Michelia macclurei Dandy is a significant tree species that has extensive cultivation for forestry and horticulture purposes in southern China,owing to its economic and practical importance.Light quality influences pl...Michelia macclurei Dandy is a significant tree species that has extensive cultivation for forestry and horticulture purposes in southern China,owing to its economic and practical importance.Light quality influences plantlet growth and development during tissue culture.However,the growth characteristic and molecular regulation of M.macclurei under different light quality conditions are not well understood yet.In this study,we investigated the morphological,chlorophyll content,and transcriptomic responses of M.macclurei plantlets under different light-emitting diode(LED)qualities,including white,blue,and red light.The results showed that blue light significantly increased plant height(21.29%)and leaf number(18.65%),while red light decreased plant height and leaf number by 7.53%and 16.49%,respectively.In addition,the plantlets’chlorophyll content and etiolation rate were significantly reduced by blue and red light quality compared to white light.Compared to white light,blue light had a negative effect,leading to decreased rooting rate(64.28%),root number(72.72%),and root length(75.86%).Conversely,red light had a positive effect,resulting in increased rooting rate(24.99%),root number(109.58%),and root length(72.72%).Transcriptome analysis identified 54 differentially expressed genes(DEGs)in three groups that consisted of blue light vs.white light(BL-vs-WL),red light vs.white light(RL-vs-WL),and red light vs.blue light(RL-vs-BL).Specifically,21,7,and 41 DEGs were identified in the three groups,respectively.The DEGs found in the RL-vs-WL and BL-vs-WL groups were involved in plant hormone signaling,nitrogen metabolism,and phenylpropanoid biosynthesis pathways,which suggests that M.macclurei plantlets adapt to the changes of light quality via modulating gene expression.Overall,our study provides valuable insights for understanding the molecular and morphological responses of M.macclurei plantlets under different light qualities.展开更多
Tree species composition was important for carbon storage within the same climate range.To quantify the dynamics of ecosystem carbon allocation as affected by different tree species,we measured the above-and below-gro...Tree species composition was important for carbon storage within the same climate range.To quantify the dynamics of ecosystem carbon allocation as affected by different tree species,we measured the above-and below-ground biomass accumulation in 22 years,as well as the tissue carbon concentrations of trees in Cunninghamia lanceolata plantation and Michelia macclurei plantation.Results indicated that M.macclurei plantation significantly stored more carbon（174.8 tons/hm2） than C.lanceolata plantation（154.3 tons/hm2）.Most of the carbon was found in the soil pool（57.1% in M.macclurei plantation,55.2% in C.lanceolata plantation）.Tree and soil component of M.macclurei plantation possessed significantly higher carbon storage than that of C.lanceolata plantation（p 〈 0.05）.No significant difference was found in the carbon storage of understory and forest floor.These results suggest that the broadleaved species（M.macclurei） possesses greater carbon sequestration potential than the coniferous species（C.lanceolata） in southern China.展开更多
基金funded and supported by Fundamental Research Funds of the Chinese Academy of Forestry,No.CAFYBB2017MB10.
文摘Michelia macclurei Dandy is a significant tree species that has extensive cultivation for forestry and horticulture purposes in southern China,owing to its economic and practical importance.Light quality influences plantlet growth and development during tissue culture.However,the growth characteristic and molecular regulation of M.macclurei under different light quality conditions are not well understood yet.In this study,we investigated the morphological,chlorophyll content,and transcriptomic responses of M.macclurei plantlets under different light-emitting diode(LED)qualities,including white,blue,and red light.The results showed that blue light significantly increased plant height(21.29%)and leaf number(18.65%),while red light decreased plant height and leaf number by 7.53%and 16.49%,respectively.In addition,the plantlets’chlorophyll content and etiolation rate were significantly reduced by blue and red light quality compared to white light.Compared to white light,blue light had a negative effect,leading to decreased rooting rate(64.28%),root number(72.72%),and root length(75.86%).Conversely,red light had a positive effect,resulting in increased rooting rate(24.99%),root number(109.58%),and root length(72.72%).Transcriptome analysis identified 54 differentially expressed genes(DEGs)in three groups that consisted of blue light vs.white light(BL-vs-WL),red light vs.white light(RL-vs-WL),and red light vs.blue light(RL-vs-BL).Specifically,21,7,and 41 DEGs were identified in the three groups,respectively.The DEGs found in the RL-vs-WL and BL-vs-WL groups were involved in plant hormone signaling,nitrogen metabolism,and phenylpropanoid biosynthesis pathways,which suggests that M.macclurei plantlets adapt to the changes of light quality via modulating gene expression.Overall,our study provides valuable insights for understanding the molecular and morphological responses of M.macclurei plantlets under different light qualities.
基金supported by the Knowledge Innovation Program of the Chinese Academy of Sciences(No. KZCX2-YW-405)the Innovation Group Project of National Natural Science Foundation of China (No.40621061)
文摘Tree species composition was important for carbon storage within the same climate range.To quantify the dynamics of ecosystem carbon allocation as affected by different tree species,we measured the above-and below-ground biomass accumulation in 22 years,as well as the tissue carbon concentrations of trees in Cunninghamia lanceolata plantation and Michelia macclurei plantation.Results indicated that M.macclurei plantation significantly stored more carbon（174.8 tons/hm2） than C.lanceolata plantation（154.3 tons/hm2）.Most of the carbon was found in the soil pool（57.1% in M.macclurei plantation,55.2% in C.lanceolata plantation）.Tree and soil component of M.macclurei plantation possessed significantly higher carbon storage than that of C.lanceolata plantation（p 〈 0.05）.No significant difference was found in the carbon storage of understory and forest floor.These results suggest that the broadleaved species（M.macclurei） possesses greater carbon sequestration potential than the coniferous species（C.lanceolata） in southern China.
