Around 100 proteins are produced inside the chloroplast but the vast majority must be imported. These proteins are synthesized in the cytosol as preproteins with cleavable targeting sequences. Several plastid types ot...Around 100 proteins are produced inside the chloroplast but the vast majority must be imported. These proteins are synthesized in the cytosol as preproteins with cleavable targeting sequences. Several plastid types other than chloroplasts exist. During plant development, one plastid type may be converted to another, a process referred to as plastid transition (Jarvis and Lopez-Juez, 2013). During plastid transitions, proteome remodeling occurs, and one set of imported proteins is replaced by another that is functionally adapted (Kleffmann et al., 2007). Moreover, plastid proteomes are known to be regulated in an environmentdependent fashion, and chloroplasts may respond to changing light conditions by regulating the composition and abundance of photosynthetic complexes.展开更多
Objective: To directionally clone the ompl gene fromChlamydia trachomatis (Ct) F genotype onto a plasmid vectorfor constructing a rudimentary DNA vaccine. Methods: The complete ompl gene from genomic DNA of CtF genoty...Objective: To directionally clone the ompl gene fromChlamydia trachomatis (Ct) F genotype onto a plasmid vectorfor constructing a rudimentary DNA vaccine. Methods: The complete ompl gene from genomic DNA of CtF genotype wild species was amplified with primers designedby computer. The recombinant gene was obtained byrestriction enzyme cutting, linking the gene with the plasmidvector in vitro, transforming the recombinant gene intobacteria, and extracting the DNA from the bacteria. Results: DNA extracted from the bacteria was composed ofthe ompl gene and plasmid, which is identified by threemethods of singular restrictive enzyme cutting, doublerestrictive enzyme cutting and PCR. Conclusion: Cloning of the ompl gene from the Ct Fgenotype means that a rudimentary DNA vaccine wassuccessfully constructed.展开更多
革兰氏阴性细菌的外膜由脂多糖、磷脂、外膜蛋白和脂蛋白等成分组成,是细菌抵御外界有害物质的首要物理屏障,与细菌致病性和耐药性密切相关.外膜各组分依赖特定的系统进行跨膜转运,包括脂多糖转运系统(lipopolysaccharide transport,Lpt...革兰氏阴性细菌的外膜由脂多糖、磷脂、外膜蛋白和脂蛋白等成分组成,是细菌抵御外界有害物质的首要物理屏障,与细菌致病性和耐药性密切相关.外膜各组分依赖特定的系统进行跨膜转运,包括脂多糖转运系统(lipopolysaccharide transport,Lpt)、脂质不对称维持系统(maintenance of lipid asymmetry,Mla)、β-桶状装配机器(β-barrel assembly machinery,Bam)以及脂蛋白定位系统(localization of lipoprotein,Lol).这些系统能够保证细菌外膜的完整与稳定,被视为维持细菌生命活动的"命门".因此,本文系统地综述革兰氏阴性细菌外膜主要成分的跨膜转运系统结构与功能,并对其未来研究方向进行展望,为新型靶向抗菌类药物研发提供新的思路.展开更多
基金the Swiss National Science Foundation (31003A_156998 and 31003A_176191).
文摘Around 100 proteins are produced inside the chloroplast but the vast majority must be imported. These proteins are synthesized in the cytosol as preproteins with cleavable targeting sequences. Several plastid types other than chloroplasts exist. During plant development, one plastid type may be converted to another, a process referred to as plastid transition (Jarvis and Lopez-Juez, 2013). During plastid transitions, proteome remodeling occurs, and one set of imported proteins is replaced by another that is functionally adapted (Kleffmann et al., 2007). Moreover, plastid proteomes are known to be regulated in an environmentdependent fashion, and chloroplasts may respond to changing light conditions by regulating the composition and abundance of photosynthetic complexes.
文摘Objective: To directionally clone the ompl gene fromChlamydia trachomatis (Ct) F genotype onto a plasmid vectorfor constructing a rudimentary DNA vaccine. Methods: The complete ompl gene from genomic DNA of CtF genotype wild species was amplified with primers designedby computer. The recombinant gene was obtained byrestriction enzyme cutting, linking the gene with the plasmidvector in vitro, transforming the recombinant gene intobacteria, and extracting the DNA from the bacteria. Results: DNA extracted from the bacteria was composed ofthe ompl gene and plasmid, which is identified by threemethods of singular restrictive enzyme cutting, doublerestrictive enzyme cutting and PCR. Conclusion: Cloning of the ompl gene from the Ct Fgenotype means that a rudimentary DNA vaccine wassuccessfully constructed.
文摘革兰氏阴性细菌的外膜由脂多糖、磷脂、外膜蛋白和脂蛋白等成分组成,是细菌抵御外界有害物质的首要物理屏障,与细菌致病性和耐药性密切相关.外膜各组分依赖特定的系统进行跨膜转运,包括脂多糖转运系统(lipopolysaccharide transport,Lpt)、脂质不对称维持系统(maintenance of lipid asymmetry,Mla)、β-桶状装配机器(β-barrel assembly machinery,Bam)以及脂蛋白定位系统(localization of lipoprotein,Lol).这些系统能够保证细菌外膜的完整与稳定,被视为维持细菌生命活动的"命门".因此,本文系统地综述革兰氏阴性细菌外膜主要成分的跨膜转运系统结构与功能,并对其未来研究方向进行展望,为新型靶向抗菌类药物研发提供新的思路.
