The lysozyme crystals were made by batch crystallization method and the distribution of aggregate in solution were measured by dynamic light scattering. The results showed that the dimension of aggregate increased wit...The lysozyme crystals were made by batch crystallization method and the distribution of aggregate in solution were measured by dynamic light scattering. The results showed that the dimension of aggregate increased with the increase of the concentration of lysozyme and NaCl, lysozyme molecules aggregated gradually in solution and finally arrived at balance each other. The higher the concentrations of lysozyme and NaCl were, the faster the growth rate of （110） face was. The growth rates of lysozyme crystal were obtained by a Zeiss microscope, and the effective surface energy （a） of growing steps were calculated about 4.01×10^-8s J·cm^-2 according to the model of multiple two-dimensional nucleation mechanism.展开更多
The interaction between protein and solvent is important in determination of the conformation and stability of natural protein.The first publication of protein structure was based on the thermodynamic rule that hydrop...The interaction between protein and solvent is important in determination of the conformation and stability of natural protein.The first publication of protein structure was based on the thermodynamic rule that hydrophobic amino acids in the展开更多
基金supported by the National Natural Science Foundation of China(No.10472127 and No.10432060)Knowledge Innovation Program of Chinese Academy of Sciences(KSCX2-SW-322,KICX2-SW-L05).
文摘The lysozyme crystals were made by batch crystallization method and the distribution of aggregate in solution were measured by dynamic light scattering. The results showed that the dimension of aggregate increased with the increase of the concentration of lysozyme and NaCl, lysozyme molecules aggregated gradually in solution and finally arrived at balance each other. The higher the concentrations of lysozyme and NaCl were, the faster the growth rate of （110） face was. The growth rates of lysozyme crystal were obtained by a Zeiss microscope, and the effective surface energy （a） of growing steps were calculated about 4.01×10^-8s J·cm^-2 according to the model of multiple two-dimensional nucleation mechanism.
基金Project supported by the Nattonal Natural Science Foundation of China
文摘The interaction between protein and solvent is important in determination of the conformation and stability of natural protein.The first publication of protein structure was based on the thermodynamic rule that hydrophobic amino acids in the
