The Manchurian walnut(Juglans mandshurica Maxim.) is rich in proteins, whereas this resource has not been used efficiently. The antifatigue, antioxidative and immunoregulatory effects of Manchurian walnut hydrolysate ...The Manchurian walnut(Juglans mandshurica Maxim.) is rich in proteins, whereas this resource has not been used efficiently. The antifatigue, antioxidative and immunoregulatory effects of Manchurian walnut hydrolysate peptides(MWHPs)were evaluated in this study. MWHPs with a degree of hydrolysis of 32.23% were ultrafiltered and divided into three fractions,namely, high(> 10 k Da), medium(3–10 kDa), and low molecular weight(< 3 kDa), and then fed to mice continuously at doses of 200, 400 or 800 mg/(kg·d). The antifatigue, antioxidative, and immunoregulatory effects of the peptides were tested on the second and fourth weeks of MWHP administration. Results showed that low-molecular-weight MWHPs exerted significant antifatigue(prolonging swimming time, elevating liver glycogen contents, and reducing lactic acid contents), antioxidative(enhancing superoxide dismutase(SOD), GSH-Px, and catalase(CAT) activities and reducing malondialdehyde(MDA) content), and immunoregulatory(raising the immune-organ index and promoting T-lymphocyte proliferation and s Ig A secretion in the intestinal tract) effects. This research indicates that MWHPs have potential applications in health care and may be developed as a base for new functional foods.展开更多
目的：探讨核桃楸皮水提物、呋塞米以及二者合用对大鼠肺水肿的保护作用。方法大鼠40只，随机分为正常组，肺水肿组，核桃楸皮水提物给药组，呋塞米给药组，核桃楸皮水提物、呋塞米联合给药组，每组8只，除正常组外，其余各组采用一次...目的：探讨核桃楸皮水提物、呋塞米以及二者合用对大鼠肺水肿的保护作用。方法大鼠40只，随机分为正常组，肺水肿组，核桃楸皮水提物给药组，呋塞米给药组，核桃楸皮水提物、呋塞米联合给药组，每组8只，除正常组外，其余各组采用一次性大鼠尾静脉注射油酸0．27 ml／kg 制作急性肺水肿模型。核桃楸皮水提物给药组：于油酸注射前用核桃楸皮水提物灌胃给药，每24 h 给药1次，共给药3次；呋塞米给药组：于油酸注射后8 min尾静脉注射呋塞米1 ml／kg；核桃楸皮水提物、呋塞米联合给药组：2种药物共同给药，给药方法同上。记录各组大鼠肺重系数，并检测血清白细胞介素－1β（IL －1β）含量。结果核桃楸皮水提物和呋塞米及联合用药均具有降低肺水肿大鼠的肺重系数和血清中 IL －1β的作用，联合用药后作用明显优于核桃楸皮水提物或呋塞米。结论核桃楸皮水提物和呋塞米对肺水肿均有一定保护作用，且二者合用效果更加显著。展开更多
基金Supported by National High Technology Research and Development Program(No.2013AA102206-2)
文摘The Manchurian walnut(Juglans mandshurica Maxim.) is rich in proteins, whereas this resource has not been used efficiently. The antifatigue, antioxidative and immunoregulatory effects of Manchurian walnut hydrolysate peptides(MWHPs)were evaluated in this study. MWHPs with a degree of hydrolysis of 32.23% were ultrafiltered and divided into three fractions,namely, high(> 10 k Da), medium(3–10 kDa), and low molecular weight(< 3 kDa), and then fed to mice continuously at doses of 200, 400 or 800 mg/(kg·d). The antifatigue, antioxidative, and immunoregulatory effects of the peptides were tested on the second and fourth weeks of MWHP administration. Results showed that low-molecular-weight MWHPs exerted significant antifatigue(prolonging swimming time, elevating liver glycogen contents, and reducing lactic acid contents), antioxidative(enhancing superoxide dismutase(SOD), GSH-Px, and catalase(CAT) activities and reducing malondialdehyde(MDA) content), and immunoregulatory(raising the immune-organ index and promoting T-lymphocyte proliferation and s Ig A secretion in the intestinal tract) effects. This research indicates that MWHPs have potential applications in health care and may be developed as a base for new functional foods.
文摘目的：探讨核桃楸皮水提物、呋塞米以及二者合用对大鼠肺水肿的保护作用。方法大鼠40只，随机分为正常组，肺水肿组，核桃楸皮水提物给药组，呋塞米给药组，核桃楸皮水提物、呋塞米联合给药组，每组8只，除正常组外，其余各组采用一次性大鼠尾静脉注射油酸0．27 ml／kg 制作急性肺水肿模型。核桃楸皮水提物给药组：于油酸注射前用核桃楸皮水提物灌胃给药，每24 h 给药1次，共给药3次；呋塞米给药组：于油酸注射后8 min尾静脉注射呋塞米1 ml／kg；核桃楸皮水提物、呋塞米联合给药组：2种药物共同给药，给药方法同上。记录各组大鼠肺重系数，并检测血清白细胞介素－1β（IL －1β）含量。结果核桃楸皮水提物和呋塞米及联合用药均具有降低肺水肿大鼠的肺重系数和血清中 IL －1β的作用，联合用药后作用明显优于核桃楸皮水提物或呋塞米。结论核桃楸皮水提物和呋塞米对肺水肿均有一定保护作用，且二者合用效果更加显著。
