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CHIP相关基因与MPN患者心脑血管事件的风险分析
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作者 韩雪 白贝贝 +2 位作者 冯翠翠 赵森 陈烨 《中国实验血液学杂志》 CSCD 北大核心 2024年第1期190-196,共7页
目的:分析骨髓增殖性肿瘤(MPN)患者不确定潜能的克隆性造血(CHIP)相关基因突变谱和临床特征,探讨CHIP相关基因与其心脑血管事件(CCE)的相关性及可能作用机制。方法:回顾性分析2019年8月-2022年7月首都医科大学附属北京安贞医院血液科收... 目的:分析骨髓增殖性肿瘤(MPN)患者不确定潜能的克隆性造血(CHIP)相关基因突变谱和临床特征,探讨CHIP相关基因与其心脑血管事件(CCE)的相关性及可能作用机制。方法:回顾性分析2019年8月-2022年7月首都医科大学附属北京安贞医院血液科收治的73例MPN患者的临床资料和二代测序结果,采用Logistic回归分析CHIP相关基因、炎症细胞因子对MPN患者CCE的影响。结果:55例(75.3%)MPN患者检出CHIP相关基因,原发性血小板增多症(ET)和真性红细胞增多症(PV)患者CHIP相关基因各突变频率差异无统计学意义。CHIP相关基因突变以单基因形式为主,检出率从高至低依次为JAK2V617F(63.0%,46/73)、ASXL1(16.4%,12/73)、TET2(11.0%,8/73)、DNMT3A(9.6%,7/73)、SRSF2(6.9%,5/73)、SF3B1(4.1%,3/73)、TP53(1.4%,1/73)和PPMID(1.4%,1/73)。年龄>60岁患者CHIP相关基因检出率明显高于≤60岁者[91.7%(33/36)vs 59.5%(22/37)]。27例(37.0%)MPN患者伴CCE(MPN/CCE),2次CCE者5例,均为动脉事件。CCE组患者年龄(62.8±12.8 vs 53.9±15.8岁,P=0.015)、IL-1β水平(17.7±26.0vs 4.3±8.6,P=0.012)、IL-8水平(360.7±598.6 vs 108.3±317.0,P=0.045)、血栓形成史(29.6%vs 2.2%,P=0.020)和CHIP相关基因检出率(88.9%vs 67.4%,P=0.040)高于无CCE组。多因素Logistic回归分析结果显示,年龄(OR=0.917,95%CI:0.843-0.999,P=0.047)、血栓形成史(OR=34.148,95%CI:2.392-487.535,P=0.009)、任何1个CHIP相关基因突变(OR=16.065,95%CI:1.217-212.024,P=0.035)和IL-1β水平升高(OR=0.929,95%CI:0.870-0.992,P=0.027)均是MPN/CCE的独立危险因素;CHIP相关单基因突变与MPN/CCE无关,但DNMT3A(OR=88.717,95%CI:2.690-292.482,P=0.012)、ASXL1(OR=7.941,95%CI:1.045-60.353,P=0.045)突变是PV/CCE的独立危险因素。结论:MPN患者CHIP相关基因突变率高,尤其是60岁以上患者;高龄、血栓形成史、CHIP相关基因突变和IL-1β水平升高是MPN发生CCE的独立危险因素。DNMT3A、ASXL1单基因突变是PV患者CCE的独立危险因素。CHIP相关基因突变及炎症细胞因子IL-1β升高是MPN新的CCE危险因素。 展开更多
关键词 骨髓增殖性肿瘤 chip相关基因 心脑血管事件 炎症细胞因子
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替普瑞酮通过E3泛素连接酶CHIP减轻LPS引起的心肌炎症反应和心功能障碍
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作者 徐丽婷 刘颖文 +8 位作者 李健玲 林婉 王淼 余蕾 张雪 李航 王华东 吕秀秀 王一阳 《中国病理生理杂志》 CAS CSCD 北大核心 2024年第5期862-871,共10页
目的:探究替普瑞酮又称香叶基香叶基丙酮,GGA)对脂多糖(LPS)诱导的心功能障碍的治疗作用及机制。方法:(1)取8周龄C57BL/6雄性野生型小鼠和热休克蛋白70(HSP70)羧基末端相互作用蛋白(CHIP)基因敲除小鼠,随机分为对照组、LPS组、LPS+GGA组... 目的:探究替普瑞酮又称香叶基香叶基丙酮,GGA)对脂多糖(LPS)诱导的心功能障碍的治疗作用及机制。方法:(1)取8周龄C57BL/6雄性野生型小鼠和热休克蛋白70(HSP70)羧基末端相互作用蛋白(CHIP)基因敲除小鼠,随机分为对照组、LPS组、LPS+GGA组和GGA组,每组8只。用腹腔注射LPS(25 mg/kg)的方法建立模型,于LPS刺激后1 h给予小鼠腹腔注射GGA(100 mg/kg)。利用小动物超声系统评估小鼠心脏功能;采集各组小鼠血清,检测血清肌酸激酶同工酶(CK-MB)和乳酸脱氢酶(LDH)水平;HE染色观察病理学改变;ELISA检测心脏组织中炎症因子肿瘤坏死因子α(TNF-α)和白细胞介素6(IL-6)的水平;Western blot检测各组心脏组织HSP70、CHIP、核转运蛋白α2(KPNA2)、髓过氧化物酶(MPO)、血管细胞黏附分子(VCAM)和细胞间黏附分子(ICAM)的蛋白表达以及细胞核NF-κB的水平。(2)利用小鼠心肌细胞HL-1,建立LPS刺激的离体细胞炎症模型。ELISA检测细胞上清中TNF-α和IL-6的水平;Western blot检测心肌细胞中HSP70、CHIP和KPNA2蛋白表达;免疫荧光染色观察细胞核NF-κB的表达。结果:(1)GGA有效改善LPS刺激小鼠的心脏功能,显著提高射血分数和左室短轴缩短率(P<0.01),减少血清CK-MB和LDH含量(P<0.01),减轻心肌损伤。(2)GGA显著减少LPS引起的TNF-α和IL-6炎症因子的释放(P<0.01),以及NF-κB的入核,降低心肌组织中KPNA2、MPO、VCAM和ICAM蛋白表达,增加心肌组织和细胞HSP70的水平(P<0.01)。(3)在CHIP基因敲除的心肌细胞和小鼠中,GGA不能抑制LPS引起的炎症反应,失去了改善LPS刺激小鼠心脏功能的作用。结论:GGA能够减轻LPS引起的心功能障碍,其作用机制与升高HSP70的表达,促进CHIP的活化,减少NF-κB的入核,抑制炎症因子的释放有关。CHIP的敲除使GGA丧失了减轻LPS诱导的炎症反应和心肌损伤的作用。 展开更多
关键词 替普瑞酮 脂多糖 心功能障碍 chip蛋白 炎症
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A Scalable Interconnection Scheme in Many-Core Systems
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作者 Allam Abumwais Mujahed Eleyat 《Computers, Materials & Continua》 SCIE EI 2023年第10期615-632,共18页
Recent architectures of multi-core systems may have a relatively large number of cores that typically ranges from tens to hundreds;therefore called many-core systems.Such systems require an efficient interconnection n... Recent architectures of multi-core systems may have a relatively large number of cores that typically ranges from tens to hundreds;therefore called many-core systems.Such systems require an efficient interconnection network that tries to address two major problems.First,the overhead of power and area cost and its effect on scalability.Second,high access latency is caused by multiple cores’simultaneous accesses of the same shared module.This paper presents an interconnection scheme called N-conjugate Shuffle Clusters(NCSC)based on multi-core multicluster architecture to reduce the overhead of the just mentioned problems.NCSC eliminated the need for router devices and their complexity and hence reduced the power and area costs.It also resigned and distributed the shared caches across the interconnection network to increase the ability for simultaneous access and hence reduce the access latency.For intra-cluster communication,Multi-port Content Addressable Memory(MPCAM)is used.The experimental results using four clusters and four cores each indicated that the average access latency for a write process is 1.14785±0.04532 ns which is nearly equal to the latency of a write operation in MPCAM.Moreover,it was demonstrated that the average read latency within a cluster is 1.26226±0.090591 ns and around 1.92738±0.139588 ns for read access between cores from different clusters. 展开更多
关键词 many-core MULTI-CORE N-conjugate shuffle multi-port content addressable memory interconnection network
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Typhoon Case Comparison Analysis Between Heterogeneous Many-Core and Homogenous Multicore Supercomputing Platforms
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作者 LIU Xin YU Xiaolin +5 位作者 ZHAO Haoran HAN Qiqi ZHANG Jie WANG Chengzhi MA Weiwei XU Da 《Journal of Ocean University of China》 SCIE CAS CSCD 2023年第2期324-334,共11页
In this paper,a typical experiment is carried out based on a high-resolution air-sea coupled model,namely,the coupled ocean-atmosphere-wave-sediment transport(COAWST)model,on both heterogeneous many-core(SW)and homoge... In this paper,a typical experiment is carried out based on a high-resolution air-sea coupled model,namely,the coupled ocean-atmosphere-wave-sediment transport(COAWST)model,on both heterogeneous many-core(SW)and homogenous multicore(Intel)supercomputing platforms.We construct a hindcast of Typhoon Lekima on both the SW and Intel platforms,compare the simulation results between these two platforms and compare the key elements of the atmospheric and ocean modules to reanalysis data.The comparative experiment in this typhoon case indicates that the domestic many-core computing platform and general cluster yield almost no differences in the simulated typhoon path and intensity,and the differences in surface pressure(PSFC)in the WRF model and sea surface temperature(SST)in the short-range forecast are very small,whereas a major difference can be identified at high latitudes after the first 10 days.Further heat budget analysis verifies that the differences in SST after 10 days are mainly caused by shortwave radiation variations,as influenced by subsequently generated typhoons in the system.These typhoons generated in the hindcast after the first 10 days attain obviously different trajectories between the two platforms. 展开更多
关键词 heterogeneous many-core supercomputing platform homogenous multicore supercomputing platform comparison analysis typhoon case
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Simulation and fabrication of in vitro microfluidic microelectrode array chip for patterned culture and electrophysiological detection of neurons
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作者 Yan Yang Shihong Xu +7 位作者 Yu Deng Yaoyao Liu Kui Zhang Shiya Lv Longze Sha Qi Xu Xinxia Cai Jinping Luo 《Nanotechnology and Precision Engineering》 EI CAS CSCD 2024年第2期1-10,共10页
To enable the detection and modulation of modularized neural networks in vitro,this study proposes a microfluidic microelectrode array chip for the cultivation,compartmentalization,and control of neural cells.The chip... To enable the detection and modulation of modularized neural networks in vitro,this study proposes a microfluidic microelectrode array chip for the cultivation,compartmentalization,and control of neural cells.The chip was designed based on the specific structure of neurons and the requirements for detection and modulation.Finite-element analysis of the chip’s flow field was conducted using the COMSOL Multiphysics software,and the simulation results show that the liquid within the chip can flow smoothly,ensuring stable flow fields that facilitate the uniform growth of neurons within the microfluidic channels.By employing MEMS technology in combination with nanomaterial modification techniques,the microfluidic microelectrode array chip was fabricated successfully.Primary hippocampal neurons were cultured on the chip,forming a well-defined neural network.Spontaneous electrical activity of the detected neurons was recorded,exhibiting a 23.7%increase in amplitude compared to neuronal discharges detected on an open-field microelectrode array.This study provides a platform for the precise detection and modulation of patterned neuronal growth in vitro,potentially serving as a novel tool in neuroscience research. 展开更多
关键词 Microfluidic Microelectrode array In vitro Neural chip
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On-chip quantum NOON state sensing for temperature and humidity
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作者 Weihong Luo Chao Wu +5 位作者 Yuxing Du Chang Zhao Miaomiao Yu Pingyu Zhu Kaikai Zhang Ping Xu 《Chinese Physics B》 SCIE EI CAS CSCD 2024年第10期15-20,共6页
A maximal photon number entangled state,namely NOON state,can be adopted for sensing with a quantum enhancedprecision.In this work,we designed silicon quantum photonic chips containing two types of Mach-Zehnder interf... A maximal photon number entangled state,namely NOON state,can be adopted for sensing with a quantum enhancedprecision.In this work,we designed silicon quantum photonic chips containing two types of Mach-Zehnder interferometerswherein the two-photon NOON state,sensing element for temperature or humidity,is generated.Compared with classicallight or single photon case,two-photon NOON state sensing shows a solid enhancement in the sensing resolution andprecision.As the first demonstration of on-chip quantum photonic sensing,it reveals the advantages of photonic chips forhigh integration density,small-size,stability for multiple-parameter sensing serviceability.A higher sensing precision isexpected to beat the standard quantum limit with a higher photon number NOON state. 展开更多
关键词 quantum sensing NOON state photonic chip
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A Fully-Integrated Memristor Chip for Edge Learning
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作者 Yanhong Zhang Liang Chu Wenjun Li 《Nano-Micro Letters》 SCIE EI CAS CSCD 2024年第9期123-127,共5页
It is still challenging to fully integrate computing in memory chip as edge learning devices.In recent work published on Science,a fully-integrated chip based on neuromorphic memristors was developed for edge learning... It is still challenging to fully integrate computing in memory chip as edge learning devices.In recent work published on Science,a fully-integrated chip based on neuromorphic memristors was developed for edge learning as artificial neural networks with functionality of synapses,dendrites,and somas.A crossbar-array memristor chip facilitated edge learning including hardware realization,learning algorithm,and cycle-parallel sign-and threshold-based learning(STELLAR)scheme.The motion control and demonstration platforms were executed to improve the edge learning ability for adapting to new scenarios. 展开更多
关键词 Computing in memory Edge learning Fully-integrated chip
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Simplified quantitative analysis method and its application in the insitu synthesized copper-based azide chips
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作者 Jie Ren Yunfeng Li +3 位作者 Mingyu Li Xingyu Wu Jiabao Wang Qingxuan Zeng 《Defence Technology(防务技术)》 SCIE EI CAS CSCD 2024年第2期309-316,共8页
Copper-based azide(Cu(N_(3))2 or CuN_(3),CA)chips synthesized by in-situ azide reaction and utilized in miniaturized explosive systems has become a hot research topic in recent years.However,the advantages of in-situ ... Copper-based azide(Cu(N_(3))2 or CuN_(3),CA)chips synthesized by in-situ azide reaction and utilized in miniaturized explosive systems has become a hot research topic in recent years.However,the advantages of in-situ synthesis method,including small size and low dosage,bring about difficulties in quantitative analysis and differences in ignition capabilities of CA chips.The aim of present work is to develop a simplified quantitative analysis method for accurate and safe analysis of components in CA chips to evaluate and investigate the corresponding ignition ability.In this work,Cu(N_(3))2 and CuN_(3)components in CA chips were separated through dissolution and distillation by utilizing the difference in solubility and corresponding content was obtained by measuring N_(3)-concentration through spectrophotometry.The spectrophotometry method was optimized by studying influencing factors and the recovery rate of different separation methods was studied,ensuring the accuracy and reproducibility of test results.The optimized method is linear in range from 1.0-25.0 mg/L,with a correlation coefficient R^(2)=0.9998,which meets the requirements of CA chips with a milligram-level content test.Compared with the existing ICP method,component analysis results of CA chips obtained by spectrophotometry are closer to real component content in samples and have satisfactory accuracy.Moreover,as its application in miniaturized explosive systems,the ignition ability of CA chips with different component contents for direct ink writing CL-20 and the corresponding mechanism was studied.This study provided a basis and idea for the design and performance evaluation of CA chips in miniaturized explosive systems. 展开更多
关键词 Copper-based azide chips SPECTROPHOTOMETRY Separation method Quantitative analysis Ignition ability
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A novel integrated microfluidic chip for on-demand electrostatic droplet charging and sorting
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作者 Jinhui Yao Chunhua He +5 位作者 Jianxin Wang Canfeng Yang Ye Jiang Zhiyong Liu Guanglan Liao Tielin Shi 《Bio-Design and Manufacturing》 SCIE EI CAS CSCD 2024年第1期31-42,共12页
On-demand droplet sorting is extensively applied for the efficient manipulation and genome-wide analysis of individual cells.However,state-of-the-art microfluidic chips for droplet sorting still suffer from low sortin... On-demand droplet sorting is extensively applied for the efficient manipulation and genome-wide analysis of individual cells.However,state-of-the-art microfluidic chips for droplet sorting still suffer from low sorting speeds,sample loss,and labor-intensive preparation procedures.Here,we demonstrate the development of a novel microfluidic chip that integrates droplet generation,on-demand electrostatic droplet charging,and high-throughput sorting.The charging electrode is a copper wire buried above the nozzle of the microchannel,and the deflecting electrode is the phosphate buffered saline in the microchannel,which greatly simplifies the structure and fabrication process of the chip.Moreover,this chip is capable of high-frequency droplet generation and sorting,with a frequency of 11.757 kHz in the drop state.The chip completes the selective charging process via electrostatic induction during droplet generation.On-demand charged microdroplets can arbitrarilymove to specific exit channels in a three-dimensional(3D)-deflected electric field,which can be controlled according to user requirements,and the flux of droplet deflection is thereby significantly enhanced.Furthermore,a lossless modification strategy is presented to improve the accuracy of droplet deflection or harvest rate from 97.49% to 99.38% by monitoring the frequency of droplet generation in real time and feeding it back to the charging signal.This chip has great potential for quantitative processing and analysis of single cells for elucidating cell-to-cell variations. 展开更多
关键词 Copper wire Droplet generation Droplet sorting Microfluidic chips On-demand charging
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Single event effects evaluation on convolution neural network in Xilinx 28 nm system on chip
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作者 赵旭 杜雪成 +4 位作者 熊旭 马超 杨卫涛 郑波 周超 《Chinese Physics B》 SCIE EI CAS CSCD 2024年第7期638-644,共7页
Convolutional neural networks(CNNs) exhibit excellent performance in the areas of image recognition and object detection, which can enhance the intelligence level of spacecraft. However, in aerospace, energetic partic... Convolutional neural networks(CNNs) exhibit excellent performance in the areas of image recognition and object detection, which can enhance the intelligence level of spacecraft. However, in aerospace, energetic particles, such as heavy ions, protons, and alpha particles, can induce single event effects(SEEs) that lead CNNs to malfunction and can significantly impact the reliability of a CNN system. In this paper, the MNIST CNN system was constructed based on a 28 nm systemon-chip(SoC), and then an alpha particle irradiation experiment and fault injection were applied to evaluate the SEE of the CNN system. Various types of soft errors in the CNN system have been detected, and the SEE cross sections have been calculated. Furthermore, the mechanisms behind some soft errors have been explained. This research will provide technical support for the design of radiation-resistant artificial intelligence chips. 展开更多
关键词 single event effects convolutional neural networks alpha particle system on chip fault injection
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Rapid fabrication of modular 3D paper-basedmicrofluidic chips using projection-based 3D printing
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作者 Mingjun Xie Zexin Fu +5 位作者 Chunfei Lu Sufan Wu Lei Pan Yong He Yi Sun Ji Wang 《Bio-Design and Manufacturing》 SCIE EI CAS CSCD 2024年第5期611-623,共13页
Paper-based microchips have different advantages,such as better biocompatibility,simple production,and easy handling,making them promising candidates for clinical diagnosis and other fields.This study describes ametho... Paper-based microchips have different advantages,such as better biocompatibility,simple production,and easy handling,making them promising candidates for clinical diagnosis and other fields.This study describes amethod developed to fabricate modular three-dimensional(3D)paper-based microfluidic chips based on projection-based 3D printing(PBP)technology.A series of two-dimensional(2D)paper-based microfluidic modules was designed and fabricated.After evaluating the effect of exposure time on the accuracy of the flow channel,the resolution of this channel was experimentally analyzed.Furthermore,several 3D paper-based microfluidic chips were assembled based on the 2D ones using different methods,with good channel connectivity.Scaffold-based 2D and hydrogel-based 3D cell culture systems based on 3D paper-based microfluidic chips were verified to be feasible.Furthermore,by combining extrusion 3D bioprinting technology and the proposed 3D paper-based microfluidic chips,multiorgan microfluidic chips were established by directly printing 3D hydrogel structures on 3D paperbased microfluidic chips,confirming that the prepared modular 3D paper-based microfluidic chip is potentially applicable in various biomedical applications. 展开更多
关键词 Paper-based microfluidic chip Projection-based 3D printing(PBP) Modularization Cell culture
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Efficient stochastic parallel gradient descent training for on-chip optical processor
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作者 Yuanjian Wan Xudong Liu +4 位作者 Guangze Wu Min Yang Guofeng Yan Yu Zhang Jian Wang 《Opto-Electronic Advances》 SCIE EI CAS CSCD 2024年第4期5-15,共11页
In recent years,space-division multiplexing(SDM)technology,which involves transmitting data information on multiple parallel channels for efficient capacity scaling,has been widely used in fiber and free-space optical... In recent years,space-division multiplexing(SDM)technology,which involves transmitting data information on multiple parallel channels for efficient capacity scaling,has been widely used in fiber and free-space optical communication sys-tems.To enable flexible data management and cope with the mixing between different channels,the integrated reconfig-urable optical processor is used for optical switching and mitigating the channel crosstalk.However,efficient online train-ing becomes intricate and challenging,particularly when dealing with a significant number of channels.Here we use the stochastic parallel gradient descent(SPGD)algorithm to configure the integrated optical processor,which has less com-putation than the traditional gradient descent(GD)algorithm.We design and fabricate a 6×6 on-chip optical processor on silicon platform to implement optical switching and descrambling assisted by the online training with the SPDG algorithm.Moreover,we apply the on-chip processor configured by the SPGD algorithm to optical communications for optical switching and efficiently mitigating the channel crosstalk in SDM systems.In comparison with the traditional GD al-gorithm,it is found that the SPGD algorithm features better performance especially when the scale of matrix is large,which means it has the potential to optimize large-scale optical matrix computation acceleration chips. 展开更多
关键词 optical communications optical signal processing channel descrambling optical neural network chip silicon photonics
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Effects of Using Softwood Pellet Biochar Prepared at Different Temperatures with Grass Chippings on Retention of Heavy Metals in Contaminated Soils
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作者 Kyari Umar Dunoma Limin Ma +14 位作者 Xingquan Shu Haiyan Yu Weiwei Zhang Chengcheng Bu Yong-Sik Wang Jiahong Luo Guangyao Chen Jinpeng Yu Ru Zhang Yuchen Han Haoyu Zeng Matthew S. Wisseh Amina Grema Mustafa Mohammad Auwal Saidu Mufidat Mamman Khan 《Open Journal of Polymer Chemistry》 2024年第3期146-166,共21页
Heavy metals have been viewed as hazardous environmental pollutants, and anthropogenic activities due to their high toxicity and persistent nature in the environment. Anthropogenic activities such as artisanal mining,... Heavy metals have been viewed as hazardous environmental pollutants, and anthropogenic activities due to their high toxicity and persistent nature in the environment. Anthropogenic activities such as artisanal mining, industrial activities, improper usage of fertilizers and pesticides, and indiscriminate open waste disposal bring about an increase in the presence of heavy metals in the environment. In the Keffi Metropolis, different elements lead to land contamination which debilitates soil quality, plant survival, human well-being, and the environment as a result of extensive dispersion or quantity of heavy metals in the soil and water. In recent years, biochar has emerged as a promising soil amendment for mitigating heavy metal pollution due to its unique physicochemical properties. This paper provides the effects of softwood pellet biochar on the retention of heavy metals in contaminated soils. A microcosm experiment was carried out to investigate the effects of biochar on the retention of heavy metals in contaminated soils. This research aimed to give an overview of the effects of softwood biochar at different temperatures (550˚C and 700˚C) on the retention of heavy metals and metalloids released from the soil during water inundation. The results show that the addition of organic matter (grass chippings) minimizes heavy metal mobilization. Also, biochar at high temperatures is more effective than those at low temperatures. The expected outcome of the research analysis includes providing insights into the role of biochar in retaining heavy metal contamination and further understanding the use of biochar as a sorbent for the management of contaminated soil. 展开更多
关键词 Softwood Biochar Heavy Metal Contaminated Soil Grass chips Keffi Metropolis MICROCOSM
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Flip Chip技术在集成电路封装中的应用
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作者 黄家友 《集成电路应用》 2024年第3期56-57,共2页
阐述从集成电路封装发展现状、Flip Chip技术内涵、Flip Chip技术在集成电路封装中的应用剖析、市场发展展望等多个角度,探讨在集成电路封装中,应用Flip Chip技术的必要性和重要性。
关键词 集成电路 Flip chip技术 电子器件封装
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An electrochemiluminescent magneto-immunosensor for ultrasensitive detection of hs-cTnI on a microfluidic chip
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作者 Yun Hui Zhen Zhao +7 位作者 Weiliang Shu Fengshan Shen Weijun Kong Shengyong Geng Zhen Xu Tianzhun Wu Wenhua Zhou Xuefeng Yu 《Nanotechnology and Precision Engineering》 EI CAS CSCD 2024年第3期13-23,共11页
Sensitive detection and precise quantitation of trace-level crucial biomarkers in a complex sample matrix has become an important area of research.For example,the detection of high-sensitivity cardiac troponin I (hs-c... Sensitive detection and precise quantitation of trace-level crucial biomarkers in a complex sample matrix has become an important area of research.For example,the detection of high-sensitivity cardiac troponin I (hs-cTnI) is strongly recommended in clinical guidelines for early diagnosis of acute myocardial infarction.Based on the use of an electrode modified by single-walled carbon nanotubes (SWCNTs) and a Ru(bpy)32+-doped silica nanoparticle (Ru@SiO2)/tripropylamine (TPA) system,a novel type of electrochemiluminescent (ECL) magnetoimmunosensor is developed for ultrasensitive detection of hs-cTnI.In this approach,a large amount of[Ru(bpy)3]2+is loaded in SiO2(silica nanoparticles) as luminophores with high luminescent efficiency and SWCNTs as electrode surface modification material with excellent electrooxidation ability for TPA.Subsequently,a hierarchical micropillar array of microstructures is fabricated with a magnet placed at each end to efficiently confine a single layer of immunomagnetic microbeads on the surface of the electrode and enable 7.5-fold signal enhancement In particular,the use of transparent SWCNTs to modify a transparent ITO electrode provides a two-order-of-magnitude ECL signal amplification.A good linear calibration curve is developed for hs-cTnI concentrations over a wide range from 10 fg/ml to 10 ng/ml,with the limit of detection calculated as 8.720 fg/ml (S/N=3).This ultrasensitive immunosensor exhibits superior detection performance with remarkable stability,reproducibility,and selectivity.Satisfactory recoveries are obtained in the detection of hs-cTnI in human serum,providing a potentia analysis protocol for clinical applications. 展开更多
关键词 Electrochemiluminescent magneto-immunosensor Microfluidic chip High-sensitivity cardiac troponin I Single-walled carbon nanotube [Ru(bpy)3]2+-doped silica nanoparticle
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Chiplet技术推动半导体产业可持续发展的研究
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作者 张志伟 冯明宪 +1 位作者 田果 王世权 《无线互联科技》 2024年第2期19-22,共4页
随着半导体产业面临莫尔定律的瓶颈,寻找新的技术推动力变得至关重要。文章将焦点放在Chiplet技术,这是一种可突破物理和经济限制、进一步提升性能的新兴技术。Chiplet技术利用模组化策略,不仅能提供更高的效能,且符合可持续发展的要求... 随着半导体产业面临莫尔定律的瓶颈,寻找新的技术推动力变得至关重要。文章将焦点放在Chiplet技术,这是一种可突破物理和经济限制、进一步提升性能的新兴技术。Chiplet技术利用模组化策略,不仅能提供更高的效能,且符合可持续发展的要求。文章深入探讨了Chiplet技术如何在莫尔定律遇到挑战的情况下推动半导体产业的可持续发展,并期望提供新的思维和发展方向。 展开更多
关键词 半导体产业 芯片发展 chiplet技术
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副溶血弧菌QsvR ChIP-qPCR方法的建立 被引量:2
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作者 张苗苗 李雪 +2 位作者 陆仁飞 张义全 周敏 《江苏大学学报(医学版)》 CAS 2023年第6期516-520,527,共6页
目的:建立副溶血弧菌QsvR的染色质免疫共沉淀实时定量PCR(ChIP-qPCR)实验方法。方法:通过热激转化和转化结合将标记2×Flag标签的qsvR片段转入qsvR突变株(ΔqsvR)中,阿拉伯糖诱导表达QsvR-2×Flag蛋白,通过甲醛与基因组DNA进行... 目的:建立副溶血弧菌QsvR的染色质免疫共沉淀实时定量PCR(ChIP-qPCR)实验方法。方法:通过热激转化和转化结合将标记2×Flag标签的qsvR片段转入qsvR突变株(ΔqsvR)中,阿拉伯糖诱导表达QsvR-2×Flag蛋白,通过甲醛与基因组DNA进行交联形成2×Flag-QsvR-DNA复合物,将基因组DNA超声裂解为100~1000 bp大小不等的片段,通过特异性抗原抗体反应将蛋白质-DNA复合物沉淀下来,高盐和加热解交联,回收DNA进行qPCR定量DNA,分析副溶血弧菌体内QsvR与靶基因的结合情况。结果:成功构建出实验菌株ΔqsvR/pBAD33-qsvR-2×Flag,以不加阿拉伯糖诱导为对照,经阿拉伯糖诱导菌株的aphA、opaR、exsB和vtrA的免疫共沉淀量明显较高,表明在副溶血弧菌体内QsvR与aphA、opaR、exsB和vtrA具有结合作用。结论:成功建立了副溶血弧菌QsvR的ChIP-qPCR实验方法,可用于原核生物体内蛋白质-DNA相互作用的研究。 展开更多
关键词 chip-qPCR 蛋白质-DNA复合物 副溶血弧菌 QsvR
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CHIP蛋白在疾病中的作用研究进展
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作者 王韶畅 代紫璠 +1 位作者 赵林芃 商瑜 《北京师范大学学报(自然科学版)》 CAS CSCD 北大核心 2023年第4期528-536,共9页
CHIP(carboxyl terminus of Hsc70-interacting protein)又称STUB1(STIP1 homology and U-box containing protein 1),是一种分子伴侣结合蛋白,具有E3泛素连接酶活性.研究表明,CHIP蛋白在细胞体内通过蛋白质量控制系统调控细胞代谢、分... CHIP(carboxyl terminus of Hsc70-interacting protein)又称STUB1(STIP1 homology and U-box containing protein 1),是一种分子伴侣结合蛋白,具有E3泛素连接酶活性.研究表明,CHIP蛋白在细胞体内通过蛋白质量控制系统调控细胞代谢、分化、增殖、迁移和肿瘤发生发展等多种生理病理过程.本文对CHIP在癌症及多种常见疾病中的作用进行综述,以期为临床治疗和药物开发提供潜在突破点. 展开更多
关键词 chip 降解 泛素化 癌症 疾病
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泛素E3连接酶CHIP介导RCC2的泛素化及降解
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作者 李贝 范宇宸 赵博 《石河子大学学报(自然科学版)》 CAS 北大核心 2023年第3期327-334,共8页
目的 本课题组前期构建了一种正交泛素传递方法,并发现染色体浓缩调节蛋白2(RCC2)是泛素连接酶CHIP的潜在底物。本实验旨在验证CHIP是否能介导RCC2的泛素化及降解,以期通过CHIP来泛素化调控RCC2的表达和功能。方法 首先构建CHIP及其突... 目的 本课题组前期构建了一种正交泛素传递方法,并发现染色体浓缩调节蛋白2(RCC2)是泛素连接酶CHIP的潜在底物。本实验旨在验证CHIP是否能介导RCC2的泛素化及降解,以期通过CHIP来泛素化调控RCC2的表达和功能。方法 首先构建CHIP及其突变体质粒,验证CHIP与RCC2是否有相互作用;然后构建pLVX-RCC2-FLAG重组质粒并转染进CHIP敲减细胞株(shCHIP)、HEK293细胞株、CHIP过表达细胞株(OE CHIP),通过免疫共沉淀下拉富集RCC2-FLAG并检测其泛素化水平;接下来在HEK293细胞中转染不同量的CHIP,以及设置CHX chase蛋白稳定性实验,检测RCC2的蛋白水平变化;最后利用泛素突变体K48R-Ub及K11R-Ub进行CHIP介导RCC2上形成泛素链的实验检测。结果 在HEK293细胞中,CHIP能够介导RCC2的泛素化,并在RCC2上形成K48和K11多聚泛素链,促进其通过蛋白酶体发生降解。结论 泛素连接酶CHIP能够介导RCC2的泛素化及降解,为实现通过CHIP来泛素化调控RCC2提供了理论基础。 展开更多
关键词 泛素化 泛素连接酶chip 染色体浓缩调节蛋白2 降解
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Flip Chip结构的IC信号完整性仿真分析
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作者 李少聪 杨录 +1 位作者 吕俊文 闫慧欣 《单片机与嵌入式系统应用》 2023年第10期12-15,共4页
针对Flip Chip封装型芯片设计过程中存在的传输线阻抗不连续与串扰过大等问题,从层叠设置与板材介质厚度两个角度提出了一种基于阻抗、串扰的仿真分析设计方法,主要涉及两个方面:对于由信号参考平面被分割而造成的阻抗突变问题,通过添... 针对Flip Chip封装型芯片设计过程中存在的传输线阻抗不连续与串扰过大等问题,从层叠设置与板材介质厚度两个角度提出了一种基于阻抗、串扰的仿真分析设计方法,主要涉及两个方面:对于由信号参考平面被分割而造成的阻抗突变问题,通过添加参考平面而使信号具有完整的回流路径,使得传输线的阻抗在平面分割处由167.5Ω降至52.5Ω;对于因布线密度过大而造成的走线之间的串扰系数偏高的问题,通过减小板材的介质厚度使传输线间串扰系数的最大值从17.26%降至14.01%。仿真结果表明,此设计方法有效降低了芯片设计中潜在的信号完整性风险,提高了芯片的可靠性和稳定性。 展开更多
关键词 Flip chip IC 信号完整性 阻抗 串扰
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