The paper mainly talks about the background of S. T. Coleridge and the images of The Rime of the AncientMariner. As for the images, the wind, the albatross and the snake will be thoroughly discussed. There are manydif...The paper mainly talks about the background of S. T. Coleridge and the images of The Rime of the AncientMariner. As for the images, the wind, the albatross and the snake will be thoroughly discussed. There are manydifferent images which also have different meanings and connotations in the context. What＇s more, the poemexpresses the theme of the metabolic relationships between men and nature： harmony, conflict or compromise.展开更多
The narrator of Melvihe’s tale says of Bartleby that he is“one of those beingsof whom nothing is ascertainable.”In other words,Bartleby is a mysterious figureto the narrator.What the narrator has do,is make sense o...The narrator of Melvihe’s tale says of Bartleby that he is“one of those beingsof whom nothing is ascertainable.”In other words,Bartleby is a mysterious figureto the narrator.What the narrator has do,is make sense of Bartleby.展开更多
Generally, Coleridge’s poems are mainly divided into two groups:one is to use common Language to depict commom things and common people; the other is to use unique imagination to describe supernatural things. "T...Generally, Coleridge’s poems are mainly divided into two groups:one is to use common Language to depict commom things and common people; the other is to use unique imagination to describe supernatural things. "The Rime of the Ancient Mariner"belongs to the second group. Some critics blame Coleridge for his lack of morals in this poem, but in fact,this poem contains lots of morals, as Coleridge himself said.Coleridge succeeds in exploring the theme of sin and punishment by his rich imagination and by the depicting of supernatural things. In "The Rime of the Ancient Mariner", Coleridge intentionally built a symbolic world of the supernatural wind, the bird, the sun, the moon ,the ship and the sea. All these symbols serve to develop the theme of Fall and Redemption, and play so important a part in the poem that we even can take it as a symbolic poem. In the following pages, I am going to devote to the functions of the supernatural wind in particular. Coleridge bestows the wind with a great deal of展开更多
One of the most typical Poems in the nineteenth century is The Rime of the Ancient Mariner, written by Samue 1 Taylor Coleridge。This Poem is unique in the history of ballad writing. The eerie incidents of the story t...One of the most typical Poems in the nineteenth century is The Rime of the Ancient Mariner, written by Samue 1 Taylor Coleridge。This Poem is unique in the history of ballad writing. The eerie incidents of the story the spell of th e supernatural, and th e sustained a rchaic style,all con tribu te to make it an unforgettable Poem,PoPular alive with young and old.This article is going to deal with th ree questions:(1)the them of the Poem ( 2 ) the function of the color images and their symbolic representation ?(3) the conceP t of Life一in—Death ?展开更多
Comprehensive identification of conditionally essential genes requires efficient tools for generating high-density transposon libraries that, ideally, can be analysed using next-generation sequencing methods such as T...Comprehensive identification of conditionally essential genes requires efficient tools for generating high-density transposon libraries that, ideally, can be analysed using next-generation sequencing methods such as Transposon Directed Insertion-site Sequencing (TraDIS). The Himar1 (mariner) transposon is ideal for generating near-saturating mutant libraries, especially in AT-rich chromosomes, as the requirement for integration is a TA dinucleotide, and this transposon has been used for mutagenesis of a wide variety of bacteria. However, plasmids for mariner delivery do not necessarily work well in all bacteria. In particular, there are limited tools for functional genomic analysis of Pasteurellaceae species of major veterinary importance, such as swine and cattle pathogens, Actinobacillus pleuropneumoniae and Pasteurella multocida, respectively. Here, we developed plasmids, pTsodCPC9 and pTlacPC9 (differing only in the promoter driving expression of the transposase gene), that allow delivery of mariner into both these pathogens, but which should also be applicable to a wider range of bacteria. Using the pTlacPC9 vector, we have generated, for the first time, saturating mariner mutant libraries in both A. pleuropneumoniae and P. multocida that showed a near random distribution of insertions around the respective chromosomes as detected by TraDIS. A preliminary screen of 5000 mutants each identified 8 and 14 genes, respectively, that are required for growth under anaerobic conditions. Future high-throughput screening of the generated libraries will facilitate identification of mutants required for growth under different conditions, including in vivo, highlighting key virulence factors and pathways that can be exploited for development of novel therapeutics and vaccines.展开更多
Qingdao Ocean Shipping Mariners College is a College subordinate to China Ocean Shipping (Group) Company under the Ministry of Communications. In our college there are the Department of Marine Navigation,Department of...Qingdao Ocean Shipping Mariners College is a College subordinate to China Ocean Shipping (Group) Company under the Ministry of Communications. In our college there are the Department of Marine Navigation,Department of Marine Engineering, Department of Shipping Management and Department of Foreign Languages, offering speciahies of Marine Navigation, Marine Engine Control, Marine Electrical Equipment Control, Marine Radio Communication, International Shipping Management and International Finance and Accounting. We have 3 types of education: the education of formal college schooling, the training for implementation of the 95 revised STCW Convention and the continuation education. Meanwhile we undertake different training of COSCO’s contracted seafarers overseas and other managerial per-展开更多
A remarkable marine heatwave,known as the“Blob”,occurred in the Northeast Pacific Ocean from late 2013 to early 2016,which displayed strong warm anomalies extending from the surface to a depth of 300 m.This study em...A remarkable marine heatwave,known as the“Blob”,occurred in the Northeast Pacific Ocean from late 2013 to early 2016,which displayed strong warm anomalies extending from the surface to a depth of 300 m.This study employed two assimilation schemes based on the global Climate Forecast System of Nanjing University of Information Science(NUIST-CFS 1.0)to investigate the impact of ocean data assimilation on the seasonal prediction of this extreme marine heatwave.The sea surface temperature(SST)nudging scheme assimilates SST only,while the deterministic ensemble Kalman filter(EnKF)scheme assimilates observations from the surface to the deep ocean.The latter notably improves the forecasting skill for subsurface temperature anomalies,especially at the depth of 100-300 m(the lower layer),outperforming the SST nudging scheme.It excels in predicting both horizontal and vertical heat transport in the lower layer,contributing to improved forecasts of the lower-layer warming during the Blob.These improvements stem from the assimilation of subsurface observational data,which are important in predicting the upper-ocean conditions.The results suggest that assimilating ocean data with the EnKF scheme significantly enhances the accuracy in predicting subsurface temperature anomalies during the Blob and offers better understanding of its underlying mechanisms.展开更多
文摘The paper mainly talks about the background of S. T. Coleridge and the images of The Rime of the AncientMariner. As for the images, the wind, the albatross and the snake will be thoroughly discussed. There are manydifferent images which also have different meanings and connotations in the context. What＇s more, the poemexpresses the theme of the metabolic relationships between men and nature： harmony, conflict or compromise.
文摘The narrator of Melvihe’s tale says of Bartleby that he is“one of those beingsof whom nothing is ascertainable.”In other words,Bartleby is a mysterious figureto the narrator.What the narrator has do,is make sense of Bartleby.
文摘Generally, Coleridge’s poems are mainly divided into two groups:one is to use common Language to depict commom things and common people; the other is to use unique imagination to describe supernatural things. "The Rime of the Ancient Mariner"belongs to the second group. Some critics blame Coleridge for his lack of morals in this poem, but in fact,this poem contains lots of morals, as Coleridge himself said.Coleridge succeeds in exploring the theme of sin and punishment by his rich imagination and by the depicting of supernatural things. In "The Rime of the Ancient Mariner", Coleridge intentionally built a symbolic world of the supernatural wind, the bird, the sun, the moon ,the ship and the sea. All these symbols serve to develop the theme of Fall and Redemption, and play so important a part in the poem that we even can take it as a symbolic poem. In the following pages, I am going to devote to the functions of the supernatural wind in particular. Coleridge bestows the wind with a great deal of
文摘One of the most typical Poems in the nineteenth century is The Rime of the Ancient Mariner, written by Samue 1 Taylor Coleridge。This Poem is unique in the history of ballad writing. The eerie incidents of the story the spell of th e supernatural, and th e sustained a rchaic style,all con tribu te to make it an unforgettable Poem,PoPular alive with young and old.This article is going to deal with th ree questions:(1)the them of the Poem ( 2 ) the function of the color images and their symbolic representation ?(3) the conceP t of Life一in—Death ?
基金This work was supported by a Longer and Larger(LoLa)grant from the Biotechnology and Biological Sciences Research Council(BBSRC,grant numbers BB/G020744/1,BB/G019177/1,BB/G019274/1 and BB/G018553/1)the UK Department for Environment,Food and Rural Affairs and Zoetis awarded to the Bacterial Respiratory Diseases of Pigs-1 Technology(BRaDP1T)consortium.Funding for LZ was provided by the BBSRC(grant number BB/C508193/1)The funders had no role in study design,data collection and analysis,decision to publish,or preparation of the manuscript.
文摘Comprehensive identification of conditionally essential genes requires efficient tools for generating high-density transposon libraries that, ideally, can be analysed using next-generation sequencing methods such as Transposon Directed Insertion-site Sequencing (TraDIS). The Himar1 (mariner) transposon is ideal for generating near-saturating mutant libraries, especially in AT-rich chromosomes, as the requirement for integration is a TA dinucleotide, and this transposon has been used for mutagenesis of a wide variety of bacteria. However, plasmids for mariner delivery do not necessarily work well in all bacteria. In particular, there are limited tools for functional genomic analysis of Pasteurellaceae species of major veterinary importance, such as swine and cattle pathogens, Actinobacillus pleuropneumoniae and Pasteurella multocida, respectively. Here, we developed plasmids, pTsodCPC9 and pTlacPC9 (differing only in the promoter driving expression of the transposase gene), that allow delivery of mariner into both these pathogens, but which should also be applicable to a wider range of bacteria. Using the pTlacPC9 vector, we have generated, for the first time, saturating mariner mutant libraries in both A. pleuropneumoniae and P. multocida that showed a near random distribution of insertions around the respective chromosomes as detected by TraDIS. A preliminary screen of 5000 mutants each identified 8 and 14 genes, respectively, that are required for growth under anaerobic conditions. Future high-throughput screening of the generated libraries will facilitate identification of mutants required for growth under different conditions, including in vivo, highlighting key virulence factors and pathways that can be exploited for development of novel therapeutics and vaccines.
文摘Qingdao Ocean Shipping Mariners College is a College subordinate to China Ocean Shipping (Group) Company under the Ministry of Communications. In our college there are the Department of Marine Navigation,Department of Marine Engineering, Department of Shipping Management and Department of Foreign Languages, offering speciahies of Marine Navigation, Marine Engine Control, Marine Electrical Equipment Control, Marine Radio Communication, International Shipping Management and International Finance and Accounting. We have 3 types of education: the education of formal college schooling, the training for implementation of the 95 revised STCW Convention and the continuation education. Meanwhile we undertake different training of COSCO’s contracted seafarers overseas and other managerial per-
基金supported by the National Natural Science Foundation of China [grant number 42030605]the National Key R&D Program of China [grant number 2020YFA0608004]。
文摘A remarkable marine heatwave,known as the“Blob”,occurred in the Northeast Pacific Ocean from late 2013 to early 2016,which displayed strong warm anomalies extending from the surface to a depth of 300 m.This study employed two assimilation schemes based on the global Climate Forecast System of Nanjing University of Information Science(NUIST-CFS 1.0)to investigate the impact of ocean data assimilation on the seasonal prediction of this extreme marine heatwave.The sea surface temperature(SST)nudging scheme assimilates SST only,while the deterministic ensemble Kalman filter(EnKF)scheme assimilates observations from the surface to the deep ocean.The latter notably improves the forecasting skill for subsurface temperature anomalies,especially at the depth of 100-300 m(the lower layer),outperforming the SST nudging scheme.It excels in predicting both horizontal and vertical heat transport in the lower layer,contributing to improved forecasts of the lower-layer warming during the Blob.These improvements stem from the assimilation of subsurface observational data,which are important in predicting the upper-ocean conditions.The results suggest that assimilating ocean data with the EnKF scheme significantly enhances the accuracy in predicting subsurface temperature anomalies during the Blob and offers better understanding of its underlying mechanisms.
