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Glacier area change and its impact on runoff in the Manas River Basin,Northwest China from 2000 to 2020
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作者 WANG Tongxia CHEN Fulong +5 位作者 LONG Aihua ZHANG Zhengyong HE Chaofei LYU Tingbo LIU Bo HUANG Yanhao 《Journal of Arid Land》 SCIE CSCD 2024年第7期877-894,共18页
Understanding the distribution and dynamics of glaciers is of great significance to the management and allocation of regional water resources and socio-economic development in arid regions of Northwest China.In this s... Understanding the distribution and dynamics of glaciers is of great significance to the management and allocation of regional water resources and socio-economic development in arid regions of Northwest China.In this study,based on 36 Landsat images,we extracted the glacier boundaries in the Manas River Basin,Northwest China from 2000 to 2020 using eCognition combined with band operation,GIS(geographic information system)spatial overlay techniques,and manual visual interpretation.We further analyzed the distribution and variation characteristics of glacier area,and simulated glacial runoff using a distributed degree-day model to explore the regulation of runoff recharge.The results showed that glacier area in the Manas River Basin as a whole showed a downward trend over the past 21 a,with a decrease of 10.86%and an average change rate of–0.54%/a.With the increase in glacier scale,the number of smaller glaciers decreased exponentially,and the number and area of larger glaciers were relatively stable.Glacier area showed a normal distribution trend of increasing first and then decreasing with elevation.About 97.92%of glaciers were distributed at 3700–4800 m,and 48.11%of glaciers were observed on the northern and northeastern slopes.The retreat rate of glaciers was the fastest(68.82%)at elevations below 3800 m.There was a clear rise in elevation at the end of glaciers.Glaciers at different slope directions showed a rapid melting trend from the western slope to the southern slope then to the northern slope.Glacial runoff in the basin showed a fluctuating upward trend in the past 21 a,with an increase rate of 0.03×1010^(8) m^(3)/a.The average annual glacial runoff was 4.80×10^(8) m^(3),of which 33.31%was distributed in the ablation season(June–September).The average annual contribution rate of glacial meltwater to river runoff was 35.40%,and glacial runoff accounted for 45.37%of the total runoff during the ablation season.In addition,precipitation and glacial runoff had complementary regulation patterns for river runoff.The findings can provide a scientific basis for water resource management in the Manas River Basin and other similar arid inland river basins. 展开更多
关键词 glacier area glacial runoff climate change glacier boundary extraction distributed degree-day model manas River Basin
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Surface runoff processes and sustainable utilization of water resources in Manas River Basin, Xinjiang, China 被引量:18
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作者 HongBo LING HaiLiang XU +1 位作者 JinYi FU XinHua LIU 《Journal of Arid Land》 SCIE 2012年第3期271-280,共10页
Water is the important resource to guarantee the existence and development of oases in arid areas. To improve the utilization efficiency of water resources in Manas River Basin, this paper investigated the trends and ... Water is the important resource to guarantee the existence and development of oases in arid areas. To improve the utilization efficiency of water resources in Manas River Basin, this paper investigated the trends and periods of runoff based on the runoff and climate data for the past 50 years. Subsequently, with the socioeconomic and water resources data, we studied a comprehensive evaluation on the water security in this area. The results indicated that the stream flows in the three hydrological stations of Hongshanzui, Kensiwat and Bajiahu have sig- nificantly increased and undergone abrupt changes, with periods of 18 and 20 years. According to assessment, water security in the Manas River Basin was at an unsafe level in 2008. In criterion layer, the ecological security index and the index of supply-demand situation are both at the relatively secure level; the quantity index and so- cioeconomic index of water resources are at the unsafe level and basic security level, respectively. Therefore, in order to achieve sustainable economic and social development within the Manas River Basin, it is vital to take a series of effective measures to improve the status of water security. 展开更多
关键词 surface runoff processes period and trend sustainable utilization water resources the manas River Basin fuzzycomprehensive evaluation
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Spatial distribution of soil moisture,salinity and organic matter in Manas River watershed,Xinjiang,China 被引量:9
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作者 Jilili ABUDUWAILI Yang TANG +2 位作者 Mireban ABULIMITI DongWei LIU Long MA 《Journal of Arid Land》 SCIE 2012年第4期441-449,共9页
With the classical statistical and geostatistical methods, the study of the spatial distribution and its in- fluence factors of soil water, salinity and organic matter was carried out for 0-70 cm soil layers in Manas ... With the classical statistical and geostatistical methods, the study of the spatial distribution and its in- fluence factors of soil water, salinity and organic matter was carried out for 0-70 cm soil layers in Manas River watershed. The results showed that the soil moisture data from all soil layers exhibited a normal distribution, with average values of 14.08%-21.55%. Geostatistical analysis revealed that the content of soil moisture had a moder- ate spatial autocorrelation with the ratios of nugget/sill ranging from 0.500 to 0.718, which implies that the spatial pattern of soil moisture is influenced by the combined effects of structural factors and random factors. Remarkable spatial distributions with stripped and mottled features were found for soil moisture in all different soil layers. The landform and crop planting had a relatively big influence on the spatial distribution of soil moisture; total soil salinity was high in east but low in west, and non-salinized soil and lightly salinized soil appeared at the northwest and southwest of the study area. Under the effect of reservoir leakage, the heavily salinized soils are widely distributed in the middle of the study area. The areas of the non-salinized and lightly salinized soils decreased gradually with soil depth increment, which is contrary to the case for saline soils that reached a maximum of 245.67 km2 at the layer of 50-70 cm. The types of soil salinization in Manas River watershed were classified into four classes: the sulfate, chloride-sulfate, sulfate-chloride and chloride. The sulfate salinized soil is most widely distributed in the surface layer. The areas of chloride-sulfate, sulfate-chloride, and chloride salinized soils increased gradually along with the increment of soil depth; the variation range of the average values of soil organic matter content was be- tween 7.48%-11.33%. The ratios of nugget/sill reduced gradually from 0.698 to 0.299 with soil depth increment, which shows that the content of soil organic matter has a moderate spatial autocorrelation. The soil organic matter in all soil layers met normal distribution after logarithmic transformation. The spatial distribution patterns of soil or- ganic matter and soil moisture were similar; the areas with high organic matter contents were mainly distributed in the south of the study area, with the lowest contents in the middle. 展开更多
关键词 soil water and salt organic matter spatial distribution manas River watershed
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An oases dynamics model and its application in the Manas oasis 被引量:5
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作者 CHEN Zhengjiang, SHI Wenzhong, HUI Yanghe(1. Dept. of Urban and Resource, Northwest University, Xi’an 710069, China 2. Dept. of LandSurvey and Geo-information, The Hong Kong Polytechnic University, Hong Kong, China) 《Journal of Geographical Sciences》 SCIE CSCD 2002年第3期348-356,共9页
This study applies theories of systems and system dynamics to ecological economic systems in the oasis environment, with the intention of finding the basic characteristics of the oasis environment and its feedback str... This study applies theories of systems and system dynamics to ecological economic systems in the oasis environment, with the intention of finding the basic characteristics of the oasis environment and its feedback structures. This study explores the inner mechanisms of economic development in the oasis environment and its relationship to policy variables. This model is applied to economic development in the Manas oasis of the Xinjiang Uygur Autonomous Region of China. System dynamics method starts from studying feedback structure within the system study and then searches for the best solution through identifying the right “policy point” and through model simulation in the computer. It embodies the cream of “systems cybernetics theory”. This study is justified because the nature of the oasis system and the simulation results are scientifically sound and practically feasible. 展开更多
关键词 OASIS dynamic modeling manas oasis
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Tectonic geomorphological characteristics for evolution of the Manas Lake 被引量:1
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作者 Yao, YongHui Li, HuiGuo 《Journal of Arid Land》 SCIE 2010年第3期167-173,共7页
关键词 the manas Lake the Junggar Basin wandering lake lacustrine evolution lake inflow Xinjiang
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The Effect of Extreme Climatic Events on Extreme Runoff in the Past 50 Years in the Manas River Basin, Xinjiang, China 被引量:1
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作者 Xiangling Tang Xin Lv 《American Journal of Climate Change》 2016年第1期15-26,共12页
To analyze extreme climatic change features and effects on runoff in the Manas River basin, Xinjiang, data were collected including daily mean temperature, daily highest and lowest temperatures, and daily precipitatio... To analyze extreme climatic change features and effects on runoff in the Manas River basin, Xinjiang, data were collected including daily mean temperature, daily highest and lowest temperatures, and daily precipitation from six meteorological stations in the Manas River basin as well as daily runoff data from the Kensiwate hydrologic stations during 1960-2010. By adopting the threshold value of extreme climatic events defined by ET ALDDMI and with the aid of nonparametric statistical tests, Pearson III methods, and others, the effect of extreme climatic events on extreme runoff in the past 50 years in the Manas River basin, Xinjiang, was analyzed. The results showed that in the past 50 years, 1) extreme warming events (annual extreme maximum temperature, warm-day and warm-night index) have risen significantly (P < 0.05). Among these the warm-day and warm-night indices decreased abruptly in 2001 and 1996, respectively. With respect to extreme cold events (annual extreme minimum temperature, cold-day and cold-night indices), the extreme minimum temperature was high after 1976, and the cold-day index weakened significantly, similar to the cold-night index. 2) Except for the continuous drought days (CDD), the other five indices of extreme precipitation events appeared to trend upward, with an abrupt change around 1993. 3) Flood events in 1990, mostly in summer, accounted for 42.9% of the total number of floods since 1960. Floods increased mainly because extremely high summer temperatures increased snowmelt, increasing inflow to the rivers, which combined with more precipitation to cause the increase in summer peak flood discharge. 展开更多
关键词 manas River Basin Extreme Climate Events Extreme Runoff INFLUENCE
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PROTECTION AND RATIONAL USE OF THE LAND RESOURCE IN THE MANAS RIVER BASIN OF XINJIANG
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作者 Yuan Guoying Xinjang Institute of Environmental Research, Urumqi, 830011 《干旱区资源与环境》 CSCD 1993年第Z1期466-467,共2页
The Manas River valley, situated in the middle part of northern slope of TianshanMountains, in a geographcal position of N43°20′--45°56′, E84°57′--86°43′,composed of Manas and Shawan counties, ... The Manas River valley, situated in the middle part of northern slope of TianshanMountains, in a geographcal position of N43°20′--45°56′, E84°57′--86°43′,composed of Manas and Shawan counties, and the 8th Agricaltural Reclamation Division,has a total area of 24328Km^2. This area belongs to the arid regions of China with a great distance to the sea. For in- 展开更多
关键词 manas River BASIN Ecoagriculture PROTECTION for Environment and RESOURCES
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The oasis expansion and eco-environment change over the last 50 years in Manas River Valley, Xinjiang 被引量:24
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作者 CHENG Weiming ZHOU Chenghu LIU Haijiang ZHANG Yang JIANG Yan ZHANG Yichi YAO Yonghui 《Science China Earth Sciences》 SCIE EI CAS 2006年第2期163-175,共13页
The oasis expansion and economic development of the Manas River Valley is one of the most successful examples in Xinjiang. In this paper, the oasis spatial distribution pattern and dynamic change were examined using i... The oasis expansion and economic development of the Manas River Valley is one of the most successful examples in Xinjiang. In this paper, the oasis spatial distribution pattern and dynamic change were examined using imageries of Landsat MSS, TM and ETM, land use and topographic maps in six different periods (such as 1949, 1962, 1976, 1989, 1999 and 2001) over the last 50 years in the valley. The oasis expansion process could be divided into two stages according to the annual rate of oasis area increase, the fast oasization stage (from 1949 to 1976) when the oasis area ex-panded from 156.385 km2 in 1949 to 3,639.491 km2 in 1976 because of rapidly increased population and quickly enlarged farmland area; and the urbanization stage (from 1976 to 2001) when the ex-pansion ratio of farmland slowed down, and the urbanization process quickened, the total oasis area reached 5042.440 km2 in 2001. With the continuous expansion of oasis and farmland, a large quantity of river water was drawn into irrigation ditch and plain lakes, so only a small quantity of river water could flow into lakes, as a result, the Manas Lake dried up, salization and basification problems happened in the low-lying oasis region. The natural swamp around the lake was shrunk greatly and biodiversity decreased significantly. The driving force analysis reveals that human activities, such as reclamation and population growth, played a major role in the oasis expansion and ecological dete-rioration in the Manas River Valley. So further efforts should be made to improve the efficiency of water resource utility and adjust layout of the regional agricultural and animal husbandry to keep the sustainable development of oasis economic belt of the northern slope of Tianshan Mts. 展开更多
关键词 manas River Valley oasization urbanization SALINIZATION and basification remote sensing (RS).
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Transformation of surface water and groundwater and water balance in the agricultural irrigation area of the Manas River Basin,China 被引量:9
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作者 Yang Guang He Xinlin +2 位作者 Li Xiaolong Long Aihua Xue Lianqing 《International Journal of Agricultural and Biological Engineering》 SCIE EI CAS 2017年第4期107-118,共12页
Calculation of the water balance is very important to relieve the pressure on water resources in arid agricultural irrigation areas.This research focused on irrigation water balance calculations in the Manas River Bas... Calculation of the water balance is very important to relieve the pressure on water resources in arid agricultural irrigation areas.This research focused on irrigation water balance calculations in the Manas River Basin of the southern margin of the Junggar Basin of China,and aimed to analyze the groundwater level dynamic trend and response characteristics of the basin water cycle under water-saving irrigation measures.The surface water and groundwater coupling model of MIKE 11-Visual MODFLOW was used to simulate rainfall runoff in mountainous areas,and quantify the contribution of water balance components in the plain irrigation area.Convergence of the delayed watershed in the mountain area was obvious,and when the river runoff exceeded 200 m^(3)/s,the error in the runoff simulation was large.The water balance in the plain agricultural irrigation area was in a negative equilibrium state,and the difference between recharge and discharge was−2.65 billion m^(3).The evapotranspiration was 24.49 billion m^(3) under drip irrigation,accounting for only approximately 51%of the total discharge.The lateral discharge of the unsaturated and saturated aquifers was 15.38 billion m^(3),accounting for approximately 32%of the total discharge.The main reason for the groundwater decline in the irrigation area was closely related to the extraction of groundwater,because the amount of recharge and discharge in the natural state was approximately identical.The MIKE 11-Visual MODFLOW model produced accurate results,and the research method provided a new exploration technique to quantify the effect of water supply mode on the groundwater table.The model is suitable for the management of water resources in arid areas. 展开更多
关键词 water balance coupling model surface water and groundwater water saving irrigation manas River Basin
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Land use change characteristics affected by water saving practices in Manas River Basin,China using Landsat satellite images 被引量:5
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作者 Yang Guang Chen Dong +3 位作者 He Xinlin Long Aihua Yang Mingjie Li Xiaolong 《International Journal of Agricultural and Biological Engineering》 SCIE EI CAS 2017年第6期123-133,共11页
The characteristics and influencing factors of land use change under arid conditions were studied in the Manas River Basin in Xinjiang Region,Northwest China.Landsat satellite images acquired in 1976,1990,2000,2010 an... The characteristics and influencing factors of land use change under arid conditions were studied in the Manas River Basin in Xinjiang Region,Northwest China.Landsat satellite images acquired in 1976,1990,2000,2010 and 2015 over the study area were used as basic data.Land use change,the rate of change of land use,land use transfer and other aspects revealed the characteristics of land use change and related factors as influenced by water conditions in the basin.The results showed that:(1)Over nearly 50 years,land reclamation in the Manas River Basin resulted in the rapid expansion of an artificial oasis area,and promoted the process of‘oasis urbanization’,and accelerated the development of the river basin economy.(2)In 2000,the popularization of drip irrigation under mulch technology led to the rapid growth of cultivated land and development land in the watershed.Meanwhile,the water table declined in the desert area of the lower reaches of the river basin,and the area occupied by sparse shrub forest and grassland decreased.(3)Before popularization of water-saving technology,woodland,grassland and development land transformed to cultivated land in the amounts of 93.46 km^(2),2542.93 km^(2) and 137.53 km^(2),respectively,and woodland transformed in the amount of 189.64 km^(2).After water-saving technology was popularized,woodland,grassland and development land were transformed into cultivated land in the amounts of 567.41 km^(2),1756.2 km^(2) and 37.36 km^(2),respectively.(4)The popularization of water-saving technology made the dynamic degree of cultivated land and development land more active,and further increased landscape fragmentation and landscape heterogeneity.The level of urbanization development,the level of economic development and the dry humidity of the basin became the main factors affecting the change of land use in the basin. 展开更多
关键词 land-use change spatial characteristics influencing factor manas River Basin Landsat satellite images
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Effects of drip irrigation on components of water cycle in arid inland areas: A case study of Manas river basin in northwestern China 被引量:3
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作者 Pengfei Li Guang Yang +3 位作者 Xinlin He Fadong Li Ke Yan Zelin Wang 《International Journal of Agricultural and Biological Engineering》 SCIE EI CAS 2019年第1期132-138,共7页
Compared to either drip irrigation or mulching with plastic film,the two methods together can reduce water requirements of crops grown in arid areas by more than 30%.Such a combination deployed on a large scale(1)redu... Compared to either drip irrigation or mulching with plastic film,the two methods together can reduce water requirements of crops grown in arid areas by more than 30%.Such a combination deployed on a large scale(1)reduced the loss of soil water by 31.8%compared to that from drip irrigation alone;(2)narrowed the range of annual evapotranspiration from 1582.4-1780.3 mm,which is average for the basin,to 222.2-294.8 mm;and(3)increased the overall humidity in the central plain of the basin.However,the surrounding regions in which drip irrigation is not combined with mulching are getting more arid;thus,as a result of the water-saving technology,both oases and the desertification of the river basin are increasing at the same time.The results of the study further the understanding of the effects of drip irrigation combined with mulching on water cycles in the basin of the Manas river and suggest ways to protect the ecology and the environment of the basin. 展开更多
关键词 EVAPOTRANSPIRATION drip irrigation arid inland areas water cycle Mann-Kendall rank test MOD16 manas river basin
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Response of groundwater to the process of reservoirs group regulation and storage in Manas River Basin in Xinjiang 被引量:2
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作者 Bing Liu Guang Yang Xinlin He 《International Journal of Agricultural and Biological Engineering》 SCIE EI CAS 2020年第1期224-233,共10页
Reservoir regulation and storage is the main approach to alleviate the water pressure caused by the uneven spatial and temporal distribution of surface water resources in arid areas of northwest China.While the regula... Reservoir regulation and storage is the main approach to alleviate the water pressure caused by the uneven spatial and temporal distribution of surface water resources in arid areas of northwest China.While the regulation and storage of the reservoir affect the conditions of recharge and discharge of groundwater,the process of regional surface-groundwater transformation tends to be complicated.The Manas River basin that lies on the northern slope of Tianshan Mountain in Northwest China was taken in this study as a case.A numerical model of groundwater in basin plain area was established,and the influence of reservoir regulation on groundwater level was studied.The results showed that the total recharge of groundwater in the study area was 75.539 million m3,the total discharge of groundwater was 82.66 million m3,and the groundwater in the study area was in a negative equilibrium state,with a difference of-69.27 million m3.The water balance method was used to verify the comparison.The total recharge of groundwater was 74.34 million m3,the total discharge of groundwater was 80.726 million m3,and the calculation result of the numerical simulation of the supplementary displacement was 63.82 million m3,basically consistent with the calculation result of the water balance method.The reservoir storage method has obvious changes to the groundwater level around the reservoir.The simulation results of groundwater numerical model showed that when the reservoir was in normal operation,the leakage of the reservoir was 27.35 million m3;when the reservoir was operated at low water level,the leakage of the reservoir was the smallest,13.47 million m3.The reservoir has the largest amount of leakage of 41.85 million m3 when operated at water storage level.When the reservoir was operated at the lowest water level,the groundwater level around the reservoir was declining compare to the normal operating water level.The maximum drop of the groundwater observation well was 2.1 m,and the maximum monthly average was 0.99 m.When the reservoir was operating at the normal water storage level,the water level of the groundwater around the reservoir has increased compare to the normal operating water level.The maximum increase of the groundwater observation well was 1.5 m,and the maximum monthly average increase was 0.78 m.The influence of the reservoir on the groundwater level was 2000 m upstream and 12000 m downstream.The research conclusions can provide a scientific reference for the development,utilization and management of regional groundwater. 展开更多
关键词 manas River Basin water balance numerical simulation reservoir regulating GROUNDWATER
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Land use/cover and landscape pattern changes in Manas River Basin based on remote sensing
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作者 Xiaolong Li Xinlin He +5 位作者 Guang Yang Hongguang Liu Aihua Long Fulong Chen Bing Liu Xinchen Gu 《International Journal of Agricultural and Biological Engineering》 SCIE EI CAS 2020年第5期141-152,共12页
Large-scale water and soil development in inland river basins in arid areas has made changes in landscape composition and structure,threatening the ecological balance.In order to study the trend of land use/cover and ... Large-scale water and soil development in inland river basins in arid areas has made changes in landscape composition and structure,threatening the ecological balance.In order to study the trend of land use/cover and landscape pattern change and its relationship with water resources utilization in Manas River Basin,the land-use data of five periods in 1976,1990,2000,2010 and 2015 were analyzed.The results showed that:(1)During 1976-2015,farmland and construction land continuously increased,forest land and grassland continuously decreased,the water area initially reduced and then increased,the area of saline-alkali land initially increased and then reduced,the overall trend of unused land and the sandy area was decreasing and the area of different time periods is floating.The areas of land-use types either increased or decreased.This indicates that the landscape pattern of the basin changes dramatically,and human activities are the main reasons for this phenomenon;(2)The oasis area increased from 3480.2 km2 in 1976 to 7982.0 km2 in 2015,with an obvious increasing trend.The oasis area clearly increased during 1976-1990,the growth rate was 40.6%,the growth rate of the oasis area was 129.4%.In the last 40 years,the degree of desertification fluctuated and decreased.The increase of the oasis area directly leads to the increase of water resources utilization and aggravates the degree of water resources shortage;(3)The pattern of land-use types showed a non-equilibrium trend.In the region with increasing landscape heterogeneity,the overall landscape pattern was increasingly controlled by the majority of patches.The intensive land management model and drip irrigation under mulch have improved the utilization efficiency of water resources and saved water resources from engineering renovation and irrigation management. 展开更多
关键词 arid area manas River Basin land use/cover change landscape pattern remote sensing driving force
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DirectShow在多媒体开发中的应用 被引量:8
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作者 白燕 谢磊 曾光裕 《计算机系统应用》 北大核心 2004年第8期67-69,共3页
DirectShow是微软提供的流媒体处理的开发框架,该框架定义了如何在已有的或新建的Filter之间传送流媒体数据。本文系统地介绍了利用DirectShow进行多媒体应用开发的基本原理以及基本方法,并通过具体的实例详细阐述了如何使用Filter Grap... DirectShow是微软提供的流媒体处理的开发框架,该框架定义了如何在已有的或新建的Filter之间传送流媒体数据。本文系统地介绍了利用DirectShow进行多媒体应用开发的基本原理以及基本方法,并通过具体的实例详细阐述了如何使用Filter Graph Manager提供的智能连接机制以及选取Filter的数据传送模式进行多媒体应用的开发。 展开更多
关键词 DIRECTSHOW 多媒体开发 开发框架 FILTER GRAPH manaser 智能连接 数据传送模式
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基于移动GIS的移动物流信息管理系统设计 被引量:14
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作者 葛小三 郑鹏辉 薛华柱 《河南理工大学学报(自然科学版)》 CAS 北大核心 2017年第5期41-46,共6页
针对物流企业在物流信息管理中存在的信息交互性低、信息获取延时问题,利用Android移动设备、移动GIS和Web Service技术结合快递业务中快递人员对物流路径规划的需求,设计开发了移动物流信息管理系统。本系统主要利用ArcGIS Server技术... 针对物流企业在物流信息管理中存在的信息交互性低、信息获取延时问题,利用Android移动设备、移动GIS和Web Service技术结合快递业务中快递人员对物流路径规划的需求,设计开发了移动物流信息管理系统。本系统主要利用ArcGIS Server技术和改进的克隆选择算法将最短路径规划图以地图服务的形式推送给快递人员的安卓智能设备并在以ArcGIS Runtime for Android为框架开发的移动客户端上显示。本物流信息管理系统实现了物流信息存储管理、最佳投递路线规划、快递员位置监控等功能,改进了现有物流信息管理平台的服务模式。 展开更多
关键词 移动GIS 物流信息管理系统 路径优化
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大肠杆菌6-磷酸甘露糖异构酶基因的克隆与表达 被引量:3
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作者 张倩 廖玉才 +2 位作者 陈方方 董璇 李和平 《华中农业大学学报》 CAS CSCD 北大核心 2006年第5期461-464,共4页
通过PCR克隆了大肠杆菌6-磷酸甘露糖异构酶基因manA,分别构建了含manA基因的原核和真核生物表达载体。在大肠杆菌中经IPTG诱导表达的GST-PMI融合蛋白,经亲和层析纯化和PreScission蛋白酶酶切,SDS-PAGE分析证实PMI蛋白为42 ku。对转manA... 通过PCR克隆了大肠杆菌6-磷酸甘露糖异构酶基因manA,分别构建了含manA基因的原核和真核生物表达载体。在大肠杆菌中经IPTG诱导表达的GST-PMI融合蛋白,经亲和层析纯化和PreScission蛋白酶酶切,SDS-PAGE分析证实PMI蛋白为42 ku。对转manA基因的拟南芥进行PCR分析表明,manA基因已稳定整合到拟南芥基因组中。氯酚红显色反应证实,整合到拟南芥基因组中的manA基因能表达具有6-磷酸甘露糖异构酶活性的PMI蛋白。这些结果说明,克隆的manA基因可在细菌和高等植物中高效表达。 展开更多
关键词 manA基因 6-磷酸甘露糖异构酶活性 细菌诱导表达 拟南芥转化
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转β-甘露聚糖酶基因manA小鼠的制备 被引量:2
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作者 李紫聪 张茂 +2 位作者 许惠 刘德武 吴珍芳 《广东农业科学》 CAS CSCD 北大核心 2013年第14期144-146,150,共4页
为了获得β-甘露聚糖酶基因manA的转基因小鼠,从黑曲霉中克隆得到β-甘露聚糖酶基因manA,进行体外表达检测甘露聚糖酶活性后,将此基因插入到含有猪腮腺分泌蛋白(PSP)基因启动子的表达载体pPSPBGPneo中,得到在腮腺组织特异表达β-甘露聚... 为了获得β-甘露聚糖酶基因manA的转基因小鼠,从黑曲霉中克隆得到β-甘露聚糖酶基因manA,进行体外表达检测甘露聚糖酶活性后,将此基因插入到含有猪腮腺分泌蛋白(PSP)基因启动子的表达载体pPSPBGPneo中,得到在腮腺组织特异表达β-甘露聚糖酶基因manA的载体pPSP-manA,总长为16.3 kb,将其进行线性化后回收得到高质量DNA片段,通过显微注射得到17只原代小鼠,进行PCR和Southern blot检测发现有6只阳性转基因小鼠,表明转β-甘露聚糖酶基因manA小鼠制备成功。 展开更多
关键词 Β-甘露聚糖酶 manA基因 转基因小鼠
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熔融法皂化EVA接枝MANa离聚物的制备及影响因素的研究 被引量:2
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作者 夏英 邓亚男 +2 位作者 段效威 孙强 王爽 《弹性体》 CAS 2013年第5期7-11,共5页
采用熔融法进行乙烯-醋酸乙烯酯共聚物(EVA)与马来酸钠(MANa)的接枝反应,并利用红外光谱对接枝物(EVAL-g-MANa)的结构进行了表征。结果表明,接枝物在1 575cm-1处出现了宽的羧酸盐的C=O特征伸缩振动峰,证明MANa已与EVAL发生接枝反应,生... 采用熔融法进行乙烯-醋酸乙烯酯共聚物(EVA)与马来酸钠(MANa)的接枝反应,并利用红外光谱对接枝物(EVAL-g-MANa)的结构进行了表征。结果表明,接枝物在1 575cm-1处出现了宽的羧酸盐的C=O特征伸缩振动峰,证明MANa已与EVAL发生接枝反应,生成了离聚物。运用正交设计分析了单体浓度、反应时间、反应温度、转速等因素对EVAL-g-MANa离聚物接枝率的影响,优化了离聚物的制备工艺。 展开更多
关键词 EVAL MANa 离聚物 正交实验 影响因素
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左旋咪唑对MANA诱发大鼠食管癌前病变预防作用的研究 被引量:1
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作者 曹中亮 张玉林 +2 位作者 齐伟 杨廷桐 朱明 《新乡医学院学报》 CAS 1991年第4期255-257,共3页
本文旨在研究左旋咪唑(Levamisole)对食管癌的预防作用。食管癌前病变是食管癌发病过程中的必经阶段,防治食管癌前病变是预防食管癌的重要措施。Wistar大鼠82只,用甲基戊基亚硝胺(N—methyi—N—amylnitrosamine,简称MANA)诱发食管上皮... 本文旨在研究左旋咪唑(Levamisole)对食管癌的预防作用。食管癌前病变是食管癌发病过程中的必经阶段,防治食管癌前病变是预防食管癌的重要措施。Wistar大鼠82只,用甲基戊基亚硝胺(N—methyi—N—amylnitrosamine,简称MANA)诱发食管上皮增生,经105天实验显示左旋咪唑(每天10mg/kg,6次/周)具有与太洛龙相似的抑制食管癌前病变作用,并降低血清中MANA的含量。但是,左旋咪唑使大鼠体重增加变快,而太洛龙则使之变慢。结果表明左旋咪唑为优。 展开更多
关键词 左旋咪唑 食管肿瘤 癌前病变 MANA
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毛尖茶对甲基戊基亚硝胺诱发大鼠食管癌作用的影响 被引量:1
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作者 杨廷桐 许春雷 +1 位作者 刘红军 刘明林 《新乡医学院学报》 CAS 1990年第2期80-84,共5页
本文以大鼠为实验对象,探讨河南信阳毛尖茶对 MANA 所诱发大鼠食管上皮增生、癌变的抑制作用。本实验发现:致癌物组不典型增生为45%。茶加致癌物组为22.5%(P<0.05),两组间有显著差异。在癌的发生数量上,致癌物组为50%,茶加致癌物组为... 本文以大鼠为实验对象,探讨河南信阳毛尖茶对 MANA 所诱发大鼠食管上皮增生、癌变的抑制作用。本实验发现:致癌物组不典型增生为45%。茶加致癌物组为22.5%(P<0.05),两组间有显著差异。在癌的发生数量上,致癌物组为50%,茶加致癌物组为20%(P<0.01),两组间有极显著差异。结论为:毛尖茶对 MANA 诱发大鼠食管上皮增生癌变有明显的抑制作用。 展开更多
关键词 食管癌 毛尖茶 MANA 癌变
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