Nuclear magnetic resonance (NMR) spectros-copy has become an important tool in modern biological research. NMR spectra image analysis can be used to analyze the kinetics of biomacromolecular conformational changes. Th...Nuclear magnetic resonance (NMR) spectros-copy has become an important tool in modern biological research. NMR spectra image analysis can be used to analyze the kinetics of biomacromolecular conformational changes. The relationship between the image parameters and the protein dynamics was investigated by using a small globular protein ω-conotoxin SO3 (ω-CTX SO3). The physical meanings of the image parameters were characterized from the results. Comparison of the data from the traditional integral area of specific resonance peaks method and the NMR image analysis method showed the advantages of using NMR spectra image analysis for kinetic analysis of two-state processes monitored by 1D proton NMR.展开更多
Dr. Feng Chen is a chief medical doctor and the vice chairman of the Department of Radiology in Zhong Da Hospital at Southeast University, Nanjing, China and a senior researcher in the Department of Radiology at the C...Dr. Feng Chen is a chief medical doctor and the vice chairman of the Department of Radiology in Zhong Da Hospital at Southeast University, Nanjing, China and a senior researcher in the Department of Radiology at the Catholic University of Leuven, Belgium. His main areas of interest are translational imaging research including stroke, tumor angiogenesis, assessment of therapeutic response in solid tumors, and magnetic resonance contrast media. Dr. Feng Chen has published 44 scientific papers in peer-reviewed international journals. He and his colleagues have developed an imaging platform which includes animal models, animal preparations and multiparametric magnetic resonance imaging (MRI) protocols for translational animal imaging research using clinical machines. His MRI findings on rodent stroke are considered to "serve as a model for future laboratory investigations of treatment of acutestroke and unify the approaches developed for clinical studies". He and his colleagues have introduced a novel liver tumor model in rodents, in which a series of studies concerning the antitumor activity of vascular disrupting agents have been successively conducted and assessed by in vivo MRI, especially by diffusion weighted imaging as an imaging biomarker. His goal is to provide valuable references for clinical practice and to contribute to the translation of animal imaging research into patient applications.展开更多
目的采用基于低频振幅(amplitude of low frequency fluctuation,ALFF)算法的静息态功能磁共振成像(function-al magnetic resonance imaging,fMRI)技术探讨轻度认知功能障碍(mild cognitive impairment,MCI)的客观诊断指标及疏肝法改...目的采用基于低频振幅(amplitude of low frequency fluctuation,ALFF)算法的静息态功能磁共振成像(function-al magnetic resonance imaging,fMRI)技术探讨轻度认知功能障碍(mild cognitive impairment,MCI)的客观诊断指标及疏肝法改善肝郁型MCI患者认知功能的机制。方法以肝郁型MCI患者10例为病例组,年龄、性别、文化程度与之匹配的正常人10例被视为对照组,采集病例组用药前后及对照组静息态fMRI的血氧水平依赖(blood oxygenation level dependent,BOLD)信号,分析ALFF值的差异。结果与对照组相比,病例组ALFF值降低的区域主要位于左侧枕中回和岛盖部额下回(P<0.01,AlphaSim校正),ALFF值增高的区域为右内侧前额叶(P<0.01,AlphaSim校正)。与用药前相比,病例组用药后ALFF值降低的区域主要位于右侧枕中回(P<0.01,AlphaSim校正),ALFF值增高的区域主要集中在左侧颞上回和左侧颞中回(P<0.01,AlphaSim校正)。结论ALFF值有望成为诊断MCI的客观指标,左侧颞上回和左侧颞中回兴奋性增高是疏肝法改善肝郁型MCI患者认知功能的可能机制。展开更多
基金This work was supported by the National Key Basic Research Special Funds of China (Grant No. G1999075607)the 985 Funds of Tsinghua University and the THSJZ of Tsinghua University, China.
文摘Nuclear magnetic resonance (NMR) spectros-copy has become an important tool in modern biological research. NMR spectra image analysis can be used to analyze the kinetics of biomacromolecular conformational changes. The relationship between the image parameters and the protein dynamics was investigated by using a small globular protein ω-conotoxin SO3 (ω-CTX SO3). The physical meanings of the image parameters were characterized from the results. Comparison of the data from the traditional integral area of specific resonance peaks method and the NMR image analysis method showed the advantages of using NMR spectra image analysis for kinetic analysis of two-state processes monitored by 1D proton NMR.
基金Supported by Health Bureau of Jiangsu Province, China Chinese Scholarship Council+2 种基金National Natural Science Foundation of ChinaEuropean Congress of Radiology 2000, EAR-ECR Research and Education Fund Fellowship Grant European Union Asia-Link Project
文摘Dr. Feng Chen is a chief medical doctor and the vice chairman of the Department of Radiology in Zhong Da Hospital at Southeast University, Nanjing, China and a senior researcher in the Department of Radiology at the Catholic University of Leuven, Belgium. His main areas of interest are translational imaging research including stroke, tumor angiogenesis, assessment of therapeutic response in solid tumors, and magnetic resonance contrast media. Dr. Feng Chen has published 44 scientific papers in peer-reviewed international journals. He and his colleagues have developed an imaging platform which includes animal models, animal preparations and multiparametric magnetic resonance imaging (MRI) protocols for translational animal imaging research using clinical machines. His MRI findings on rodent stroke are considered to "serve as a model for future laboratory investigations of treatment of acutestroke and unify the approaches developed for clinical studies". He and his colleagues have introduced a novel liver tumor model in rodents, in which a series of studies concerning the antitumor activity of vascular disrupting agents have been successively conducted and assessed by in vivo MRI, especially by diffusion weighted imaging as an imaging biomarker. His goal is to provide valuable references for clinical practice and to contribute to the translation of animal imaging research into patient applications.
文摘目的采用基于低频振幅(amplitude of low frequency fluctuation,ALFF)算法的静息态功能磁共振成像(function-al magnetic resonance imaging,fMRI)技术探讨轻度认知功能障碍(mild cognitive impairment,MCI)的客观诊断指标及疏肝法改善肝郁型MCI患者认知功能的机制。方法以肝郁型MCI患者10例为病例组,年龄、性别、文化程度与之匹配的正常人10例被视为对照组,采集病例组用药前后及对照组静息态fMRI的血氧水平依赖(blood oxygenation level dependent,BOLD)信号,分析ALFF值的差异。结果与对照组相比,病例组ALFF值降低的区域主要位于左侧枕中回和岛盖部额下回(P<0.01,AlphaSim校正),ALFF值增高的区域为右内侧前额叶(P<0.01,AlphaSim校正)。与用药前相比,病例组用药后ALFF值降低的区域主要位于右侧枕中回(P<0.01,AlphaSim校正),ALFF值增高的区域主要集中在左侧颞上回和左侧颞中回(P<0.01,AlphaSim校正)。结论ALFF值有望成为诊断MCI的客观指标,左侧颞上回和左侧颞中回兴奋性增高是疏肝法改善肝郁型MCI患者认知功能的可能机制。
