2-haloacid dehalogenases constitute a group of dehalogenases which are capable of dehalogenating the halogenated organic compounds. So far, the 2-haloacid dehalogenases have been found in many bacteria, but not in Par...2-haloacid dehalogenases constitute a group of dehalogenases which are capable of dehalogenating the halogenated organic compounds. So far, the 2-haloacid dehalogenases have been found in many bacteria, but not in Paracoccus genus. In the present study, one enzyme 2-haloacid dehalogenase(designated as Deh99), induced by DL-2-chloropropionate(DL-2-CPA), was purified from the marine bacterium Paracoccus sp. DEH99, isolated from marine sponge Hymeniacidon perlevis. The enzyme of Deh99 was purified to homogeneity by ammonium sulfate precipitation, ion exchange chromatography(Q-Sepharose HP), and Superdex 200 gel filtration chromatography. The molecular weight of Deh99 was estimated to be 25.0 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE), and 50.0 kDa natively by gel filtration chromatography. The enzyme of Deh99 stereospecifically dehalogenated L-2-CPA to produce D-lactate, with an apparent Michaelis-Menten constant(Km) value of 0.21 mmol L-1 for L-2-CPA. The optimal pH and temperature for Deh99 activity were 10.0 and 40℃, respectively. The enzyme of Deh99 acted on short-carbon-chain 2-haloacids, with the highest activity towards monochloroacetate. The activity of Deh99 was slightly affected by DTT and EDTA, but strongly inhibited by Cu2+ and Zn2+. The enzyme of Deh99 shows unique substrate specificity and inhibitor sensitivities compared to previously characterized 2-haloacid dehalogenases and is the reported one about purified 2-haloacid dehalogenase isolated from the bacteria of Paracoccus genus.展开更多
[Objective] The aim of this study is to identify a bacterial strain isolated from ocean water from the Yellow Sea.[Method]Using 16S rRNA technique,a strain from Yellow Sea was preliminarily identified and analyzed.[Re...[Objective] The aim of this study is to identify a bacterial strain isolated from ocean water from the Yellow Sea.[Method]Using 16S rRNA technique,a strain from Yellow Sea was preliminarily identified and analyzed.[Result]One 1 521 bp fragment of 16S rRNA was amplified from the strain HZBN43;homology analysis between the yielded sequence and the 16S rRNA sequences accessed in NCBI from other strains showed that HZBN43 belonged to Bacillus,and shared 99.79% homologue with the known species of Bacillus selenatarsenatis.[Conclusion]The sequence of strain HZBN43 was obtained.However,because of the incomplete sequence,the confidence level is just 46,so other corroborations are still required for grouping HZBN43 into an exact species.展开更多
采用多种色谱分离技术,从南海深海细菌Bacillus amyloliquefaciens GAS 00152发酵液中分离得到12个化合物,经波谱数据分析分别鉴定为4-苯基-3-丁烯酰胺（1）、苯乙酰胺（2）、苯乙酸-2-（4-羟苯基）乙酯（3）、对羟基苯乙醇（4）、环（...采用多种色谱分离技术,从南海深海细菌Bacillus amyloliquefaciens GAS 00152发酵液中分离得到12个化合物,经波谱数据分析分别鉴定为4-苯基-3-丁烯酰胺（1）、苯乙酰胺（2）、苯乙酸-2-（4-羟苯基）乙酯（3）、对羟基苯乙醇（4）、环（甘氨酸-2-氨基丁酸）（5）、环（甘氨酸-亮氨酸）（6）、环（甘氨酸-L-脯氨酸）（7）、环（D-脯氨酸-L-缬氨酸）（8）、环（4-羟基脯氨酸-亮氨酸）（9）、环（N-甲基甘氨酸-苯丙氨酸）（10）、环（L-2-羟基脯氨酸-苯丙氨酸）（11）、环（2-哌啶酸-苯丙氨酸）（12）。其中,化合物1为新天然产物。测试化合物1-12对番木瓜炭疽菌和香蕉黑星菌的抑制活性,发现化合物9对两种热带水果致病菌显示出中等抗菌活性。展开更多
A bicyclic depsipeptide, chromopeptide A(1), was isolated from a deep-sea-derived bacterium Chromobacterium sp. HS-13-94. Its structure was determined by extensive spectroscopic analysis and by comparison with a relat...A bicyclic depsipeptide, chromopeptide A(1), was isolated from a deep-sea-derived bacterium Chromobacterium sp. HS-13-94. Its structure was determined by extensive spectroscopic analysis and by comparison with a related known compound. The absolute configuration of chromopeptide A was established by X-ray diffraction analysis employing graphite monochromated Mo K_α radiation(λ ? 0.71073 ?) with small Flack parameter 0.03. Chromopeptide A suppressed the proliferation of HL-60, K-562, and Ramos cells with average IC_(50) values of 7.7, 7.0, and 16.5 nmol/L, respectively.展开更多
New marine bacterium Zooshikella sp.SY01,producer of prodigiosin,was isolated from the seawaters of Sanya Bay.The culture conditions of this bacterium were investigated.Zooshikella sp.SY01 was cultured in 2216E media ...New marine bacterium Zooshikella sp.SY01,producer of prodigiosin,was isolated from the seawaters of Sanya Bay.The culture conditions of this bacterium were investigated.Zooshikella sp.SY01 was cultured in 2216E media which contained tryptophan,histidine,lac-tonic acid,camphor,limonene,casein,diphenyl guani-dine,coumarin and 1,3-dinitrobenzene,respectively.After 5 days cultivation,the extracts of different culture broths were detected by direct infusion mass spectroscopy using positive ESI mode.As the results,tryptophan,his-tidine and casein didn't show any observable influences on the biosynthesis of prodigiosin.Lactonic acid,camphor,limonene,diphenyl guanidine,coumarin could inhibit the bacterium growth and prodigiosin biosynthesis to a cer-tain extent,slower the culture broth to turn red.However,1,3-dinitrobenzene inhibited the bacteria to produce pro-digiosin completely.MS data suggested that various metabolites with chemodiversity were produced in differ-ent culture media.In particular,a series of high-molecu-lar-weight compounds with high relative abundances were observed in the medium containing limonene.To further optimize the culture condition,more new prodigiosin ana-logues and lead compounds can be obtained and the goal of"one strain-many compounds"can be achieved.展开更多
基金supported by National Basic Research Program of China(973 program,Grant No.2009CB724700)the Hundred Talent Program of the Chinese Academy of Sciences(A1097)National Natural Science Foundation of China(No.31100092)
文摘2-haloacid dehalogenases constitute a group of dehalogenases which are capable of dehalogenating the halogenated organic compounds. So far, the 2-haloacid dehalogenases have been found in many bacteria, but not in Paracoccus genus. In the present study, one enzyme 2-haloacid dehalogenase(designated as Deh99), induced by DL-2-chloropropionate(DL-2-CPA), was purified from the marine bacterium Paracoccus sp. DEH99, isolated from marine sponge Hymeniacidon perlevis. The enzyme of Deh99 was purified to homogeneity by ammonium sulfate precipitation, ion exchange chromatography(Q-Sepharose HP), and Superdex 200 gel filtration chromatography. The molecular weight of Deh99 was estimated to be 25.0 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE), and 50.0 kDa natively by gel filtration chromatography. The enzyme of Deh99 stereospecifically dehalogenated L-2-CPA to produce D-lactate, with an apparent Michaelis-Menten constant(Km) value of 0.21 mmol L-1 for L-2-CPA. The optimal pH and temperature for Deh99 activity were 10.0 and 40℃, respectively. The enzyme of Deh99 acted on short-carbon-chain 2-haloacids, with the highest activity towards monochloroacetate. The activity of Deh99 was slightly affected by DTT and EDTA, but strongly inhibited by Cu2+ and Zn2+. The enzyme of Deh99 shows unique substrate specificity and inhibitor sensitivities compared to previously characterized 2-haloacid dehalogenases and is the reported one about purified 2-haloacid dehalogenase isolated from the bacteria of Paracoccus genus.
基金Supported by the fund"The Documentation Concordancy and Sharing of Germplasm Resources of Marine Microorganism Resources at Offshore China"from Science and Technology Ministry(2005DKA21209)~~
文摘[Objective] The aim of this study is to identify a bacterial strain isolated from ocean water from the Yellow Sea.[Method]Using 16S rRNA technique,a strain from Yellow Sea was preliminarily identified and analyzed.[Result]One 1 521 bp fragment of 16S rRNA was amplified from the strain HZBN43;homology analysis between the yielded sequence and the 16S rRNA sequences accessed in NCBI from other strains showed that HZBN43 belonged to Bacillus,and shared 99.79% homologue with the known species of Bacillus selenatarsenatis.[Conclusion]The sequence of strain HZBN43 was obtained.However,because of the incomplete sequence,the confidence level is just 46,so other corroborations are still required for grouping HZBN43 into an exact species.
文摘采用多种色谱分离技术,从南海深海细菌Bacillus amyloliquefaciens GAS 00152发酵液中分离得到12个化合物,经波谱数据分析分别鉴定为4-苯基-3-丁烯酰胺（1）、苯乙酰胺（2）、苯乙酸-2-（4-羟苯基）乙酯（3）、对羟基苯乙醇（4）、环（甘氨酸-2-氨基丁酸）（5）、环（甘氨酸-亮氨酸）（6）、环（甘氨酸-L-脯氨酸）（7）、环（D-脯氨酸-L-缬氨酸）（8）、环（4-羟基脯氨酸-亮氨酸）（9）、环（N-甲基甘氨酸-苯丙氨酸）（10）、环（L-2-羟基脯氨酸-苯丙氨酸）（11）、环（2-哌啶酸-苯丙氨酸）（12）。其中,化合物1为新天然产物。测试化合物1-12对番木瓜炭疽菌和香蕉黑星菌的抑制活性,发现化合物9对两种热带水果致病菌显示出中等抗菌活性。
基金financially supported by the National Marine ‘863’ Project (Nos. 2012AA092105 and 2013AA092902)the National Natural Science Foundation of China (No. 81273430)
文摘A bicyclic depsipeptide, chromopeptide A(1), was isolated from a deep-sea-derived bacterium Chromobacterium sp. HS-13-94. Its structure was determined by extensive spectroscopic analysis and by comparison with a related known compound. The absolute configuration of chromopeptide A was established by X-ray diffraction analysis employing graphite monochromated Mo K_α radiation(λ ? 0.71073 ?) with small Flack parameter 0.03. Chromopeptide A suppressed the proliferation of HL-60, K-562, and Ramos cells with average IC_(50) values of 7.7, 7.0, and 16.5 nmol/L, respectively.
基金supported by the National Natural Science Foundation of China(Grant Nos.20502036,20602044)the Natural Science Foundation of Guangdong Province(05300667)the Young Teacher Research Foundation of Sun Yat-sen University(2004-36000-1131072).
文摘New marine bacterium Zooshikella sp.SY01,producer of prodigiosin,was isolated from the seawaters of Sanya Bay.The culture conditions of this bacterium were investigated.Zooshikella sp.SY01 was cultured in 2216E media which contained tryptophan,histidine,lac-tonic acid,camphor,limonene,casein,diphenyl guani-dine,coumarin and 1,3-dinitrobenzene,respectively.After 5 days cultivation,the extracts of different culture broths were detected by direct infusion mass spectroscopy using positive ESI mode.As the results,tryptophan,his-tidine and casein didn't show any observable influences on the biosynthesis of prodigiosin.Lactonic acid,camphor,limonene,diphenyl guanidine,coumarin could inhibit the bacterium growth and prodigiosin biosynthesis to a cer-tain extent,slower the culture broth to turn red.However,1,3-dinitrobenzene inhibited the bacteria to produce pro-digiosin completely.MS data suggested that various metabolites with chemodiversity were produced in differ-ent culture media.In particular,a series of high-molecu-lar-weight compounds with high relative abundances were observed in the medium containing limonene.To further optimize the culture condition,more new prodigiosin ana-logues and lead compounds can be obtained and the goal of"one strain-many compounds"can be achieved.
