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Bioactive chemical constituents from the marine-derived fungus Cladosporium sp.DLT-5
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作者 Luting DAI Qingyi XIE +6 位作者 Jiaocen GUO Qingyun MA Li YANG Jingzhe YUAN Haofu DAI Zhifang YU Youxing ZHAO 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2024年第3期905-914,共10页
A new isochromanone,cladosporinisochromanone(1),accompanied by 15 known compounds(2–16)were obtained from secondary metabolites produced by marine-derived fungus Cladosporium sp.DLT-5.NMR and HRESIMS spectra elucidat... A new isochromanone,cladosporinisochromanone(1),accompanied by 15 known compounds(2–16)were obtained from secondary metabolites produced by marine-derived fungus Cladosporium sp.DLT-5.NMR and HRESIMS spectra elucidation determined the planar structure of 1.Subsequent electronic circular dichroism(ECD)experiment assigned the absolute configuration of 1.Compounds 1,2,4–6,and 10 displayed different degrees of neuroprotective activities on human neuroblastoma cells SH-SY5Y.Five compounds(1,3–5,and 13)emerged resistance to protein tyrosine phosphatase 1B(PTP1B),further kinetic analysis and molecular docking study indicated that the most potent compound 13(IC50value of 10.74±0.61μmol/L)was found as a noncompetitive inhibitor for PTP1B.Surface plasmon resonance(SPR)and molecular docking studies also demonstrated the interaction between compound 12 and Niemann-Pick C1 Like 1(NPC1L1),which has been identified as significant therapeutic target for hypercholesteremia.In addition,compounds 3,6,and 14 showed attractive inhibitory activity against the phytopathogenic fungi:Colletotrichum capsici.Therefore,library of Cladosporium metabolites is enriched and new active uses of known compounds are explored. 展开更多
关键词 Cladosporium sp. marine-derived fungus neuroprotective effects protein tyrosine phosphatase 1B(PTP1B) Niemann-Pick C1 Like 1(NPC1L1) antifungal activity
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基于Actinomyces ruminicola的微态制剂的体外发酵效果
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作者 白云龙 肖鑫焕 +3 位作者 胡盼 吴凌 夏成 张洪友 《黑龙江畜牧兽医》 CAS 北大核心 2016年第11期120-123,共4页
为了研制一种新的微生态制剂,试验利用分离得到的瘤胃厌氧菌Actinomyces ruminicola(命名为NY2)与痤疮丙酸杆菌混合制成微生态制剂进行体外发酵,研究微生态制剂的体外发酵特性及产挥发性脂肪酸的能力,确定微生态制剂中Actinomyces rumin... 为了研制一种新的微生态制剂,试验利用分离得到的瘤胃厌氧菌Actinomyces ruminicola(命名为NY2)与痤疮丙酸杆菌混合制成微生态制剂进行体外发酵,研究微生态制剂的体外发酵特性及产挥发性脂肪酸的能力,确定微生态制剂中Actinomyces ruminicola与痤疮丙酸杆菌的最佳混合比例。结果表明:当微生态制剂中Actinomyces ruminicola与痤疮丙酸杆菌混合比为1∶1时,培养瓶中乙酸、丙酸、丁酸和总挥发性脂肪酸的浓度最高值分别达到91.07,41.12,27.58,169.19 mmol/L。该比例微生态制剂可保持瘤胃内容物pH值在6.0左右波动,有利于瘤胃微生物发酵,并能促进乙酸、丙酸等挥发性脂肪酸的生成。 展开更多
关键词 actinomyces ruminicola 微生态制剂 体外发酵特性 挥发性脂肪酸 瘤胃微生物
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Separation and Screening of Biocontrol Actinomyces against Alfalfa Root Rot
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作者 王兰英 徐林波 +1 位作者 何勇 乌兰巴特尔 《Plant Diseases and Pests》 CAS 2010年第2期10-12,37,共4页
[Objective] Actinomycetes with high antagonistic effects on alfalfa root rot pathogen was isolated from 10 soil samples in Chifeng Inner Mongolia. [Method] 91 actinomyces were separated from 10 soil samples in Chifeng... [Objective] Actinomycetes with high antagonistic effects on alfalfa root rot pathogen was isolated from 10 soil samples in Chifeng Inner Mongolia. [Method] 91 actinomyces were separated from 10 soil samples in Chifeng Inner Mongolia by gradient dilution separation method. In duel culture tests, all isolates were tested for their antagonism by using 3 strains including Fusarium solani, F. oxysoporum, F. avenaceum of alfalfa root rot pathogenic bacteria as indicator strains. [Result] 5 strains with strong antagonistic effect on tested alfalfa root rot pathogen were obtained from No.1, No.4, No.6 and No.7 soil samples which were numbered 1-3-6, 4-4-2, 6-2-27 and 7-2-13 respectively, accounted for 5.50% in separated strains. [Conclusion] This study laid certain foundation for biological control of alfalfa root rot disease. 展开更多
关键词 actinomyces Antagonistic effect ALFALFA Root rot
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The Antitumor Components from Marine-derived Bacterium Streptoverticillium luteoverticillatum 11014 Ⅱ 被引量:4
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作者 LI Dehai ZHU Tianjiao FANG Yuchun LIU Hongbing GU Qianqun ZHU Weiming 《Journal of Ocean University of China》 SCIE CAS 2007年第2期193-195,共3页
Eight known compounds were isolated from a marine-derived bacterium Streptoverticillium luteoverticillatum 11014 using bioassay-guided fractionations. Their structures were identified by spectral analysis as bis (4-h... Eight known compounds were isolated from a marine-derived bacterium Streptoverticillium luteoverticillatum 11014 using bioassay-guided fractionations. Their structures were identified by spectral analysis as bis (4-hydroxybenzyl) ether (1), p-hydroxyphenylethyl alcohol (2), N-(4-hydroxyphenethyl) acetamide (3), indole-3 carboxylic acid methyl ester (4), dibenzo[b,e] [1,4]dioxine (5), thymine (6), cytosine deoxyribonucleoside (7) and 2, 3-butanediol (8). These compounds were evaluated for their cytotoxic activities against K562 cell line with the SRB method for the first time. Compounds 2 and 4 showed cytotoxcities with IC50 values of 101.1 and 165.3 μolL^-1, respectively. All compounds were isolated from S. luteoverticillatum 11014 for the first time. 展开更多
关键词 marine-derived bacterium Streptoverticillium luteoverticillatum antitumor activity
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The Cytotoxic Constituents from Marine-derived Streptomyces 3320^(#) 被引量:4
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作者 REN Hong GU Qianqun +1 位作者 CUI Chengbin ZHU Weiming 《Journal of Ocean University of China》 SCIE CAS 2006年第1期75-81,共7页
The present work studies the chemical constituents from marine-derived streptomyces 3320^# and their antitumor activities. The n-BuOH extract of the ferment broth of 3320^# was chromatographed on silica gel, Sephadex ... The present work studies the chemical constituents from marine-derived streptomyces 3320^# and their antitumor activities. The n-BuOH extract of the ferment broth of 3320^# was chromatographed on silica gel, Sephadex LH-20, ODS columns and HPLC to separate the compounds with antitoumor activities. Their structures were identified using IR, UV, NMR, MS spectroscopic techniques and compared with published data. The antitumor activities of the isolates were assayed using SRB method and flow cytometry assay, accompanied with the morphological observation of the cells under light micro- scope against mammalian tsFT210 cells. Ten compounds, cyclo-(Ala-Leu) 1, cyclo-(Ala-Ile) 2, cyclo-(Ala-Val) 3, cyclo- (Phe-Pro) 4, cyclo-(Phe-Gly)5, cyclo-(Leu-Pro)6, 1-methyl-1, 2, 3, 4-tetrahydro-β-carboline-3-carboxylic acid 7, N-(4- hydroxyphenethyl) acetamide 8, 4-methyoxy-l-(2-hydroxy) ethylbenzene 9 and uridine 10, were isolated from the ferment broth of streptomyces 3320^# . Among them, compounds 6, 7, 8 and 10 showed potent cytotoxicity against the tsFT210 cell with the IC50 values of 3.6, 7.2, 5.2 and 1.6 mmol L-1, respectively. Compounds 8, 10 also exhibited apoptosis inducing activity under 2.0 mmol L-1. Compounds 6, 7, 8 and 10 are the principle bioactive constituents responsible for the antitumor activities of marine streptomyces 3320^#. Compound 7 was isolated from this species for the first time. 展开更多
关键词 marine-derived streptomyces secondary metabolites structural identify bioassay-guided fractionation antitumor activity
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High Ethanol Production by Marine-Derived Yeasts-<i>Saccharomyces cerevisiae</i>under Stress Pressures 被引量:1
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作者 Naoto Urano Ayaka Shirao +1 位作者 Masahiko Okai Masami Ishida 《Advances in Microbiology》 2017年第5期349-357,共9页
For practical applications of bioethanol, the uses of both highly concentrated biomass materials and their effective fermentation by yeasts are indispensable in order to produce ethanol at low costs. However, as the s... For practical applications of bioethanol, the uses of both highly concentrated biomass materials and their effective fermentation by yeasts are indispensable in order to produce ethanol at low costs. However, as the saccharified products of those biomass generally contain abundant sugars, the yeasts are affected by the compounds and are inclined to decrease their physiological activities. In the process of fermentation, ethanol is gradually produced by the yeasts in the culture;the concentrated metabolic product also damages itself, and inhibition of the fermentation frequently occurs. The application of yeasts with high fermentative activities under stress pressures such as sugars and ethanol is thus desired for bioethanol production. In this study, various types of high-fermentative yeasts under stress pressures were isolated mainly from coastal waters in Japan and characterized. All yeast strains with high fermentative activities under 20% v/v ethanol were found to be Saccharomyces cerevisiae. The HK21 strain isolated from Tokyo Bay and identified as S. cerevisiae had the highest fermentation activity under 30% w/v sorbitol and under 20% v/v ethanol, and it produced approx. 70 g/l (9% v/v) ethanol from the 15% w/v glucose solution at 25 oC within 5 days. 展开更多
关键词 marine-derived YEAST Fermentation Bioethanol Coastal Water SACCHAROMYCES CEREVISIAE Stress Pressure
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Isolation and Identification Methods for Actinomyces israelii Involved in Actinomycosis
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作者 Osamu Tsuzukibashi Akira Fukatsu +7 位作者 Mana Fuchigami Satoshi Uchibori Chiaki Komine Koji Umezawa Sachiyo Hayashi Takashi Asano Taira Kobayashi Masahiko Fukumoto 《Open Journal of Stomatology》 2022年第4期108-118,共11页
Purpose: Actinomyces israelii is known as the key species to cause classical actinomycosis. Although A. israelii is frequently isolated from human oral cavities, the distribution of this microorganism has been little ... Purpose: Actinomyces israelii is known as the key species to cause classical actinomycosis. Although A. israelii is frequently isolated from human oral cavities, the distribution of this microorganism has been little reported. The purpose of the present study was to develop selective media (AISM) for the isolation of A. israelii and to assess the prevalence of this organism in the oral cavity. Methods: To examine the bacterial population in the oral cavity, a novel selective medium (AISM) was developed for isolating A. israelii. AISM consists of BHI, yeast extract, agar, ofloxacin, fosfomycin, colistin, and sodium fluoride. Results: A. israelii strains grew well on AISM. A. israelii was detected in all dental plaque samples collected from 20 subjects and the mean number of this organism in the samples was 7.9 × 104 CFU/ml. Conclusion: These results indicated that the selective medium was useful for the isolation of A. israelii and this organism was a part of the normal flora in the human oral cavity. 展开更多
关键词 actinomyces israelii Selective Medium Oral Cavity actinomycOSIS
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Strain and Culture Medium Optimization for Production Enhancement of Prodiginines from Marine-Derived Streptomyces sp. GQQ-10
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作者 LI Xueping ZHANG Guojian +2 位作者 ZHU Tianjiao LI Dehai GU Qianqun 《Journal of Ocean University of China》 SCIE CAS 2012年第3期361-365,共5页
A mutant (GQQ-M6) of a Sponge-Derived streptomyces sp. GQQ-10 obtained by UV-induced mutation was used for producing prodiginines (PGs). Single factor experiments and orthogonal array design (OAD) methods were employe... A mutant (GQQ-M6) of a Sponge-Derived streptomyces sp. GQQ-10 obtained by UV-induced mutation was used for producing prodiginines (PGs). Single factor experiments and orthogonal array design (OAD) methods were employed for medium optimization. In the single factor method, the effects of soluble starch, glucose, soybean flour, yeast extract and sodium acetate on PGs production were investigated individually. In the subsequent OAD experiments, the concentrations of these 5 key nutritional components combined with salinity were further adjusted. The mutant strain GQQ-M6 gave a 2.2-fold higher PGs production than that of the parent strain; OAD experiments offered a PGs yield of 61mg L-1, which was 10 times higher than that of the initial GQQ-10 strain under the original cultivation mode. 展开更多
关键词 prodiginines marine-derived streptomycete optimization culture media mutation
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Actinomycosis of the appendix mimicking appendiceal tumor:A case report 被引量:1
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作者 Sang-Yun Lee Hee-Jin Kwon +6 位作者 Jin-Han Cho Jong-Young Oh Kyung-Jin Nam Jin-Hwa Lee Seong-Kuk Yoon Myong-Jin Kang Jin-Sook Jeong 《World Journal of Gastroenterology》 SCIE CAS CSCD 2010年第3期395-397,共3页
Actinomycosis is an uncommon chronic infectious disease. Common sites of involvement include the cervicofacial, thoracic and abdominopelvic regions. In abdominopelvic actinomycosis, the ileocecal region, including the... Actinomycosis is an uncommon chronic infectious disease. Common sites of involvement include the cervicofacial, thoracic and abdominopelvic regions. In abdominopelvic actinomycosis, the ileocecal region, including the appendix, is the most commonly involved site. In some reports, limited appendiceal actinomycosis has revealed a thickened appendiceal wall with peri-appendiceal inflammation as acute appendicitis or perforated appendicitis. We experienced pathologically confirmed intraluminal limited appendiceal actinomycosis without peri-appendiceal infiltration. Here, we report the computed tomography and ultrasound findings. 展开更多
关键词 actinomyces actinomycOSIS Appendiceal neoplasms APPENDICITIS
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林可霉素产生菌:Actinomyces roseolus的变异
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作者 卢圣栋 《国际药学研究杂志》 CAS 1975年第2期124-124,共1页
本文用自然分离方法阐明林可霉素产生菌Actinomyces roseolus 981的自然变异与产量的关系。为了说明该菌株的变异性,试验了16种培养基。在黄豆饼培养基上该菌株变异性的出现率最高,故该培养基被用于变异研究。Гаузе1号培养基出现... 本文用自然分离方法阐明林可霉素产生菌Actinomyces roseolus 981的自然变异与产量的关系。为了说明该菌株的变异性,试验了16种培养基。在黄豆饼培养基上该菌株变异性的出现率最高,故该培养基被用于变异研究。Гаузе1号培养基出现变异最小,因而用于菌种保藏。 展开更多
关键词 菌落直径 actinomyces roseolus 原放线菌 林可霉素产生菌 变异性
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Esophagitis Due to Actinomycosis in an Immunocompetent Patient with a History of SARS-Cov-2 Infection: Case Report
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作者 Zamora Harvey Hernández Gustavo González Melina 《Open Journal of Gastroenterology》 2023年第10期313-318,共6页
We present an unusual case of esophageal actinomycosis that developed in a patient with normal immunity and a history of acute SARS-CoV-2 infection. We report a case of a 56-year-old female patient without chronic deg... We present an unusual case of esophageal actinomycosis that developed in a patient with normal immunity and a history of acute SARS-CoV-2 infection. We report a case of a 56-year-old female patient without chronic degenerative pathologies with a history of non-severe acute SARS-CoV-2 infection that occurred two months prior to her presentation, treated with prednisone and betamethasone orally and inhaled, respectively for 21 days. The pivotal symptom is dysphagia and definitive diagnosis requires a tissue culture of the affected organ or a biopsy that shows the classic sulfur granules. Currently, antibiotic treatment with beta-lactams, such as amoxicillin/clavulanic acid, is still effective, as it was in our patient. 展开更多
关键词 actinomyces actinomycOSIS ESOPHAGITIS SARS-CoV-2 IMMUNOCOMPETENT
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Study on Marine actinomycetes and analysis of their secondary metabolites
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作者 Bao-Liang Xu Yuan-Yuan Wang Chun-Ming Dong 《Life Research》 2023年第4期1-15,共15页
Actinomycetes are relatively prevalent bacteria in the ocean,constituting 9% of the total number of marine bacteria.The advancement of science and technology has led to a more profound exploration of marine actinomyce... Actinomycetes are relatively prevalent bacteria in the ocean,constituting 9% of the total number of marine bacteria.The advancement of science and technology has led to a more profound exploration of marine actinomycetes.These studies hold immense significance in comprehending the distribution and adaptation of marine actinomycetes within the oceanic environment,as well as uncovering new secondary metabolites.Based on differing lifestyles,marine actinomycetes can be categorized as free-living or co-epiphytic.The activity and metabolism of actinomycetes vary across diverse marine settings,including the deep sea,benthic regions,and marine organisms.Due to their distinctive biological traits and genetic background,these marine actinomycetes inevitably generate metabolites possessing unique structures.Research methodologies concerning marine actinomycetes predominantly encompass traditional pure culture techniques,molecular biology approaches,and the integration of metagenomics and bioinformatics.The exploration of varied methodologies proves pivotal for the analysis of metabolite processes.Through the cultivation of marine actinomycetes,numerous compounds featuring novel structures and significant activities have been isolated,furnishing a substantial foundation for new drug investigations.These encompass,but are not restricted to,peptides,antibiotics,terpenoids,ketones,quinones,macrolides,and pigments.The potential applications of marine actinomyces and their secondary metabolites extend beyond antibacterial and anti-tumor effects,exhibiting promising prospects in antifungal and antiviral domains.This paper provides a comprehensive review of the classification,resources,research methodologies,and habitats of marine actinomycetes.Furthermore,it delves into the classification of secondary metabolites and their functional activities,facilitating a more exhaustive analysis of the secondary metabolites produced by marine actinomycetes. 展开更多
关键词 Marine actinomyces secondary metabolites research method functional activity
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放线菌LA-5主要抑菌成分及对灰葡萄孢的抑制作用 被引量:1
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作者 陈丹丹 李培谦 +2 位作者 赵燕飞 冯宝珍 丁春爽 《核农学报》 CAS CSCD 北大核心 2024年第1期101-109,共9页
灰葡萄孢引起的灰霉病是葡萄采后重要病害之一。为筛选拮抗葡萄灰霉病的生防菌并明确其主要抑菌成分,本研究采用生长速率法分析了拮抗链霉菌LA-5发酵提取物的抑菌活性,测定了菌株LA-5发酵液提取物拮抗活性和稳定性,利用气相色谱-质谱联... 灰葡萄孢引起的灰霉病是葡萄采后重要病害之一。为筛选拮抗葡萄灰霉病的生防菌并明确其主要抑菌成分,本研究采用生长速率法分析了拮抗链霉菌LA-5发酵提取物的抑菌活性,测定了菌株LA-5发酵液提取物拮抗活性和稳定性,利用气相色谱-质谱联用技术(GC-MS)分析了代谢产物的主要成分。结果表明,LA-5发酵提取物对灰葡萄孢具有明显的抑制作用,其对灰葡萄孢的最低抑菌浓度(MIC)和最低杀菌浓度(MFC)分别为9和14 mg·L^(-1)。LA-5发酵液提取物耐高温、酸和紫外照射,对病原菌具有较稳定的拮抗活性。GC-MS分析结果显示,LA-5发酵液中的主要活性成分有17种,其中邻苯二甲酸二丁酯(DBP)含量高达52.87%。DBP对灰葡萄孢菌丝生长及分生孢子萌发均具有强烈的抑制作用。本研究为灰霉病的生物防治提供了理论基础。 展开更多
关键词 放线菌 抑制 气相色谱-质谱联用法 灰葡萄孢 生物防治
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放线菌4-1的鉴定及其发酵液抑菌作用和稳定性探究
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作者 郭宇豪 刘绍波 +1 位作者 葛菁萍 平文祥 《中国农学通报》 2024年第9期117-123,共7页
旨在挖掘盐碱地菌种资源并对其抑菌功能进行检测。以自行分离的放线菌4-1为候选菌株,利用形态学、生理生化以及分子生物学手段对其种属地位进行综合鉴定;采用琼脂扩散法和菌丝生长速率抑制法对其发酵液抑菌活性和稳定性进行评价。结果表... 旨在挖掘盐碱地菌种资源并对其抑菌功能进行检测。以自行分离的放线菌4-1为候选菌株,利用形态学、生理生化以及分子生物学手段对其种属地位进行综合鉴定;采用琼脂扩散法和菌丝生长速率抑制法对其发酵液抑菌活性和稳定性进行评价。结果表明,菌株4-1与紫赤链霉菌(Streptomyces puniceus)最为相似,同源性高达99.93%,建立的系统发育树中二者亲缘关系最为相近;菌株4-1发酵液的抑菌谱比较广泛,对病原性细菌(6株)和病原性真菌(5株)都具有一定的抑制效果;酸碱、热、紫外线对发酵液稳定性影响较小,但当温度高于90℃或pH<4时,发酵液抑菌活性下降。该菌种资源的获得及功能评价,为进一步扩大根际促生细菌种类及应用奠定了菌种基础。 展开更多
关键词 放线菌 紫赤链霉菌 菌株鉴定 抑菌作用 稳定性
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食管放线菌感染1例
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作者 羊东杰 李文宇 李陈婕 《世界华人消化杂志》 CAS 2024年第7期534-537,共4页
背景食管放线菌病是一种罕见的食管感染性疾病,常见症状包括吞咽困难和吞咽痛,这些症状通常是非特异性的,易导致误诊或延迟诊断.病例简介85岁女性,有9年的吞咽困难症状,加重9 d.她合并了高血压病、慢性阻塞性肺疾病及脑梗塞病史.胃镜发... 背景食管放线菌病是一种罕见的食管感染性疾病,常见症状包括吞咽困难和吞咽痛,这些症状通常是非特异性的,易导致误诊或延迟诊断.病例简介85岁女性,有9年的吞咽困难症状,加重9 d.她合并了高血压病、慢性阻塞性肺疾病及脑梗塞病史.胃镜发现食管多发溃疡并狭窄,后续组织病理报告确诊为食管放线菌病.抗菌治疗临床缓慢改善.结论食管放线菌病临床表现非特异性,组织病理学是目前临床确诊放线菌感染的主要检查方法.高效抗生素长期治疗,本病预后良好. 展开更多
关键词 放线菌 放线菌病 食管 病例报告
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药用昆虫桂花蝉共生放线菌的分离培养及抗菌活性研究
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作者 苏雅琳 廖婧阳 +1 位作者 欧健鹏 刘文彬 《广东药科大学学报》 CAS 2024年第2期7-15,共9页
目的探讨药用昆虫桂花蝉肠道共生放线菌的多样性,为抗菌药物开发提供新的微生物资源。方法采用平板稀释法对桂花蝉共生放线菌进行分离和纯化;采用牛津杯法测定菌株抗菌活性;通过外观观察、扫描电镜和16S rDNA序列分析进行分类鉴定;对具... 目的探讨药用昆虫桂花蝉肠道共生放线菌的多样性,为抗菌药物开发提供新的微生物资源。方法采用平板稀释法对桂花蝉共生放线菌进行分离和纯化;采用牛津杯法测定菌株抗菌活性;通过外观观察、扫描电镜和16S rDNA序列分析进行分类鉴定;对具有良好抗菌活性的菌株进行Nanopore第3代测序;利用Antismash、Prism、Circos、Rodigal、eggNOG、GO和KEGG等工具对基因组数据进行注释分析。结果采用6种培养基从桂花蝉肠道共分离获得34株不同放线菌,其中链霉菌属(Streptomyces)32株、戈登氏菌属(Gordonia)1株和冢村氏菌属(Tsukamurella)1株,菌株GHC 11可能为1株新的链霉菌种,73.5%的菌株对至少1种病原菌具有拮抗作用。菌株GHC 54具有显著的抗S.aureus和MRSA活性,通过形态学和分子生物学方法鉴定为Gordonia terrae。菌株GHC 54基因组大小为5346952 bp,包含1个5210251 bp环状染色体和1个136601 bp质粒,共编码4828个基因,GC含量为67.91%,其中含有50个tRNA基因、9个rRNA基因。编码基因分别进行eggNOG、GO、KEGG、Nr、Pfam、Swiss-prot和TrEMBL功能注释,共注释到4776个蛋白。菌株GHC 54中含有14个生物合成基因簇,包含6个NRPS、2个Terpene、1个Ectoine、1个Arylpolyene、1个Redox-cofactor、1个NAPAA、1个Ripp-like和1个PKS基因簇,85.7%的基因簇同源性小于20%,具有较高的新颖性。结论桂花蝉共生放线菌具有良好的抗菌活性,菌株GHC 54具有合成新颖抗菌化合物的潜力。本研究将为抗菌药物的开发提供新的微生物资源。 展开更多
关键词 桂花蝉 共生放线菌 戈登氏菌 全基因组测序
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滨海盐土放线菌的分离、鉴定和抗菌活性研究
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作者 施宣任 刘汉宇 王普 《发酵科技通讯》 CAS 2024年第3期174-179,共6页
从采集的滨海盐土样品中分离出多株放线菌,通过滤纸片法从中筛选具有抗菌活性的菌株,对活性菌株进行鉴定和发酵条件优化。实验筛选得到可产生不同抗菌活性物质的3株链霉菌,其中菌株ZJPH2021031的发酵液对金黄色葡萄球菌呈现出较质量浓度... 从采集的滨海盐土样品中分离出多株放线菌,通过滤纸片法从中筛选具有抗菌活性的菌株,对活性菌株进行鉴定和发酵条件优化。实验筛选得到可产生不同抗菌活性物质的3株链霉菌,其中菌株ZJPH2021031的发酵液对金黄色葡萄球菌呈现出较质量浓度为50 mmol/L的硫酸链霉素更高的抑制活性;菌株ZJPH2021032的发酵液对革兰氏阳性菌和真菌的生长具有抑制作用;菌株ZJPH2021033的发酵液则仅对真菌表现出抑制活性。采用含多种金属离子和以氨基酸为氮源的培养基发酵时,菌株ZJPH2021031的发酵液对金黄色葡萄球菌的抑制活性得到显著提升,抑菌圈直径增大了2.16倍,且培养基中的甘油质量浓度对抗菌活性物质产生显著的影响。 展开更多
关键词 菌种筛选 放线菌 抗菌剂 发酵 16S rDNA
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双样本孟德尔随机化分析肠道菌群与肝硬化的因果关系
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作者 周展毅 楼毅杰 +3 位作者 纪云西 洪耀南 王玉杭 李海涛 《安徽医药》 CAS 2024年第11期2168-2172,共5页
目的使用双样本孟德尔随机化(MR)分析肠道菌群与肝硬化的因果关系,阐明个体基因组特征对精准干预病人肠道菌群以改善肝硬化的可能性。方法该研究于2023年9―12月获取肠道微生物群等位基因单核苷酸多态性(SNP)(13266例样本)的工具变量及... 目的使用双样本孟德尔随机化(MR)分析肠道菌群与肝硬化的因果关系,阐明个体基因组特征对精准干预病人肠道菌群以改善肝硬化的可能性。方法该研究于2023年9―12月获取肠道微生物群等位基因单核苷酸多态性(SNP)(13266例样本)的工具变量及肝硬化相关等位基因SNP(1011例欧洲病例和356077例欧洲对照样本)的结局数据,使用MR分析肠道菌群对肝硬化的因果关系。结果随机效应下的逆方差加权(IVW)分析结果表明,放线菌属[P=0.001,OR=1.39,95%CI:(1.13,1.71)],普雷沃菌属[P=0.017,OR=1.27,95%CI:(1.04,1.54)]与肝硬化呈正相关。甲烷短杆菌属[P=0.024,OR=0.86,95%CI:(0.75,0.98)],乳酸菌属[P=0.042,OR=0.82,95%CI:(0.68,0.99)]与肝硬化呈负相关。结论放线菌属、普雷沃菌属与肝硬化存在因果关系,具有正面促进效应。 展开更多
关键词 肝硬化 肠道微生物群 孟德尔随机化分析 放线菌属 普雷沃菌属
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原发性泪小管炎119例诊治体会及病原学特征分析
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作者 王庆华 张正威 +4 位作者 王秋红 陆水 顾晓波 郭亮 蒋韵佳 《国际眼科杂志》 2024年第1期144-148,共5页
目的:探讨原发性泪小管炎的临床特征、诊疗经验以及病原微生物的分布特征,为原发性泪小管炎的诊治提供一定的参考。方法:回顾性临床研究。纳入2019-06/2023-02期间在无锡市第二人民医院眼科诊断为原发性泪小管炎119例120眼。治疗方法主... 目的:探讨原发性泪小管炎的临床特征、诊疗经验以及病原微生物的分布特征,为原发性泪小管炎的诊治提供一定的参考。方法:回顾性临床研究。纳入2019-06/2023-02期间在无锡市第二人民医院眼科诊断为原发性泪小管炎119例120眼。治疗方法主要分为保守治疗(经泪点清除泪小管结石联合管内注入抗生素眼膏)和手术治疗。病原微生物检查方法包括分泌物涂片镜检和微生物培养。结果:原发性泪小管炎多见于中老年女性,主要表现为长期眼红、分泌物增多,但多不伴有流泪症状。118例(99.2%)单眼发病,下泪小管发病63例63眼(52.5%)。实验室检查:119例120眼中有4例4眼未行实验室检查,余115例116眼检查结果如下:分泌物涂片革兰染色镜检,102例103眼(88.8%)检出放线菌,真菌涂片均为阴性;微生物培养,85例86眼(74.1%)细菌培养阳性。共培养出111株细菌,26种菌株。其中需氧菌32株(28.8%),厌氧菌26株(23.4%),兼性厌氧菌53株(47.7%)。最常见菌种为:链球菌20株,葡萄球菌13株,丙酸杆菌和二氧化碳嗜纤维菌各10株。放线菌仅4例4眼(3.4%)培养阳性,真菌培养均为阴性。治疗情况:119例120眼中,114例115眼(95.8%)通过保守治疗经泪点清除泪小管结石联合管内注入抗生素眼膏(微创治疗方法,IOI)后痊愈,5例5眼保守治疗效果不佳,接受手术治疗后均达到治愈,总的治愈率100.0%。结论:原发泪小管炎发病率低,好发于中老年女性,单眼下泪小管多见,临床易漏诊误诊,放线菌为主要致病菌,多为混合感染,菌种异质,厌氧或兼性厌氧菌为主,链球菌、葡萄球菌最为常见。真菌性泪小管炎罕见。泪小管炎确诊后治愈率高,推荐IOI法作为泪小管炎初始治疗。 展开更多
关键词 泪小管炎 病原学 放线菌 细菌 诊治
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美洲大蠊粪便中微生物的分离和鉴定及其抗菌活性分析
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作者 官婷婷 许文慧 +2 位作者 肖光帅 杨大松 杨银河 《昆虫学报》 CAS CSCD 北大核心 2024年第9期1190-1199,共10页
【目的】探究美洲大蠊Periplaneta americana粪便中具有抗菌活性的真菌和放线菌,为抗菌药物的开发提供微生物来源。【方法】利用稀释涂布法与选择培养法,从云南省大理白族自治州巍山县美洲大蠊养殖场收集的美洲大蠊成虫粪便中分离纯化... 【目的】探究美洲大蠊Periplaneta americana粪便中具有抗菌活性的真菌和放线菌,为抗菌药物的开发提供微生物来源。【方法】利用稀释涂布法与选择培养法,从云南省大理白族自治州巍山县美洲大蠊养殖场收集的美洲大蠊成虫粪便中分离纯化放线菌和真菌;采用牛津杯琼脂扩散法检测菌株代谢产物对6种病原细菌大肠杆菌Escherichia coli、粪肠球菌Enterococcus faecalis、金黄色葡萄球菌Staphylococcus aureus、耐甲氧西林的金黄色葡萄球菌(methicillin-resistant S.aureus)、铜绿假单胞菌Pseudomonas aeruginosa和鼠伤寒沙门氏菌Salmonella typhimurium的抗菌活性;通过16S rDNA和ITS rDNA基因测序鉴定抗菌活性菌株,并利用邻接法构建抗菌活性菌株系统发育树。【结果】从美洲大蠊成虫粪便中分离出真菌17株,放线菌19株。共有6株真菌和12株放线菌表现出不同程度的抗菌活性,其中真菌EPAF15-EPAF17及放线菌EPAA12和EPAA17对大肠杆菌、金黄色葡萄球菌、耐甲氧西林的金黄色葡萄球菌、铜绿假单胞菌和鼠伤寒沙门氏菌表现出较为广谱的抗菌活性。系统发育分析显示,具有抗菌活性的真菌隶属于镰刀菌属Fusarium、生丝毕赤酵母属Hyphopichia和丝孢酵母属Trichosporon;具有抗菌活性的放线菌隶属于链霉属Streptomyces。【结论】美洲大蠊成虫粪便中具有种类丰富的可培养微生物,其中的抗菌活性真菌和放线菌的发现不仅能作为进一步深入挖掘新型抗菌活性天然药物的重要微生物储备资源,也为特境微生物资源的充分利用提供了新的思路。 展开更多
关键词 美洲大蠊 粪便 真菌 放线菌 抗菌活性 分子鉴定 系统发育
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