The in vivo effects of Phytolacca acinosa poly-saccharides I (PEP-I) on immunologic cytotoxicity of mouse peritoneal macrophages and its production of tumor necrosis factor (TNF) and interleukin 1 (IL-1) were studied....The in vivo effects of Phytolacca acinosa poly-saccharides I (PEP-I) on immunologic cytotoxicity of mouse peritoneal macrophages and its production of tumor necrosis factor (TNF) and interleukin 1 (IL-1) were studied. PEP-I 80 or 160 mg kg was given ip twice every 4 day. Both doses were found to have significant enhancing activity on macrophages cytotoxicity against S180 sarcoma cells and malignant transformed fibroblast L929 cells. Peritoneal activated macrophages were incubated with LPS for 2 and 24 hrs to induce TNF and IL-1, respectively. The TNF and IL-1 activities were tested from cytotoxicity against L929 cells in an absorbence assay of enzymatic reaction and proliferation of thymocytes co-stimulated assay separately. The optimal time for TNF production was found on day 8. Significant increases in TNF and IL-1 were observed. In comparison of the effect of PEP-I on TNF with that of known priming agent BCG, there was no difference between them, but PEP-I had a high effect on IL-1. These results suggest that cytotoxicity of macrophages primed by PEP-I is closely related to its TNF and IL-1 production.展开更多
We investigated whether Nd_2O_3 treatment results in cytotoxicity and other underlying effects in rat NR8383 alveolar macrophages.Cell viability assessed by the MTT assay revealed that Nd_2O_3 was toxic in a dose-depe...We investigated whether Nd_2O_3 treatment results in cytotoxicity and other underlying effects in rat NR8383 alveolar macrophages.Cell viability assessed by the MTT assay revealed that Nd_2O_3 was toxic in a dose-dependent manner, but not in a time-dependent manner. An ELISA analysis indicated that exposure to Nd203 caused cell damage and enhanced synthesis and release of inflammatory chemokines. A Western blot analysis showed that protein expression levels of caspase-3, nuclear factor-KB （NF-KB） and its inhibitor IKB increased significantly in response to Nd203 treatment. Both NF-KB and caspase-3 signaling were activated, suggesting that both pathways are involved in Nd203 cytotoxicity.展开更多
文摘The in vivo effects of Phytolacca acinosa poly-saccharides I (PEP-I) on immunologic cytotoxicity of mouse peritoneal macrophages and its production of tumor necrosis factor (TNF) and interleukin 1 (IL-1) were studied. PEP-I 80 or 160 mg kg was given ip twice every 4 day. Both doses were found to have significant enhancing activity on macrophages cytotoxicity against S180 sarcoma cells and malignant transformed fibroblast L929 cells. Peritoneal activated macrophages were incubated with LPS for 2 and 24 hrs to induce TNF and IL-1, respectively. The TNF and IL-1 activities were tested from cytotoxicity against L929 cells in an absorbence assay of enzymatic reaction and proliferation of thymocytes co-stimulated assay separately. The optimal time for TNF production was found on day 8. Significant increases in TNF and IL-1 were observed. In comparison of the effect of PEP-I on TNF with that of known priming agent BCG, there was no difference between them, but PEP-I had a high effect on IL-1. These results suggest that cytotoxicity of macrophages primed by PEP-I is closely related to its TNF and IL-1 production.
基金supported by the National Natural Science Foundation of China[No.81660532,81260426]the Natural Science Foundation of Inner Mongolia[No.2016MS(LH)0822]+2 种基金the Science and Technology Plan Project in Inner Mongolia[No.201502080]the Doctoral Scientific Research Foundation of Baotou Medical College[BSJJ201621]the Scientific Research Foundation of Baotou Medical College[BYJJ-YF201613]
文摘We investigated whether Nd_2O_3 treatment results in cytotoxicity and other underlying effects in rat NR8383 alveolar macrophages.Cell viability assessed by the MTT assay revealed that Nd_2O_3 was toxic in a dose-dependent manner, but not in a time-dependent manner. An ELISA analysis indicated that exposure to Nd203 caused cell damage and enhanced synthesis and release of inflammatory chemokines. A Western blot analysis showed that protein expression levels of caspase-3, nuclear factor-KB （NF-KB） and its inhibitor IKB increased significantly in response to Nd203 treatment. Both NF-KB and caspase-3 signaling were activated, suggesting that both pathways are involved in Nd203 cytotoxicity.
