Enrichment of trace bioactive constituents and metabolites from complex biological samples is challenging.This study presented a one-pot synthesis of magnetic polydopamine nanoparticles(Fe3O4@-SiO2@PDA NPs)with multip...Enrichment of trace bioactive constituents and metabolites from complex biological samples is challenging.This study presented a one-pot synthesis of magnetic polydopamine nanoparticles(Fe3O4@-SiO2@PDA NPs)with multiple recognition sites for the magnetic dispersive solid-phase extraction(MDSPE)of ginsenosides from rat plasma treated with white ginseng.The extracted ginsenosides were characterized by combining an ultra-high-performance liquid chromatography coupled to a highresolution mass spectrometry with supplemental UNIFI libraries.Response surface methodology was statistically used to optimize the extraction procedure of the ginsenosides.The reusability of Fe3O4@-SiO2@PDA NPs was also examined and the results showed that the recovery rate exceeded 80%after recycling 6 times.Furthermore,the proposed method showed greater enrichment efficiency and could rapidly determine and characterize 23 ginsenoside prototypes and metabolites from plasma.In comparison,conventional methanol method can only detect 8 ginsenosides from the same plasma samples.The proposed approach can provide methodological reference for the trace determination and characterization of different bioactive ingredients and metabolites of traditional Chinese medicines and food.展开更多
In view of widespread existence and toxicity,removal and detection of bisphenols is imperative to asses environmental risks and reduce harm to human health.Although many techniques have been reported constructing fast...In view of widespread existence and toxicity,removal and detection of bisphenols is imperative to asses environmental risks and reduce harm to human health.Although many techniques have been reported constructing fast and sensitive method remains a challenge.Herein,porous poly(divinylbenzene)polyme was synthesized in-situ on the Fe_(3)O_(4)particles by means of distillation-precipitation polymerization and functioned as sorbents to extract bisphenols.Employing Fe_(3)O_(4)@poly(divinylbenzene)as sorbent,a mag netic solid-phase extraction coupling with liquid chromatography was developed to detect trace bisphe nols in water.This method presented low detection limits(0.01–0.03 ng/m L),high enrichment ability(en richment factor,327–343),and good reproducibility.Moreover,the method showed satisfactory recoverie in the detection of lake water(80.60%-116.2%)and egg sample(75.17%-120.0%).Impressively,Fe_(3)O_(4)@PDVB has excellent adsorption capacity,which can realize rapid kinetic adsorption of bisphenols with equi librium time all less than 10 s.The maximum adsorption capacities reached 1074.8,1049.7,1299.1 and 1329.5 mg/g for bisphenol F,bisphenol A,bisphenol B and bisphenol AF with Langmuir isotherm model The adsorption mechanism of Fe_(3)O_(4)@PDVB to bisphenols was investigated and demonstrated that hy drophobic interactions played a key role,together with assistance ofπ-πstacking interactions and hy drogen interactions.Overall,this work provides a promising sorbent material with ultra-fast and large adsorption capacities for extraction of bisphenols from water.展开更多
基金This work was supported by grants from the National Natural Science Foundation of China Key Program(NO.81530094)General Program(NO.81573574,81873193)the Science and Technology Development Project of Jilin Province(20190201283JC).
文摘Enrichment of trace bioactive constituents and metabolites from complex biological samples is challenging.This study presented a one-pot synthesis of magnetic polydopamine nanoparticles(Fe3O4@-SiO2@PDA NPs)with multiple recognition sites for the magnetic dispersive solid-phase extraction(MDSPE)of ginsenosides from rat plasma treated with white ginseng.The extracted ginsenosides were characterized by combining an ultra-high-performance liquid chromatography coupled to a highresolution mass spectrometry with supplemental UNIFI libraries.Response surface methodology was statistically used to optimize the extraction procedure of the ginsenosides.The reusability of Fe3O4@-SiO2@PDA NPs was also examined and the results showed that the recovery rate exceeded 80%after recycling 6 times.Furthermore,the proposed method showed greater enrichment efficiency and could rapidly determine and characterize 23 ginsenoside prototypes and metabolites from plasma.In comparison,conventional methanol method can only detect 8 ginsenosides from the same plasma samples.The proposed approach can provide methodological reference for the trace determination and characterization of different bioactive ingredients and metabolites of traditional Chinese medicines and food.
基金sponsored by the National Natural Science Foundation of China(Nos.22076038 and 22376053)Henan key scientific research programs to Universities and Colleges(No.22ZX003)。
文摘In view of widespread existence and toxicity,removal and detection of bisphenols is imperative to asses environmental risks and reduce harm to human health.Although many techniques have been reported constructing fast and sensitive method remains a challenge.Herein,porous poly(divinylbenzene)polyme was synthesized in-situ on the Fe_(3)O_(4)particles by means of distillation-precipitation polymerization and functioned as sorbents to extract bisphenols.Employing Fe_(3)O_(4)@poly(divinylbenzene)as sorbent,a mag netic solid-phase extraction coupling with liquid chromatography was developed to detect trace bisphe nols in water.This method presented low detection limits(0.01–0.03 ng/m L),high enrichment ability(en richment factor,327–343),and good reproducibility.Moreover,the method showed satisfactory recoverie in the detection of lake water(80.60%-116.2%)and egg sample(75.17%-120.0%).Impressively,Fe_(3)O_(4)@PDVB has excellent adsorption capacity,which can realize rapid kinetic adsorption of bisphenols with equi librium time all less than 10 s.The maximum adsorption capacities reached 1074.8,1049.7,1299.1 and 1329.5 mg/g for bisphenol F,bisphenol A,bisphenol B and bisphenol AF with Langmuir isotherm model The adsorption mechanism of Fe_(3)O_(4)@PDVB to bisphenols was investigated and demonstrated that hy drophobic interactions played a key role,together with assistance ofπ-πstacking interactions and hy drogen interactions.Overall,this work provides a promising sorbent material with ultra-fast and large adsorption capacities for extraction of bisphenols from water.
