Based on the four dimensions of PCK,this paper investigates the professional development of rural PE teachers in Huaiji County by using the research methods of literature,questionnaire,interview and mathematical stati...Based on the four dimensions of PCK,this paper investigates the professional development of rural PE teachers in Huaiji County by using the research methods of literature,questionnaire,interview and mathematical statistics.Based on PCK related theories,this paper analyzes the current situation of professional development of rural PE teachers in Huaiji County,explores the problems existing in the process of professional development,and provides an optimal path for the professional development of rural PE teachers,so as to improve the teaching ability and classroom quality of rural PE teachers,and promote the further development of rural PE education under the background of new curriculum reform.Through the research,it is found that the shortcomings of rural PE teachers are:lack of situational knowledge;lack of understanding of the course content;not flexible use of teaching representation;low ability to identify the differences of students learning styles;lack of understanding of social environment,etc.展开更多
For evaluation of the rheological and mechanical properties of highly filled wood plastic composites （WPCs）, polypropylene/polyethylene （PP/PE） blends were grafted with maleic anhydride （MAH） to enhance the inte...For evaluation of the rheological and mechanical properties of highly filled wood plastic composites （WPCs）, polypropylene/polyethylene （PP/PE） blends were grafted with maleic anhydride （MAH） to enhance the interfacial adhesion between wood fiber and matrix. WPCs were prepared from wood fiber up to 60 wt.% and modified PP/PE was blended by extrusion. The rheological properties were studied by using dynamic measurement. According to the strain sweep test, the linear viscoelastic region of composites in the melt was determined. The result showed that the storage modulus was independent of the strain at low strain region （〈0.1%）. The frequency sweep resuits indicated that all composites exhibited shear thinning behavior, and both the storage modulus and complex viscosity of MAH modified composites were decreased comparing to those unmodified. Flexural properties and impact strength of the prepared WPCs were measured according to the relevant standard specifications. The flexural and impact strength of the manufactured composites significantly increased and reached a maximum when MAH dosage was 1.0 wt%, whereas the flexural modulus after an initial decreased, also increased with MAH dosage. The increase in mechanical properties indicated that the presence of anhydride groups enhanced the interracial adhesion between wood fiber and PP/PE blends.展开更多
A pre-column derivatization HPLC method for the determination of peimine(P)and peiminine(PE)in Bulbus Fritillariae has been developed. Under the derivatization conditions optimized,the calibration curve is Y1=-3.83...A pre-column derivatization HPLC method for the determination of peimine(P)and peiminine(PE)in Bulbus Fritillariae has been developed. Under the derivatization conditions optimized,the calibration curve is Y1=-3.83×103+1.33 ×105X1,r=0.998 for P and Y2=-7.86 × 102+6.33 × 104X2,r=0995 for PE,where Y is the peak area and X is the weight of the alkaloid; the average recovery is 98.0%(n=5, RSD=2.1%) for P and 101.0%(n=5,RSD=4.1%)for PE,the linear range is from 0.504 μg to 3.126μg for P and from 0.520μgto 3.328μgfor PE,respectively. Results of the determination of the two alkaloids in several samples of different Fritillaria species from various parts ofthe country are presented.The results suggest that P and PE are two major chemical constituents in bulbs of different Fritillaria species,and that the method developed is generally appli-cable to the determination of the hydroxy group on aliphatic fused ring systems without steric hin-drance.展开更多
A new kind of high performance composite resin matrix PEEK/PES, PEEKK/PES block copolymers have been prepared from the corresponding oligomers via a nucleophilic aromatic substitution reaction. The different propertie...A new kind of high performance composite resin matrix PEEK/PES, PEEKK/PES block copolymers have been prepared from the corresponding oligomers via a nucleophilic aromatic substitution reaction. The different properties of the copolymers are investigated by differential scanning calorimetry (d. s. c), thermogravimetric analysis (t. g. a) and dynamic mechanical analysis (d. m. a). The results show that the relationship between Tg and the compositions of the copolymers approximately follows the formula 1/Tg=W1/Tg1 +W2/Tg2 for PEEKK/PES block copolymers, and Tg=Tg1W1 +Tg2W2 for PEEK/PES block copolymers. The PES content and the segment length of the copolymers have a significant influence on their melting point. The thermal properties and dynamic mechanical behaviour of the copolymers are also studied. The introduction of PES segment into the molecular main chain increases the glass transition temperature of poly aryl ether ketones and decreases their melting temperature, that is to say it decreases their melting processing temperature. The block copolymers keep the high temperature stability and solvent resistance of poly aryl ether ketones. They are expected to be a new kind of high performance composite resin matrix.展开更多
A numerical procedure to determine the equivalent hydrodynamic dispersion coefficients and Péclet number（Pe） of a fractured rock is presented using random walk particle tracking method.The geometrical effects o...A numerical procedure to determine the equivalent hydrodynamic dispersion coefficients and Péclet number（Pe） of a fractured rock is presented using random walk particle tracking method.The geometrical effects of fracture system on hydrodynamic dispersion are studied.The results obtained from the proposed method agree well with those of empirical models,which are the scale-dependent hydrodynamic dispersion coefficients in an asymptotic or exponential form.A variance case is added to investigate the influence of longitudinal hydrodynamic dispersion in individual fractures on the macro-hydrodynamic dispersion at the fracture network scale,and its influence is demonstrated with a verification example.In addition,we investigate the influences of directional flow and stress conditions on the behavior of hydrodynamic dispersion in fracture networks.The results show that the magnitudes of the hydrodynamic dispersion coefficients are relatively smaller when the flow direction is parallel to the dip directions of fracture sets.Compressive stresses significantly reduce hydrodynamic dispersion.However,the remaining questions are：（1） whether the deformed fracture network under high stress conditions may make the scale-dependent hydrodynamic dispersion coefficients have asymptotic or exponential forms,and（2） what the conditions for existence of a well-defined equivalent hydrodynamic dispersion tensor are.They need to be further investigated.展开更多
The present work deals with the change in surface properties of polyethylene （PE） film using DC low pressure glow discharge air plasma and makes it useful for technical applications. The change in hydrophilicity of ...The present work deals with the change in surface properties of polyethylene （PE） film using DC low pressure glow discharge air plasma and makes it useful for technical applications. The change in hydrophilicity of the modified PE film surface was investigated by measuring contact angle and surface energy as a function of exposure time. Changes in the morphological and chemical composition of PE films were analyzed by atomic force microscopy （AFM） and X-ray photoelectron spectroscopy （XPS）. The improvement in adhesion was studied by measuring T-peel and lap-shear strength. The results show that the wettability and surface energy of the PE film has been improved due to the introduction of oxygen-containing polar groups and an increase in surface roughness. The XPS result clearly shows the increase in concentration of oxygen content and the formation of polar groups on the polymer surface. The AFM observation on PE film shows that the roughness of the surface increased due to plasma treatment. The above morphological and chemical changes enhanced the adhesive properties of the PE＇film surfaces, which was confirmed by T-peel and lap-shear tests.展开更多
Ultrafiltration membranes were prepared using phenolphthalein polyarylethersulfone (PES-C),polyethersulfone (PES) and poly(phthalazinone ether sulfone ketone) (PPESK) as polymers and NMP,DMAc,DMF and DMSO as solvents ...Ultrafiltration membranes were prepared using phenolphthalein polyarylethersulfone (PES-C),polyethersulfone (PES) and poly(phthalazinone ether sulfone ketone) (PPESK) as polymers and NMP,DMAc,DMF and DMSO as solvents by immersion precipitation via phase inversion.Experimental data of thermodynamic properties of the polymer solutions and kinetic process of membrane formation were reported.For polymer solutions with good solvents,the sequence of the viscous flow activation energy (E_η) was coincident with tha...展开更多
Phycoerythrin （PE） is one of the most important proteins involved in light capturing during photosynthesis in red algae. Its potential biological activities had gained wide concerns. In the present study, tumor cyto...Phycoerythrin （PE） is one of the most important proteins involved in light capturing during photosynthesis in red algae. Its potential biological activities had gained wide concerns. In the present study, tumor cytotoxic and hydroxyl radical assay were preformed to detect the bioactivity of recombinant PE. Recombinant plasmids pGEX-PE and pBGL were transformed into E.coli BL21 to make two recombinant strains BEX （pGEX-PE） and BGL （pBGL）. PE expressing in BEX （pGEX-PE） was validated by SDS-PAGE and Western blotting analysis. SDS-PAGE analysis indicated that the PE-GST fusion protein was mostly inclusion bodies. Specific expression of PE was confirmed by Western blotting analysis. The recombinant E. coli BEX （pGEX-PE） cells were collected and sonicated. The supernatants were reserved for the tumor cytotoxic experiments. The result of tumor cytotoxic assay indicated that the supernatants containing PE had the activity of inhibiting the growth of Hela cells and with the increase of protein concentration, the inhibiting rate increased from 37.31% to 63.26%, which showed significant difference from the control. Hydroxyl radical scavenging effect was tested with supernatants of BEX （pGEX-PE） and BGL （pBGL） cell lysates treated with sonication and heating. For the sonication samples, the scavenging rates of the supernatants of BEX （pGEX-PE） and BGL （pBGL） cell lysates were significantly higher than the negative control BL2 I（pGEX-4T） （P〈0.02）, and the scavenging rates increased slowly following the increase of the protein content. For the heating samples, except for the 0.2 mgmL^-1 BGL （pBGL） products, the scavenging effects of the supernatants of BEX （pGEX-PE） and BGL （pBGL） cell lysates were stronger than that of negative control BL21（pGEX-4T）. However, the effect intensity was not positively correlated with the increase of the protein concentration. Though a partially decreased hydroxyl radical scavenging activity was led by heating, the biological activity was still retained and conspicuous. This research showed that phycoerythrin protein expressing in E. coli has the potential medical and sanitarian value.展开更多
Spherical polyethylene/polypropylene (PE/PP) in-reactor blend granules with various ethylene/propylene molar ratios and high porosity were synthesized using a high yield TiCVMgCl2 supported catalyst. A solution of ben...Spherical polyethylene/polypropylene (PE/PP) in-reactor blend granules with various ethylene/propylene molar ratios and high porosity were synthesized using a high yield TiCVMgCl2 supported catalyst. A solution of benzoyl peroxide (BPO)/maleic anhydride (MAH)/xylene (interfacial reagent) or BPO/MAH/St (comonomer) was absorbed onto the PE/PP in- reactor blend granules, and solid phase graft polymerization of MAH on PE/PP was conducted. The amount of grafted MAH on PE/PP was measured through chemical titration. The results showed that solid phase graft polymerization of MAH in PE/PP in-reactor blend granules produced graft copolymer with high amount of grafted MAH, and the amount of grafted MAH was raised slightly when St was introduced as comonomer. The graft in-reactor blend was fractionated into five fractions through temperature-gradient extraction fractionation (TGEF), and the fractions were analyzed by FTIR. The results revealed that MAH is mainly grafted on the PE segments, whereas MAH was predominantly grafted on the PP segments when St was present in the graft polymerization system. In addition, the final product is still in the form of regular spherical granules, which is beneficial for industrial processing.展开更多
Density functional theory （DFT） calculations are employed to investigate the structural and electronic properties of MoS6^- and MoS6 clusters. Generalized Koopmans＇ theorem is applied to predict the vertical detach...Density functional theory （DFT） calculations are employed to investigate the structural and electronic properties of MoS6^- and MoS6 clusters. Generalized Koopmans＇ theorem is applied to predict the vertical detachment energies and simulate the photoelectron spectra （PES）. Intriguingly, the terminal S2-, polysulfide S2^2- and S3^2- ligands simultaneously emerge in the lowest-energy structure of MoS6. Molecular orbital analyses are performed to analyze the chemical bonding in MoS6^-/0 clusters and elucidate their structural and electronic properties.展开更多
基金Youth Innovation Talent Project of Guangdong Department of Education(2019WQNCX120)Key Project of Zhaoqing Education Development Research Institute(ZQJYY2016003)Quality Engineering and Teaching Reform Project of Zhaoqing University(zlgc201860).
文摘Based on the four dimensions of PCK,this paper investigates the professional development of rural PE teachers in Huaiji County by using the research methods of literature,questionnaire,interview and mathematical statistics.Based on PCK related theories,this paper analyzes the current situation of professional development of rural PE teachers in Huaiji County,explores the problems existing in the process of professional development,and provides an optimal path for the professional development of rural PE teachers,so as to improve the teaching ability and classroom quality of rural PE teachers,and promote the further development of rural PE education under the background of new curriculum reform.Through the research,it is found that the shortcomings of rural PE teachers are:lack of situational knowledge;lack of understanding of the course content;not flexible use of teaching representation;low ability to identify the differences of students learning styles;lack of understanding of social environment,etc.
文摘For evaluation of the rheological and mechanical properties of highly filled wood plastic composites （WPCs）, polypropylene/polyethylene （PP/PE） blends were grafted with maleic anhydride （MAH） to enhance the interfacial adhesion between wood fiber and matrix. WPCs were prepared from wood fiber up to 60 wt.% and modified PP/PE was blended by extrusion. The rheological properties were studied by using dynamic measurement. According to the strain sweep test, the linear viscoelastic region of composites in the melt was determined. The result showed that the storage modulus was independent of the strain at low strain region （〈0.1%）. The frequency sweep resuits indicated that all composites exhibited shear thinning behavior, and both the storage modulus and complex viscosity of MAH modified composites were decreased comparing to those unmodified. Flexural properties and impact strength of the prepared WPCs were measured according to the relevant standard specifications. The flexural and impact strength of the manufactured composites significantly increased and reached a maximum when MAH dosage was 1.0 wt%, whereas the flexural modulus after an initial decreased, also increased with MAH dosage. The increase in mechanical properties indicated that the presence of anhydride groups enhanced the interracial adhesion between wood fiber and PP/PE blends.
文摘A pre-column derivatization HPLC method for the determination of peimine(P)and peiminine(PE)in Bulbus Fritillariae has been developed. Under the derivatization conditions optimized,the calibration curve is Y1=-3.83×103+1.33 ×105X1,r=0.998 for P and Y2=-7.86 × 102+6.33 × 104X2,r=0995 for PE,where Y is the peak area and X is the weight of the alkaloid; the average recovery is 98.0%(n=5, RSD=2.1%) for P and 101.0%(n=5,RSD=4.1%)for PE,the linear range is from 0.504 μg to 3.126μg for P and from 0.520μgto 3.328μgfor PE,respectively. Results of the determination of the two alkaloids in several samples of different Fritillaria species from various parts ofthe country are presented.The results suggest that P and PE are two major chemical constituents in bulbs of different Fritillaria species,and that the method developed is generally appli-cable to the determination of the hydroxy group on aliphatic fused ring systems without steric hin-drance.
文摘A new kind of high performance composite resin matrix PEEK/PES, PEEKK/PES block copolymers have been prepared from the corresponding oligomers via a nucleophilic aromatic substitution reaction. The different properties of the copolymers are investigated by differential scanning calorimetry (d. s. c), thermogravimetric analysis (t. g. a) and dynamic mechanical analysis (d. m. a). The results show that the relationship between Tg and the compositions of the copolymers approximately follows the formula 1/Tg=W1/Tg1 +W2/Tg2 for PEEKK/PES block copolymers, and Tg=Tg1W1 +Tg2W2 for PEEK/PES block copolymers. The PES content and the segment length of the copolymers have a significant influence on their melting point. The thermal properties and dynamic mechanical behaviour of the copolymers are also studied. The introduction of PES segment into the molecular main chain increases the glass transition temperature of poly aryl ether ketones and decreases their melting temperature, that is to say it decreases their melting processing temperature. The block copolymers keep the high temperature stability and solvent resistance of poly aryl ether ketones. They are expected to be a new kind of high performance composite resin matrix.
基金the financial supports from Swedish Nuclear Fuel and Waste Management Co.(SKB) through the DECOVALEX-2011 project
文摘A numerical procedure to determine the equivalent hydrodynamic dispersion coefficients and Péclet number（Pe） of a fractured rock is presented using random walk particle tracking method.The geometrical effects of fracture system on hydrodynamic dispersion are studied.The results obtained from the proposed method agree well with those of empirical models,which are the scale-dependent hydrodynamic dispersion coefficients in an asymptotic or exponential form.A variance case is added to investigate the influence of longitudinal hydrodynamic dispersion in individual fractures on the macro-hydrodynamic dispersion at the fracture network scale,and its influence is demonstrated with a verification example.In addition,we investigate the influences of directional flow and stress conditions on the behavior of hydrodynamic dispersion in fracture networks.The results show that the magnitudes of the hydrodynamic dispersion coefficients are relatively smaller when the flow direction is parallel to the dip directions of fracture sets.Compressive stresses significantly reduce hydrodynamic dispersion.However,the remaining questions are：（1） whether the deformed fracture network under high stress conditions may make the scale-dependent hydrodynamic dispersion coefficients have asymptotic or exponential forms,and（2） what the conditions for existence of a well-defined equivalent hydrodynamic dispersion tensor are.They need to be further investigated.
文摘The present work deals with the change in surface properties of polyethylene （PE） film using DC low pressure glow discharge air plasma and makes it useful for technical applications. The change in hydrophilicity of the modified PE film surface was investigated by measuring contact angle and surface energy as a function of exposure time. Changes in the morphological and chemical composition of PE films were analyzed by atomic force microscopy （AFM） and X-ray photoelectron spectroscopy （XPS）. The improvement in adhesion was studied by measuring T-peel and lap-shear strength. The results show that the wettability and surface energy of the PE film has been improved due to the introduction of oxygen-containing polar groups and an increase in surface roughness. The XPS result clearly shows the increase in concentration of oxygen content and the formation of polar groups on the polymer surface. The AFM observation on PE film shows that the roughness of the surface increased due to plasma treatment. The above morphological and chemical changes enhanced the adhesive properties of the PE＇film surfaces, which was confirmed by T-peel and lap-shear tests.
基金supported by the Major State Basic Research Program of China (No.2009CB623404)National Natural Science Foundation of China (Nos.20736003,20676067)+1 种基金National High Technology Research and Development Program of China (No.2007AA06Z317)Foundation of Ministry of Education of China (No.20070003130).
文摘Ultrafiltration membranes were prepared using phenolphthalein polyarylethersulfone (PES-C),polyethersulfone (PES) and poly(phthalazinone ether sulfone ketone) (PPESK) as polymers and NMP,DMAc,DMF and DMSO as solvents by immersion precipitation via phase inversion.Experimental data of thermodynamic properties of the polymer solutions and kinetic process of membrane formation were reported.For polymer solutions with good solvents,the sequence of the viscous flow activation energy (E_η) was coincident with tha...
文摘Phycoerythrin （PE） is one of the most important proteins involved in light capturing during photosynthesis in red algae. Its potential biological activities had gained wide concerns. In the present study, tumor cytotoxic and hydroxyl radical assay were preformed to detect the bioactivity of recombinant PE. Recombinant plasmids pGEX-PE and pBGL were transformed into E.coli BL21 to make two recombinant strains BEX （pGEX-PE） and BGL （pBGL）. PE expressing in BEX （pGEX-PE） was validated by SDS-PAGE and Western blotting analysis. SDS-PAGE analysis indicated that the PE-GST fusion protein was mostly inclusion bodies. Specific expression of PE was confirmed by Western blotting analysis. The recombinant E. coli BEX （pGEX-PE） cells were collected and sonicated. The supernatants were reserved for the tumor cytotoxic experiments. The result of tumor cytotoxic assay indicated that the supernatants containing PE had the activity of inhibiting the growth of Hela cells and with the increase of protein concentration, the inhibiting rate increased from 37.31% to 63.26%, which showed significant difference from the control. Hydroxyl radical scavenging effect was tested with supernatants of BEX （pGEX-PE） and BGL （pBGL） cell lysates treated with sonication and heating. For the sonication samples, the scavenging rates of the supernatants of BEX （pGEX-PE） and BGL （pBGL） cell lysates were significantly higher than the negative control BL2 I（pGEX-4T） （P〈0.02）, and the scavenging rates increased slowly following the increase of the protein content. For the heating samples, except for the 0.2 mgmL^-1 BGL （pBGL） products, the scavenging effects of the supernatants of BEX （pGEX-PE） and BGL （pBGL） cell lysates were stronger than that of negative control BL21（pGEX-4T）. However, the effect intensity was not positively correlated with the increase of the protein concentration. Though a partially decreased hydroxyl radical scavenging activity was led by heating, the biological activity was still retained and conspicuous. This research showed that phycoerythrin protein expressing in E. coli has the potential medical and sanitarian value.
基金This work was supported by the Special Fund for Major State Basic Research Projects (Grant no. G1999064803).
文摘Spherical polyethylene/polypropylene (PE/PP) in-reactor blend granules with various ethylene/propylene molar ratios and high porosity were synthesized using a high yield TiCVMgCl2 supported catalyst. A solution of benzoyl peroxide (BPO)/maleic anhydride (MAH)/xylene (interfacial reagent) or BPO/MAH/St (comonomer) was absorbed onto the PE/PP in- reactor blend granules, and solid phase graft polymerization of MAH on PE/PP was conducted. The amount of grafted MAH on PE/PP was measured through chemical titration. The results showed that solid phase graft polymerization of MAH in PE/PP in-reactor blend granules produced graft copolymer with high amount of grafted MAH, and the amount of grafted MAH was raised slightly when St was introduced as comonomer. The graft in-reactor blend was fractionated into five fractions through temperature-gradient extraction fractionation (TGEF), and the fractions were analyzed by FTIR. The results revealed that MAH is mainly grafted on the PE segments, whereas MAH was predominantly grafted on the PP segments when St was present in the graft polymerization system. In addition, the final product is still in the form of regular spherical granules, which is beneficial for industrial processing.
基金Supported by the National Natural Science Foundation of China (21171039, 21073035, 21071031 and 90922022)National Science Foundation for Fostering Talents in Basic Research of the National Natural Science Foundation of China (J1103303)Foundation of Fuzhou University (0460-022342 and 0041-600616)
文摘Density functional theory （DFT） calculations are employed to investigate the structural and electronic properties of MoS6^- and MoS6 clusters. Generalized Koopmans＇ theorem is applied to predict the vertical detachment energies and simulate the photoelectron spectra （PES）. Intriguingly, the terminal S2-, polysulfide S2^2- and S3^2- ligands simultaneously emerge in the lowest-energy structure of MoS6. Molecular orbital analyses are performed to analyze the chemical bonding in MoS6^-/0 clusters and elucidate their structural and electronic properties.
