Lygodium flexuosum extract down regulates the expression of proinflammatory cytokines in CCl_4 -induced hepatotoxicity

Objective:To examine the downregulalion of proinflammatory cytokines in a time dependant manner on carbon tetrachloride induced toxicity in experimental animals.Methods:CCl4（150μL/100 g） was dissolved in com oil（1:1 v/v%） and administered orally,GroupⅠwas treated as normal control and received corn oil on 8th day.GroupⅡwas toxic control and was given a single dose of CCl4 on 8th days.GroupⅢwas treated with Lygodium flexuosum（L.flexuosum） n-hexane extract（200 mg/kg） for 8 days and on 8th day a single dose of CCl4 was received.GroupⅣ（negative contnil） received L.flexuosiim n-hexane extract（200 mg/kg） alone for 8 days.Results:Treatment with n-hexane extract prior to the administration of CCl4 significantly prevented an increase in serum AST,ALT,LDH activity and lipid peroxidation and prevented the depletion of glutathione （GSH）.Rats treated with L.flexuosum had reduced mRNA levels of TGF-β1,TNF-αand IL-1βgenes in liver of CCl4 intoxicated rats when compared to CCl4 control as evidenced by RT-PCR. Conclusions:The data suggest that L.flexuosum,a widely available fem,significantly reduces CCl4 induced acute hepatotoxicily by down-regulating the expression of pro-inflammatory cytokines in rats.

曲轴海金沙与小叶海金沙中总黄酮的含量测定

目的:建立测定曲轴海金沙和小叶海金沙中总黄酮的含量方法。方法:采用紫外-可见分光光度法,在510 nm的波长处测定吸光度,计算出供试品溶液中总黄酮的含量。结果:曲轴海金沙和小叶海金沙在6.0～37 g.L-1(r=0.999 6)范围内吸收值与其浓度呈良好的线性关系。曲轴海金沙总黄酮的含量为10.57%,RSD为0.49%;小叶海金沙为6.58%,RSD为0.72%。结论:采用紫外-可见分光光度法能够对曲轴海金沙和小叶海金沙中总黄酮的含量进行测定,方法快速、灵敏度高、重现性好。