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Assessment of Meteorological Threats to the Coordinated Search and Rescue of Unmanned/Manned Aircraft
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作者 Fei YAN Chuan LI +2 位作者 Xiaoyi FU Kefeng WU Yuying LI 《Meteorological and Environmental Research》 2024年第1期27-29,37,共4页
The architecture and working principle of coordinated search and rescue system of unmanned/manned aircraft,which is composed of manned/unmanned aircraft and manned aircraft,were first introduced,and they can cooperate... The architecture and working principle of coordinated search and rescue system of unmanned/manned aircraft,which is composed of manned/unmanned aircraft and manned aircraft,were first introduced,and they can cooperate with each other to complete a search and rescue task.Secondly,a threat assessment method based on meteorological data was proposed,and potential meteorological threats,such as storms and rainfall,can be predicted by collecting and analyzing meteorological data.Finally,an experiment was carried out to evaluate the performance of the proposed method in different scenarios.The experimental results show that the coordinated search and rescue system of unmanned/manned aircraft can be used to effectively assess meteorological threats and provide accurate search and rescue guidance. 展开更多
关键词 Unmanned/manned aircraft Coordinated search and rescue Assessment of meteorological threats Meteorological data
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Development of a Sports Safety Promotion Framework Based on KABP Theory and 4M Management
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作者 Duoyang Hu Di Mu 《Journal of Contemporary Educational Research》 2024年第7期331-334,共4页
This study explores the significance,current research landscape,and conceptualization of sports safety promotion.Safety in sports is fundamental to youth physical activities,and an excessive focus on or neglect of saf... This study explores the significance,current research landscape,and conceptualization of sports safety promotion.Safety in sports is fundamental to youth physical activities,and an excessive focus on or neglect of safety is unwarranted.Globally,numerous countries have extensively researched sports safety promotion and implemented diverse strategies.Drawing from KABP(Knowledge,Attitude,Behavior,Practice)theory and 4M(Man,Machine,Medium,Management)management,this paper presents a conceptual framework for sports safety promotion.It integrates these theories to devise a comprehensive accident prevention model within a sports safety promotion system.The framework prioritizes enhancing students’safety literacy and underscores the practical application of safety knowledge and skills in simulated sports settings following structured safety education.It aims to enhance students’competency and proficiency in averting sports-related injuries. 展开更多
关键词 Knowledge Attitude Behavior Practice theory man Machine Medium management management Sports safety promotion Youth sports safety
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Study on the "Coexistence between Heaven and Man" Concept and the Chinese Vernacular Architecture Concept
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作者 胡慧 《Journal of Landscape Research》 2009年第11期73-76,93,共5页
On the basis of the characteristics of ancient Chinese culture,the paper discusses the formation inevitability of the 'Coexistence between Heaven and Man' building concept,the connection and the differences wi... On the basis of the characteristics of ancient Chinese culture,the paper discusses the formation inevitability of the 'Coexistence between Heaven and Man' building concept,the connection and the differences with the ecological concept of modern Chinese Vernacular Architecture.The key point is that the contemporary design should absorb the ancient Chinese culture of the 'Coexistence between Heaven and Man' building concept and the essence of Chinese Vernacular Architecture ecological concept,forming the design features of local ecology based on the environmental protection and harmonious coexistence. 展开更多
关键词 COEXISTENCE between HEAVEN and man ECOLOGICAL environment Architectural culture ECOLOGICAL CONCEPT of VERNACULAR Architecture
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《Man and nature》
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作者 杨滟珺 《印染》 北大核心 2019年第23期I0044-I0044,共1页
关键词 man and nature man and nature》
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Reading man皮瓣修复术治疗皮肤缺损的疗效观察
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作者 王鸣 严炜 +3 位作者 汤由之 朱喆辰 史京萍 马秀云 《临床皮肤科杂志》 CAS CSCD 北大核心 2024年第9期557-561,共5页
目的:探讨Reading man皮瓣修复术在全身不同部位皮肤缺损及其预防瘢痕增生的临床疗效。方法:回顾性分析2021年1月—2023年10月该科应用Reading man皮瓣修复术治疗全身不同部位皮肤软组织缺损的20例患者临床资料。结果:所有患者皮瓣均成... 目的:探讨Reading man皮瓣修复术在全身不同部位皮肤缺损及其预防瘢痕增生的临床疗效。方法:回顾性分析2021年1月—2023年10月该科应用Reading man皮瓣修复术治疗全身不同部位皮肤软组织缺损的20例患者临床资料。结果:所有患者皮瓣均成活。术后随访时间为3~24个月,所有皮瓣血运良好,色泽红润,质地柔软,外观适宜,局部皮肤张力较低,手术切口瘢痕增生不明显,切口周围软组织无明显形变,周围关节活动度正常。所有患者均恢复良好,疗效满意。结论:Reading man皮瓣特有的设计可以很好地解决全身多部位较大的皮肤软组织缺损,尤其在术中可以最大限度地减少正常皮肤的切除,术后外观、功能及皮肤瘢痕增生均有较满意的表现,且不存在自体皮移植带来的供皮区损伤。 展开更多
关键词 Reading man皮瓣 皮瓣血运 张力 瘢痕增生
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Protecting Our Common Homestead:A Comparative Study on Attitudes Towards Man and Nature in Chinese and the Western Culture
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作者 陈莹 《海外英语》 2010年第11X期395-397,共3页
In light of increasingly worsening environmental pollution,this paper,through comparing different attitudes towards the relationship between man and nature in Chinese and the western culture,points out rebuilding a ha... In light of increasingly worsening environmental pollution,this paper,through comparing different attitudes towards the relationship between man and nature in Chinese and the western culture,points out rebuilding a harmonious relationship between man and nature is the common object of human being,and appeals to people to shake off man-centered thinking pattern,and to treat nature as our friend. 展开更多
关键词 relationship man and NATURE protecting HARMONY
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Individual Identity and Collective Identity in Ralph Ellison's Invisible Man
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作者 韩秀 《海外英语》 2015年第8期153-155,共3页
Ralph Ellison’s Invisible Man deals with the identity issue fundamental for black Americans to reestablish themselves in the white-dominated society.While the narrator suffers from identity crisis in multiples ways,t... Ralph Ellison’s Invisible Man deals with the identity issue fundamental for black Americans to reestablish themselves in the white-dominated society.While the narrator suffers from identity crisis in multiples ways,the paper focuses on the conflict between his need to build a distinctive individual identity with the prerequisite to surrender to collective identity in order to blend in the group. 展开更多
关键词 INVISIBLE man INDIVIDUAL IDENTITY COLLECTIVE IDENTITY
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Relationship between chromatin organization, mRNAs profile and human male gamete quality 被引量:11
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作者 Isabelle Galeraud-Denis Sophie Lambard Serge Carreau 《Asian Journal of Andrology》 SCIE CAS CSCD 2007年第5期587-592,共6页
Spermiogenesis is a complex process leading to the formation of motile spermatozoa characterized by a highly stable chromatin compaction that transfers the paternal genome into the oocyte. It is commonly held that the... Spermiogenesis is a complex process leading to the formation of motile spermatozoa characterized by a highly stable chromatin compaction that transfers the paternal genome into the oocyte. It is commonly held that these haploid cells are devoid of transcriptional and translational activities and that the transcripts represent remnants of stored mRNAs. Recently, the chromatin organization of mature spermatozoa has been revisited as a double nucleoprotamine-nucleohistone structure possessing less-condensed regions sensitive to nuclease activity, which could be implicated in the expression of genes involved in the early embryo development. The existence of a complex population of mRNAs in human sperm is well-documented, but their role is not yet elucidated. Evidence for a latent transcriptional capacity and/or a potential de novo translation in mature spermatozoa from fertile men are essential for understanding the last steps of sperm maturation, such as capacitation and acrosome reaction. As such, we have documented the relationship between sperm quality and the distribution of sperm RNAs by showing divergent levels of transcripts encoding for proteins involved in either nuclear condensation (protamines 1 and 2) or in capacitation (eNOS and nNOS, c-myc) or in motility and sperm survival (aromatase) between low and high motile sperm issued from the same sample. Therefore, analyzing the profile of mRNAs could be helpful either as a diagnostic tool for evaluating male fertility after spermatogenesis or for prognosis use for fertilization. 展开更多
关键词 CHROMATIN fertility gamete quality man SPERMATOZOA TRANSCRIPTS
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大口黑鲈弹状病毒Taq Man荧光定量PCR检测方法的建立及初步应用 被引量:1
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作者 孔明慧 梁红茹 +5 位作者 李宁求 林强 牛银杰 罗霞 马宝福 付小哲 《中国预防兽医学报》 CAS CSCD 北大核心 2024年第4期379-384,共6页
为建立快速、敏感的大口黑鲈弹状病毒(MSRV)流行株Taq Man荧光定量PCR (qPCR)方法,本研究采用NCBI分析MSRV流行株N基因的保守序列,设计特异性引物和Taq Man探针,以构建的并经PCR和测序鉴定正确的重组质粒标准品p MD18-MSRV-N为模板,通... 为建立快速、敏感的大口黑鲈弹状病毒(MSRV)流行株Taq Man荧光定量PCR (qPCR)方法,本研究采用NCBI分析MSRV流行株N基因的保守序列,设计特异性引物和Taq Man探针,以构建的并经PCR和测序鉴定正确的重组质粒标准品p MD18-MSRV-N为模板,通过优化各反应条件初步建立MSRV的Taq Man q PCR检测方法。结果显示,建立的Taq Man q PCR方法标准曲线的R2为0.9906,质粒标准品在6.79×10^(8)拷贝/μL~6.79×10^(2)拷贝/μL范围内与Ct值均存在较好的线性关系。采用该方法检测MSRV及相关病毒,评估该方法的特异性,结果显示,该方法可以特异性检测MSRV,而草鱼出血病病毒、神经坏死病毒、鲤春病毒血症病毒、大口黑鲈双RNA病毒、鳜蛙虹彩病毒、传染性脾肾坏死病毒、石斑鱼虹彩病毒、大口黑鲈蛙虹彩病毒等8种常见鱼类病毒检测结果均为阴性;采用建立的方法检测不同浓度的质粒标准品(6.79×10^(8)拷贝/μL~6.79×10^(1)拷贝/μL),评估该方法的敏感性,结果显示,该方法的检测限为6.79×10^(2)拷贝/μL,比文献中的常规PCR方法敏感1 000倍;以不同批次或同一批次提取的不同浓度的质粒标准品作为模板,分别利用该方法检测,评估该方法的重复性,结果显示,该方法批内和批间重复性试验的变异系数均小于2%。采用该方法和文献中的常规PCR同时检测71份临床发病的大口黑鲈、鳜鱼、鲮鱼组织样品,结果显示阳性率为16.9%(12/71),阴性率为83.1%(59/71),且阳性样品均是从发病大口黑鲈的组织样品中检测到,而发病的鳜鱼、鲮鱼组织样品中均未检测到该病毒;常规PCR方法的阳性率为9.9%(7/71),阴性率为90.1%(64/71),二者的总符合率为93.0%。本研究建立的MSRV Taq Man q PCR方法特异性强、敏感性高、重复性和准确性均较好,可用于大口黑鲈临床样品的检测,为MSRV的快速检测提供了可行的技术手段。 展开更多
关键词 大口黑鲈弹状病毒 Taq man荧光定量PCR N基因
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Design and research on a variable ballast system for deep-sea manned submersibles 被引量:7
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作者 邱中梁 《Journal of Marine Science and Application》 2008年第4期255-260,共6页
Variable ballast systems are necessary for manned submersibles to adjust their buoyancy.In this paper,the design of a variable ballast system for a manned submersible is described.The variable ballast system uses a su... Variable ballast systems are necessary for manned submersibles to adjust their buoyancy.In this paper,the design of a variable ballast system for a manned submersible is described.The variable ballast system uses a super high pressure hydraulic seawater system.A super high pressure seawater pump and a deep-sea brushless DC motor are used to pump seawater into or from the variable ballast tank,increasing or decreasing the weight of the manned submersible.A magnetostrictive linear displacement transducer can detect the seawater level in the variable ballast tank.Some seawater valves are used to control pumping direction and control on-off states.The design and testing procedure for the valves is described.Finally,the future development of variable ballast systems and seawater hydraulic systems is projected. 展开更多
关键词 DEEP-SEA manned submersible variable ballast system WEIGHT seawater pump seawater hydraulic system
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Formation and adjustment of manned/unmanned combat aerial vehicle cooperative engagement system 被引量:18
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作者 ZHONG Yun YAO Peiyang +1 位作者 ZHANG Jieyong WAN Lujun 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2018年第4期756-767,共12页
Manned combat aerial vehicles (MCAVs), and un-manned combat aerial vehicles (UCAVs) together form a cooper-ative engagement system to carry out operational mission, whichwill be a new air engagement style in the n... Manned combat aerial vehicles (MCAVs), and un-manned combat aerial vehicles (UCAVs) together form a cooper-ative engagement system to carry out operational mission, whichwill be a new air engagement style in the near future. On the basisof analyzing the structure of the MCAV/UCAV cooperative engage-ment system, this paper divides the unique system into three hi-erarchical levels, respectively, i.e., mission level, task-cluster leveland task level. To solve the formation and adjustment problem ofthe latter two levels, three corresponding mathematical modelsare established. To solve these models, three algorithms calledquantum artificial bee colony (QABC) algorithm, greedy strategy(GS) and two-stage greedy strategy (TSGS) are proposed. Finally,a series of simulation experiments are designed to verify the effec-tiveness and superiority of the proposed algorithms. 展开更多
关键词 manned combat aerial vehicle (MCAV) unmannedcombat aerial vehicle (UCAV) cooperative engagement system quantum artificial bee colony (QABC) greedy strategy (GS) two-stage greedy strategy (TSGS)
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Target Deployment and Retrieval Using JIAOLONG Manned Submersible in the Depth of 6600 m in Mariana Trench 被引量:3
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作者 GAO Xiang DING Kang +4 位作者 REN Yu-gang FU Wen-tao DING Zhong-jun ZHAO Sheng-ya LIU Bao-hua 《China Ocean Engineering》 SCIE EI CSCD 2017年第5期618-623,共6页
China's 7000 m manned submersible JIAOLONG carried out an exploration cruise at the Mariana Trench from June to July 2016. The submersible completed nine manned dives on the north and south area of the Mariana Trench... China's 7000 m manned submersible JIAOLONG carried out an exploration cruise at the Mariana Trench from June to July 2016. The submersible completed nine manned dives on the north and south area of the Mariana Trench from the depth of 5500 to 6700 m, to investigate the geological, biological and chemical characteristics in the hadal area. During the cruise, JIAOLONG deployed a gas-tight serial sampler to collect the water near the sea bottom regularly. Five days later, the sub located the sampler in another dive and retrieved it successfully from the same location, which is the first time that scientists and engineers finished the high accuracy in-situ deployment and retrieval using a manned submersible with Ultra-Short Base Line (USBL) positioning system at the depth more than 6600 m. In this task, we used not only the USBL system of the manned submersible but also a compound strategy, including five position marks, the sea floor terrain, the depth contour, and the heading of the sub. This paper introduces the compound strategy of the target deployment and retrieval with the practical diving experience of JIAOLONG, and provides a promising technique for other underwater vehicles such as manned submersible or Remote Operated Vehicle (ROV) under similar conditions. 展开更多
关键词 target deployment and retrieval manned submersible USBL Mariana Trench
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Dynamic Modeling and Investigation of Maneuver Characteristics of A Deep-Sea Manned Submarine Vehicle 被引量:3
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作者 谢俊元 须文波 +2 位作者 张华 徐鹏飞 崔维成 《China Ocean Engineering》 SCIE EI 2009年第3期505-516,共12页
A deep-sea Manned Submarine Vehicle (MSV) is usually required to move at a low forward speed and a low rotational speed when it executes investigation tasks. In this condition, the motion is in large drift angles, a... A deep-sea Manned Submarine Vehicle (MSV) is usually required to move at a low forward speed and a low rotational speed when it executes investigation tasks. In this condition, the motion is in large drift angles, and the maneuverability hydrodynamic forces cannot be expressed properly in the conventional mathematical model of submersible motion. In this paper, firstly, a general equation of MSV with six-freedom motion is presented, and the numerical simulation of descent/ascent motion and helix motion is conducted to reveal the general maneuver characteristics of MSV. Secondly, according to the data of captive model tests of large drift angles of MSV, the regression analysis of position hydrodynamic forces and rotation hydrodynamic forces is carried out, and the results of regression analysis of maneuverability hydrody- namic characteristics are analyzed to reveal the special maneuver characteristics. Thirdly, a special new mathematical model of MSV with the whole range of drift angles motion is presented, which can be used to predict hydrodynamic performance of motion in the 0° - 180° range of drift angles. The results are applied to the design of maneuverability hydrodynamic forces, development of control system and simulator of a practical MSV. 展开更多
关键词 manned Submarine Vehicle MSV) dynamic and kinematic modeling large drift angle numerical simulation
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Multi‑Crack Interaction and Influence on the Spherical Pressure Hull for a Deep‑Sea Manned Submersible 被引量:3
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作者 Rujun Li Yongmei Zhu +1 位作者 Wenjing Fang Baoji Yin 《Journal of Marine Science and Application》 CSCD 2021年第3期491-503,共13页
This study investigates the interaction and influence of surface cracks on the spherical pressure hull of a deep-sea manned submersible.The finite element model of the spherical hull is established,and a semi-elliptic... This study investigates the interaction and influence of surface cracks on the spherical pressure hull of a deep-sea manned submersible.The finite element model of the spherical hull is established,and a semi-elliptical surface crack is inserted in the welding toe of the spherical hull as the main crack.Considering the combined effect of external uniform pressure and welding residual stress at the weld toe,the stress intensity factor(SIF)is obtained based on the M-integral method.Inserting disturbing cracks at different positions on the spherical hull surface,the interaction and influence between multi-cracks are revealed by numerical calculation.The results show that the existence of the disturbing crack has a great influence on the stress intensity factor of the main crack,and the influence is different with the different location of disturbing crack.The study of the interaction of multiple cracks under different interference factors and the influence of disturbing cracks on the main crack can provide some reference for future engineering applications. 展开更多
关键词 Spherical pressure hull Surface cracks Multi-crack interaction Stress intensity factor Welding residual stress manned submersible Finite element model
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The Enlightenment of the Old Man and the Sea 被引量:1
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作者 李哲 《海外英语》 2010年第5X期228-228,共1页
The Old Man and the Sea tells a tough and frustrated experience of an old fisherman.The theme is a praise of heroism.The enlightenment of the work is that "man is not made for defeat...[a] man can be destroyed bu... The Old Man and the Sea tells a tough and frustrated experience of an old fisherman.The theme is a praise of heroism.The enlightenment of the work is that "man is not made for defeat...[a] man can be destroyed but not defeated." 展开更多
关键词 ENLIGHTENMENT the OLD man NEVER GIVE up
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Study on the Overload and Dwell-Fatigue Property of Titanium Alloy in Manned Deep Submersible 被引量:2
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作者 WANG Ke WU Li +1 位作者 LI Yong-zheng SUN Xiao-peng 《China Ocean Engineering》 SCIE EI CSCD 2020年第5期738-745,共8页
With the rapid development of ocean technology, the deep-sea manned submersible is regarded as a high-tech equipment for the exploration and exploitation of ocean resources. The safety of manned cabin has a decisive e... With the rapid development of ocean technology, the deep-sea manned submersible is regarded as a high-tech equipment for the exploration and exploitation of ocean resources. The safety of manned cabin has a decisive effect on the whole system. Ti-6 Al-4 V with the superior strength-to-weight ratio and corrosion resistance has been used for the manned cabin. The manned cabin experiences loading spectrum with different maximum stresses and different dwell time during their service life. The load sequence effects on dwell fatigue crack growth behavior of Ti-6 Al-4 V under different dwell time are investigated experimentally in this paper. The experimental results show that the crack tip plastic zone is enlarged by the dwell time and the overload retardation zone increases with dwell time under the same overload rate. A dwell fatigue crack growth model is proposed by modifying the crack tip plastic zone under the loading history with combinations of the single overload and dwell time factors are included in the modified model. Based on the experimental data, the overload retardation zone and the crack growth rates of Ti-6 Al-4 V are predicted by the modified model. A reasonable model for the load sequence effect on the dwell fatigue crack growth rates of Ti-6 Al-4 V is verified. 展开更多
关键词 manned cabin dwell fatigue crack growth rate TI-6AL-4V load sequence effect
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Solution of Dirac equation for Eckart potential and trigonometric Manning Rosen potential using asymptotic iteration method 被引量:1
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作者 resita arum sari a suparmi c cari 《Chinese Physics B》 SCIE EI CAS CSCD 2016年第1期428-435,共8页
The Dirac equation for Eckart potential and trigonometric Manning Rosen potential with exact spin symmetry is obtained using an asymptotic iteration method. The combination of the two potentials is substituted into th... The Dirac equation for Eckart potential and trigonometric Manning Rosen potential with exact spin symmetry is obtained using an asymptotic iteration method. The combination of the two potentials is substituted into the Dirac equation, then the variables are separated into radial and angular parts. The Dirac equation is solved by using an asymptotic iteration method that can reduce the second order differential equation into a differential equation with substitution variables of hypergeometry type. The relativistic energy is calculated using Matlab 2011. This study is limited to the case of spin symmetry. With the asymptotic iteration method, the energy spectra of the relativistic equations and equations of orbital quantum number l can be obtained, where both are interrelated between quantum numbers. The energy spectrum is also numerically solved using the Matlab software, where the increase in the radial quantum number nr causes the energy to decrease. The radial part and the angular part of the wave function are defined as hypergeometry functions and visualized with Matlab 2011. The results show that the disturbance of a combination of the Eckart potential and trigonometric Manning Rosen potential can change the radial part and the angular part of the wave function. 展开更多
关键词 Dirac equation Eckart potential trigonometric manning Rosen potential spin symmetric
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A True Winner On Hemingway's The Old Man And The Sea 被引量:3
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作者 陆连枝 《百色学院学报》 1999年第2期73-76,共4页
Ernest Hemingway, a famous American writer, is considered as one of tbe most impor-tant and Outstanding wriers of the 20th century in the world. This essay is to deal mainly with tbe writ-ing techniques OfThe Old Man ... Ernest Hemingway, a famous American writer, is considered as one of tbe most impor-tant and Outstanding wriers of the 20th century in the world. This essay is to deal mainly with tbe writ-ing techniques OfThe Old Man And the Sea as well as the character about the main hero, aiming to helpthe readers get a better understanding of the masterpiece by Hemingway- 展开更多
关键词 HEMINGWAY the OLD man and The SEA TRUE WINNER
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Tentative Interpretation of The Old Man and the Sea 被引量:1
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作者 苏索才 《外语教学》 CSSCI 北大核心 1994年第4期68-71,84,共5页
本文试图解析《老人与海》中老人和海的形象,认为小说中的老人是勇气、智慧和尊严的象征,是一个圣者,同时又具有常人的弱点;并认为老人对大海未知领域的探求是海明威自身的写照。小说中的大海不仅是一种神秘、巨大、与人为敌的自然... 本文试图解析《老人与海》中老人和海的形象,认为小说中的老人是勇气、智慧和尊严的象征,是一个圣者,同时又具有常人的弱点;并认为老人对大海未知领域的探求是海明威自身的写照。小说中的大海不仅是一种神秘、巨大、与人为敌的自然力量,也是一种和善、能与人类相容的女人、兄弟和朋友。同时还认为老人与大海的结合一方面是老人一生与海相搏以达到相容的结果,同时也是他们自身和善、任性的产物。通过塑造两个形象,海明威在人物、小说背景安排和主题上实现了突破。在晚年,他更关心日常生活中的常人和他们所处的环境,对他们的勇气、智慧、特别是尊严给予高度赞扬。老人是海明威一生创作的这一系列"英雄"群像的一个新的里程碑,是其一生乐观主义精神追求的最终结果。 展开更多
关键词 老人 大海 未知领域 Tentative Interpretation of The Old man and the Sea 小说 海明威 自然力量 日常生活 新的里程碑 群像 雕塑像
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鸡毒支原体 Taq Man实时荧光定量PCR检测方法的建立
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作者 孙宇 苗立中 +6 位作者 程立坤 赵家磊 赵修报 沈志强 王敬茹 李书光 余燕 《动物医学进展》 北大核心 2024年第4期64-68,共5页
为快速检测鸡毒支原体,根据鸡毒支原体的保守基因设计特异性引物和Taq Man探针,建立鸡毒支原体Taq Man实时荧光定量PCR方法并分析鸡毒支原体培养过程中颜色单位改变法(color change unit,CCU)和荧光定量PCR检测的相关性。结果显示,标准... 为快速检测鸡毒支原体,根据鸡毒支原体的保守基因设计特异性引物和Taq Man探针,建立鸡毒支原体Taq Man实时荧光定量PCR方法并分析鸡毒支原体培养过程中颜色单位改变法(color change unit,CCU)和荧光定量PCR检测的相关性。结果显示,标准曲线y=-3.646x+44.208,相关系数R^(2)=0.998,最低检测限度为1 copy/μL;与其他菌株等均无交叉反应;组内和组间变异系数均小于3%,表明该方法具有良好的稳定性和可重复性。用建立的实时荧光定量PCR检测方法对26份临床样品进行检测,该方法较普通PCR方法检出率更高。建立的荧光定量PCR与CCU显示鸡毒支原体在对数生长期时,两者具有一定的对应关系。表明建立了鸡毒支原体Taq Man探针实时荧光定量PCR检测方法,可实现对鸡毒支原体的快速检测,为鸡毒支原体感染的防控提供技术基础。 展开更多
关键词 鸡毒支原体 Taq man探针 荧光定量PCR
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