Objective: To explore the related factors of surgical treatment of patients with corpus luteum rupture and establish a risk prediction model of surgical treatment of corpus luteum rupture. Methods: 222 patients with c...Objective: To explore the related factors of surgical treatment of patients with corpus luteum rupture and establish a risk prediction model of surgical treatment of corpus luteum rupture. Methods: 222 patients with corpus luteum rupture treated in Jingzhou First People’s Hospital from January 2015 to March 2022 were analyzed retrospectively, including 45 cases of surgery and 177 cases of conservative treatment. The training set and validation set were randomly assigned according to 7:3. We collected the basic information, laboratory and ultrasonic examination data of 222 patients. Logistic regression analysis was used to determine the independent risk factors and combined predictors of surgical treatment of corpus luteum rupture. The risk prediction model was established and the nomogram was drawn. The discrimination and calibration of the prediction model were verified and evaluated by receiver operating characteristic (ROC) curve, calibration curve and Hosmer-Lemeshow goodness of fit test;Decision curve analysis (DCA) was used to evaluate the clinical effectiveness of the prediction model. Results: Univariate logistic regression showed that whole abdominal pain (OR: 2.314, 95% CI: 1.090 - 4.912), abdominal muscle tension (OR: 2.379, 95% CI: 1.112 - 5.089), adnexal mass ≥ 4 cm (OR: 3.926, 95% CI: 1.771 - 8.266), hemoglobin Conclusion: The nomogram prediction model containing three predictive variables (hemoglobin, depth of pelvic effusion under ultrasound and cervical lifting pain) can be used to predict the risk of surgical treatment in patients with corpus luteum rupture.展开更多
Curtobacterium luteum, a gram-positive psychrotrophic bacterium, secreting an extracellular protease was isolated from the soil of Gangotri glacier, Western Himalaya. The maximum enzyme production was achieved when is...Curtobacterium luteum, a gram-positive psychrotrophic bacterium, secreting an extracellular protease was isolated from the soil of Gangotri glacier, Western Himalaya. The maximum enzyme production was achieved when isolate was grown in a pH-neutral medium containing skim milk at 15oC over 120 hour. The metal ions such as Zn2+ and Cr2+ enhanced enzyme production. The specific activity of purified enzyme was 8090 u/mg after 34.1 fold purification. The 115 kD enzyme was a metalloprotease (activity inhibited by EDTA and EGTA) and showed maximum activity at 20oC and pH 7. The enzyme was active over a broad pH range and retained 84% of its original activity between pH 6–8. There was no loss in enzyme activity when exposed for 3 hours at 4oC-20oC. However, lost 65% of activity at 30oC, and was almost inactivated at 50oC, but was resistant to repeated freezing and thawing. The enzyme activity was stimulated by manganese ions; however, it was inactivated by copper ions.展开更多
Aim: To investigate the efficacy of two corpus luteum support programs in patients with frozen embryo transfer (FET). Methods: A retrospective analysis of the complete clinical data of 340 patients undergoing in vitro...Aim: To investigate the efficacy of two corpus luteum support programs in patients with frozen embryo transfer (FET). Methods: A retrospective analysis of the complete clinical data of 340 patients undergoing in vitro fertilization embryo transfer in the Department of Reproductive Medicine, Jingzhou Central Hospital from September 2016 to July 2018. These patients were divided into group A and B according to the luteal support program. We compared the clinical outcomes of the two corpus luteum preparations by comparing the laboratory parameters of the two groups of patients with clinical pregnancy indicators. Results: We found there was no significant difference in the results of general conditions in the two groups of patients, such as infertility age and duration, body mass index (BMI), basal follicle stimulating hormone (FSH), basal luteinizing hormone (LH), basal estrogen (E2) levels, the endometrial thickness, the number of transplanted high-quality embryos on the day of transplantation and so on (P > 0.05). We found that the implantation rate (32.55%), biochemical pregnancy rate (53.57%), and clinical pregnancy rate (51.78%) in group B were significantly higher than those in group A (25.26%, 35.11%, and 34.66%, respectively) (P 0.05). Conclusion: We conclude that patients who are scheduled for frozen embryo transfer use progesterone vaginal sustained release capsules combined with luteal progesterone luteal support, have improved clinical pregnancy rates.展开更多
Objective:To investigate the diagnostic effects of enhanced CT scan on hemorrhage corpus luteum cyst(HCLC).Methods:The clinical data of 24 patients with hemorrhage corpus luteum cyst diagnosed by surgery and pathology...Objective:To investigate the diagnostic effects of enhanced CT scan on hemorrhage corpus luteum cyst(HCLC).Methods:The clinical data of 24 patients with hemorrhage corpus luteum cyst diagnosed by surgery and pathology in our hospital were collected.All patients received enhanced CT scan to evaluate the diagnostic value of CT.Results:The average diameter of the cysts in this group of patients was 5.1cm by CT.Further detection of the ruptured cyst wall showed that there was a liquid lowdensity shadow.Some of the cysts were accompanied by signs of increased density,indicating the presence of blood clots;all patients received surgical treatment.Intraoperative ultrasound examination of the cysts had an average diameter of 5.2cm and a wall of 0.2~0.4mm.Among them,19 cases of cysts contained"coffee-colored"liquid with varying degrees of blood clots,and 5 cases had no blood clots in the cysts but there were a lot of blood clots beside the cysts.Conclusion:The application of enhanced CT scan in patients with hemorrhage corpus luteum cyst is of great value.It can provide accurate data reference for clinical treatment and is worthy of promoting its clinical application.展开更多
Sequential ultrasonograms of both ovaries were taken once a day in six postpartum Jersey cows with B-mode Ultrasound Scanner and the concentrations of progesterone in peripheral circulation of the cows were measured b...Sequential ultrasonograms of both ovaries were taken once a day in six postpartum Jersey cows with B-mode Ultrasound Scanner and the concentrations of progesterone in peripheral circulation of the cows were measured by radioimmunoassay (RIA). The whole texture of the previous pregnant corpus luteum was clearly identified during the process of its regressing. The ppCL could be identified for a mean of 17±12.96 days with a range of 4 to 42 days. There were no significant correlation between the time that the ppCL could be identified and both the times to the first postpartum ovulation and the peripheral progesterone concentration.展开更多
文摘Objective: To explore the related factors of surgical treatment of patients with corpus luteum rupture and establish a risk prediction model of surgical treatment of corpus luteum rupture. Methods: 222 patients with corpus luteum rupture treated in Jingzhou First People’s Hospital from January 2015 to March 2022 were analyzed retrospectively, including 45 cases of surgery and 177 cases of conservative treatment. The training set and validation set were randomly assigned according to 7:3. We collected the basic information, laboratory and ultrasonic examination data of 222 patients. Logistic regression analysis was used to determine the independent risk factors and combined predictors of surgical treatment of corpus luteum rupture. The risk prediction model was established and the nomogram was drawn. The discrimination and calibration of the prediction model were verified and evaluated by receiver operating characteristic (ROC) curve, calibration curve and Hosmer-Lemeshow goodness of fit test;Decision curve analysis (DCA) was used to evaluate the clinical effectiveness of the prediction model. Results: Univariate logistic regression showed that whole abdominal pain (OR: 2.314, 95% CI: 1.090 - 4.912), abdominal muscle tension (OR: 2.379, 95% CI: 1.112 - 5.089), adnexal mass ≥ 4 cm (OR: 3.926, 95% CI: 1.771 - 8.266), hemoglobin Conclusion: The nomogram prediction model containing three predictive variables (hemoglobin, depth of pelvic effusion under ultrasound and cervical lifting pain) can be used to predict the risk of surgical treatment in patients with corpus luteum rupture.
文摘Curtobacterium luteum, a gram-positive psychrotrophic bacterium, secreting an extracellular protease was isolated from the soil of Gangotri glacier, Western Himalaya. The maximum enzyme production was achieved when isolate was grown in a pH-neutral medium containing skim milk at 15oC over 120 hour. The metal ions such as Zn2+ and Cr2+ enhanced enzyme production. The specific activity of purified enzyme was 8090 u/mg after 34.1 fold purification. The 115 kD enzyme was a metalloprotease (activity inhibited by EDTA and EGTA) and showed maximum activity at 20oC and pH 7. The enzyme was active over a broad pH range and retained 84% of its original activity between pH 6–8. There was no loss in enzyme activity when exposed for 3 hours at 4oC-20oC. However, lost 65% of activity at 30oC, and was almost inactivated at 50oC, but was resistant to repeated freezing and thawing. The enzyme activity was stimulated by manganese ions; however, it was inactivated by copper ions.
文摘Aim: To investigate the efficacy of two corpus luteum support programs in patients with frozen embryo transfer (FET). Methods: A retrospective analysis of the complete clinical data of 340 patients undergoing in vitro fertilization embryo transfer in the Department of Reproductive Medicine, Jingzhou Central Hospital from September 2016 to July 2018. These patients were divided into group A and B according to the luteal support program. We compared the clinical outcomes of the two corpus luteum preparations by comparing the laboratory parameters of the two groups of patients with clinical pregnancy indicators. Results: We found there was no significant difference in the results of general conditions in the two groups of patients, such as infertility age and duration, body mass index (BMI), basal follicle stimulating hormone (FSH), basal luteinizing hormone (LH), basal estrogen (E2) levels, the endometrial thickness, the number of transplanted high-quality embryos on the day of transplantation and so on (P > 0.05). We found that the implantation rate (32.55%), biochemical pregnancy rate (53.57%), and clinical pregnancy rate (51.78%) in group B were significantly higher than those in group A (25.26%, 35.11%, and 34.66%, respectively) (P 0.05). Conclusion: We conclude that patients who are scheduled for frozen embryo transfer use progesterone vaginal sustained release capsules combined with luteal progesterone luteal support, have improved clinical pregnancy rates.
文摘Objective:To investigate the diagnostic effects of enhanced CT scan on hemorrhage corpus luteum cyst(HCLC).Methods:The clinical data of 24 patients with hemorrhage corpus luteum cyst diagnosed by surgery and pathology in our hospital were collected.All patients received enhanced CT scan to evaluate the diagnostic value of CT.Results:The average diameter of the cysts in this group of patients was 5.1cm by CT.Further detection of the ruptured cyst wall showed that there was a liquid lowdensity shadow.Some of the cysts were accompanied by signs of increased density,indicating the presence of blood clots;all patients received surgical treatment.Intraoperative ultrasound examination of the cysts had an average diameter of 5.2cm and a wall of 0.2~0.4mm.Among them,19 cases of cysts contained"coffee-colored"liquid with varying degrees of blood clots,and 5 cases had no blood clots in the cysts but there were a lot of blood clots beside the cysts.Conclusion:The application of enhanced CT scan in patients with hemorrhage corpus luteum cyst is of great value.It can provide accurate data reference for clinical treatment and is worthy of promoting its clinical application.
文摘Sequential ultrasonograms of both ovaries were taken once a day in six postpartum Jersey cows with B-mode Ultrasound Scanner and the concentrations of progesterone in peripheral circulation of the cows were measured by radioimmunoassay (RIA). The whole texture of the previous pregnant corpus luteum was clearly identified during the process of its regressing. The ppCL could be identified for a mean of 17±12.96 days with a range of 4 to 42 days. There were no significant correlation between the time that the ppCL could be identified and both the times to the first postpartum ovulation and the peripheral progesterone concentration.