Pathogenic African swine fever virus(ASFV)remains a lethal causative agent in the domestic pig industry,which poses a burden on the swine market and causes substantial socioeconomic losses worldwide.Currently,there ar...Pathogenic African swine fever virus(ASFV)remains a lethal causative agent in the domestic pig industry,which poses a burden on the swine market and causes substantial socioeconomic losses worldwide.Currently,there are no commercially efcacious vaccines or specifc treatments available for ASF prevention and control.Unfortunately,little is known about the swine immune response upon ASFV infection.Here,we investigated the host immune response discrepancy induced by the feld moderately virulent strain ASFV HB-2208 among healthy,diseased and asymptomatic pigs.In the peripheral blood of diseased swine,lymphopenia is caused by the massive loss of bystander lymphocytes,such asγδT cells,B cells and CD4^(+)T cells.Conversely,ASFV has a strong tropism for the mononuclear phagocyte system(MPS)and partial dendritic cells(DCs),whose antigen-presenting ability is impeded by the downregulation of CD80 and MHC I.However,no signifcant diference in the number of CD8α^(high) T cells was detected,whereas the frequencies of NK cells,NKT cells,and regulatory T cells(Tregs)were signifcantly increased.Additionally,an in vitro model was established with a coculture of primary pulmonary alveolar macrophages(PAMs)and peripheral blood mononuclear cells(PBMCs),which signifcantly reducedγδT cells,B cells and CD4^(+)T cells and increased Tregs.The diferentiated immune response might aid in enhancing the understanding of ASFV pathogenesis in suids and provide insights into the mechanism of ASFV-induced lymphopenia for further studies.展开更多
To the Editor:Following H7N9infection,the host lymphocyte immunity plays an antiviral role.[l] Furthermore,low frequencies of T-cells correlate with disease severity.[2]Herein,we present a H7N9-infected patient with l...To the Editor:Following H7N9infection,the host lymphocyte immunity plays an antiviral role.[l] Furthermore,low frequencies of T-cells correlate with disease severity.[2]Herein,we present a H7N9-infected patient with life-threatening lymphopenia (only 0.06× 10^9/L in the peripheral blood),which we have barely ever seen before.We also measured the proportidns of T-cell subpopulations in the blood and bronchoalveolar lavage fluid (BALF).展开更多
BACKGROUND: Exertional heatstroke(EHS) is a life-threatening disease without ideal prognostic markers for predicting hospital mortality.METHODS: This is a single-center retrospective study. Clinical data from EHS pati...BACKGROUND: Exertional heatstroke(EHS) is a life-threatening disease without ideal prognostic markers for predicting hospital mortality.METHODS: This is a single-center retrospective study. Clinical data from EHS patients admitted to the Intensive Care Unit(ICU) of the General Hospital of Southern Theatre Command between January 1, 2008, and December 31, 2020, were recorded and analyzed. Univariate and multivariate logistic regression were used to identify the factors for mortality. The prediction model was developed with the prognostic markers, and a nomogram was established.RESULTS: The study ultimately enrolled 156 patients, and 15(9.6%) of patients died before discharge. The lymphocyte count(Lym) and percentage(Lym%) were significantly lower in nonsurvivors(P<0.05). The univariate and multivariate logistic regression analyses indicated that Lym% at the third day of admission(Lym% D3)(OR=0.609, 95%CI: 0.454–0.816) and hematocrit(HCT)(OR=0.908, 95%CI: 0.834–0.988) were independent protective factors for hospital mortality. A nomogram incorporating Lym% D3 with HCT was developed and demonstrated good discrimination and calibration ability. The comparison between the prediction model and scoring systems revealed that the prediction model had the largest area under the curve(AUC)(0.948, 95%CI: 0.900–0.977), with 100.00% sensitivity and 83.69% specificity, and a greater clinical net benefit.CONCLUSION: Severe EHS patients had a higher risk of experiencing prolonged lymphopenia. A nomogram based on Lym% D3 and HCT was developed to facilitate early identification and timely treatment of patients with potentially unfavorable prognoses.展开更多
Objectives:To determine the correlation between lymphocyte count and A-DROP score in COVID-19 patients and their role in predicting poor outcomes.Methods:This retrospective observational single-center study was conduc...Objectives:To determine the correlation between lymphocyte count and A-DROP score in COVID-19 patients and their role in predicting poor outcomes.Methods:This retrospective observational single-center study was conducted in a tertiary care hospital in Vidisha district,India.COVID-19 patients were included in this study,who were admitted to ICU and the COVID Care Centre from August 2020 to October 2020.Demographic profile,clinical characteristics,medical history,A-DROP score,complete blood counts including lymphocyte counts(on admission),the severity of the disease course,and duration of hospitalization were collected.The correlation between lymphopenia and A-DROP score was determined,and their role in predicting poor outcomes was investigated.Results:This study included 220 patients,among which 134 were male,and 86 were female[mean age(48.98±16.98)years,95%CI:46.72-51.23].Lymphocyte count in COVID-19 patients negatively correlated with the A-DROP score(r=-0.67,P<0.001).The area under the ROC curve was 0.892(95%CI:0.80-0.98,P<0.001)for the lymphocyte count,and the area under the ROC curve was 0.93(95%CI:0.84-1.00,P<0.001)for lymphocyte count-A-DROP.Conclusion:Lymphocyte count along with the A-DROP score on admission could be used to predict the severity of COVID-19 pneumonia and unfavorable outcome.展开更多
基金support provided by the National Natural Sciences Foundation of China(Grant Nos.32172873 and 31941005)the National Programme on Key Research Project of China(2021YFD1800105-2)National Natural Sciences Foundation of China,32172873,Min Cui,31941005,Min Cui,National Programme on Key Research Project of China,2021YFD1800105-2,Min Cui。
文摘Pathogenic African swine fever virus(ASFV)remains a lethal causative agent in the domestic pig industry,which poses a burden on the swine market and causes substantial socioeconomic losses worldwide.Currently,there are no commercially efcacious vaccines or specifc treatments available for ASF prevention and control.Unfortunately,little is known about the swine immune response upon ASFV infection.Here,we investigated the host immune response discrepancy induced by the feld moderately virulent strain ASFV HB-2208 among healthy,diseased and asymptomatic pigs.In the peripheral blood of diseased swine,lymphopenia is caused by the massive loss of bystander lymphocytes,such asγδT cells,B cells and CD4^(+)T cells.Conversely,ASFV has a strong tropism for the mononuclear phagocyte system(MPS)and partial dendritic cells(DCs),whose antigen-presenting ability is impeded by the downregulation of CD80 and MHC I.However,no signifcant diference in the number of CD8α^(high) T cells was detected,whereas the frequencies of NK cells,NKT cells,and regulatory T cells(Tregs)were signifcantly increased.Additionally,an in vitro model was established with a coculture of primary pulmonary alveolar macrophages(PAMs)and peripheral blood mononuclear cells(PBMCs),which signifcantly reducedγδT cells,B cells and CD4^(+)T cells and increased Tregs.The diferentiated immune response might aid in enhancing the understanding of ASFV pathogenesis in suids and provide insights into the mechanism of ASFV-induced lymphopenia for further studies.
基金the grants from the National Natural Science Foundation of China (Nos.1470270and 81401629) the National Key Research and Development Program ofChina (No.2016YFC1304300)the Capital Clinical Features Applied Research and Achievement Promotion Project of Beijing, China (No.Z 161100000516116).
文摘To the Editor:Following H7N9infection,the host lymphocyte immunity plays an antiviral role.[l] Furthermore,low frequencies of T-cells correlate with disease severity.[2]Herein,we present a H7N9-infected patient with life-threatening lymphopenia (only 0.06× 10^9/L in the peripheral blood),which we have barely ever seen before.We also measured the proportidns of T-cell subpopulations in the blood and bronchoalveolar lavage fluid (BALF).
基金supported by the Natural Science Foundation of Guangdong Province (2022A1515010353)Science and Technology Projects of Guangzhou (SL2024A03J00951)Military Medical Innovation Project (18CXZ032)。
文摘BACKGROUND: Exertional heatstroke(EHS) is a life-threatening disease without ideal prognostic markers for predicting hospital mortality.METHODS: This is a single-center retrospective study. Clinical data from EHS patients admitted to the Intensive Care Unit(ICU) of the General Hospital of Southern Theatre Command between January 1, 2008, and December 31, 2020, were recorded and analyzed. Univariate and multivariate logistic regression were used to identify the factors for mortality. The prediction model was developed with the prognostic markers, and a nomogram was established.RESULTS: The study ultimately enrolled 156 patients, and 15(9.6%) of patients died before discharge. The lymphocyte count(Lym) and percentage(Lym%) were significantly lower in nonsurvivors(P<0.05). The univariate and multivariate logistic regression analyses indicated that Lym% at the third day of admission(Lym% D3)(OR=0.609, 95%CI: 0.454–0.816) and hematocrit(HCT)(OR=0.908, 95%CI: 0.834–0.988) were independent protective factors for hospital mortality. A nomogram incorporating Lym% D3 with HCT was developed and demonstrated good discrimination and calibration ability. The comparison between the prediction model and scoring systems revealed that the prediction model had the largest area under the curve(AUC)(0.948, 95%CI: 0.900–0.977), with 100.00% sensitivity and 83.69% specificity, and a greater clinical net benefit.CONCLUSION: Severe EHS patients had a higher risk of experiencing prolonged lymphopenia. A nomogram based on Lym% D3 and HCT was developed to facilitate early identification and timely treatment of patients with potentially unfavorable prognoses.
文摘Objectives:To determine the correlation between lymphocyte count and A-DROP score in COVID-19 patients and their role in predicting poor outcomes.Methods:This retrospective observational single-center study was conducted in a tertiary care hospital in Vidisha district,India.COVID-19 patients were included in this study,who were admitted to ICU and the COVID Care Centre from August 2020 to October 2020.Demographic profile,clinical characteristics,medical history,A-DROP score,complete blood counts including lymphocyte counts(on admission),the severity of the disease course,and duration of hospitalization were collected.The correlation between lymphopenia and A-DROP score was determined,and their role in predicting poor outcomes was investigated.Results:This study included 220 patients,among which 134 were male,and 86 were female[mean age(48.98±16.98)years,95%CI:46.72-51.23].Lymphocyte count in COVID-19 patients negatively correlated with the A-DROP score(r=-0.67,P<0.001).The area under the ROC curve was 0.892(95%CI:0.80-0.98,P<0.001)for the lymphocyte count,and the area under the ROC curve was 0.93(95%CI:0.84-1.00,P<0.001)for lymphocyte count-A-DROP.Conclusion:Lymphocyte count along with the A-DROP score on admission could be used to predict the severity of COVID-19 pneumonia and unfavorable outcome.
