞上清中含量升高。综上所述,INHA对FSHR和LHR基因在颗粒细胞中的表达存在明显的抑制作用,并且影响FSHβ和LHβ基因在颗粒细胞中的表达。研究表明INHA可以抑制牦牛卵泡颗粒细胞中FSH、LH与其受体的结合;同时,INHA对颗粒细胞内外FSH和LH的含量具有重要调节作用。本研究为进一步探索抑制素对雌性牦牛生殖调控的影响提供了理论依据。 展开更多
关键词 颗粒细胞 抑制素A(INHA) 促卵泡素(FSH) 促黄体素(lh) 受体
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中枢性性早熟及外周性性早熟女童的LH、LHRH水平变化及临床意义 被引量:6
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作者 钟剑 魏苗苗 陈书远 《海南医学》 CAS 2023年第3期364-367,共4页
目的研究中枢性性早熟及外周性性早熟女童的黄体生成素(LH)、黄体生成素释放激素(LHRH)水平变化及临床意义。方法回顾性分析2018年1月至2021年12月在惠州市第一人民医院儿科诊治的54例性早熟女童的临床资料,按照早熟性质分为中枢性组(... 目的研究中枢性性早熟及外周性性早熟女童的黄体生成素(LH)、黄体生成素释放激素(LHRH)水平变化及临床意义。方法回顾性分析2018年1月至2021年12月在惠州市第一人民医院儿科诊治的54例性早熟女童的临床资料,按照早熟性质分为中枢性组(中枢性性早熟,35例)与外周性组(外周性性早熟,19例)。采用化学发光免疫法检测所有女童的LH、睾酮(T)、雌二醇(E2)、卵泡刺激素(FSH)水平及LHRH激发试验中的LH、FSH水平,计算并比较两组女童的骨龄指数、子宫容积和卵巢容积。结果中枢性组女童的LH、FSH水平分别为(4.35±1.54)IU/L、(7.28±2.11)IU/L,明显高于外周性组的(2.34±1.03)IU/L、(4.60±1.32)IU/L,T水平为(12.41±19.22)μg/L,明显低于外周性组的(84.46±123.24)μg/L,差异均有统计学意义(P<0.05);中枢性组女童的E2水平为(16.73±23.60)ng/L,与外周性组的(32.24±42.24)ng/L比较差异无统计学意义(P>0.05);LHRH激发试验分别30 min、60 min、90 min,中枢性组女童的LH水平分别为(19.53±2.66)IU/L、(16.15±2.12)IU/L、(12.45±1.86)IU/L,均明显高于外周性组的(5.65±1.78)IU/L、(5.38±1.40)IU/L、(4.26±1.17)IU/L,差异均有统计学意义(P<0.05);LHRH激发试验分别30 min、60 min、90 min,中枢性组女童的FSH水平分别为(15.24±5.28)IU/L、(15.90±5.06)IU/L、(15.99±5.40)IU/L,均明显高于外周性组的(10.96±3.27)IU/L、(11.23±3.74)IU/L、(10.12±3.21)IU/L,差异均有统计学意义(P<0.05);两组女童的BAI、子宫容积和卵巢容积比较差异均无统计学意义(P>0.05)。结论中枢性性早熟女童的LH、LHRH水平明显高于外周性性早熟女童,能够通过LH、LHRH水平对两者进行区分。 展开更多
关键词 中枢性性早熟 外周性性早熟 黄体生成素 黄体生成素释放激素 临床意义
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