Objective: To investigate the potential role of macrophage colony-stimulating factor (M-CSF) and macrophage colony-stimulating factor receptor (M-CSF-R) on the growth of human hepatoma cells. Methods: Specimens of dif...Objective: To investigate the potential role of macrophage colony-stimulating factor (M-CSF) and macrophage colony-stimulating factor receptor (M-CSF-R) on the growth of human hepatoma cells. Methods: Specimens of different origin, including tissues of human hepatocellular carcinoma (HCC), human fetal liver (FL) and normal liver (NL), the hepatoma cell lines, as well as the peripheral blood mononuclear cells (PBMC) from patients with HCC or liver metastatic tumor (LMT), were used to detect the expression levels of M-CSF and M-CSF-R by ABC immunohistochemistry staining and reverse transcription polymerase chain reaction methods the expression levels of M-CSF and M-CSF-R. Influence of monoclonal antibody against M-CSF (B5) or M-CSF-R (RE2) on proliferation ability of hepatoma cell linesin vitro was also studied. Results: The results showed that hepatoma tissues produced elevated levels of both M-CSF and M-CSF-R compared with those of fetal liver (P<0.001). The M-CSF/M-CSF-R expression levels of PBMC from hepatoma patients were higher than those of LMT patients (P<0.01,P<0.05) and the normal people (P<0.001). The hepatoma cell lines showed strong positive for M-CSF and M-CSF-R production. Both B5 and RE2 displayed a dose-dependent inhibitory effect on the growth and proliferation of hepatoma cells. Conclusion: The study indicates a co-expression model for M-CSF-R in hepatoma cells, suggesting an involvement of M-CSF/M-CSF-R in growth signaling of those malignant cells. The M-CSF/M-CSF-R seems to function through an autonomy mechanism in human hepatoma.展开更多
Objective: To study the potential role of cellular macrophage colony-stimulating factor (cM-CSF) and cellular macrophage colony-stimulating factor receptor (cM-CSF-R) with breast cancer and hepatoma and search the way...Objective: To study the potential role of cellular macrophage colony-stimulating factor (cM-CSF) and cellular macrophage colony-stimulating factor receptor (cM-CSF-R) with breast cancer and hepatoma and search the way for clinical application. Methods: Frozen surgical specimens from 48 breast cancer patients, including 29 cases of histological grade II and 19 eases of grade III, and 16 hepatoma patients were investigated by Avidin Biotin Complex (ABC) immunohistochemical assay with anti-M-CSF monoclonal antibody (Mab) and anti-M-CSF-R Mab. Pathohistological examination was performed as well. Results: cM-CSF and cM-CSF-R were detected in tested specimens. The expression levels of cM-CSF and cM-CSF-R in grade III group were higher than in grade II group and more higher than control group hyperplasia of breast. Hepatoma tissues also showed higher expression level of cM-CSF and cM-CSF-R than normal adult and fetal liver. Conclusion: Breast cancer and hepatoma tissues presented higher expression levels of cM-CSF and cM-CSF-R than control and expression level might be related with tumor’s process.展开更多
Objective: To explore the effects of nuclear M-CSF on the process of tumorigenesis. Methods: Functional part of M-CSF cDNA was inserted into an eukaryotic expression plasmid pCMV/myc/nuc, which can add three NLS to t...Objective: To explore the effects of nuclear M-CSF on the process of tumorigenesis. Methods: Functional part of M-CSF cDNA was inserted into an eukaryotic expression plasmid pCMV/myc/nuc, which can add three NLS to the C-terminal of the expressed protein and direct the protein into the cell nuclei. The constructed plasmid was transferred into NIH3T3 cells and the cell clones were selected by G-418 selection. Cell clones stable expressing target protein were identified by RT-PCR, ABC immunohistochemistry assayand Western blot. Cell growth kinetics analyses throughgrowth curves, cell doubling time, MTT test and anti-sense oligodeoxynucleotide (ASODN) inhibiting cell growth testwere performed to identify cells proliferation potential.Results: The transfected cells showed elevated proliferation potential over the control cells. Conclusion: Abnormalappearance of M-CSF in nucleus could enhance cellproliferation, which suggests that cytokine isoforms within cell nucleus might play transcription factor-like role.展开更多
目的观察高脂饮食对绝经后雌性SD(sprague-dawiey)大鼠骨微结构、骨组织白介素-6(IL-6)及巨噬细胞集落刺激因子(M-CSF)m RNA的影响,探讨高脂对骨微结构的影响及其可能的发病机制。方法将40只6月龄雌性SD大鼠随机分为假手术+普通饲料饲养...目的观察高脂饮食对绝经后雌性SD(sprague-dawiey)大鼠骨微结构、骨组织白介素-6(IL-6)及巨噬细胞集落刺激因子(M-CSF)m RNA的影响,探讨高脂对骨微结构的影响及其可能的发病机制。方法将40只6月龄雌性SD大鼠随机分为假手术+普通饲料饲养组(SHAM组)、假手术+高脂饲料饲养组(HFD组)、去卵巢+普通饲料组(OVX组)、去卵巢+高脂饲料饲养组(OVX+HFD组),每组10只。6个月后用微计算机断层扫描(Micro-CT)等方法检测大鼠右侧胫骨的骨微结构。用实时荧光定量聚合酶链反应(Realtime RT-PCR)测量大鼠左侧股骨中IL-6和M-CSF m RNA的表达差异。结果 6个月后,OVX组的骨体积(BV/TV)、骨小梁数目(Tb.N)、骨小梁厚度(Tb.Th)低于SHAM组,骨小梁分离度(Tb.Sp)大于SHAM组(P<0.05);OVX组BV/TV、Tb.N均大于OVX+HFD组,Tb.Sp小于OVX+HFD组(P<0.05);SHAM组BV/TV、Tb.N、Tb.Th高于SHAM+HFD组,SHAM+HFD与OVX组相比,除Tb.N外,其BV/TV、Tb.Th及Tb.Sp均无明显差异(P>0.05)。OVX组M-CSF和IL-6 m RNA的表达均高于SHAM组(P<0.05)。SHAM的M-CSF和IL-6 m RNA的表达低于SHAM+HFD组(P<0.05),SHAM+HFD的MCSF和IL-6 m RNA的表达低于OVX组(P<0.05),但OVX组与OVX+HFD组比较,M-CSF和IL-6 m RNA的表达无显著差异(P>0.05)。结论高脂引起的骨微结构的改变有可能是通过提高IL-6、M-CSF m RNA水平,导致破骨细胞活性增强、骨吸收增加引起的。展开更多
文摘Objective: To investigate the potential role of macrophage colony-stimulating factor (M-CSF) and macrophage colony-stimulating factor receptor (M-CSF-R) on the growth of human hepatoma cells. Methods: Specimens of different origin, including tissues of human hepatocellular carcinoma (HCC), human fetal liver (FL) and normal liver (NL), the hepatoma cell lines, as well as the peripheral blood mononuclear cells (PBMC) from patients with HCC or liver metastatic tumor (LMT), were used to detect the expression levels of M-CSF and M-CSF-R by ABC immunohistochemistry staining and reverse transcription polymerase chain reaction methods the expression levels of M-CSF and M-CSF-R. Influence of monoclonal antibody against M-CSF (B5) or M-CSF-R (RE2) on proliferation ability of hepatoma cell linesin vitro was also studied. Results: The results showed that hepatoma tissues produced elevated levels of both M-CSF and M-CSF-R compared with those of fetal liver (P<0.001). The M-CSF/M-CSF-R expression levels of PBMC from hepatoma patients were higher than those of LMT patients (P<0.01,P<0.05) and the normal people (P<0.001). The hepatoma cell lines showed strong positive for M-CSF and M-CSF-R production. Both B5 and RE2 displayed a dose-dependent inhibitory effect on the growth and proliferation of hepatoma cells. Conclusion: The study indicates a co-expression model for M-CSF-R in hepatoma cells, suggesting an involvement of M-CSF/M-CSF-R in growth signaling of those malignant cells. The M-CSF/M-CSF-R seems to function through an autonomy mechanism in human hepatoma.
基金Foundation item: This work was supported by '863' High Technology Grant of China (No. 102-11-01-03).
文摘Objective: To study the potential role of cellular macrophage colony-stimulating factor (cM-CSF) and cellular macrophage colony-stimulating factor receptor (cM-CSF-R) with breast cancer and hepatoma and search the way for clinical application. Methods: Frozen surgical specimens from 48 breast cancer patients, including 29 cases of histological grade II and 19 eases of grade III, and 16 hepatoma patients were investigated by Avidin Biotin Complex (ABC) immunohistochemical assay with anti-M-CSF monoclonal antibody (Mab) and anti-M-CSF-R Mab. Pathohistological examination was performed as well. Results: cM-CSF and cM-CSF-R were detected in tested specimens. The expression levels of cM-CSF and cM-CSF-R in grade III group were higher than in grade II group and more higher than control group hyperplasia of breast. Hepatoma tissues also showed higher expression level of cM-CSF and cM-CSF-R than normal adult and fetal liver. Conclusion: Breast cancer and hepatoma tissues presented higher expression levels of cM-CSF and cM-CSF-R than control and expression level might be related with tumor’s process.
基金This work was supported by a grant from Tianjin Science and Technology Development Project (No. 003119311).
文摘Objective: To explore the effects of nuclear M-CSF on the process of tumorigenesis. Methods: Functional part of M-CSF cDNA was inserted into an eukaryotic expression plasmid pCMV/myc/nuc, which can add three NLS to the C-terminal of the expressed protein and direct the protein into the cell nuclei. The constructed plasmid was transferred into NIH3T3 cells and the cell clones were selected by G-418 selection. Cell clones stable expressing target protein were identified by RT-PCR, ABC immunohistochemistry assayand Western blot. Cell growth kinetics analyses throughgrowth curves, cell doubling time, MTT test and anti-sense oligodeoxynucleotide (ASODN) inhibiting cell growth testwere performed to identify cells proliferation potential.Results: The transfected cells showed elevated proliferation potential over the control cells. Conclusion: Abnormalappearance of M-CSF in nucleus could enhance cellproliferation, which suggests that cytokine isoforms within cell nucleus might play transcription factor-like role.
文摘目的观察高脂饮食对绝经后雌性SD(sprague-dawiey)大鼠骨微结构、骨组织白介素-6(IL-6)及巨噬细胞集落刺激因子(M-CSF)m RNA的影响,探讨高脂对骨微结构的影响及其可能的发病机制。方法将40只6月龄雌性SD大鼠随机分为假手术+普通饲料饲养组(SHAM组)、假手术+高脂饲料饲养组(HFD组)、去卵巢+普通饲料组(OVX组)、去卵巢+高脂饲料饲养组(OVX+HFD组),每组10只。6个月后用微计算机断层扫描(Micro-CT)等方法检测大鼠右侧胫骨的骨微结构。用实时荧光定量聚合酶链反应(Realtime RT-PCR)测量大鼠左侧股骨中IL-6和M-CSF m RNA的表达差异。结果 6个月后,OVX组的骨体积(BV/TV)、骨小梁数目(Tb.N)、骨小梁厚度(Tb.Th)低于SHAM组,骨小梁分离度(Tb.Sp)大于SHAM组(P<0.05);OVX组BV/TV、Tb.N均大于OVX+HFD组,Tb.Sp小于OVX+HFD组(P<0.05);SHAM组BV/TV、Tb.N、Tb.Th高于SHAM+HFD组,SHAM+HFD与OVX组相比,除Tb.N外,其BV/TV、Tb.Th及Tb.Sp均无明显差异(P>0.05)。OVX组M-CSF和IL-6 m RNA的表达均高于SHAM组(P<0.05)。SHAM的M-CSF和IL-6 m RNA的表达低于SHAM+HFD组(P<0.05),SHAM+HFD的MCSF和IL-6 m RNA的表达低于OVX组(P<0.05),但OVX组与OVX+HFD组比较,M-CSF和IL-6 m RNA的表达无显著差异(P>0.05)。结论高脂引起的骨微结构的改变有可能是通过提高IL-6、M-CSF m RNA水平,导致破骨细胞活性增强、骨吸收增加引起的。
