Simultaneous Determination of Six Components in Cortex Phellodendri by HPLC

[Objectives]This study was conducted to establish the method for simultaneous determination of six active components.[Methods]Simultaneous determination of chlorogenic acid,phellodendrine,magnoflorine,jatrorrhizine,palmatine and berberine in Cortex Phellodendri was carried out by HPLC with a Diamonsil C18(4.6 mm×250 mm,5μm)column was used.The mobile phase was acetonitrile-water(1‰acetic acid,2 mmol ammonium acetate)solution in gradient elution.The detection wavelength was set at 280 nm,and the column temperature was kept at 25℃and the flow rate was 1 ml/min.[Results]The linear ranges of chlorogenic acid,phellodendrine,magnoflorine,jatrorrhizine,palmatine and berberine were 20.00-320.00,18.75-130.00,25.00-200.00,5.00-100.00,20.00-200.00,and 0.09-1.80 mg/L,respectively.The average recovery was 98.1%,99.4%,97.5%,97.3%,104.0%,and 98.5%,respectively;and the RSDs were 0.5%,0.6%,0.8%,1.0%,1.4%,and 0.9%,respectively.[Conclusions]The method is convenient,stable,reliable and suitable for quality control of Cortex Phellodendri.

HPLC测定市售青风藤中青藤碱和木兰花碱含量

目的:建立青风藤中青藤碱和木兰花碱的含量测定方法,并用于评价市售青风藤的质量。方法:采用高效液相色谱法,色谱柱为Synergi Polar-RP苯基C18柱(250 mm×4. 6 mm,4"m),流动相为乙腈(B)-1%乙酸水溶液(A),梯度洗脱(0～15 min,10%→15%B; 15～17 min,15%→20%B; 17～22 min,20%→40%B; 22～30 min,40%→40%B),柱温为30℃;检测波长为280 nm,流速为1 m L·min-1。结果:本方法的精密度、准确度和重现性的RSD皆小于2%,青藤碱和木兰花碱在0. 01～0. 40 mg·m L-1线性关系良好;在测定的62批样品中,除了4批伪品之外,只有1批样品中青藤碱含量低于《中华人民共和国药典》要求,青藤碱和木兰花碱的含量分别为0. 236%～4. 735%和0. 256%～3. 682%。结论:本方法精密度、准确度和重现性良好,适用于青风藤中青藤碱和木兰花碱的含量测定;大多数市售青风藤的质量符合《中华人民共和国药典》要求,但主要成分青藤碱和木兰花碱的含量存在差异。