Transport and behavior of marine oil spill containing polycyclic aromatic hydrocarbons in mesocosm experiments

Polycyclic aromatic hydrocarbons(PAHs)are one of the most important groups in oil,and re sponsible for major toxic and/or carcinogenic impact on humans and wildlife.It is important to understand the behavior of PAHs in marine environment after an oil-spill incident.However,interaction between petroleum PAHs and microbial communities in a marine environment remains unclear.Therefore,a series of mesocosm experiments were conducted.in which water-accommodated fraction(WAF)of oil was generated to simulate an oil-spill scenario and to analyze the transport and behavior of marine oil spill containing PAHs with and without dispersants.Results indicate that the application of dispersant could increase the concentration of total PAHs in water column due mainly to significant increase in the concentration of highmolecular weight(HMW)PAHs at a lower removal rate.At the end of the 7-day experiment,significant amount of HMW PAHs were accumulated in sediment.In general,the application of dispersant did not increase the sediment uptake of PAHs but increased the PAHs concentration in water column.

A set of source-and age-diagnostic biomarkers discovered in the marine oils

1 Introduction Biological markers or biomarkers(Eglinton et al.,1964;Eglinton and Calvin,1967)are molecular fossils,which were derived from once living organisms through the earth history.They occur in sediments,rocks,and crude

Extended hopanes and 8,14-secohopanes up to C_(40)in unbiodegraded marine oils from the Tarim Basin,NW China

Extended 17α(H),21β(H)-hopanes and three series of 8,14-secohopanes up to C_(40),including 8α(H),14α(H),17α(H),21β(H)-,8α(H),14α(H),17β(H),21α(H)-and 8α(H),14β(H),17β(H),21α(H)-,were detected by GC-MS-MS method in the branched/cyclic hydrocarbon fractions of some unbiodegraded marine oils from the Tazhong uplift in the Tarim Basin,NW China.The coexistence of extended hopanes and 8,14-secohopanes up to C_(40)in unbiodegraded oils suggests that they are primary and independent on biodegradation.The similarity of distribution and composition for extended hopanes and 8,14-secohopanes up to C_(40)in unbiodegraded oils proposes that they could be derived from a similar biological precursor.However,an abrupt decrease up to 3-5 times in the relative abundance from C_(35)to C_(36)in C_(31-40)extended hopanes and extended 8,14-secohopanes suggests that C_(31-35)and C_(36-40)extended hopanes and extended 8,14-secohopanes should have their own biological precursor.The known C35bacteriohopanetetrol should be biological precursor of C_(31-35)extended hopanes and 8,14-secohopanes,but an unknown C_(40)functionalized hopanoid could be biological precursor of C_(36-40)extended hopanes and 8,14-secohopanes.More attention should be paid to their potential roles in oil-source correlation for severely biodegraded oils based on their widespread occurrence in various source rocks,unbiodegraded and severely biodegraded oils.

3D Slicing of Weighted Index for Reservoir Quality of Lower Miocene Rocks, Belayim Marine Oil Field, Gulf of Suez, Egypt

This study aims to have a single coefficient resulting from the integration of all the reservoir parameters through which a decision can be taken to determine the best quality places in the reservoir. The conventional well logging data in nine wells were used to determine the reservoir parameters in the study area. Seven different parameters were calculated, five of them were directly proportional to the quality of the reservoir, while the remaining two parameters which represent shale volume and water saturation were inversely proportional to the reservoir quality. The index of each parameter was calculated. A new value was created from the integration of the seven different parameter indexes called the weighted index for the reservoir quality. The reservoir quality values were sliced in the three dimensions depending on the effect of all reservoir parameters and not on any single parameter. It is clear from the results of this study that horizontal and vertical slicing, as well as cut-off values, illustrates that the middle and upper parts are the best places for the reservoir to explore hydrocarbons, where the values of the weighted index of the reservoir quality range from 0.65 to 0.9. Meanwhile, the quality of the reservoir decreases in its lower parts.

Characteristics and discovery significance of the Upper Triassic–Lower Jurassic marine shale oil in Qiangtang Basin,China

Mesozoic marine shale oil was found in the Qiangtang Basin by a large number of hydrocarbon geological surveys and shallow drilling sampling.Based on systematic observation and experimental analysis of outcrop and core samples,the deposition and development conditions and characteristics of marine shale are revealed,the geochemical and reservoir characteristics of marine shale are evaluated,and the layers of marine shale oil in the Mesozoic are determined.The following geological understandings are obtained.First,there are two sets of marine organic-rich shales,the Lower Jurassic Quse Formation and the Upper Triassic Bagong Formation,in the Qiangtang Basin.They are mainly composed of laminated shale with massive mudstone.The laminated organic-rich shale of the Quse Formation is located in the lower part of the stratum,with a thickness of 50–75 m,and mainly distributed in southern Qiangtang Basin and the central-west of northern Qiangtang Basin.The laminated organic-rich shale of the Bagong Formation is located in the middle of the stratum,with a thickness of 250–350 m,and distributed in both northern and southern Qiangtang Basin.Second,the two sets of laminated organic-rich shales develop foliation,and various types of micropores and microfractures.The average content of brittle minerals is 70%,implying a high fracturability.The average porosity is 5.89%,indicating good reservoir physical properties to the level of moderate–good shale oil reservoirs.Third,the organic-rich shale of the Quse Formation contains organic matters of types II1 and II2,with the average TOC of 8.34%,the average content of chloroform bitumen'A'of 0.66%,the average residual hydrocarbon generation potential(S1+S2)of 29.93 mg/g,and the Ro value of 0.9%–1.3%,meeting the standard of high-quality source rock.The organic-rich shale of the Bagong Formation contains mixed organic matters,with the TOC of 0.65%–3.10%and the Ro value of 1.17%–1.59%,meeting the standard of moderate source rock.Fourth,four shallow wells(depth of 50–250 m)with oil shows have been found in the organic shales at 50–90 m in the lower part of the Bagong Formation and 30–75 m in the middle part of the Quse Formation.The crude oil contains a high content of saturated hydrocarbon.Analysis and testing of outcrop and shallow well samples confirm the presence of marine shale oil in the Bagong Formation and the Quse Formation.Good shale oil intervals in the Bagong Formation are observed in layers 18–20 in the lower part of the section,where the shales with(S0+S1)higher than 1 mg/g are 206.7 m thick,with the maximum and average(S0+S1)of 1.92 mg/g and 1.81 mg/g,respectively.Good shale oil intervals in the Quse Formation are found in layers 4–8 in the lower part of the section,where the shales with(S0+S1)higher than 1 mg/g are 58.8 m thick,with the maximum and average(S0+S1)of 6.46 mg/g and 2.23 mg/g,respectively.

Isolation and characterization of a high-efficiency marine diesel oil-degrading bacterium

In the recent 50 years,marine oil spills had resulted in severe environmental pollution problems worldwide.In this study,12 petroleum-degrading strains named MJ1 to MJ12,which can use diesel oil as the sole carbon source for growth,were isolated from the seawater in Jiaozhou Bay,China.Strain MJ4 has the highest diesel-degrading rate which is up to 26.54%in 5 days with the diesel oil concentration of 10 g/L.According to the BLAST research,16SrRNA sequence of MJ4 showed 99%similarity to Bacillus megaterium strain.Single-factor experiments and response surface methodology were carried out to optimize the environmental factors and their reciprocal action for affecting the diesel oil degradation process of Bacillus sp.MJ4.Results of single-factor experiments revealed that the highest degradation rate was obtained with temperature of 28°C,pH of 8.8,diesel oil concentration of 25 g/L,P/N ratio of 0.56,nitrogen and phosphorus dosage quantity of 0.35 g/L and 0.18 g/L,respectively.A nonlinear regression equation of diesel oil degradation rate and pH,temperature,P/N ratio was obtained.The model predicted the maximum degradation rate of 72.21%with temperature of 28°C,pH of 8.88,P/N ratio of 0.31,respectively.

Geochemical significances of 8,14-secohopanes in marine crude oils from the Tazhong area in the Tarim Basin,NW China

8,14-secohopanes in the marine oils from the Tazhong area in the Tarim Basin are detected by gas chromatography-mass spectrometry(GC-MS)and gas chromatography-mass spectrometry-mass spectrometry(GC-MS-MS),and their distributions and compositions are compared in order to study their potential significances in oil-source correlation.C35+extended hopane series and three series of extended 8,14-secohopanes can be detected in two kinds of end-member oils in the Tazhong area in the Tarim Basin,and they are different in distribution,suggesting that they may have some special geochemical significance.The presence of 8,14-secohopanes in two kinds of end-member oils in the Tarim Basin suggests that these biomarkers are primary,and not related to biodegradation.The relative abundance of 8,14-secohopanes in the type-A oil is much less than that in the type-B oil,and the 8,14-secohopanes content in end-member oils is much less than that in the corresponding mixed oils.Based on the relative contents of 8,14-secohopanes and the compositions of common steranes and triterpanes,it is very effective to distinguish different crude oils from the Tazhong area.The great difference in the relative abundance of 8,14-secohopanes between the type-A oil and type-B oil suggests that their formation may require some specific geological-geochemical conditions.

Geochemical characterization of aromatic hydrocarbons in crude oils from the Tarim,Qaidam and Turpan Basins,NW China

Based on the systematic study of aromatic hydrocarbons in over 100 crude oil samples collected from the Tabei and Tazhong uplifts in the Tarim Basin,the western depression area in the Qaidam Basin and the Tabei depression in the Turpan Basin,the geochemical characteristics of the marine（Tarim Basin）,saline lacustrine（Qaidam Basin）,and swamp（Turpan Basin） oils were investigated.The marine oils from the Tarim basin are characterized by relatively low abundance of diaromatic hydrocarbons such as biphenyl and naphthalene,and relatively high abundance of triaromatic hydrocarbons including phenanthrene,dibenzothiophene and fluorene.In contrast,the swamp oils from the Turpan Basin are dominated by the highest relative abundance of diaromatic hydrocarbons and the lowest relative abundance of triaromatic hydrocarbons in all the oil samples in this study.The relative abundance of diaromatic and triaromatic hydrocarbons in the saline lacustrine oils from Qaidam Basin is between that in Tarim oils and Turpan oils.Aromatic parameters based on the isomer distributions of dimethylnaphthalenes（DMN）,trimethylnaphthalenes（TMN）,tetramethylnaphthalenes（TeMN） and methylphenanthrenes（MP）,i.e.,1,2,5-trimethylnaphthalene（TMN）/1,3,6-TMN ratio,1,2,7-TMN/1,3,7TMN ratio,（2,6-＋2,7-）-dimethylnaphthalenes（DMN）/1,6-DMN ratio,1,3,7-TMN/（1,2,5-＋1,3,7-）TMN,1,3,6,7-TeMN/（1,3,6,7-＋1,2,5,6-＋1,2,3,5-）-TeMN ratio and MP index,may reflect the diversity of organic source input,thermal maturity and depositional environments.In addition,the dibenzothiophenes（DBTs）/fluorenes（Fs） and dibenzofurans（DBFs）/Fs ratios were found to the very useful and effective in determining genetic types of crude oils for the marine,saline lacustrine,and swamp depositional environments,and for oil-oil correlations.

A fast,edge-preserving,distance-regularized model with bilateral filtering for oil spill segmentation of SAR images

Marine oil spills are among the most significant sources of marine pollution.Synthetic aperture radar(SAR)has been used to improve oil spill observations because of its advantages in oil spill detection and identification.However,speckle noise,weak boundaries,and intensity inhomogeneity often exist in the oil spill regions of SAR imagery,which will seriously aff ect the accurate identification of oil spills.To enhance marine oil spill segmentation of SAR images,a fast,edge-preserving framework based on the distance-regularized level set evolution(DRLSE)model was proposed.Specifically,a bilateral filter penalty term is designed and incorporated into the DRLSE energy function(BF-DRLSE)to preserve the edges of oil spills,and an adaptive initial box boundary was selected for the DRLSE model to reduce the operation time complexity.Two sets of RadarSat-2 SAR data were used to test the proposed method.The experimental results indicate that the bilateral filtering scheme incorporated into the energy function during level set evolution improved the stability of level set evolution.Compared with other methods,the proposed improved BF-DRLSE algorithm displayed a higher overall segmentation accuracy(97.83%).In addition,using an appropriate initial box boundary for the DRLSE method accelerated the global search process,improved the accuracy of oil spill segmentation,and reduced computational time.Therefore,the results suggest that the proposed framework is eff ective and applicable for marine oil spill segmentation.

Radarsat observations and forecasting of oil slick trajectory movements

RADARSAT data have a potential role for coastal pollution monitoring. This study presents a new approach to detect and forecast oil slick trajectory movements. The oil slick trajectory movements is based on the tidal current effects and Fay's algorithm for oil slick spreading mechanisms. The oil spill trajectory model contains the integration between Doppler frequency shift model and Lagrangian model. Doppler frequency shift model implemented to simulate tidal current pattern from RADARSAT data while the Lagrangian model used to predict oil spill spreading pattern. The classical Fay's algorithm was implemented with the two models to simulate the oil spill trajectory movements.The study shows that the slick lengths are effected by tidal current V component with maximum velocity of 1.4 m/s. This indicates that oil slick trajectory path is moved towards the north direction. The oil slick parcels are accumulated along the coastline after 48 h. The analysis indicated that tidal current V components were the dominant forcing for oil slick spreading.

Petroleum geology of marl in Triassic Leikoupo Formation and discovery significance of Well Chongtan1 in central Sichuan Basin,SW China

In 2022,the risk exploration well Chongtan1(CT1)in the Sichuan Basin revealed commercial oil and gas flow during test in a new zone–the marl of the second submember of the third member of Leikoupo Formation(Lei-32)of Middle Triassic,recording a significant discovery.However,the hydrocarbon accumulation in marl remains unclear,which restricts the selection and deployment of exploration area.Focusing on Well CT1,the hydrocarbon accumulation characteristics of Lei-32 marl are analyzed to clarify the potential zones for exploration.The following findings are obtained.First,according to the geochemical analysis of petroleum and source rocks,oil and gas in the Lei-32 marl of Well CT1 are originated from the same marl.The marl acts as both source rock and reservoir rock.Second,the Lei-32 marl in central Sichuan Basin is of lagoonal facies,with a thickness of 40–130 m,an area of about 40000 km^(2),a hydrocarbon generation intensity of(4–12)×10^(8) m^(3)/km^(2),and an estimated quantity of generated hydrocarbons of 25×10^(12) m^(3).Third,the lagoonal marl reservoirs are widely distributed in central Sichuan Basin.Typically,in Xichong–Yilong,Ziyang–Jianyang and Moxi South,the reservoirs are 20–60 m thick and cover an area of 7500 km^(2).Fourth,hydrocarbons in the lagoonal marl are generated and stored in the Lei-32 marl,which means that marl serves as both source rock and reservoir rock.They represent a new type of unconventional resource,which is worthy of exploring.Fifth,based on the interpretation of 2D and 3D seismic data from central Sichuan Basin,Xichong and Suining are defined as favorable prospects with estimated resources of(2000–3000)×10^(8) m^(3).

Petroleum Exploration of Craton Basins in China

Craton basins are a significant petroliferous provenance. Having undergone multiple open- dose tectonic cycles and strong reworking of the late Cenozoic tectonic movement, the craton basins in China are highly broken. This has resulted in multi-source and multiphase hydrocarbon generation and later hydrocarbon accumulation so that a complicated spatial assemblage of primary, paraprimary and secondary oil-gas pools has been formed. The primary factors controlling hydrocarbon accumulation include hydrocarbon-generating depressions, paleouplifts, paleoslopes, unconformity surfaces, paleo-karst, faults and fissure systems as well as the later conservation conditions. In consequence, the strategy of exploration for China＇s craton basins is to identify the effective source rocks, pay attention to the different effects of paleohighs and late reworking, enhance studies of the secondary storage space, attach importance to the exploration of lithologic oil-gas reservoirs and natural gas pools, and approach consciously from the secondary oil pools to the targets near the source rocks. At the same time, a complete system of technologies and techniques must be built up.

Structure and Potential Application of Konjac Glucomannan Nano Microfibril Aerogel

An ultra-light and high porosity nano microfibril aerogel was prepared from konjac glucomannan（KGM） by the electrospinning and freeze-drying. The structure of aerogel was analyzed by scanning electron microscopy（SEM） and X-ray diffraction（XRD） while the density and compressive strength of the samples were studied separately. Results reveal that porous network structure of the KGM nano microfibril aerogel is constructed by intermolecular hydrogen bonds in random and interpenetrate way. The nano microfibril structure presents in the KGM aerogel,which is an important reason of its high density and compressive strength. There is a potential application for this unique nano microfibril aerogel in the absorption of biodegradation bacteria to solve problems in marine oil spill pollution.

基于船舶自动识别系统(AIS)大数据的海上石油泄漏事故敏感源与交通密度风险分析

This study developed a new methodology for analyzing the risk level of marine spill accidents from two perspectives,namely,marine traffic density and sensitive resources.Through a case study conducted in Busan,South Korea,detailed procedures of the methodology were proposed and its scalability was confirmed.To analyze the risk from a more detailed and microscopic viewpoint,vessel routes as hazard sources were delineated on the basis of automated identification system(AIS)big data.The outliers and errors of AIS big data were removed using the density-based spatial clustering of applications with noise algorithm,and a marine traffic density map was evaluated by combining all of the gridded routes.Vulnerability of marine environment was identified on the basis of the sensitive resource map constructed by the Korea Coast Guard in a similar manner to the National Oceanic and Atmospheric Administration environmental sensitivity index approach.In this study,aquaculture sites,water intake facilities of power plants,and beach/resort areas were selected as representative indicators for each category.The vulnerability values of neighboring cells decreased according to the Euclidean distance from the resource cells.Two resulting maps were aggregated to construct a final sensitive resource and traffic density(SRTD)risk analysis map of the Busan–Ulsan sea areas.We confirmed the effectiveness of SRTD risk analysis by comparing it with the actual marine spill accident records.Results show that all of the marine spill accidents in 2018 occurred within 2 km of high-risk cells(level 6 and above).Thus,if accident management and monitoring capabilities are concentrated on high-risk cells,which account for only 6.45%of the total study area,then it is expected that it will be possible to cope with most marine spill accidents effectively.

Emissions of intermediate volatility organic compound from waste cooking oil biodiesel and marine gas oil on a ship auxiliary engine

Ship auxiliary engines contribute large amounts of air pollutants when at berth.Biodiesel,including that from waste cooking oil(WCO),can favor a reduction in the emission of primary pollutant when used with internal combustion engines.This study investigated the emissions of gaseous intermediate-volatile organic compounds(IVOCs)between WCO biodiesel and marine gas oil(MGO)to further understand the differences in secondary organic aerosol(SOA)production of exhausts.Results revealed that WCO exhaust exhibited similar IVOC composition and volatility distribution to MGO exhaust,despite the differences between fuel contents.While WCO biodiesel could reduce IVOC emissions by 50%as compared to MGO,and thus reduced the SOA production from IVOCs.The compositions and volatility distributions of exhaust IVOCs varied to those of their fuels,implying that fuel-component-based SOA predicting model should be used with more cautions when assessing SOA production of WCO and MGO exhausts.WCO biodiesel is a cleaner fuel comparing to conventional MGO on ship auxiliary engines with regard to the reductions in gaseous IVOC emissions and corresponding SOA productions.Although the tests were conducted on test bench,the results could be considered as representative due to the widely applications of the test engine and MGO fuel on real-world ships.

Enhancing Coordination for Effective Management of Oil Spill Pollution in South Africa

Although multi-sectoral coordination in disaster risk management has been progressing in South Africa for over two decades,there has been limited commitment to an integrated regime in managing marine oil spill incidents.Poor incident management persists despite the availability of data,protocols,legislation,and resources housed in different government and private sector entities.This study identified practices that enhance a coordination process for the effective management of oil spill pollution.A grounded theory approach is applied to the coordination issue,which is characterized by an interactive process of simultaneously considering theoretical grounding during our empirical research.Empirical evidence includes observations of 47meetings and three oil spill exercises with 79 delegates from 32 different organizations,which supports the coordination process of instituting a national Incident Management System for marine oil spills in South Africa.An additional 44 individual open-ended questionnaires supplement this earlier body of evidence for data triangulation and validation.Analysis of development of the Incident Management System process revealed that,when designing a novel long-term project that is reliant on a shared vision from multiple organizations,enhanced coordination and collaboration for successful implementation is dependent on the following practices:(1)obtaining political commitment,(2)bridging knowledge gaps,and(3)sharing resources.

Comparison the effects of bioaugmentation versus biostimulation on marine microbial community by PCR–DGGE： A mesocosm scale

In order to better understand the effects of biostimulation and bioaugmentation processes on a marine microbial community, three different mesocosm experiments were planned. Natural seawater（10.000 L） was artificially polluted with crude oil（1 L） and（1） inorganic nutrients（Biostimulating Mesocosm, BM）,（2） inorganic nutrients and an inoculum of Alcanivorax borkumensis SK2（Single Bioaugmentation Mesocosm, SBM）,（3） inorganic nutrients and inoculums of A. borkumensis SK2 and Thalassolituus oleivorans MIL-1（Consortium Bioaugmentation Mesocosm, CBM）. During the experimental period（20 days）, samples were taken from each mesocosm and the community structure was analyzed by PCR–DGGE. The 16 S r RNA gene DGGE banding patterns and sequence analysis demonstrated that biostimulation had the lowest effect on microbial biodiversity in the mesocosms; however, the biodiversity of the marine microbial community dramatically decreased in the CBM（Shannon index was 0.6 in T3）. The community structures among the three mesocosms were also markedly different,and major bacteria derived from DGGE bands were related to uncultured Gamma Proteobacteria. The biodegradation results show that the Single Bioaugmentation Mesocosm（SBM） system had the highest percentage of degradation（95%） in comparison to the BM mesocosm（80%） and CBM（70%）.