The cDNA chimeras between two subtypes of mouse excitatory amino acid transporter family, mouse excitatory amino acid carrier 1 （mEAAC1） and mouse alanine serine cysteine transporter 1 （mASCT1）, were constructed b...The cDNA chimeras between two subtypes of mouse excitatory amino acid transporter family, mouse excitatory amino acid carrier 1 （mEAAC1） and mouse alanine serine cysteine transporter 1 （mASCT1）, were constructed by recombinant PCR. After transcription in vitro, the cRNA was injected and expressed in Xenopus laevis oocytes. <sup>3</sup>H-Glu and <sup>3</sup>H-Ser were used as isotopic tracer to measure the flux of amino acids. The results showed that there might not be the key amino acids responsible for substractive specificity in the NH<sub>2</sub>-terminal and its adjacent regions of these two transporters, which probably supported the formation of the substrata binding sites.展开更多
文摘The cDNA chimeras between two subtypes of mouse excitatory amino acid transporter family, mouse excitatory amino acid carrier 1 （mEAAC1） and mouse alanine serine cysteine transporter 1 （mASCT1）, were constructed by recombinant PCR. After transcription in vitro, the cRNA was injected and expressed in Xenopus laevis oocytes. <sup>3</sup>H-Glu and <sup>3</sup>H-Ser were used as isotopic tracer to measure the flux of amino acids. The results showed that there might not be the key amino acids responsible for substractive specificity in the NH<sub>2</sub>-terminal and its adjacent regions of these two transporters, which probably supported the formation of the substrata binding sites.
