目的使用mRNA探针检测Ⅱ型胶原基因型的变化,酶联免疫吸附法检测血清Ⅱ型胶原抗体浓度,阐明Ⅱ型胶原基因型改变是Legg-perthes病的病因。方法实验选用健康杂种幼犬20只（年龄为2个月,相当于人3.5岁）,雌雄不限,采用自身对照方法,任选一...目的使用mRNA探针检测Ⅱ型胶原基因型的变化,酶联免疫吸附法检测血清Ⅱ型胶原抗体浓度,阐明Ⅱ型胶原基因型改变是Legg-perthes病的病因。方法实验选用健康杂种幼犬20只（年龄为2个月,相当于人3.5岁）,雌雄不限,采用自身对照方法,任选一侧髋为实验侧,另一侧作为对照侧,实验侧股骨颈内注射医用TH胶1 m L制作Legg-Perthes病动物模型,对照侧注入生理盐水1 m L。于注胶后4、8、12周时分批处死取材,采用mRNA探针检测Ⅱ型胶原基因型及酶联免疫吸附法检测血清Ⅱ型胶原抗体浓度。结果Ⅱ型胶原基因型及血清Ⅱ型胶原抗体浓度测定发现在第8~12周时实验组与对照组之间均有极显著性差异（P〈0.01）。结论Ⅱ型胶原基因型的改变可能是Legg-perthes病的病因,可能是因为Ⅱ型胶原刺激产生相应抗体发生特异性免疫反应,导致自身免疫攻击,参与Leggperthe病的发病过程。展开更多
Objective： The aim of this study was to investigate the characteristics of gene changes from Barrett＇s esophagus （BE） to esophageal adenocarcinoma by cDNA microarray. Methods： The cDNA retro-transcribed from equa...Objective： The aim of this study was to investigate the characteristics of gene changes from Barrett＇s esophagus （BE） to esophageal adenocarcinoma by cDNA microarray. Methods： The cDNA retro-transcribed from equal quantity mRNA from esophageal carcinoma and BE tissues as well as control normal epithelium of esophagus which were from one patient with esophageal adenocarcinoma were labeled with Cy5 and Cy3 fluorescence as probes. The mixed probes were hybridized with two pieces gene chip respectively. It was scanned by laser scanner Scan Array 4000. The acquired images were analyzed by software GenePix Pro 3.0. Results： A total of 214 genes were screened out which expression levels were more than 2 times in hybridization of esophageal adenocarcinoma vs normal epithelium of esophagus, whereas 90 genes in hybridization of BE vs normal epithelium. A parallel comparison among these two gene profiles showed that a total of 45 genes with 24 downregulation and 21 up-regulation which expression levels were more than 2 times between the BE and the esophageal adenocarcinoma. Among these, there were 27 genes with 18 downregulafion and 9 up-regulation which implicated the tendencies progressing from BE to esophageal adenocarcinoma. Conclusion： These genes or their products which implicate the tendencies can be chosen as indicators of carcinogenesis with high risk index for BE.展开更多
文摘目的使用mRNA探针检测Ⅱ型胶原基因型的变化,酶联免疫吸附法检测血清Ⅱ型胶原抗体浓度,阐明Ⅱ型胶原基因型改变是Legg-perthes病的病因。方法实验选用健康杂种幼犬20只（年龄为2个月,相当于人3.5岁）,雌雄不限,采用自身对照方法,任选一侧髋为实验侧,另一侧作为对照侧,实验侧股骨颈内注射医用TH胶1 m L制作Legg-Perthes病动物模型,对照侧注入生理盐水1 m L。于注胶后4、8、12周时分批处死取材,采用mRNA探针检测Ⅱ型胶原基因型及酶联免疫吸附法检测血清Ⅱ型胶原抗体浓度。结果Ⅱ型胶原基因型及血清Ⅱ型胶原抗体浓度测定发现在第8~12周时实验组与对照组之间均有极显著性差异（P〈0.01）。结论Ⅱ型胶原基因型的改变可能是Legg-perthes病的病因,可能是因为Ⅱ型胶原刺激产生相应抗体发生特异性免疫反应,导致自身免疫攻击,参与Leggperthe病的发病过程。
文摘Objective： The aim of this study was to investigate the characteristics of gene changes from Barrett＇s esophagus （BE） to esophageal adenocarcinoma by cDNA microarray. Methods： The cDNA retro-transcribed from equal quantity mRNA from esophageal carcinoma and BE tissues as well as control normal epithelium of esophagus which were from one patient with esophageal adenocarcinoma were labeled with Cy5 and Cy3 fluorescence as probes. The mixed probes were hybridized with two pieces gene chip respectively. It was scanned by laser scanner Scan Array 4000. The acquired images were analyzed by software GenePix Pro 3.0. Results： A total of 214 genes were screened out which expression levels were more than 2 times in hybridization of esophageal adenocarcinoma vs normal epithelium of esophagus, whereas 90 genes in hybridization of BE vs normal epithelium. A parallel comparison among these two gene profiles showed that a total of 45 genes with 24 downregulation and 21 up-regulation which expression levels were more than 2 times between the BE and the esophageal adenocarcinoma. Among these, there were 27 genes with 18 downregulafion and 9 up-regulation which implicated the tendencies progressing from BE to esophageal adenocarcinoma. Conclusion： These genes or their products which implicate the tendencies can be chosen as indicators of carcinogenesis with high risk index for BE.
