BACKGROUND Micrococcus luteus(M.luteus)-induced endophthalmitis is very rare and and may present as either acute or chronic postoperative endophthalmitis.The aim of this study was to report a case of delayed-onset M.l...BACKGROUND Micrococcus luteus(M.luteus)-induced endophthalmitis is very rare and and may present as either acute or chronic postoperative endophthalmitis.The aim of this study was to report a case of delayed-onset M.luteus-induced endophthalmitis that occurred several months after cataract surgery.CASE SUMMARY A 78-year-old man presented with decreased vision,pain and redness in the right eye that had begun 3 days prior.He had undergone cataract surgery 4 mo prior.Visual acuity was counting fingers;slit-lamp examination revealed conjunctival injection,posterior corneal precipitates,anterior chamber inflammation(cell 4+),and hypopyon.Fundus examination revealed moderate vitreous haze.Urgent vitrectomy was performed for suspected infectious endophthalmitis,followed by vitreous irrigation with injections of antibiotics.On the postoperative day 1,anterior chamber cell decreased to 2+and hypopyon was not observed on slit lamp examination.Six days postoperatively,the patient had recurrent eye pain,and the anterior chamber cell grade increased to 4+;hypopyon recurred in the anterior chamber,and whitish plaque was observed in the lens capsule.Therefore,the patient underwent intraocular lens(IOL)and lens capsule removal,followed by vitreous irrigation,antibiotics injection,and vitrectomy.M.luteus was identified from a lens capsule culture.CONCLUSION In cases of delayed-onset M.luteus-induced endophthalmitis,early vitrectomy and removal of the IOL and lens capsule may be necessary.展开更多
Korean spruce （Picea koraiensis Sieb. E1 Zucc.） is one of the main afforestation species in northern China. Seedling quality is a critical factor at planting time. To test whether the synergistic growth enhancement ...Korean spruce （Picea koraiensis Sieb. E1 Zucc.） is one of the main afforestation species in northern China. Seedling quality is a critical factor at planting time. To test whether the synergistic growth enhancement of Scotch pine （P. sylvestris var. mongolica） seedlings brought by the plant beneficial fungus Trichoderma virens （J.H. Mill., Giddens and A.A. Foster） Arx and ectomycorrhizal fungus （Suillus luteus （L.） Roussel.） can also benefit Korean spruce seed- lings, we examined the effects of S. luteus and T. virens on the growth of P. koraiensis seedlings and drought resistance of P. sylvestris var. mongolica in peat soils. The two fungi were added to sterilized peat soil in pots, and the plants were grown for 4 months. Seedling growth and physiological variables, including mycorrhizal colonization rate of roots, biomass, and chlorophyll content, were examined. The colonization rate of the mycorrhizal fungus on P. koraiensis exceeded 65 %, and the synergism between S. luteus and T. virens enhanced most of the variables for P. koraiensis seedlings after inoculation with S. luteus then 30 days later with T. virens as in our published results for seedlings of P. sylvestris var. mongolica. When seedlings of P. sylvestris var. mongolica were inoculated with this sequence, they became more drought tolerant. T. virens also induced S. luteus to produce -l,3-glucanase and chitinase. This inocu- lation sequence at planting can thus improve the quality of P. sylvestris var. mongolica and P. koraiensis seedlings and substantiates our previous results.展开更多
The alimentary canal contents of 360 Barbus luteus caught from MOD between January 2010 to December 2010 were examined. The phyto-plankton, zooplanktons, detritus, diatoms, insects and unidentified digestive food were...The alimentary canal contents of 360 Barbus luteus caught from MOD between January 2010 to December 2010 were examined. The phyto-plankton, zooplanktons, detritus, diatoms, insects and unidentified digestive food were identified in the alimentary canal of fish. The results of diet analysis showed that the B. luteus was omnivorous. The species of fish tended to consume more plant origin food. The aquatic plants and tissues were the main part of thier food followed by chlorophyta and cyanophyta. The results revealed that the species obtained or fell their food item from the bed of rivers or near to it.展开更多
Micrococcus luteus synthesises glucose and protein during the fermentation of a Nigerian rice. To regulate the formation of these substances, mutation was carried out with an alkylating agent: ethylmethyl sulphonate (...Micrococcus luteus synthesises glucose and protein during the fermentation of a Nigerian rice. To regulate the formation of these substances, mutation was carried out with an alkylating agent: ethylmethyl sulphonate (EMS). Screening the mutants generated for the levels of the traits expressed, four major groups were obtained. These are poor, moderate, good and super producers of either glucose or protein. They produced the properties at 0 - 1.00, 1.01 to 1.99 (moderate) and 2.0 to 2.99 (good) and 3.0 and above (super) mg.mL–1 of each substance. The classes were made up of 37, 40, 20 and 3 mutants for glucose production and 13, 35, 40 and 12 mutants for protein synthesis. The wild strain bacterium made 0.86 mg.mL–1 glucose and 1.2 mg.mL–1 protein describing the M. luteus as poor glucose maker and moderate protein producer. It was also noticed that the mutation caused some variants (25%) to form more glucose than protein;the remaining 75% of the population are made up of two sets viz: mutants having better ability to synthesise protein at higher concentrations than glucose and those that formed about the same amounts of the substances. It thus follows that the glucose and protein productions in M. luteus are genetically based and can be regulated by genetic manipulation.展开更多
In this study,pot experiments were conducted on the seedlings of Pinus sylvestris var.mongolica to study the influence of Trichoderma(Trichoderma harzianum E15)and Ectomycorrhizal fungi(Suillus luteus N94)on the growt...In this study,pot experiments were conducted on the seedlings of Pinus sylvestris var.mongolica to study the influence of Trichoderma(Trichoderma harzianum E15)and Ectomycorrhizal fungi(Suillus luteus N94)on the growth of these seedlings.In particular,the effects of these fungi on the fungal community structure in the rhizosphere soil of the seedlings were investigated.Inoculation with Trichoderma harzianum E15 and Suillus luteus N94 significantly(P<0.05)promoted the growth of the Pinus sylvestris seedlings.The non-metric multidimensional scaling(NMDS)results indicated a significant difference(P<0.05)between the fungal community structures in the rhizosphere soil of the annual and biennial seedlings.In the rhizosphere soil of annual seedlings,the main fungi were Ascomycota,Basidiomycota,Zygomycota.Ascomycota,Basidiomycota,Mortierellomycota,and p-unclassified-k-Fungi were the main fungi in the rhizosphere soil of biennial seedlings.The dominant genus in the rhizosphere soil and a key factor promoting the growth of the annual and the biennial seedlings was Trichoderma,Suillus,respectively.Both of them were negatively correlated with the relative abundance of microbial flora in the symbiotic environment.Trichoderma had a significant promoting effect on the conversion of total phosphorus,total nitrogen,ammonium nitrogen,nitrate nitrogen,and the organic matter in the rhizosphere soil of the seedlings,while Suillus significantly promoted the conversion of organic matter and total phosphorus.展开更多
Mongolian pine is an important afforestation species widely used for ecological management in northeast China. The environment in this region is very unstable and the flora are regularly subjected to drought stress.Th...Mongolian pine is an important afforestation species widely used for ecological management in northeast China. The environment in this region is very unstable and the flora are regularly subjected to drought stress.This paper reports on the influence of inoculation with the Suillus luteus on seedlings under different water conditions.Both inoculated and non-inoculated ectomycorrhizal fungi(ECMF)-S. luteus seedlings were maintained under wellwatered or water-stress conditions for 3 months. The S.luteus colonization rate under water stress was higher than that in well-watered conditions. Under water stress, inoculated seedlings had greater growth than non-inoculated seedlings. In addition, under water stress, S. luteus-inoculated seedlings had greater superoxide dismutase and peroxidase activity, higher soluble protein content, lower proline content, and lower malondialdehyde content than non-inoculated seedlings. S. luteus colonization increased the rhizosphere soil-enzyme activity and the rhizosphere soil nutrition content under both well-watered and water-stress conditions. Given the positive impact on seedling growth and physiology, S. luteus shows potential for use in the arid and semi-arid regions of northeast China for afforestation.展开更多
Interleukin-17(IL-17),a prototype member of IL-17 family,plays an important role in defending against extracellular pathogens as a pro-inflammatory cytokine.The function and distribution of IL-17 have been extensively...Interleukin-17(IL-17),a prototype member of IL-17 family,plays an important role in defending against extracellular pathogens as a pro-inflammatory cytokine.The function and distribution of IL-17 have been extensively studied in many vertebrates.However,few study has focused on the role of IL-17 in invertebrates,especially in mollusks.In this study,an IL-17 homolog was identified in Octopus ocellatus,which was designated as OoIL-17.The phylogenetic analysis showed that OoIL-17 is clustered well with other invertebrate IL-17,indicating it is highly similar with the IL-17 of other invertebrates.The expression of OoIL-17 gene was analyzed with qRT-PCR in a variety of healthy tissues and the hemocytes infected with Vibro anguillarum or Micrococcus luteus.The mRNA of OoIL-17 gene is constitutively expressed at different levels in all examined tissues of healthy O.ocellatus,including mantle,stomach,hemocytes,muscle,gonad,hepatopancreas,systemic heart and gill.The lowest expression was observed in mantle while the highest was observed in hepatopancreas.The expression level of OoIL-17 gene is significantly up-regulated in O.ocellatus hemocytes upon infection with V.anguillarum and M.luteus,indicating its active involvement in the host immune response against bacterial pathogens.The results laid the foundation for further understanding the innate immune mechanisms of IL-17 in O.ocellatus and mollusks.展开更多
Background： Early embryonic developmental arrest is the most commonly understudied adverse outcome of pregnancy. The relevance of intrauterine infection to spontaneous embryonic death is rarely studied and remains un...Background： Early embryonic developmental arrest is the most commonly understudied adverse outcome of pregnancy. The relevance of intrauterine infection to spontaneous embryonic death is rarely studied and remains unclear. This study aimed to investigate the relationship between intrauterine bacterial infection and early embryonic developmental arrest. Methods： Embryonic chorion tissue and uterine swabs for bacterial detection were obtained from 33 patients who underwent artificial abortion （control group） and from 45 patients who displayed early embryonic developmental arrest （trial group）. Results： Intrauterine bacterial infection was discovered in both groups. The infection rate was 24.44% （11/45） in the early embryonic developmental arrest group and 9.09% （3/33） in the artificial abortion group. Classification analysis revealed that the highest detection rate for Micrococcus luteus in the early embryonic developmental arrest group was 13.33% （6/45）, and none was detected in the artificial abortion group. M. luteus infection was significantly different between the groups （P 〈 0.05 as shown by Fisher＇s exact test）. In addition, no correlation was found between intrauterine bacterial infection and history of early embryonic developmental arrest. Conelusions： IV/. luteus infection is related to early embryonic developmental arrest and might be one of its causative factors.展开更多
We evaluated the attraction ofMonochamus alternatus Hope （Coleoptera： Cerambycidae）, Dryocoetes luteus Blandford and Orthotomicus erosus Wollaston （Coleoptera： Curculionidae： Scolytinae） to multiple-funnel trap...We evaluated the attraction ofMonochamus alternatus Hope （Coleoptera： Cerambycidae）, Dryocoetes luteus Blandford and Orthotomicus erosus Wollaston （Coleoptera： Curculionidae： Scolytinae） to multiple-funnel traps baited with the pine volatiles, ethanol and （＋）-α-pinene and the bark beetle pheromones, ipsenol and ipsdienol. M. alternatus were attracted to traps baited with ethanol and （＋）-α-pinene but not those baited with ipsdienol and ipsenol. Ipsdienol and ipsenol decreased catches ofM. alternatus in traps baited with ethanol and （＋）-α-pinene. Traps baited with either binary combinations of ethanol and （＋）-α-pinene or ipsdienol and ipsenol were attractive to D. luteus and O. erosus. The addition of ipsenol and ipsdienol to traps baited with ethanol and （＋）-α-pinene synergized attraction of O. erosus but not D. luteus .展开更多
文摘BACKGROUND Micrococcus luteus(M.luteus)-induced endophthalmitis is very rare and and may present as either acute or chronic postoperative endophthalmitis.The aim of this study was to report a case of delayed-onset M.luteus-induced endophthalmitis that occurred several months after cataract surgery.CASE SUMMARY A 78-year-old man presented with decreased vision,pain and redness in the right eye that had begun 3 days prior.He had undergone cataract surgery 4 mo prior.Visual acuity was counting fingers;slit-lamp examination revealed conjunctival injection,posterior corneal precipitates,anterior chamber inflammation(cell 4+),and hypopyon.Fundus examination revealed moderate vitreous haze.Urgent vitrectomy was performed for suspected infectious endophthalmitis,followed by vitreous irrigation with injections of antibiotics.On the postoperative day 1,anterior chamber cell decreased to 2+and hypopyon was not observed on slit lamp examination.Six days postoperatively,the patient had recurrent eye pain,and the anterior chamber cell grade increased to 4+;hypopyon recurred in the anterior chamber,and whitish plaque was observed in the lens capsule.Therefore,the patient underwent intraocular lens(IOL)and lens capsule removal,followed by vitreous irrigation,antibiotics injection,and vitrectomy.M.luteus was identified from a lens capsule culture.CONCLUSION In cases of delayed-onset M.luteus-induced endophthalmitis,early vitrectomy and removal of the IOL and lens capsule may be necessary.
基金the National Natural Science Foundation of China (31170597, 31200484)the National Fiveyear Science and Technology Research Project (2012BAD19B0801)+1 种基金the Fundamental Research Funds for the Central Universities (2572014AA30)the National Forestry Bureau ‘‘948’’ Project (2009-4-39) for financial support
文摘Korean spruce （Picea koraiensis Sieb. E1 Zucc.） is one of the main afforestation species in northern China. Seedling quality is a critical factor at planting time. To test whether the synergistic growth enhancement of Scotch pine （P. sylvestris var. mongolica） seedlings brought by the plant beneficial fungus Trichoderma virens （J.H. Mill., Giddens and A.A. Foster） Arx and ectomycorrhizal fungus （Suillus luteus （L.） Roussel.） can also benefit Korean spruce seed- lings, we examined the effects of S. luteus and T. virens on the growth of P. koraiensis seedlings and drought resistance of P. sylvestris var. mongolica in peat soils. The two fungi were added to sterilized peat soil in pots, and the plants were grown for 4 months. Seedling growth and physiological variables, including mycorrhizal colonization rate of roots, biomass, and chlorophyll content, were examined. The colonization rate of the mycorrhizal fungus on P. koraiensis exceeded 65 %, and the synergism between S. luteus and T. virens enhanced most of the variables for P. koraiensis seedlings after inoculation with S. luteus then 30 days later with T. virens as in our published results for seedlings of P. sylvestris var. mongolica. When seedlings of P. sylvestris var. mongolica were inoculated with this sequence, they became more drought tolerant. T. virens also induced S. luteus to produce -l,3-glucanase and chitinase. This inocu- lation sequence at planting can thus improve the quality of P. sylvestris var. mongolica and P. koraiensis seedlings and substantiates our previous results.
文摘The alimentary canal contents of 360 Barbus luteus caught from MOD between January 2010 to December 2010 were examined. The phyto-plankton, zooplanktons, detritus, diatoms, insects and unidentified digestive food were identified in the alimentary canal of fish. The results of diet analysis showed that the B. luteus was omnivorous. The species of fish tended to consume more plant origin food. The aquatic plants and tissues were the main part of thier food followed by chlorophyta and cyanophyta. The results revealed that the species obtained or fell their food item from the bed of rivers or near to it.
文摘Micrococcus luteus synthesises glucose and protein during the fermentation of a Nigerian rice. To regulate the formation of these substances, mutation was carried out with an alkylating agent: ethylmethyl sulphonate (EMS). Screening the mutants generated for the levels of the traits expressed, four major groups were obtained. These are poor, moderate, good and super producers of either glucose or protein. They produced the properties at 0 - 1.00, 1.01 to 1.99 (moderate) and 2.0 to 2.99 (good) and 3.0 and above (super) mg.mL–1 of each substance. The classes were made up of 37, 40, 20 and 3 mutants for glucose production and 13, 35, 40 and 12 mutants for protein synthesis. The wild strain bacterium made 0.86 mg.mL–1 glucose and 1.2 mg.mL–1 protein describing the M. luteus as poor glucose maker and moderate protein producer. It was also noticed that the mutation caused some variants (25%) to form more glucose than protein;the remaining 75% of the population are made up of two sets viz: mutants having better ability to synthesise protein at higher concentrations than glucose and those that formed about the same amounts of the substances. It thus follows that the glucose and protein productions in M. luteus are genetically based and can be regulated by genetic manipulation.
基金Project supported by the National Natural Science Foundation of China(No.81460648)the Program for Changjiang Scholars and Innovative Research Team in University(No.IRT13095),China
文摘In this study,pot experiments were conducted on the seedlings of Pinus sylvestris var.mongolica to study the influence of Trichoderma(Trichoderma harzianum E15)and Ectomycorrhizal fungi(Suillus luteus N94)on the growth of these seedlings.In particular,the effects of these fungi on the fungal community structure in the rhizosphere soil of the seedlings were investigated.Inoculation with Trichoderma harzianum E15 and Suillus luteus N94 significantly(P<0.05)promoted the growth of the Pinus sylvestris seedlings.The non-metric multidimensional scaling(NMDS)results indicated a significant difference(P<0.05)between the fungal community structures in the rhizosphere soil of the annual and biennial seedlings.In the rhizosphere soil of annual seedlings,the main fungi were Ascomycota,Basidiomycota,Zygomycota.Ascomycota,Basidiomycota,Mortierellomycota,and p-unclassified-k-Fungi were the main fungi in the rhizosphere soil of biennial seedlings.The dominant genus in the rhizosphere soil and a key factor promoting the growth of the annual and the biennial seedlings was Trichoderma,Suillus,respectively.Both of them were negatively correlated with the relative abundance of microbial flora in the symbiotic environment.Trichoderma had a significant promoting effect on the conversion of total phosphorus,total nitrogen,ammonium nitrogen,nitrate nitrogen,and the organic matter in the rhizosphere soil of the seedlings,while Suillus significantly promoted the conversion of organic matter and total phosphorus.
基金supported by the National Natural Science Foundation of China(31670649,31200484,31170597)
文摘Mongolian pine is an important afforestation species widely used for ecological management in northeast China. The environment in this region is very unstable and the flora are regularly subjected to drought stress.This paper reports on the influence of inoculation with the Suillus luteus on seedlings under different water conditions.Both inoculated and non-inoculated ectomycorrhizal fungi(ECMF)-S. luteus seedlings were maintained under wellwatered or water-stress conditions for 3 months. The S.luteus colonization rate under water stress was higher than that in well-watered conditions. Under water stress, inoculated seedlings had greater growth than non-inoculated seedlings. In addition, under water stress, S. luteus-inoculated seedlings had greater superoxide dismutase and peroxidase activity, higher soluble protein content, lower proline content, and lower malondialdehyde content than non-inoculated seedlings. S. luteus colonization increased the rhizosphere soil-enzyme activity and the rhizosphere soil nutrition content under both well-watered and water-stress conditions. Given the positive impact on seedling growth and physiology, S. luteus shows potential for use in the arid and semi-arid regions of northeast China for afforestation.
基金supported by the earmarked fund for the Modern Agro-Industry Technology Research System (No. CARS-49)the Natural Science Foundation of Shandong Province (No. ZR2019BC052)the Marine and Fisheries Science and Technology Innovation Program of Shan-dong Province (No. 2017YY04)
文摘Interleukin-17(IL-17),a prototype member of IL-17 family,plays an important role in defending against extracellular pathogens as a pro-inflammatory cytokine.The function and distribution of IL-17 have been extensively studied in many vertebrates.However,few study has focused on the role of IL-17 in invertebrates,especially in mollusks.In this study,an IL-17 homolog was identified in Octopus ocellatus,which was designated as OoIL-17.The phylogenetic analysis showed that OoIL-17 is clustered well with other invertebrate IL-17,indicating it is highly similar with the IL-17 of other invertebrates.The expression of OoIL-17 gene was analyzed with qRT-PCR in a variety of healthy tissues and the hemocytes infected with Vibro anguillarum or Micrococcus luteus.The mRNA of OoIL-17 gene is constitutively expressed at different levels in all examined tissues of healthy O.ocellatus,including mantle,stomach,hemocytes,muscle,gonad,hepatopancreas,systemic heart and gill.The lowest expression was observed in mantle while the highest was observed in hepatopancreas.The expression level of OoIL-17 gene is significantly up-regulated in O.ocellatus hemocytes upon infection with V.anguillarum and M.luteus,indicating its active involvement in the host immune response against bacterial pathogens.The results laid the foundation for further understanding the innate immune mechanisms of IL-17 in O.ocellatus and mollusks.
文摘Background： Early embryonic developmental arrest is the most commonly understudied adverse outcome of pregnancy. The relevance of intrauterine infection to spontaneous embryonic death is rarely studied and remains unclear. This study aimed to investigate the relationship between intrauterine bacterial infection and early embryonic developmental arrest. Methods： Embryonic chorion tissue and uterine swabs for bacterial detection were obtained from 33 patients who underwent artificial abortion （control group） and from 45 patients who displayed early embryonic developmental arrest （trial group）. Results： Intrauterine bacterial infection was discovered in both groups. The infection rate was 24.44% （11/45） in the early embryonic developmental arrest group and 9.09% （3/33） in the artificial abortion group. Classification analysis revealed that the highest detection rate for Micrococcus luteus in the early embryonic developmental arrest group was 13.33% （6/45）, and none was detected in the artificial abortion group. M. luteus infection was significantly different between the groups （P 〈 0.05 as shown by Fisher＇s exact test）. In addition, no correlation was found between intrauterine bacterial infection and history of early embryonic developmental arrest. Conelusions： IV/. luteus infection is related to early embryonic developmental arrest and might be one of its causative factors.
基金Acknowledgments This work was funded by the State Key Laboratory of Integrated Management of Pest Insects and Rodents （Grant No. ChineselPM0904） and the National Nature Science Foundation of China （30800107, 30621003, 30525009）. All experiments were done in China according to the rules of the ethical board for animal experiments, complying with the current laws of this country.
文摘We evaluated the attraction ofMonochamus alternatus Hope （Coleoptera： Cerambycidae）, Dryocoetes luteus Blandford and Orthotomicus erosus Wollaston （Coleoptera： Curculionidae： Scolytinae） to multiple-funnel traps baited with the pine volatiles, ethanol and （＋）-α-pinene and the bark beetle pheromones, ipsenol and ipsdienol. M. alternatus were attracted to traps baited with ethanol and （＋）-α-pinene but not those baited with ipsdienol and ipsenol. Ipsdienol and ipsenol decreased catches ofM. alternatus in traps baited with ethanol and （＋）-α-pinene. Traps baited with either binary combinations of ethanol and （＋）-α-pinene or ipsdienol and ipsenol were attractive to D. luteus and O. erosus. The addition of ipsenol and ipsdienol to traps baited with ethanol and （＋）-α-pinene synergized attraction of O. erosus but not D. luteus .
