Objective To determine the expression of E-prostanoid1 (EP 1) and F-prostanoid (FP) receptor mRNAs in iris-ciliary bodies of the human eye using in situ hybridization.Methods EP 1 and FP receptor mRNAs were detect...Objective To determine the expression of E-prostanoid1 (EP 1) and F-prostanoid (FP) receptor mRNAs in iris-ciliary bodies of the human eye using in situ hybridization.Methods EP 1 and FP receptor mRNAs were detected by riboprobes labeled with digoxigenin on paraffin sections of the iris-ciliary body tissue of the human eye using in situ hybridization. Results EP 1 and FP receptor mRNAs were highly expressed in blood vessels, muscles and the endothelia of the iris. EP 1 receptor hybridization signals were present in all muscle fibers of the ciliary body. Hybridization signal corresponding to FP receptor mRNA transcript was predominantly expressed in the circular muscle and in the collagenous connective tissues of the ciliary body. FP receptor mRNA was not detected in radial and longitudinal muscles. Conclusions EP 1 and FP receptor mRNAs in human ocular tissues appear to be widely localized in the functional sites of the respective receptor agonists. Selective localization of EP 1 and FP receptor mRNAs in the circular muscles and collagenous connective tissues of the ciliary body suggests that EP 1 and FP receptors play an important role in enhancing uveoscleral outflow of aqueous humor.展开更多
Objectives To observe the expression of βig h3 in normal cornea and keratoconus and to elucidate the role of extracellular matrix in keratoconus Methods In situ hybridization was used to detect the expression of...Objectives To observe the expression of βig h3 in normal cornea and keratoconus and to elucidate the role of extracellular matrix in keratoconus Methods In situ hybridization was used to detect the expression of βig h3 in the cornea The cDNA library was screened with human βig h3 cDNA probe to locate βig h3 mRNA in cells Results Expression of βig h3 was found mainly in the stroma of the normal cornea and keratoconus, but decrease depending on the degree of keratopathy In some serious cases, no expression signal was detected The strongest expression was seen at the border of the normal region and keratoconus Conclusions βig h3,the structural component of the extracellular matrix, can affect cell adhensiveness in the development of corneal fibrous interstitial organization During the development of keratoconus, decreasing levels of βig h3 cause the diminution of corneal steadiness, which is related to formation of keratoconus展开更多
文摘Objective To determine the expression of E-prostanoid1 (EP 1) and F-prostanoid (FP) receptor mRNAs in iris-ciliary bodies of the human eye using in situ hybridization.Methods EP 1 and FP receptor mRNAs were detected by riboprobes labeled with digoxigenin on paraffin sections of the iris-ciliary body tissue of the human eye using in situ hybridization. Results EP 1 and FP receptor mRNAs were highly expressed in blood vessels, muscles and the endothelia of the iris. EP 1 receptor hybridization signals were present in all muscle fibers of the ciliary body. Hybridization signal corresponding to FP receptor mRNA transcript was predominantly expressed in the circular muscle and in the collagenous connective tissues of the ciliary body. FP receptor mRNA was not detected in radial and longitudinal muscles. Conclusions EP 1 and FP receptor mRNAs in human ocular tissues appear to be widely localized in the functional sites of the respective receptor agonists. Selective localization of EP 1 and FP receptor mRNAs in the circular muscles and collagenous connective tissues of the ciliary body suggests that EP 1 and FP receptors play an important role in enhancing uveoscleral outflow of aqueous humor.
文摘Objectives To observe the expression of βig h3 in normal cornea and keratoconus and to elucidate the role of extracellular matrix in keratoconus Methods In situ hybridization was used to detect the expression of βig h3 in the cornea The cDNA library was screened with human βig h3 cDNA probe to locate βig h3 mRNA in cells Results Expression of βig h3 was found mainly in the stroma of the normal cornea and keratoconus, but decrease depending on the degree of keratopathy In some serious cases, no expression signal was detected The strongest expression was seen at the border of the normal region and keratoconus Conclusions βig h3,the structural component of the extracellular matrix, can affect cell adhensiveness in the development of corneal fibrous interstitial organization During the development of keratoconus, decreasing levels of βig h3 cause the diminution of corneal steadiness, which is related to formation of keratoconus
