The capability of recombinant human interleukin-2 ( rhIL-2) and lymphokine-activated killer (LAK) cells in the purging of normal human bone marrows contaminated with human myeloid leukemic cell lines was evaluated. Mi...The capability of recombinant human interleukin-2 ( rhIL-2) and lymphokine-activated killer (LAK) cells in the purging of normal human bone marrows contaminated with human myeloid leukemic cell lines was evaluated. Mixtures of normal human bone marrow mononuclear cells ( BMC) and K562 cells or HL-60 cells (at the BMCK562 ratio of 200:1, 100:1 or 20:1) were incubated with IL-2 with or without LAK cells at the BMC:LAK ratio of 1:1 for one or three days. The nubmers of residual K562 cells, BFU-E and CFU-GM were examined by clonogenic assays. In 200:1 mixture groups without LAK cells, the number of K562 colonies reduced by 50% with no loss of BFU-E and CFU-GM in one-day cultures, and no K562 colonies formed in three-day cultures with about 20% loss of BFU-E and CFU-GM. If the BMC.K562 ratios were 100:1 or 20:1 in the mktures, the leukemic cells could not be eliminated. When the mixtures were incubated with IL-2 and LAK cells, no leukemic cell colonies were detected in the 20:1 group following one-day展开更多
Aim. To study the effect of kanglemycin C (KC) on production and gene transcription of lymphokins. Methods.Cell proliferation and lymphokin activities were quantified with MTT colorimetry and ELISA, and gene transcrip...Aim. To study the effect of kanglemycin C (KC) on production and gene transcription of lymphokins. Methods.Cell proliferation and lymphokin activities were quantified with MTT colorimetry and ELISA, and gene transcriptions of lymphokins semi quantified with RT PCR. Results.Suppression of KC on proliferation of enriched T and B cell respectively mediated by Con A and LPS was declined by addition of exogenous IL 1, IL 2, and IL 6. KC 80 nmol/L markedly inhibited IL 2 and IL 6 production and mRNA transcription of incubated mouse splenocytes induced by Con A. Additionally, KC had some suppression on IL 1β and IL 6 productions of peritoneal macrophage stimulated by LPS (5μg/mL), whereas cyclosporine (CS) had not. [WT5”BX]Conclusion. [WT5”BZ]Immunosuppression of KC came true partially through the decrease of IL 1β, 2 and 6 productions, especially of IL 2. However, CS′s immunosuppression was mainly through the decrease of IL 2 procduction.展开更多
文摘The capability of recombinant human interleukin-2 ( rhIL-2) and lymphokine-activated killer (LAK) cells in the purging of normal human bone marrows contaminated with human myeloid leukemic cell lines was evaluated. Mixtures of normal human bone marrow mononuclear cells ( BMC) and K562 cells or HL-60 cells (at the BMCK562 ratio of 200:1, 100:1 or 20:1) were incubated with IL-2 with or without LAK cells at the BMC:LAK ratio of 1:1 for one or three days. The nubmers of residual K562 cells, BFU-E and CFU-GM were examined by clonogenic assays. In 200:1 mixture groups without LAK cells, the number of K562 colonies reduced by 50% with no loss of BFU-E and CFU-GM in one-day cultures, and no K562 colonies formed in three-day cultures with about 20% loss of BFU-E and CFU-GM. If the BMC.K562 ratios were 100:1 or 20:1 in the mktures, the leukemic cells could not be eliminated. When the mixtures were incubated with IL-2 and LAK cells, no leukemic cell colonies were detected in the 20:1 group following one-day
文摘Aim. To study the effect of kanglemycin C (KC) on production and gene transcription of lymphokins. Methods.Cell proliferation and lymphokin activities were quantified with MTT colorimetry and ELISA, and gene transcriptions of lymphokins semi quantified with RT PCR. Results.Suppression of KC on proliferation of enriched T and B cell respectively mediated by Con A and LPS was declined by addition of exogenous IL 1, IL 2, and IL 6. KC 80 nmol/L markedly inhibited IL 2 and IL 6 production and mRNA transcription of incubated mouse splenocytes induced by Con A. Additionally, KC had some suppression on IL 1β and IL 6 productions of peritoneal macrophage stimulated by LPS (5μg/mL), whereas cyclosporine (CS) had not. [WT5”BX]Conclusion. [WT5”BZ]Immunosuppression of KC came true partially through the decrease of IL 1β, 2 and 6 productions, especially of IL 2. However, CS′s immunosuppression was mainly through the decrease of IL 2 procduction.
