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Mycoplasma pneumoniae Macrolide Resistance and MLVA Typing in Children in Beijing,China,in 2016:Is It Relevant? 被引量:11
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作者 DOU Hai Wei TIAN Xiu Jun +8 位作者 XIN De Li WEI Ran ZHOU Wei WANG Hong QIN Xuan Guang SHAO Jun Yan XU Bao Ping GE Li Xia SHI Da Wei 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2020年第12期916-924,共9页
Objective The aim of this study is to investigate the macrolide resistance rate and molecular type with multiple-locus variable-number tandem-repeat analysis(MLVA)of Mycoplasma pneumoniae of Beijing in 2016 in pediatr... Objective The aim of this study is to investigate the macrolide resistance rate and molecular type with multiple-locus variable-number tandem-repeat analysis(MLVA)of Mycoplasma pneumoniae of Beijing in 2016 in pediatric patients.Methods Real-time quantitative polymerase chain reaction(PCR)was used to identify M.pneumoniae,and MLVA was performed.The domain V of the 23 S rRNA was sequenced to detect macrolide-resistant point mutations.We also investigated the activities of antibiotics against M.pneumoniae isolates in vitro.Results The PCR detection rate of M.pneumoniae in children in Beijing was 40%,and the macrolide resistance rate was 66%.The A2063 G mutation in the 23 S rRNA V region is the dominant mutation(137/146,93.84%),whereas the A2064 G mutation is rare(9/146,6.16%).Seventy-three samples were typed successfully by MLVA typing,including 86.3%(63/73)were MLVA type 4-5-7-2,and 13.7%(10/73)were MLVA type 3-5-6-2.No other types were found.No strains were resistant to levofloxacin or tetracycline.Conclusion In 2016,a specific decrease in the macrolide resistance rate occurred in Beijing.The detection rate and macrolide resistance rate of outpatients are lower than those of inpatients.The A2063 G mutants M.pneumoniae have high levels of resistance to erythromycin and azithromycin.The primary MLVA type is 4-5-7-2,followed by 3-5-6-2.No other MLVA types were detected.No strains resistant to tetracycline or levofloxacin were found in vitro. 展开更多
关键词 CULTURE macrolide resistance MLVA type Mycoplasma pneumoniae
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Effects of Combined Treatment with Sansanmycin and Macrolides on Pseudomonas aeruginosa and Formation of Biofilm 被引量:3
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作者 YUE LI YUN-YING XIE +4 位作者 RU-XIAN CHEN HONG-ZHANG XU GUO-JI ZHANG JIN-ZHE LI AND XIAO-MIAN LI 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2009年第2期170-177,共8页
Objective To observe the effects of combined treatment with sansanmycin and macrolides on Pseudomonas aeruginosa and formation of biofilm. Methods Micro-dilution method was used to determine the minimal inhibitory con... Objective To observe the effects of combined treatment with sansanmycin and macrolides on Pseudomonas aeruginosa and formation of biofilm. Methods Micro-dilution method was used to determine the minimal inhibitory concentrations (MICs) of sansanmycin, gentamycin, carbenicillin, polymyxin B, roxithromycin, piperacillin, and tazobactam. PAl and PA27853 biofilms were observed under optical microscope after staining and under SEM after treatment with sansanmycin at different dosages and combined treatment with sansanmycin and roxithromycin. Viable bacteria in PAl and PA27853 biofilms were counted after treatment with sansanmycin at different dosages or combined treatment with sansanmycin and roxithromycin. Results The MIC of sansanmycin was lower than that of gentamycin and polymyxin B, but was higher than that of carbenicillin. Roxithromycin enhanced the penetration of sansanmycin to PAl and PA27853 strains through biofilms. PAl and PA27853 biofilms were gradually cleared with the increased dosages of sansanmycin or with the combined sansanmycin and roxithromycin. Conclusion Sub-MIC levels of roxithromycin and sansanmycin substantially inhibit the generation of biofilms and proliferation of bacteria. Therefore, combined antibiotics can be used in treatment of intractable bacterial infection. 展开更多
关键词 Sansanmycin macrolideS Pseudomonas aeruginosa BIOFILM
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Synthesis of derivatives of imidazo[4,5-b]pyridine:Novel sulfur contained side chains for macrolide antibiotics 被引量:2
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作者 Lu Liu Peng Xu Liang Zhou Ping Sheng Lei 《Chinese Chemical Letters》 SCIE CAS CSCD 2008年第1期1-4,共4页
Two series of novel derivatives of imidazo[4,5-b]pyridine were synthesized. These compounds could be used as side chains of semisynthesised ketolide antibiotics. The side chains have free amine group which can attache... Two series of novel derivatives of imidazo[4,5-b]pyridine were synthesized. These compounds could be used as side chains of semisynthesised ketolide antibiotics. The side chains have free amine group which can attached to ketolide core. Macrolides with this kind of side chains will show obvious activities against erythromycin-resistant strains. The structure of the side chains was confirmed by ^1H, ^13C NMR, MS, HMBC spectra. 2007 Ping Sheng Lei. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All rights reserved. 展开更多
关键词 macrolide Heterarylalkyl sulfur contained side chain Imidazo[4 5-b]pyridine Synthesis HMBC
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Synthesis of novel 15-membered macrolide derivatives 被引量:2
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作者 Rui Qing Xian Shu Tao Ma Bo Jiao 《Chinese Chemical Letters》 SCIE CAS CSCD 2008年第4期409-411,共3页
In order to develop new antibiotics effective against resistant bacteria,a series of novel 15-membered macrolide derivatives were designed and synthesized by the modification of hydroxyl groups at C-11,C-12 and C-4" ... In order to develop new antibiotics effective against resistant bacteria,a series of novel 15-membered macrolide derivatives were designed and synthesized by the modification of hydroxyl groups at C-11,C-12 and C-4" positions.Their structures were confirmed by MS,IR,^1H NMR or ^13C NMR. 展开更多
关键词 macrolide Azithromycin derivatives SYNTHESIS 4"-Carbamate derivatives
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Neutral Complex Extraction and Synergistic Extraction of Macrolide Antibiotics 被引量:1
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作者 李洲 秦峰 +2 位作者 鲍浩 谷雪蔷 戴玲妹 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2000年第4期304-309,共6页
Based on the theory of reactive extraction, new solvent systems were developed to replace butylacetate for extraction of macrolide antibiotics (erythromycin, kitasamycin, spiramycin meleumycin etc.). A new neutral com... Based on the theory of reactive extraction, new solvent systems were developed to replace butylacetate for extraction of macrolide antibiotics (erythromycin, kitasamycin, spiramycin meleumycin etc.). A new neutral complex solvent extraction system, fatty alcohol-kerosene (marked by El), was used for extraction of erythromycin, one of the macrolide antibiotics. The extraction equilibrium equation is obtained, and the extraction distribution is as followsD = exp (-36.33×103/RT + 18.77)[B](o)1.1/(1 + 108.07-PH)The effects of several parameters on extraction equilibrium were investigated. Furthermore, a new synergistic extraction system (marked by E2) was developed, in which another solvent was used as synergistic agent to replace the diluent kerosene in the neutral complex extraction system. Based on these new extraction systems, an improved process for extraction of erythromycin was developed, showing remarkable advantages in technology and economics owing to its low solvent consumption of 3kg per billion unit compared with 9—10 for butylacetate. The recovery process of solvent from raffinate may be eliminated. 展开更多
关键词 macrolide antibiotics ERYTHROMYCIN neutral complex extraction synergistic extraction
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Antifungalmycin,an antifungal macrolide from Streptomyces padanus 702 被引量:1
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作者 Yi-Fen WANG Sai-Jin WEI +2 位作者 Zhi-Ping ZHANG Tong-He ZHAN Guo-Quan TU 《Natural Products and Bioprospecting》 CAS 2012年第1期41-45,共5页
Two polyene macrolide antibiotics:antifungalmycin(1)and fungichromin(2)were isolated from the culture mycelia of Streptomyces padanus 702 via bioactivity-guided fractionation using various chromatographic procedures.T... Two polyene macrolide antibiotics:antifungalmycin(1)and fungichromin(2)were isolated from the culture mycelia of Streptomyces padanus 702 via bioactivity-guided fractionation using various chromatographic procedures.Their structures were elucidated on the basis of spectral analysis,and 1 is a new polyene macrolide.Compounds 1 and 2 showed significant inhibition against Gibberella zeae with EC_(50)values of 26.71 and 2.21μg/mL,Fusicoccum sp.(plantain head blight)with EC_(50)values of 23.4 and 3.17μg/mL,Mucor ssp.8894 with EC_(50)values of 28.80 and 2.11μg/mL,Ustilaginoidea virens with EC_(50)values of 26.72 and 0.21μg/mL,respectively.This shows that the microbial secondary metabolites 1 and 2 have the potential to be developed as agricultural fungicides for use against G.zeae,Fusicoccum sp.,Mucor ssp.8894,and U.virens. 展开更多
关键词 antifungalmycin Streptomyces padanus 702 polyene macrolide BIOCONTROL antifungal activity PHYTOPATHOGEN
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Preliminary Study on Drug Susceptibility Profile and Resistance Mechanisms to Macrolides of Clinical Isolates of Non-tuberculous Mycobacteria from China 被引量:4
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作者 LI Fu LI Gui Lian +7 位作者 PANG Hui LIU Hai Can XIAO Tong Yang LI Shuang Jun LUO Qiao JIANG Yi WANG Rui Bai WAN Kang Lin 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2018年第4期290-299,共10页
Objective Macrolide susceptibility and drug resistance mechanisms of clinical non-tuberculous mycobacteria(NTM) isolates were preliminarily investigated for more accurate diagnosis and treatment of the infection in ... Objective Macrolide susceptibility and drug resistance mechanisms of clinical non-tuberculous mycobacteria(NTM) isolates were preliminarily investigated for more accurate diagnosis and treatment of the infection in China. Methods Four macrolides, including clarithromycin(CLAR), azithromycin(AZM), roxithromycin(ROX), and erythromycin(ERY), were used to test the drug susceptibility of 310 clinical NTM isolates from six provinces of China with the broth microdilution method. Two resistance mechanisms, 23 S r RNA and erm, were analyzed with nucleotide sequence analysis. Results Varied effectiveness of macrolides and species-specific resistance patterns were observed. Most Mycobacterium abscessus subsp. massiliense were susceptible and all M. fortuitum were highly resistant to macrolides. All the drugs, except for erythromycin, exhibited excellent activities against slow-growing mycobacteria, and drug resistance rates were below 22.2%. Only four highly resistant strains harbored 2,058/2,059 substitutions on rrl and none of other mutations were related to macrolide resistance. G2191 A and T2221 C on rrl were specific for the M. abscessus complex(MABC). Seven sites, G2140 A, G2210 C, C2217 G, T2238 C, T2322 C, T2404 C, and A2406 G, were specifically carried by M. avium and M. intracellulare. Three sites, A2192 G, T2358 G, and A2636 G, were observed only in M. fortuitum and one site G2152 A was specific for M. gordonae. The genes erm(39) and erm(41) were detected in M. fortuitum and M. abscessus and inducible resistance was observed in relevant sequevar. Conclusion The susceptibility profile of macrolides against NTM was demonstrated. The well-known macrolide resistance mechanisms, 23 S r RNA and erm, failed to account for all resistant NTM isolates, and further studies are warranted to investigate macrolide resistance mechanisms in various NTM species. 展开更多
关键词 Non-tuberculous mycobacteria macrolide Drug resistance
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Selective Association between Cephalosporin, Quinolone, Macrolide, and Penicillin Antibiotic Consumption and Childhood Obesity in Europe 被引量:1
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作者 Gabor Ternak Zs Edel +3 位作者 Zs Feiszt J. Szekeres B. Visegrady I. Wittmann 《Health》 2015年第10期1306-1314,共9页
The accelerated weight gain in productive animals as a result of feeding antibiotic enriched fodder has been well known for decades. The better energy harvest is the result of modified gut microbiota as a consequence ... The accelerated weight gain in productive animals as a result of feeding antibiotic enriched fodder has been well known for decades. The better energy harvest is the result of modified gut microbiota as a consequence of applied antibiotics. Similar mechanisms might result obesity in humans as well. Objectives: Finding associations between global antibiotic consumption of different classes in EU countries and obesity data in adults and children prove that antibiotics might play a significant role in the development of obesity “epidemics” and related illnesses. Methods: Antibiotic consumption data were compared with obesity figures in adults and children in European countries and statistically analyzed for significance. Results: Significant correlation was found between the average yearly consumption of cephalosporins (p = 0.007), quinolones (p = 0.031), macrolides (p = 0.000083) and childhood obesity data, but no significant association was observed with the average penicillin consumption. No association was observed between adult obesity and any of the antibiotic classes studied. Conclusions: Our results support the hypothesis that different types of antibiotics might influence the development of obesity among children, and this finding can serve as a unified explanation for the development of obesity “epidemics”, similarly to the obesity and gut flora alteration-related diseases (type 2 diabetes mellitus, autism, etc.). 展开更多
关键词 Antibiotic Consumption CEPHALOSPORIN QUINOLONE macrolide PENICILLIN CHILDHOOD OBESITY Adult OBESITY
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Synthesis of novel 15-membered macrolide dimers
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作者 Shu Tao Ma Rui Xin Ma +1 位作者 Rui Qing Xian Bo Jiao 《Chinese Chemical Letters》 SCIE CAS CSCD 2009年第8期931-934,共4页
A series of novel dimers of 15-membered macrolides was synthesized and evaluated. The dimers exhibited excellent activity against erythromycin-susceptible S. pneumonia, but did not show any improved activity against e... A series of novel dimers of 15-membered macrolides was synthesized and evaluated. The dimers exhibited excellent activity against erythromycin-susceptible S. pneumonia, but did not show any improved activity against erythromycin-resistant S. pneumoniae encoded by erm gene. ?2009 Shu Tao Ma. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All fights reserved. 展开更多
关键词 macrolide Azithromycin derivatives D/mers SYNTHESIS
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β-Lactam Templated Macrocyclization:Synthesis of 12-Membered Macrolide
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作者 GuoJuanLIANG JinZhongZHANG AnQiCHEN 《Chinese Chemical Letters》 SCIE CAS CSCD 2005年第5期601-603,共3页
A novel macrolactonization method was developed using a chiral β-lactam as the template. This novel method features that the macrocyclization is simultaneously achieved while a TBS protected hydroxy group is deprotec... A novel macrolactonization method was developed using a chiral β-lactam as the template. This novel method features that the macrocyclization is simultaneously achieved while a TBS protected hydroxy group is deprotected. 展开更多
关键词 LACTAM macrolactonization macrolide templated cyclization.
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A Novel Oxidative N-Demethylation of 12-Membered Macrolide with Lead Tetraacetate
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作者 Wei Ge ZHANG Ying Hua JIN +4 位作者 Ping QI Kai BAO Jin Guang LIN Nai Li WANG Xin Shen YAO 《Chinese Chemical Letters》 SCIE CAS CSCD 2006年第1期9-11,共3页
Reaction of 12-membered macrolide 1 with lead tetraacetate in dichloromethane afforded an unexpected, novel oxidative N-demethyl product 3.
关键词 Oxidative N-demethylation 12-membered macrolide lead tetraacetate.
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The non-antibacterial effects and possible clinical applications of macrolide antibiotics
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作者 童师亮 《Journal of Chongqing University》 CAS 2006年第2期115-118,共4页
As one group of the most widely used oral antibacterial drugs, macrolides have been clinically applied for more than 50 years because of their safety and efficacy. Recently, the non-antibacterial applications of macro... As one group of the most widely used oral antibacterial drugs, macrolides have been clinically applied for more than 50 years because of their safety and efficacy. Recently, the non-antibacterial applications of macrolides are frequently reported, such as the therapy of malignant tumor, exudative pleurisy, pulmonary fibrosis, rheumatoid arthritis, bronchiectasis, asthma, gastrointestinal peristalsis disorders, and so on with new pharmacological effects, including reverse of tumor resistance, anti-chemical adherence, anti-fibrosis, and modulations of gastrointestinal motility as well as immune activity. The in-depth research of marcolides’ non-antibiotic actions can not only extend the application fields of macrolides, but provide a new way to treat some refractory diseases. 展开更多
关键词 macrolideS non-antibacterial effects clinical applications
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Study of Serotypes, Susceptibility to Macrolide and Virulence and Resistance Molecular Profiles in Invasive Strains of <i>Streptococcus agalactiae</i>in Two Argentine Provinces
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作者 Margarita Laczeski Marina Novosak +4 位作者 Roxana Cannistraci Giolito Ana Littvik Jorge Paván Viviana Villalba Marta Vergara 《Advances in Microbiology》 2015年第4期230-243,共14页
A study of invasive strains of Streptococcus agalactiae (GBS) from Cordoba and Misiones, Argentina;was conducted to determine serotypes, the susceptibility to macrolides and molecular profiles of virulence and resista... A study of invasive strains of Streptococcus agalactiae (GBS) from Cordoba and Misiones, Argentina;was conducted to determine serotypes, the susceptibility to macrolides and molecular profiles of virulence and resistance. We studied 17 strains, recovered from cerebrospinal fluid, blood and cellulite and, a strain of trophoblastic remnants from Misiones. The serotypes were determined by agglutination with sera and phenotypes of resistance to macrolide-lincosamide-streptogramin B (MLSB), were determined with the double-disk test (D-test). The confirmation was performed by E-test by ERI and CLI respectively that determined the minimum inhibitory concentration (MIC). Results were interpreted as recommended by the Clinical and Laboratory Standards Institute (CLSI) 2013. Resistance genes: ermB, ermTR and mefA and the virulence genes: bac, bca, rib, lmb, hylB, scpB, fbsA, fbsB and cylB were investigated by conventional PCR. Serotype III (50%) and Ia (50%) were detected in Cordoba. One strain showed cMLSB phenotype, confirmed by MIC. The same strains showed a resistance gene ermB. All studied virulence genes were detected in 100% of these strains. In Misiones, serotypes were III (72.7%), Ia (18.2%) and Ib (9.1%). All strains were susceptible to CLI and ERI by D-test, confirmed by MIC. None of the strains showed resistance genes. Virulence genes bca, rib, hylB, lmb, fbsA, fbsB and cylB were detected in 100% of the strains, bac in 81.8% and scpB in 90.9%. Our results are in accordance with international data, associating higher frequency of serotype III of invasive neonatal disease followed by Ia. The presence of serotype Ib could indicate a regional difference for Misiones. We highlight the macrolides susceptibility in strains of Misiones and consistency in the results for D-test, MIC and PCR for the single strain resistant phenotype cMLSB from Cordoba. The virulence genes studied were presented with high frequency as expected for invasive strains. 展开更多
关键词 Streptococcus AGALACTIAE Perinatal Infection macrolideS Resistence GENES VIRULENCE GENES
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Macrolide-Clarithromycin Task-Force for the Treatment and Prophylaxis of Covid-19 as a Single Agent
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作者 Eduardo Mansilla Ricardo Rangel Martínez +6 位作者 Gustavo Horacio Marín Idiberto Zotarelli Filho Elsa Rivas Jaime Rivas Katherine Athayde Teixeira de Carvalho Mohammad Reza Dayer Alí Samadikuchaksaraei 《Pharmacology & Pharmacy》 2020年第6期85-104,共20页
SARS-CoV-2 is a novel RNA coronavirus responsible of a deadly pandemic: the clinical illness COVID-19. With only one authorized drug for emergency use in critically ill patients: Remdesivir, there is not any other app... SARS-CoV-2 is a novel RNA coronavirus responsible of a deadly pandemic: the clinical illness COVID-19. With only one authorized drug for emergency use in critically ill patients: Remdesivir, there is not any other approved drug or vaccine yet with proven potential to overcome this infection. We exposed here many scientific evidences to support our novel idea that a macrolide, basically Clarithromycin, could be effective as a single agent for treatment and prophylaxis of COVID-19. Clarithromycin could change the history of this pandemic. It could reduce the costs of treatment and the potential adverse effects when combining more than one drug such as with Hydroxychloroquine. Clarithromycin treatment and prophylaxis as a single agent could be much more simple, safe and cheaper as giving Chloroquine or Hydroxychloroquine alone or in combination with Azithromycin as well as other therapeutic options. 展开更多
关键词 COVID-19 macrolideS CLARITHROMYCIN Single Agent Therapy PROPHYLAXIS
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Antibiotic Treatment for Chronic Rhinosinusitis after Endoscopic Surgery: How Long Should Macrolide Antibiotics Be Given?
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作者 Motohiro Sawatsubashi Daisuke Murakami Shizuo Komune 《International Journal of Otolaryngology and Head & Neck Surgery》 2015年第1期44-49,共6页
Background: The purpose of this study was to determine an appropriate period for macrolide antibiotic therapy, and to investigate whether this period could be shorter, for patients with chronicrhino sinusitis (CRS) af... Background: The purpose of this study was to determine an appropriate period for macrolide antibiotic therapy, and to investigate whether this period could be shorter, for patients with chronicrhino sinusitis (CRS) after functional endoscopic sinus surgery (FESS). Methods: A retrospective analysis of 41 patients undergoing FESS for CRS was performed. All patients underwent pre-operative computed tomography (CT). Patients with fungal sinusitis, allergic fungal sinusitis, and eosinophilic sinusitis were excluded. After FESS, normalized sinus mucosa was confirmed by CT and endoscopy in all patients. Postoperative antibiotic therapy consisted of first-line and second-line regimens. Garenoxacin (GRNX), or clarithromycin (CAM, 400 mg/day) was used as the first-line regimens and low-dose macrolide therapy (CAM, 200 mg/day) was used as the second-line regimen and was prescribed at outpatient visits based on our clinical criteria. Results: Second-line antibiotic therapy (low-dose CAM) was not necessary in 12 of 41 (29%) patients, while it was prescribed in 29 of 41 (71%). The mean duration of low-dose CAM therapy after FESS was 36 days (range 7 to 122 days;median, 25 days). Patients who received second-line therapy (n = 29) were divided into two groups based on the choice of first-line therapy, a GRNX group (n = 13) and a non-GRNX group (n = 16). Those in the non-GRNX had longer periods of postoperative CAM therapy than those in the GRNX group. Conclusion: GRNX was associated with a shorter duration of low-dose macrolide therapy after FESS, and 29% of patients did not need any low-dose macrolide therapy postoperatively. Therefore, macrolide antibiotics should not be routinely prescribed after FESS. 展开更多
关键词 Chronic RHINOSINUSITIS Functional Endoscopic SINUS Surgery macrolide THERAPY GARENOXACIN POSTOPERATIVE Antibiotic THERAPY
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Partial Synthesis of Macrolide Alkaloid Euonine
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作者 Yuan Chao LI Shan Hao JIANG Xiao Yi WANG (Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, 200031 Department of Environmental Chemistry and Engineering, Beijing Polytechnic University,Beijing, 100022) 《Chinese Chemical Letters》 SCIE CAS CSCD 1998年第10期927-928,共2页
The macrolide alkaloid euonine 1 with pharmacologic activity was prepared from the alkaloid wilforgine 2. Their structures were confirmed by IR, NMR, MS and optical rotation.
关键词 TRIPTERYGIUM macrolide alkaloid wilforgin euonine
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Acatulides A-G,neuroprotective macrolides from Acaulium album H-JQSF
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作者 Zhi-Wu Tong Ting-Ting Wang +9 位作者 Pei Yang Jia-Lin Sun Chen-Peng Zhang Salman Khan Xin-Cun Wang Rui-Hua Jiao Hui-Ming Ge Wen-Ying Zhuang Gang Hu Ren Xiang Tan 《Chinese Chemical Letters》 SCIE CAS CSCD 2024年第3期379-383,共5页
Fungal symbionts co-evolve with hosts and microbial co-inhabitants to acquire an unpredictable potential for producing novel bioactive metabolites,but the knowledge about the topic remains patchy and superficial.Here ... Fungal symbionts co-evolve with hosts and microbial co-inhabitants to acquire an unpredictable potential for producing novel bioactive metabolites,but the knowledge about the topic remains patchy and superficial.Here we present the chemical characterization of acatulides A-G(1-7)as architecturally unprecedented macrolides from the solid-state culture of Acaulium album H-JQSF,an arthropod-associated fungus.The acatulide structures were elucidated by spectroscopic analysis,modified Mosher's method and single-crystal X-ray diffraction.The plausible biosynthetic pathways for compounds 1-4 are proposed.Interestingly,acatulides B-D(2-4)and G(7)were demonstrated to be neuroprotective against the 1-methyl-4-phenylpyridinium(MPP+)-induced damage to SH-SY5Y cells and nematode Caenorhabditis elegans(C.elegans). 展开更多
关键词 Acaulium album macrolideS Acatulides A–G Mosher’s method NEUROPROTECTIVE
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P1 gene of Mycoplasmapneumoniae in clinical isolates collected in Beijing in 2010 and relationship between genotyping and macrolide resistance 被引量:9
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作者 Tian Xiu-jun Dong Yan-qing +4 位作者 Dong Xiao-pei Li Jing-yi Li Dan Jiang Yue Xin De-li 《Chinese Medical Journal》 SCIE CAS CSCD 2013年第20期3944-3948,共5页
Background Mycoplasma pneumoniae is a common pathogen that caused community-acquired pneumonia (CAP). P1 protein served as major adhesion and immunodominant protein in Mycoplasma pneumoniae, but little about P1 gene... Background Mycoplasma pneumoniae is a common pathogen that caused community-acquired pneumonia (CAP). P1 protein served as major adhesion and immunodominant protein in Mycoplasma pneumoniae, but little about P1 gene was learned and the relationship between P1 genotype and macrolide resistance has yet to be explored. 展开更多
关键词 Mycoplasma pneumoniae genotype P1 gene macrolide resistance A GT tri-nucleotide
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Enhancing production of a 24-membered ring macrolide compound by a marine bacterium using response surface methodology 被引量:4
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作者 Hua CHEN Mian-bin WU +3 位作者 Zheng-jie CHEN Ming-lu WANG Jian-ping LIN Li-rong YANG 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2013年第4期346-354,共9页
A 24-membered ring macrolide compound,macrolactin A has potential applications in pharmaceuticals for its anti-infectious and antiviral activity.In this study,macrolactin A was produced by a marine bacterium,which was... A 24-membered ring macrolide compound,macrolactin A has potential applications in pharmaceuticals for its anti-infectious and antiviral activity.In this study,macrolactin A was produced by a marine bacterium,which was identified as Bacillus subtilis by 16S ribosomal RNA(rRNA) sequence analysis.Electrospray ionization mass spectrometry(ESI/MS) and nuclear magnetic resonance(NMR) spectroscopy analyses were used to characterize this compound.To improve the production,response surface methodology(RSM) involving Box-Behnken design(BBD) was employed.Faeces bombycis,the main by-product in sericulture,was used as a nitrogen source in fermentation.The interactions between three significant factors,F.bombycis,soluble starch,and(NH4)2SO4 were investigated.A quadratic model was constructed to fit the production and the factors.Optimum medium composition was obtained by analysis of the model.When cultivated in the optimum medium,the production of macrolactin A was increased to 851 mg/L,2.7 times as compared to the original.This study is also useful to find another way in utilizing F.bombycis. 展开更多
关键词 24-membered ring macrolide Enhancing production Response surface methodology Faeces bombycis Marine bacterium
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高效液相色谱-串联质谱法对猪肉中四种大环内酯类药物残留量的测定 被引量:6
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作者 夏曦 李晓薇 +3 位作者 江海洋 李建成 安保超 丁双阳 《分析测试学报》 CAS CSCD 北大核心 2008年第S1期224-226,230,共4页
A simple and sensitive method was developed for the simultaneous determination of four macrolides in pork using high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS).Homogenized sample was extrac... A simple and sensitive method was developed for the simultaneous determination of four macrolides in pork using high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS).Homogenized sample was extracted with NaH2PO4 buffer and acetonitrile solution,and defatted with n-hexane.Further cleanup was performed on a Sep-Pakt C18 solid-phase extraction cartridge.The compounds were determined by LC-MS/MS operated in positive electrospray ionization mode.The limits of detection(LODs) were 0.5 μg/kg.The average recoveries at three spiked concentration levels of 1.0,5.0,10.0 μg/kg were in the range of 70%-110%,with the intra-day RSD of less than 12.9% and inter-day RSD of less than 13.4%. 展开更多
关键词 high performance liquid chromatography-tandem mass SPECTROMETRY PORK macrolide
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