Insulin-like growth factor 2 mRNA-binding protein 1 promotes cell proliferation via activation of AKT and is directly targeted by microRNA-494 in pancreatic cancer

BACKGROUND Studies have shown that insulin-like growth factor 2 mRNA-binding protein 1(IGF2BP1)plays critical roles in the genesis and development of human cancers.AIM To investigate the clinical significance and role of IGF2BP1 in pancreatic cancer.METHODS Expression levels of IGF2BP1 and microRNA-494(miR-494)were mined based on Gene Expression Omnibus datasets and validated in both clinical samples and cell lines by quantitative real-time polymerase chain reaction and Western blot.The relationship between IGF2BP1 expression and clinicopathological factors of pancreatic cancer patients was analyzed.The effect and mechanism of IGF2BP1 on pancreatic cancer cell proliferation were investigated in vitro and in vivo.Analyses were performed to explore underlying mechanisms of IGF2BP1 upregulation in pancreatic cancer and assays were carried out to verify the posttranscriptional regulation of IGF2BP1 by miR-494.RESULTS We found that IGF2BP1 was upregulated and associated with a poor prognosis in pancreatic cancer patients.We showed that downregulation of IGF2BP1 inhibited pancreatic cancer cell growth in vitro and in vivo via the AKT signaling pathway.Mechanistically,we showed that the frequent upregulation of IGF2BP1 was attributed to the downregulation of miR-494 expression in pancreatic cancer.Furthermore,we discovered that reexpression of miR-494 could partially abrogate the oncogenic role of IGF2BP1.CONCLUSION Our results revealed that upregulated IGF2BP1 promotes the proliferation of pancreatic cancer cells via the AKT signaling pathway and confirmed that the activation of IGF2BP1 is partly due to the silencing of miR-494.

mRNA-specific translational regulation in yeast

The expression of a gene is governed at various levels,from transcriptional to translational level.The translational control is widely used to regulate gene expression,especially when a rapid,local,and selective control over protein synthesis is required.The present review describes instructive examples of translational regulation in yeast,together with regulatory elements within mRNAs.The review also outlines the important contributions of mRNA-binding proteins that act in harmony with several translational elements to generate appropriate translational signals and responses.

Expression and prediction of genes related to IGF2BP3 in gastric cancer

Objective Gastric cancer(GC)is one of the most prevalent cancers worldwide and is associated with high morbidity and mortality rates.The IGF2 mRNA-binding protein(IGF2BP)participates in a variety of cancers.The aim of this study was to analyze the expression of IGF2BP3 and explore the genes related to IGF2BP3 in GC.Methods Bioinformatics software was used to analyze the expression of IGF2BP1,IGF2BP2,and IGF2BP3 in tumors,and the expression of IGF2BPs in the GSE118897 dataset.Immunohistochemistry was performed to detect the protein level of IGF2BP3 in GC samples.cBioPortal was used to query gene alteration of IGF2BP3.LinkedOmics was used to identify genes related to IGF2BP3.Results Sangerbox analysis showed that the expression of all IGF2BP family members was higher in GC.cBioporta analysis showed that gene alteration of IGF2BP3 in stomach adenocarcinoma included mutation and amplificatio.LinkedOmics analysis showed that many genes were correlated with IGF2BP3,such as PLAGL2,GET4,IGF2BP1,HMGA2,CLDN6,HOXC13,SMARCA2,TMEM66,CIRBP,NFIX,SLC25A12,and CYB5D2.Conclusion In this study,we founded that IGF2BP3 was overexpressed in GC.Furthermore,this study identified potential genes related to IGF2BP3 in GC,which should be studied further.

Insertion/deletion variants within the IGF2BP2 gene identified in reported genome-wide selective sweep analysis reveal a correlation with goat litter size

Insulin-like growth factor 2 m RNA-binding protein 2(IGF2 BP2,also called IMP2)plays an essential role in the development and maturation of germ cells and embryos and is a candidate gene for goat litter size,based on a previous genomewide selective sweep analysis.In this study,the m RNA expression level of IGF2 BP2 was found to be significantly higher in a single-lamb group than in a multi-lamb group.Insertions/deletions(indels)within the goat IGF2 BP2 gene,including P4-Ins-13 bp and P5-Del-12 bp,were verified in 918 Shaanbei White Cashmere(SBWC)female goats.The minor allelic frequencies(MAFs)of P4-Ins-13 bp and P5-Del-12 bp loci were 0.349 and 0.295,respectively.Analysis using the Chi-square(χ^(2))test showed that the genotype(χ^(2)=14.479,P=0.006)distribution of P4-Ins-13 bp was significantly different between the single-lamb and multi-lamb groups.Correlation analysis demonstrated that P4-Ins-13 bp was significantly associated with goat litter size(P=0.022),and individual goats with the homozygous deletion/deletion(DD)genotype produced more litters than other goats.Therefore,considered as a potential molecular marker significantly related to lambing traits,the P4-Ins-13 bp mutation of the goat IGF2 BP2 gene can be used in goat breeding with practical molecular marker-assisted selection(MAS)to optimize female reproduction and improve economic efficiency in the goat industry.