Background: Recent studies show increasing prostate cancer incidence in Nigeria. Significant correlations identified between diet and prostate cancer occurrence, indicate that low antioxidant status could contribute t...Background: Recent studies show increasing prostate cancer incidence in Nigeria. Significant correlations identified between diet and prostate cancer occurrence, indicate that low antioxidant status could contribute to the aetiology of prostate cancer. Methods: This cross-sectional study determined selected antioxidants (lycopene, beta-carotene and retinol) status of 10 (Experimental group) and 17 (Control) male adults with and without PC, recruited from the Urological Section of the Surgical Out-patients’ Department, University College Hospital and the General Out-patient Clinic of the Ring Road State Hospital, Ibadan, Oyo State, Nigeria. Demographic characteristics were assessed using a semi-structured, interviewer-administered questionnaire. Daily antioxidant intakes were assessed and analyzed using 24-hour dietary recall, and an adapted version of the food database “Total Diet Assessment”. Serum lycopene, beta-carotene and retinol were determined using High Performance Liquid Chromatography. Results: The mean age of the PC and the control (72.8 ± 6.2 years and 59.8 ± 4.8 years) was significantly different (p = 0.001). Mean daily lycopene intake (1408.4 ± 233.2 μg) of the PC was significantly lower (P = 0.030) than the controls (3862.3±316.2μg). The mean serum lycopene (19.8 ± 13.2 ng/ml), beta-carotene (43.6 ± 26.0 ng/ml) and retinol (362.2 ± 304.3ng/ml) of the PC were significantly lower (p = 0.008, 0.040 and 0.033 respectively) than the values (70.8 ± 49.8 ng/ml, 57.6 ± 47.7 ng/ml and 395.4 ± 275.6 ng/ml respectively) of the controls. Significant inverse correlations were observed between the dietary lycopene intake (r = -0.396, p = 0.041) and serum lycopene (r =-0.502, p = 0.008) with PC;while a significant positive association was observed between dietary intake of retinol with PC (r = 0.394, p = 0.042). Conclusion: The study has revealed low anti-oxidant status, and an inverse association between lycopene status and prostate cancer in the elderly men.展开更多
Background: Long-term remote ischemic conditioning (RIC) has been proven to be beneficial in multiple diseases, such as cerebral and cardiovascular diseases. However, the hyperacute and acute effects of a single RIC s...Background: Long-term remote ischemic conditioning (RIC) has been proven to be beneficial in multiple diseases, such as cerebral and cardiovascular diseases. However, the hyperacute and acute effects of a single RIC stimulus are still not clear. Quantitative proteomic analyses of plasma proteins following RIC application have been conducted in preclinical and clinical studies but exhibit high heterogeneity in results due to wide variations in experimental setups and sampling procedures. Hence, this study aimed to explore the immediate effects of RIC on plasma proteome in healthy young adults to exclude confounding factors of disease entity, such as medications and gender. Methods: Young healthy male participants were enrolled after a systematic physical examination and 6-month lifestyle observation. Individual RIC sessions included five cycles of alternative ischemia and reperfusion, each lasting for 5 min in bilateral forearms. Blood samples were collected at baseline, 5 min after RIC, and 2 h after RIC, and then samples were processed for proteomic analysis using liquid chromatography-tandem mass spectrometry method. Results: Proteins related to lipid metabolism (e.g., Apolipoprotein F), coagulation factors (hepatocyte growth factor activator preproprotein), members of complement cascades (mannan-binding lectin serine protease 1 isoform 2 precursor), and inflammatory responses (carboxypeptidase N catalytic chain precursor) were differentially altered at their serum levels following the RIC intervention. The most enriched pathways were protein glycosylation and complement/coagulation cascades. Conclusions: One-time RIC stimulus may induce instant cellular responses like anti-inflammation, coagulation, and fibrinolysis balancing, and lipid metabolism regulation which are protective in different perspectives. Protective effects of single RIC in hyperacute and acute phases may be exploited in clinical emergency settings due to apparently beneficial alterations in plasma proteome profile. Furthermore, the beneficial effects of long-term (repeated) RIC interventions in preventing chronic cardiovascular diseases among general populations can also be expected based on our study findings.展开更多
The concentrations of 16 phthalates in 164 commercial Chinese rice wines （CRW） were detected by GC-MS, and consumption data on CRW in different packaging types was investigated from 634 adult males in Shanghai using...The concentrations of 16 phthalates in 164 commercial Chinese rice wines （CRW） were detected by GC-MS, and consumption data on CRW in different packaging types was investigated from 634 adult males in Shanghai using a food frequency questionnaire. Based on the prinq：iples of probabilistic modelling and cumulative risk assessment, the exposure and health risk of phthalates from CRW to adult males in Shanghai was evaluated. DMP, DEP, DIBP, DnBP, BBP, and DEHP were detected in the samples, thE： range of detection frequency of individual phthalates varied from 6.10% for BBP to 15.24% for DIBP, and the detected concentrations were 51.06-200.34 ng/mL. All the respondents consumed CRW, 90.69% of them consumed CRW 0.01-49.9 mL/d, the minimum value of the average daily intake of CRW was 6.25 mL/d, the median was 13.72 mL/d and the maximum was 300 mL/d. The median exposure level of the 6 detected Phthalates to adult males in Shanghai were 6.58-7.10 ng/（d.kg）, and the maximum exposure level were 137.38-540.47 ng/（d.kg）. The cumulative exposure health risk index （HI） based on the median and maximum exposure level of the 6 Phthalates （DMP, I~）EP, DIBP, DnBP, BBP, and DEHP） were 0.001147 ancl 0.063396, both were far less than 1. In conclusion, CRW were generally consumed by the adult males in Shanghai.展开更多
Background: Urinary retention is one of the common urologic emergencies constituting a significant workload of urologists and non-urologists alike. Aims and Objectives: This study was undertaken to identify the causes...Background: Urinary retention is one of the common urologic emergencies constituting a significant workload of urologists and non-urologists alike. Aims and Objectives: This study was undertaken to identify the causes and complications associated with urinary retention in adult male patients at AKTH, Kano. Patients and Methods: It was a prospective hospital-based study of 110 consecutive adult male patients who presented to Aminu Kano Teaching Hospital, Kano with urinary retention over 12 months. On presentation, a brief history was taken and rapid physical examination done;the urinary retention was relieved by urethral catheterization or suprapubic cystostomy. A detailed history and thorough physical examination findings were later obtained. Results: One hundred and ten patients were seen during the study period. There ages ranged from 17 to100 years with a mean of 56 ± 19.3 SD years. Patients within the age range 55 - 74 years accounted for the largest group, (25.5% + 23.6% = 49.1%). The most common cause of urinary retention in this study was benign prostatic hyperplasia (51.8% of the patients). This was followed by urethral stricture (20%), cancer of the prostate (7.3%), urethral injury (7.3%) and bladder tumour (6.4%). More than half of the patients (53.4%) presented with acute urinary retention, 30.5% presented with chronic urinary retention, and 16.1% were diagnosed to have acute-on-chronic urinary retention. The complications of urinary retention found were: urinary tract infection (in 24.5% of patients), renal impairment (14.5%), and anaemia (11.8%). Conclusion: Urinary retention is commoner among the middle aged and the elderly. Benign prostatic hyperplasia remains the leading cause of urinary retention. Acute urinary retention was the commonest type of urinary retention;however, complications due to the retention were associated with chronic and acute-on-chronic urinary retention.展开更多
目的:探讨不同体重指数(body mass index,BMI)青年男性静息能量消耗(resting energy expenditure,REE)的特点,比较常见静息代谢率(resting metabolic rate,RMR)预测方程与间接测热法的差异。方法:以2017年12月至2021年6月于北京大学第...目的:探讨不同体重指数(body mass index,BMI)青年男性静息能量消耗(resting energy expenditure,REE)的特点,比较常见静息代谢率(resting metabolic rate,RMR)预测方程与间接测热法的差异。方法:以2017年12月至2021年6月于北京大学第三医院运动医学科进行静息代谢测试的30名青年男性为研究对象,采用间接测热法测定RMR,采用生物电阻抗法测定体成分。分析研究对象REE的特点,并与11个常见预测方程的推算值进行比较,通过配对t检验和组内相关系数(intra-class correlation coefficient,ICC)评估两者差异。结果:30名青年男性的平均年龄为(26.93±4.16)岁,整体RMR为(1960.17±463.11)kcal/d(1 kcal=4.1868 kJ),其中BMI正常者的RMR为(1744.33±249.62)kcal/d,显著低于超重或肥胖者[(2104.06±520.32)kcal/d](P<0.01),但体质量校正后,BMI正常者的RMR显著高于超重或肥胖者[(24.02±2.61)kcal/(kg·d)vs.(19.98±4.38)kcal/(kg·d),P<0.01];不同BMI受试者的RMR与体质量、脂肪量、去脂体重、体表面积、细胞外液呈显著正相关(P均<0.05)。11个预测方程的预测值与实测值的一致性均不佳(ICC均<0.75),其中,超重或肥胖青年男性采用世界卫生组织(World Health Organization,WHO)推荐使用的RMR预测方程的预测值与实测值的一致性相对较高(ICC=0.547,P<0.01)。结论:不同BMI青年男性的RMR存在显著差异,超重或肥胖者要考虑体质量矫正后的RMR情况。不同预测方程的预测值与RMR的实测值一致性较差,建议通过间接测热法准确测定RMR。对于超重和肥胖的青年男性可以考虑采用WHO推荐使用的预测方程计算RMR,但有必要建立适用于不同BMI人群的RMR预测方程。展开更多
Soluble male worm antigen of Schistosoma japonicum ( Sj ) was investigated for development of new vaccine candidate. SDS-PAGE and Western blotting were performed to compare the difference between soluble antigens from...Soluble male worm antigen of Schistosoma japonicum ( Sj ) was investigated for development of new vaccine candidate. SDS-PAGE and Western blotting were performed to compare the difference between soluble antigens from worms of different sex. Mice vaccination with the testing purified protein was followed by Sj cercariae challenge to detect the protective effect against Sj . Sixteen bands were seen for the soluble male worm antigen and 12 for the female worm. In addition, a distinct band of 44.6 kDa from the male worm antigen was observed, and its antigenicity was demonstrated by Western blotting. This 44.6 kDa protein could induce significant worm and egg reduction rate in mice (39.31%, 41.98%, P <0.001). In this study a 44.6 kDa protein was isolated and partially characterized. Its antigenicity, immunogenicity and the partial immune protection suggest its potential vaccine candidte against Sj .展开更多
AIM To determine whether recent evidence-based United States polices on male circumcision(MC) apply to comparable Anglophone countries,Australia and New Zealand.METHODS Articles in 2005 through 2015 were retrieved fro...AIM To determine whether recent evidence-based United States polices on male circumcision(MC) apply to comparable Anglophone countries,Australia and New Zealand.METHODS Articles in 2005 through 2015 were retrieved from PubM ed using the keyword "circumcision" together with 36 relevant subtopics.A further PubM ed search was performed for articles published in 2016.Searches of the EMBASE and Cochrane databases did not yield additional citable articles.Articles were assessed for quality and those rated 2+ and above according to the Scottish Intercollegiate Grading System were studied further.The most relevant andrepresentative of the topic were included.Bibliographies were examined to retrieve further key references.Randomized controlled trials,recent high quality systematic reviews or meta-analyses(level 1++ or 1+ evidence) were prioritized for inclusion.A risk-benefit analysis of articles rated for quality was performed.For efficiency and reliability,recent randomized controlled trials,metaanalyses,high quality systematic reviews and large welldesigned studies were used if available.Internet searches were conducted for other relevant information,including policies and Australian data on claims under Medicare for MC.RESULTS Evidence-based policy statements by the American Academy of Pediatrics(AAP) and the Centers for Disease Control and Prevention(CDC) support infant and later age male circumcision(MC) as a desirable public health measure.Our systematic review of relevant literature over the past decade yielded 140 journal articles that met our inclusion criteria.Together,these showed that early infant MC confers immediate and lifelong benefits by protecting against urinary tract infections having potential adverse long-term renal effects,phimosis that causes difficult and painful erections and "ballooning" during urination,inflammatory skin conditions,inferior penile hygiene,candidiasis,various sexually transmissible infections in both sexes,genital ulcers,and penile,prostate and cervical cancer.Our risk-benefit analysis showed that benefits exceeded procedural risks,which are predominantly minor,by up to 200 to 1.We estimated that more than 1 in 2 uncircumcised males will experience an adverse foreskin-related medical condition over their lifetime.Wide-ranging evidence from surveys,physiological measurements,and the anatomical location of penile sensory receptors responsible for sexual sensation strongly and consistently suggested that MC has no detrimental effect on sexual function,sensitivity or pleasure.United States studies showed that early infant MC is cost saving.The evidence supporting early infant MC has further strengthened since the positive AAP and CDC reviews.CONCLUSION Affirmative MC policies are needed in Australia and New Zealand.Routine provision of accurate,unbiased education,and access in public hospitals,will maximize health and financial benefits.展开更多
文摘Background: Recent studies show increasing prostate cancer incidence in Nigeria. Significant correlations identified between diet and prostate cancer occurrence, indicate that low antioxidant status could contribute to the aetiology of prostate cancer. Methods: This cross-sectional study determined selected antioxidants (lycopene, beta-carotene and retinol) status of 10 (Experimental group) and 17 (Control) male adults with and without PC, recruited from the Urological Section of the Surgical Out-patients’ Department, University College Hospital and the General Out-patient Clinic of the Ring Road State Hospital, Ibadan, Oyo State, Nigeria. Demographic characteristics were assessed using a semi-structured, interviewer-administered questionnaire. Daily antioxidant intakes were assessed and analyzed using 24-hour dietary recall, and an adapted version of the food database “Total Diet Assessment”. Serum lycopene, beta-carotene and retinol were determined using High Performance Liquid Chromatography. Results: The mean age of the PC and the control (72.8 ± 6.2 years and 59.8 ± 4.8 years) was significantly different (p = 0.001). Mean daily lycopene intake (1408.4 ± 233.2 μg) of the PC was significantly lower (P = 0.030) than the controls (3862.3±316.2μg). The mean serum lycopene (19.8 ± 13.2 ng/ml), beta-carotene (43.6 ± 26.0 ng/ml) and retinol (362.2 ± 304.3ng/ml) of the PC were significantly lower (p = 0.008, 0.040 and 0.033 respectively) than the values (70.8 ± 49.8 ng/ml, 57.6 ± 47.7 ng/ml and 395.4 ± 275.6 ng/ml respectively) of the controls. Significant inverse correlations were observed between the dietary lycopene intake (r = -0.396, p = 0.041) and serum lycopene (r =-0.502, p = 0.008) with PC;while a significant positive association was observed between dietary intake of retinol with PC (r = 0.394, p = 0.042). Conclusion: The study has revealed low anti-oxidant status, and an inverse association between lycopene status and prostate cancer in the elderly men.
基金supported by the National Key R&D Program of China(2017YFC1308400)the National Natural Science Foundation(81371289)+1 种基金and the Beijing Natural Science Foundation(7212047)and Capital Medical Development Scientific Research Fund(2022-2-2015).
文摘Background: Long-term remote ischemic conditioning (RIC) has been proven to be beneficial in multiple diseases, such as cerebral and cardiovascular diseases. However, the hyperacute and acute effects of a single RIC stimulus are still not clear. Quantitative proteomic analyses of plasma proteins following RIC application have been conducted in preclinical and clinical studies but exhibit high heterogeneity in results due to wide variations in experimental setups and sampling procedures. Hence, this study aimed to explore the immediate effects of RIC on plasma proteome in healthy young adults to exclude confounding factors of disease entity, such as medications and gender. Methods: Young healthy male participants were enrolled after a systematic physical examination and 6-month lifestyle observation. Individual RIC sessions included five cycles of alternative ischemia and reperfusion, each lasting for 5 min in bilateral forearms. Blood samples were collected at baseline, 5 min after RIC, and 2 h after RIC, and then samples were processed for proteomic analysis using liquid chromatography-tandem mass spectrometry method. Results: Proteins related to lipid metabolism (e.g., Apolipoprotein F), coagulation factors (hepatocyte growth factor activator preproprotein), members of complement cascades (mannan-binding lectin serine protease 1 isoform 2 precursor), and inflammatory responses (carboxypeptidase N catalytic chain precursor) were differentially altered at their serum levels following the RIC intervention. The most enriched pathways were protein glycosylation and complement/coagulation cascades. Conclusions: One-time RIC stimulus may induce instant cellular responses like anti-inflammation, coagulation, and fibrinolysis balancing, and lipid metabolism regulation which are protective in different perspectives. Protective effects of single RIC in hyperacute and acute phases may be exploited in clinical emergency settings due to apparently beneficial alterations in plasma proteome profile. Furthermore, the beneficial effects of long-term (repeated) RIC interventions in preventing chronic cardiovascular diseases among general populations can also be expected based on our study findings.
基金supported by Natural Science Foundation of China(81202208 and 81172673)Morningstar research projects in Shanghai Business School(A-0201-00-050-106)
文摘The concentrations of 16 phthalates in 164 commercial Chinese rice wines （CRW） were detected by GC-MS, and consumption data on CRW in different packaging types was investigated from 634 adult males in Shanghai using a food frequency questionnaire. Based on the prinq：iples of probabilistic modelling and cumulative risk assessment, the exposure and health risk of phthalates from CRW to adult males in Shanghai was evaluated. DMP, DEP, DIBP, DnBP, BBP, and DEHP were detected in the samples, thE： range of detection frequency of individual phthalates varied from 6.10% for BBP to 15.24% for DIBP, and the detected concentrations were 51.06-200.34 ng/mL. All the respondents consumed CRW, 90.69% of them consumed CRW 0.01-49.9 mL/d, the minimum value of the average daily intake of CRW was 6.25 mL/d, the median was 13.72 mL/d and the maximum was 300 mL/d. The median exposure level of the 6 detected Phthalates to adult males in Shanghai were 6.58-7.10 ng/（d.kg）, and the maximum exposure level were 137.38-540.47 ng/（d.kg）. The cumulative exposure health risk index （HI） based on the median and maximum exposure level of the 6 Phthalates （DMP, I~）EP, DIBP, DnBP, BBP, and DEHP） were 0.001147 ancl 0.063396, both were far less than 1. In conclusion, CRW were generally consumed by the adult males in Shanghai.
文摘Background: Urinary retention is one of the common urologic emergencies constituting a significant workload of urologists and non-urologists alike. Aims and Objectives: This study was undertaken to identify the causes and complications associated with urinary retention in adult male patients at AKTH, Kano. Patients and Methods: It was a prospective hospital-based study of 110 consecutive adult male patients who presented to Aminu Kano Teaching Hospital, Kano with urinary retention over 12 months. On presentation, a brief history was taken and rapid physical examination done;the urinary retention was relieved by urethral catheterization or suprapubic cystostomy. A detailed history and thorough physical examination findings were later obtained. Results: One hundred and ten patients were seen during the study period. There ages ranged from 17 to100 years with a mean of 56 ± 19.3 SD years. Patients within the age range 55 - 74 years accounted for the largest group, (25.5% + 23.6% = 49.1%). The most common cause of urinary retention in this study was benign prostatic hyperplasia (51.8% of the patients). This was followed by urethral stricture (20%), cancer of the prostate (7.3%), urethral injury (7.3%) and bladder tumour (6.4%). More than half of the patients (53.4%) presented with acute urinary retention, 30.5% presented with chronic urinary retention, and 16.1% were diagnosed to have acute-on-chronic urinary retention. The complications of urinary retention found were: urinary tract infection (in 24.5% of patients), renal impairment (14.5%), and anaemia (11.8%). Conclusion: Urinary retention is commoner among the middle aged and the elderly. Benign prostatic hyperplasia remains the leading cause of urinary retention. Acute urinary retention was the commonest type of urinary retention;however, complications due to the retention were associated with chronic and acute-on-chronic urinary retention.
文摘目的:探讨不同体重指数(body mass index,BMI)青年男性静息能量消耗(resting energy expenditure,REE)的特点,比较常见静息代谢率(resting metabolic rate,RMR)预测方程与间接测热法的差异。方法:以2017年12月至2021年6月于北京大学第三医院运动医学科进行静息代谢测试的30名青年男性为研究对象,采用间接测热法测定RMR,采用生物电阻抗法测定体成分。分析研究对象REE的特点,并与11个常见预测方程的推算值进行比较,通过配对t检验和组内相关系数(intra-class correlation coefficient,ICC)评估两者差异。结果:30名青年男性的平均年龄为(26.93±4.16)岁,整体RMR为(1960.17±463.11)kcal/d(1 kcal=4.1868 kJ),其中BMI正常者的RMR为(1744.33±249.62)kcal/d,显著低于超重或肥胖者[(2104.06±520.32)kcal/d](P<0.01),但体质量校正后,BMI正常者的RMR显著高于超重或肥胖者[(24.02±2.61)kcal/(kg·d)vs.(19.98±4.38)kcal/(kg·d),P<0.01];不同BMI受试者的RMR与体质量、脂肪量、去脂体重、体表面积、细胞外液呈显著正相关(P均<0.05)。11个预测方程的预测值与实测值的一致性均不佳(ICC均<0.75),其中,超重或肥胖青年男性采用世界卫生组织(World Health Organization,WHO)推荐使用的RMR预测方程的预测值与实测值的一致性相对较高(ICC=0.547,P<0.01)。结论:不同BMI青年男性的RMR存在显著差异,超重或肥胖者要考虑体质量矫正后的RMR情况。不同预测方程的预测值与RMR的实测值一致性较差,建议通过间接测热法准确测定RMR。对于超重和肥胖的青年男性可以考虑采用WHO推荐使用的预测方程计算RMR,但有必要建立适用于不同BMI人群的RMR预测方程。
文摘Soluble male worm antigen of Schistosoma japonicum ( Sj ) was investigated for development of new vaccine candidate. SDS-PAGE and Western blotting were performed to compare the difference between soluble antigens from worms of different sex. Mice vaccination with the testing purified protein was followed by Sj cercariae challenge to detect the protective effect against Sj . Sixteen bands were seen for the soluble male worm antigen and 12 for the female worm. In addition, a distinct band of 44.6 kDa from the male worm antigen was observed, and its antigenicity was demonstrated by Western blotting. This 44.6 kDa protein could induce significant worm and egg reduction rate in mice (39.31%, 41.98%, P <0.001). In this study a 44.6 kDa protein was isolated and partially characterized. Its antigenicity, immunogenicity and the partial immune protection suggest its potential vaccine candidte against Sj .
文摘AIM To determine whether recent evidence-based United States polices on male circumcision(MC) apply to comparable Anglophone countries,Australia and New Zealand.METHODS Articles in 2005 through 2015 were retrieved from PubM ed using the keyword "circumcision" together with 36 relevant subtopics.A further PubM ed search was performed for articles published in 2016.Searches of the EMBASE and Cochrane databases did not yield additional citable articles.Articles were assessed for quality and those rated 2+ and above according to the Scottish Intercollegiate Grading System were studied further.The most relevant andrepresentative of the topic were included.Bibliographies were examined to retrieve further key references.Randomized controlled trials,recent high quality systematic reviews or meta-analyses(level 1++ or 1+ evidence) were prioritized for inclusion.A risk-benefit analysis of articles rated for quality was performed.For efficiency and reliability,recent randomized controlled trials,metaanalyses,high quality systematic reviews and large welldesigned studies were used if available.Internet searches were conducted for other relevant information,including policies and Australian data on claims under Medicare for MC.RESULTS Evidence-based policy statements by the American Academy of Pediatrics(AAP) and the Centers for Disease Control and Prevention(CDC) support infant and later age male circumcision(MC) as a desirable public health measure.Our systematic review of relevant literature over the past decade yielded 140 journal articles that met our inclusion criteria.Together,these showed that early infant MC confers immediate and lifelong benefits by protecting against urinary tract infections having potential adverse long-term renal effects,phimosis that causes difficult and painful erections and "ballooning" during urination,inflammatory skin conditions,inferior penile hygiene,candidiasis,various sexually transmissible infections in both sexes,genital ulcers,and penile,prostate and cervical cancer.Our risk-benefit analysis showed that benefits exceeded procedural risks,which are predominantly minor,by up to 200 to 1.We estimated that more than 1 in 2 uncircumcised males will experience an adverse foreskin-related medical condition over their lifetime.Wide-ranging evidence from surveys,physiological measurements,and the anatomical location of penile sensory receptors responsible for sexual sensation strongly and consistently suggested that MC has no detrimental effect on sexual function,sensitivity or pleasure.United States studies showed that early infant MC is cost saving.The evidence supporting early infant MC has further strengthened since the positive AAP and CDC reviews.CONCLUSION Affirmative MC policies are needed in Australia and New Zealand.Routine provision of accurate,unbiased education,and access in public hospitals,will maximize health and financial benefits.
