Microglia are the resident macrophages of the brain,originally described by Pio del Rio-Hortega(a student of Santiago Ramon y Cajal)in a series of studies in 1919.Since those pioneering studies,many others have follow...Microglia are the resident macrophages of the brain,originally described by Pio del Rio-Hortega(a student of Santiago Ramon y Cajal)in a series of studies in 1919.Since those pioneering studies,many others have followed to describe microglia as complex and multitasking cells with many diverse roles under physiological(e.g.their key role in synapse pruning during development)or diseased conditions.Microglia exist as sentinels or surveyors of the environment that surround neurons,becoming reactive upon a wide array of stimuli and consequently developing an appropriate inflammatory response.In fact,a neuroinflammatory response driven by microglia is found in virtually every disease process that occurs within the central nervous system.Therefore,a better understanding of the mechanisms governing the microglia response is the key to improving the outcome of neurodegenerative conditions.展开更多
Using magnetic nanoparticles to enhance gene transfection,a recently developed technique termed magnetofection,has been shown to be a powerful technology in gene delivery.The most widely used magnetic nanoparticles in...Using magnetic nanoparticles to enhance gene transfection,a recently developed technique termed magnetofection,has been shown to be a powerful technology in gene delivery.The most widely used magnetic nanoparticles in this area are those coated with polyethyleneimine,which is a well known non-viral transfection agent.In this article,we report methods to control the aggregate size of polyethyleneimine-coated magnetite particles.These particles were then used to enhance transfection of green fluorescent protein(GFP)into NIH 3T3 cells in vitro.We find that the aggregate size of the particles has a great effect on their performance in magnetofection,with less aggregated magnetic particles being more effective in enhancing the gene transfection.展开更多
文摘Microglia are the resident macrophages of the brain,originally described by Pio del Rio-Hortega(a student of Santiago Ramon y Cajal)in a series of studies in 1919.Since those pioneering studies,many others have followed to describe microglia as complex and multitasking cells with many diverse roles under physiological(e.g.their key role in synapse pruning during development)or diseased conditions.Microglia exist as sentinels or surveyors of the environment that surround neurons,becoming reactive upon a wide array of stimuli and consequently developing an appropriate inflammatory response.In fact,a neuroinflammatory response driven by microglia is found in virtually every disease process that occurs within the central nervous system.Therefore,a better understanding of the mechanisms governing the microglia response is the key to improving the outcome of neurodegenerative conditions.
基金This work was supported by National 973 Program(2006CBON0300)International Cooperation Project(20080068,075207012).
文摘Using magnetic nanoparticles to enhance gene transfection,a recently developed technique termed magnetofection,has been shown to be a powerful technology in gene delivery.The most widely used magnetic nanoparticles in this area are those coated with polyethyleneimine,which is a well known non-viral transfection agent.In this article,we report methods to control the aggregate size of polyethyleneimine-coated magnetite particles.These particles were then used to enhance transfection of green fluorescent protein(GFP)into NIH 3T3 cells in vitro.We find that the aggregate size of the particles has a great effect on their performance in magnetofection,with less aggregated magnetic particles being more effective in enhancing the gene transfection.
