Roles of Marigold extracts (ME) on arsenic trioxide (ATO)-induced oxidative damage to pancreatic β-cells need to be further elucidated. In this study, NIT-1 cells were treated with different concentrations of and/or ...Roles of Marigold extracts (ME) on arsenic trioxide (ATO)-induced oxidative damage to pancreatic β-cells need to be further elucidated. In this study, NIT-1 cells were treated with different concentrations of and/or ATO, following by the cell viability was detected by CCK8 assay. Then, intracellular reactive oxygen species (ROS) levels, lipid peroxide (MDA) contents and superoxide dismutase (SOD) activity were measured with a fluorescence probe method and colorimetric assay, respectively. The apoptosis rate and morphology was detected and observed with hoechst 33,258 staining assay. The mRNA levels and protein expressions of nuclear factor E2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) were measured by real-time fluorescence quantitative polymerase chain reaction and protein immunoblotting assay, respectively. Our results indicated that Co-treatment with ME and ATO exacerbated the cell viability decreasing reduced by ATO, while the addition of ME after ATO treatment effectively promote the recovery of ATO reduced survival rates. The ATO group increased apoptosis (P P β-cells by modulating the activation of the Nrf2 signaling pathway.展开更多
[ Objective ] The study aimed to explore a new way for the control of Tomato Fusarium Wilt. [ Method ] Different solvents were used to prepare the ex-tracts of marigold, and the inhibitory effects of different extract...[ Objective ] The study aimed to explore a new way for the control of Tomato Fusarium Wilt. [ Method ] Different solvents were used to prepare the ex-tracts of marigold, and the inhibitory effects of different extraction solvents and different extraction parts of marigold against Tomato Fusar/um Wilt were compared. [ Result ] Among different solvent extracts of marigold, chloroform extracts had the strongest inhibitory effects against the growth of the pathogen; among the chloro- form extracts from different parts of marigold, root extract had the most obvious inhibitory effect against the disease, followed by flower and leaf extracts, and the in- hibitory effect of stem extract was the weakest. [ Conclusion ] The active components of marigold have inhibitory effect against Tomato Fusarium Wilt, and the plant has good development prospects and application value.展开更多
In laboratory experiments were evaluated TRICHOD®, FOLIGUARD®, Trichoderma sp, and watery extracts of pine, marigold and eucalyptus, respectively, against F. oxysporum. There were no significant differen...In laboratory experiments were evaluated TRICHOD®, FOLIGUARD®, Trichoderma sp, and watery extracts of pine, marigold and eucalyptus, respectively, against F. oxysporum. There were no significant differences between TRICHOD® and FOLIGUARD®. The extract of marigold of 120 g/L was the best result in reducing the area of the colony of F. oxysporum. In field pots experiment were evaluated the extract of marigold (120 g/L), potassium phosphite (400 g/L), TRICHOD and benomyl (1.5 g/L) to control F. oxysporum. Treatments were applied to the soil, 15 days after inoculation with F. oxysporum. The best obtained heights were of 32 cm with extract of marigold 120 g/L, followed by potassium phosphite with 31 cm, and the control reached 26 cm of height. The treatment with an extract of marigold 120 g/L, obtained the best dry weight average of 6.5 g, statistically different from the control. The extract of marigold presented the best efficiency against the disease, because it reduced the length of the vascular symptom in 88.5%;followed by TRICHOD®, benomyl and potassium phosphite that showed reductions of 86, 85 and 84 percent, respectively.展开更多
研究超声波强化有机溶剂提取万寿菊花粉中叶黄素的工艺过程,考察了萃取率的影响因素。结果表明,正己烷和石油醚作提取溶剂效果较好。以1.000 g万寿菊花粉为原料,超声波强化正己烷的较优提取条件为:料液比1∶20(g∶mL,以下同),超声波功率...研究超声波强化有机溶剂提取万寿菊花粉中叶黄素的工艺过程,考察了萃取率的影响因素。结果表明,正己烷和石油醚作提取溶剂效果较好。以1.000 g万寿菊花粉为原料,超声波强化正己烷的较优提取条件为:料液比1∶20(g∶mL,以下同),超声波功率300 W,超声波作用时间30 m in,叶黄素提取率93.65%;强化石油醚的较优条件为:料液比1∶20,超声波功率400 W,超声波作用时间30 m in,叶黄素提取率98.77%。展开更多
文摘Roles of Marigold extracts (ME) on arsenic trioxide (ATO)-induced oxidative damage to pancreatic β-cells need to be further elucidated. In this study, NIT-1 cells were treated with different concentrations of and/or ATO, following by the cell viability was detected by CCK8 assay. Then, intracellular reactive oxygen species (ROS) levels, lipid peroxide (MDA) contents and superoxide dismutase (SOD) activity were measured with a fluorescence probe method and colorimetric assay, respectively. The apoptosis rate and morphology was detected and observed with hoechst 33,258 staining assay. The mRNA levels and protein expressions of nuclear factor E2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) were measured by real-time fluorescence quantitative polymerase chain reaction and protein immunoblotting assay, respectively. Our results indicated that Co-treatment with ME and ATO exacerbated the cell viability decreasing reduced by ATO, while the addition of ME after ATO treatment effectively promote the recovery of ATO reduced survival rates. The ATO group increased apoptosis (P P β-cells by modulating the activation of the Nrf2 signaling pathway.
基金Supported by Fund for Highly Educated People in Yulin College(07gk013)~~
文摘[ Objective ] The study aimed to explore a new way for the control of Tomato Fusarium Wilt. [ Method ] Different solvents were used to prepare the ex-tracts of marigold, and the inhibitory effects of different extraction solvents and different extraction parts of marigold against Tomato Fusar/um Wilt were compared. [ Result ] Among different solvent extracts of marigold, chloroform extracts had the strongest inhibitory effects against the growth of the pathogen; among the chloro- form extracts from different parts of marigold, root extract had the most obvious inhibitory effect against the disease, followed by flower and leaf extracts, and the in- hibitory effect of stem extract was the weakest. [ Conclusion ] The active components of marigold have inhibitory effect against Tomato Fusarium Wilt, and the plant has good development prospects and application value.
文摘In laboratory experiments were evaluated TRICHOD®, FOLIGUARD®, Trichoderma sp, and watery extracts of pine, marigold and eucalyptus, respectively, against F. oxysporum. There were no significant differences between TRICHOD® and FOLIGUARD®. The extract of marigold of 120 g/L was the best result in reducing the area of the colony of F. oxysporum. In field pots experiment were evaluated the extract of marigold (120 g/L), potassium phosphite (400 g/L), TRICHOD and benomyl (1.5 g/L) to control F. oxysporum. Treatments were applied to the soil, 15 days after inoculation with F. oxysporum. The best obtained heights were of 32 cm with extract of marigold 120 g/L, followed by potassium phosphite with 31 cm, and the control reached 26 cm of height. The treatment with an extract of marigold 120 g/L, obtained the best dry weight average of 6.5 g, statistically different from the control. The extract of marigold presented the best efficiency against the disease, because it reduced the length of the vascular symptom in 88.5%;followed by TRICHOD®, benomyl and potassium phosphite that showed reductions of 86, 85 and 84 percent, respectively.
文摘研究超声波强化有机溶剂提取万寿菊花粉中叶黄素的工艺过程,考察了萃取率的影响因素。结果表明,正己烷和石油醚作提取溶剂效果较好。以1.000 g万寿菊花粉为原料,超声波强化正己烷的较优提取条件为:料液比1∶20(g∶mL,以下同),超声波功率300 W,超声波作用时间30 m in,叶黄素提取率93.65%;强化石油醚的较优条件为:料液比1∶20,超声波功率400 W,超声波作用时间30 m in,叶黄素提取率98.77%。
