Hepatoprotective activity of Dypsis lutescens against D-galactosamine-induced hepatotoxicity in rats and its phytoconstituents

Objective:To isolate and identify the polyphenolic constituents of Dypsis lutescens,and evaluate the hepatoprotective activity of the ethanolic extract of Dypsis lutescens leaves.Methods:Hepatoprotective,antioxidant and anti-inflammatory effects of two doses of Dypsis lutescens ethanolic leaf extract were investigated in five groups of six rats each administered with the ethanolic extract of Dypsis lutescens leaves.Liver function parameters were assessed,histopathological study was carried out,the anti-inflammatory mediators and the antioxidant potential in the liver tissues were evaluated.In addition,the total ethanolic extract of Dypsis lutescens leaves was subjected to different chromatographic separation techniques to yield ten phenolic compounds.The isolated compounds structures were spectroscopically elucidated.Results:Hepatoprotective activity of Dypsis lutescens ethanolic extract was estimated for the first time and showed significant activity against histopathological changes induced by D-galactosamine in liver.The extract improved the liver functions.Compared to the D-galactosamine group,the architecture of the liver in the treated groups was improved in the histopathological examination.These results proved the hepatoprotective activity of Dypsis lutescens and its ability in attenuating liver oxidative damage and inflammation.Phytochemical investigations of the total extract afforded ten compounds from the genus Dypsis.Conclusions:The alcoholic extract of Dypsis lutescens exerted potential hepatoprotective action,maintaining liver health and functions.

Bioecology of Phonoctonus lutescens(Guérin Meneville and Percheron)Predator of Dysdercus voëlkeri(Schmidt,1932),Feeding on Dysdercus voëlkeri in the Laboratory Conditions in Burkina Faso

The study was conducted at the agricultural experimental station of Farako-Bâ, specifically in the Cotton Program. Insects were collected in Farako-Bâ?field and raised in the Cotton Program. Dysdercus voëkeri Schmidt is one of cotton cultivation main pests in Burkina Faso. The control of this devastating cotton bug is based on chemical using. For researching alternative solutions, a part of the biological control method was investigated by using Phonoctonus lutescens which is D. vöelkeri natural enemy, in order to develop a biological control method. To understand the bioecology of P. lutescens, our study has been carried out on this insect under laboratory conditions when it was feed on its prey which is D. voëkeri. The results have demonstrated that the pre-copulation period is 9.33 ± 2.14 days. The oviposition period is 6.97 ± 1.47 days, after which 366.73 ± 27.43 eggs on average are laid with 92.33% ± 4% hatchability. From hatching to adult stage, P. lutescens larvae development goes through five stages with variable durations according to the stage. The results showed that the development cycle lasted 57.23 ± 5.81 days at a temperature of 27.5°C ± 2°C and a relative humidity of 42% ± 3%. Survival rates ranged from 92% to 97.47%. Males and females lived respectively 87.5 ± 27.99 days and 107.97 ± 24.21 days. These results could permit a better use of P. lutescens through a mass rearing and an optimization of D. voëkeri biological control.