CK19、HBME-1、CD56、BRAF V600E、FAM210B在甲状腺乳头状结构诊断中的应用

目的探讨CK19、HBME-1、CD56、BRAF V600E和FAM210B蛋白表达在甲状腺乳头状结构诊断中的作用及FAM210B和BRAF V600E与甲状腺乳头状癌临床病理特征的关系。方法连续收集2017年1月—2020年9月××省第二人民医院PTC患者的病理标本279例,甲状腺乳头状增生标本85例。用免疫组化SP法检测甲状腺乳头状癌(papillary thyroid carcinoma,PTC)和甲状腺乳头状增生中CK19、HBME-1、CD56、BRAF V600E和FAM210B的表达情况,分析FAM210B和BRAF V600E在不同临床病理特征的PTC组织中的表达情况。结果CD56在PTC组织中表达率为10.03%,在甲状腺乳头状增生中表达率为94.12%;CK19、HBME-1、BRAF V600E、FAM210B在PTC组织中的阳性率均高于甲状腺乳头状增生,差异有统计学意义(P<0.01);FAM210B表达与年龄、肿块大小和淋巴结转移情况具有相关性,BRAF V600E表达与性别、年龄、肿块大小和淋巴结转移情况具有相关性,差异有统计学意义(P<0.01)。结论CK19、HBME-1、FAM210B、BRAF V600E联合CD56标记有助于PTC的诊断和鉴别诊断;检测FAM210B和BRAF V600E有助于评估肿瘤的生物学行为及预后,对指导治疗和提高疗效有重要意义。

Efficacy of a Diabetes Specific Nutrition Supplement on Glycemic, Anthropometric, Dietary and Gut Health Markers in Adults with Type 2 Diabetes: An RCT

With increasing incidence of diabetes, use of diabetes specific nutrition supplements (DSNS) is common for better management of the disease. To study effect of 12-week DSNS supplementation on glycemic markers, anthropometry, lipid profile, SCFAs, and gut microbiome in individuals with diabetes. Markers studied were glycemic [Fasting Blood Glucose (FBG), Post Prandial Glucose (PPG), HbA1c, Incremental Area under curve (iAUC), Mean Amplitude of Glycemic Excursions (MAGE), Time in/above Range (TIR/TAR)], anthropometry [weight, Body Mass Index (BMI), waist circumference (WC)], lipid profile, diet and gut health [plasma short chain fatty acids (SCFAs)]. N = 210 adults were randomized to receive either DSNS with standard care (DSNS + SC;n = 105) or standard care alone (SC alone;n = 105). After 12 weeks, significant differences between DSNS + SC versus SC alone was observed in FBG [−3 ± 6 vs 14 ± 6 mg/dl;p = 0.03], PPG [−35 ± 9 vs −3 ± 9 mg/dl;p = 0.01], weight [−0.6 ± 0.1 vs 0.2 ± 0.1 kg;p = 0.0001], BMI [−0.3 ± 0.1 vs 0.1 ± 0.1 kg/m2;p = 0.0001] and WC [−0.3 ± 0.2 vs 0.2 ± 0.2 cm;p = 0.01]. HbA1C and low-density lipoprotein (LDL) were significantly reduced in DSNS + SC [−0.2 ± 0.9;p = 0.04 and −5 mg/dl;p = 0.03] respectively with no change in control. Continuous Glucose Monitoring (CGM) reported significant differences between DSNS + SC versus SC alone for mean glucose [−12 ± 65 vs 28 ± 93 mg/dl;p < 0.01], TAR 180 [−9 ± 42 vs 7 ± 45 mg/dl;p = 0.04], TAR 250 [−3 ± 27 vs 9 ± 38 mg/dl;p = 0.05], iAUC [−192 (1.1) vs −48 (1.1) mg/dl;p = 0.03]. MAGE was significantly reduced for both DSNS + SC (−19 ± 67;p < 0.001) and SC alone (−8 ± 70;p = 0.04), with reduction being more pronounced for DSNS + SC. DSNS + SC reported a decrease in carbohydrate energy % [−9.4 (−11.3, −7.6) %;p < 0.0001] and amount [−47.4 (−67.1, −27.7) g;p < 0.0001], increased dietary fiber [9.5 (7.2, 11.8) g;p < 0.0001] and protein energy % [0.9 (0.5, 1.3) %;p < 0.0001] versus SC alone. DSNS + SC reported significant increases versus SC alone in total (0.3 ng/ml;p = 0.03) and individual plasma SCFAs. The consumption of DSNS significantly improves the glycemic, anthropometric, dietary, and gut health markers in diabetes.

Development and Utilization of 1S^1 Chromosomearm-specific Molecular Markers of Aegilops longissima

Introducing the 1S^1 chromosome of Aegilops longissima into wheat genome can significantly improve wheat grain quality and contents of iron and zinc. Therefore, the development of molecular markers specific to 1S^1 chromosome of A. longissima is of important significance for breeding high-quality wheat with high contents of iron and zinc in grains. In this study, nine molecular markers specific to 1S^1 chromosome of A. longissima were developed, including two 1S^1S specific markers,six 1S^1L specific markers and one 1S^1 specific marker which was located on both short and long arms. The practicability of these molecular markers were verified using hybrid population as materials. The results showed that hybrid population could be effectively screened and identified, which indicated that the developed 1S^1 chromosome-specific molecular markers could be used for screening and identification of hybrid population and could be used in marker-assisted breeding of high-quality wheat with high contents of Fe and Zn in grains.

BRAF^(V600E)、CK19、HBME-1和CD56四种抗体联合检测在甲状腺乳头状癌诊断中的应用价值

目的检测BRAF^(V600E)、CK19、HBME-1和CD56抗体在甲状腺乳头状癌(PTC)结节和甲状腺良性结节的表达水平,探讨四种免疫标记联合使用的诊断价值。方法选取2021年11月至2022年11月昆山市第一人民医院病理科179例甲状腺手术标本,其中62例结节性甲状腺肿、腺瘤等良性结节为良性组,117例PTC结节为恶性组。采用免疫组织化学法检测BRAF^(V600E)、CK19、HBME-1和CD56抗体表达水平并分析其检测效能。结果恶性组BRAF^(V600E)、CK19、HBME-1、CD56阳性表达率分别为85.47%、100.00%、99.15%、6.84%,良性组的阳性表达率分别为4.84%、41.94%、8.06%、90.32%,差异有统计学意义(P<0.05)。BRAF^(V600E)、CK19、HBME-1、CD56诊断PTC灵敏度分别为85.47%、100.00%、99.15%、93.16%;特异度分别为95.16%、58.06%、91.93%、90.32%;准确性分别为88.83%、85.47%、96.65%、92.18%。四种抗体联合检测的灵敏度、特异度、准确性均为100.00%。四种抗体联合检测与各抗体单独检测比较,差异有统计学意义(P<0.05)。结论BRAF^(V600E)、CK19、HBME-1和CD56是临床鉴别甲状腺良性结节和PTC结节的重要指标,BRAF^(V600E)、CK19、HBME-1异常过表达和CD56表达缺失是临床诊断PTC的可靠标志物。四种抗体联合检测有效提高PTC的诊断准确性。

肿瘤内皮标记物1通过丝裂原活化蛋白激酶途径介导内皮细胞对血管新生及对心力衰竭心肌重塑

目的 基于肿瘤内皮标记物1(TEM1)介导的丝裂原活化蛋白激酶(MAPKs)途径,探讨内皮细胞对血管新生及对心力衰竭心肌重塑的作用。方法 将小鼠随机分成4组,包括假手术组、MI组、MI+sh-NC组和MI+sh-TEM1组。在心肌梗死(MI)后第7天通过免疫荧光染色检测梗死边缘区EndMT的变化,第28天通过超声心动图评估小鼠的心脏功能。小鼠主动脉内皮细胞(MAECs)分为3组:对照组、Vector组和rTEM1组。此外,用MAPK抑制剂SB203580预处理MAECs,用rTEM1处理细胞48 h。通过Western blot评估内皮细胞中EndMT和MAPKs信号通路的变化。结果 在梗死边缘区的心肌中,TEM1水平在MI后第1天轻微增加,在第7天显著达到峰值,然后在第28天降低。与Vector组相比,rTEM1组MAECs中VE-Cadherin蛋白表达显著下降(P <0.05),和α-SMA、波形蛋白蛋白水平、相对迁移距离、侵袭细胞数和形成分支数量显著增加(P <0.05)。SB203580逆转了由rTEM1诱导MAECs的这些变化。与MI组相比,MI+sh-TEM1组中的CD31+Vimentin+共染色水平显著降低(P <0.01)。在第28天,MI+sh-TEM1组小鼠的LVEF和LVFS均较MI组显著增强(P <0.05)。与MI组相比,MI+sh-TEM1组小鼠的内皮细胞中p-P38/P38和p-JNK/JNK蛋白表达降低。结论 TEM1诱导的EndMT和血管生成参与了MI诱导心肌重塑的发病机制,其作用机制与MAPKs信号通路激活有关。

血清NY-ESO-1自身抗体和MMP1联合检测对食管鳞状细胞癌的诊断价值

目的:探讨血清基质金属蛋白酶-1(matrix metalloproteinase-1,MMP1)和纽约食管鳞状细胞癌抗原-1(New York esophageal squamous cell carcinoma 1,NY-ESO-1)自身抗体联合检测在食管鳞状细胞癌中的诊断意义。方法:应用酶联免疫吸附实验检测120例食管鳞状细胞癌患者和120例正常对照血清中MMP1和NY-ESO-1自身抗体的表达水平,采用受试者工作特征(receiver operating characteristic,ROC)曲线评价诊断效能。结果:血清MMP1和NY-ESO-1自身抗体在食管鳞状细胞癌患者中的表达均明显高于正常对照[(8.070±5.738)ng/mL vs(4.331±3.137)ng/mL,Z=6.214,P<0.001;0.463±0.571 vs 0.156±0.086,Z=5.210,P<0.001]。ROC曲线显示,当血清MMP1为最佳诊断临界值10.586 ng/mL时,其在诊断食管鳞状细胞癌的曲线下面积(area under the ROC curve,AUC)为0.732(95%CI:0.671~0.787),敏感度为24.2%,特异度为95.0%。NY-ESO-1自身抗体诊断食管鳞状细胞癌AUC为0.695(95%CI:0.632~0.752),敏感度为33.0%,特异度为95.0%。MMP1和NY-ESO-1自身抗体联合检测诊断食管鳞状细胞癌的AUC为0.800(95%CI:0.744~0.849),敏感度为47.5%,特异度为95.0%。结论:血清MMP1和NY-ESO-1自身抗体联合检测可能有助于提高食管鳞状细胞癌的诊断效能。

PD-1单抗后线辅助治疗晚期结直肠癌患者对胃肠功能、肿瘤标志物的影响

目的分析程序性死亡受体-1(PD-1)单抗后线辅助治疗晚期结直肠癌患者对胃肠功能、肿瘤标志物的影响。方法前瞻性选取2020年1月至2023年1月海口市人民医院收治的105例晚期结直肠癌患者为研究对象,按照随机数字表法将患者分为对照组(n=50)、研究组(n=55)。对照组采用阿帕替尼治疗,研究组在对照组的基础上联合PD-1单抗后线治疗,3周为1个疗程,共治疗4个疗程。比较两组的临床疗效、胃肠功能改善时间,治疗前、治疗12周后的肿瘤标志物[癌胚抗原、糖类抗原(CA)72-4、CA199]、免疫功能(CD4^(+)、CD8^(+)、CD4^(+)/CD8^(+))指标以及不良反应发生情况。结果治疗12周后,研究组的客观缓解率(ORR)、病情控制率(DCR)分别为78.33%、90.91%,均高于对照组(58.00%、70.00%),差异均有统计学意义(P<0.05)。研究组患者的首次排便时间、首次排气时间、肠鸣音消退时间分别为(51.50±5.02)、(35.52±4.25)、(44.28±4.80)d,均短于对照组[(69.37±6.40)、(50.63±5.10)、(53.50±6.15)d],差异均有统计学意义(P<0.05)。治疗12周后,研究组癌胚抗原、CA72-4、CA199水平分别为(12.57±1.64)ng/mL、(17.60±3.45)U/mL、(58.29±8.70)kU/L,均低于对照组[(17.24±2.50)ng/mL、(27.59±4.67)U/mL、(82.65±12.29)kU/L],差异均有统计学意义(P<0.05)。治疗12周后,研究组CD4^(+)、CD4^(+)/CD8^(+)水平分别为(21.42±3.54)%、0.73±0.30,均低于对照组[(28.39±4.38)%、1.12±0.41],CD8^(+)水平为(30.36±4.52)%,高于对照组[(25.71±3.30)%],差异均有统计学意义(P<0.05)。研究组的不良反应发生率为9.09%,稍低于对照组(16.00%),但两组比较差异无统计学意义(P>0.05)。结论通过PD-1单抗后线辅助治疗可有效改善晚期结直肠癌患者的临床疗效,效果明显,改善胃肠功能,降低肿瘤标志物指标水平,调节机体免疫功能,可为临床干预此病提供参考。

Plasma matrix metalloproteinase-1 and tissue inhibitor of metalloproteinase-1 as biomarkers of ulcerative colitis activity

AIM:Overexpression of mucosal metalloproteinases(MMP) has been demonstrated recently in inflammatory bowel disease.Their activity can be counterbalanced by the tissue inhibitor of metalloproteinases(TIMP).The aim of this study was to evaluate the effect of ulcerative colitis(UC)on MMP- 1 and TIMP-1 plasma concentrations,as two possible biomarkers of the disease activity. METHODS:MMP-1 and TIMP-1 plasma concentrations were measured with an enzyme immunoassay in 16 patients with endoscopically confirmed active UC. RESULTS:Plasma concentrations of both MMP-1(13.7±0.2 ng/ml)and TIMP-1(799±140 ng/ml)were significantly elevated in UC patients in comparison to healthy controls (11.9±0.9 ng/ml and 220±7 ng/ml respectively).There was no correlation between TIMP-1 and MMP-1 concentrations (r=0.02).TIMP-1 levels revealed significant positive correlations with scored endoscopic degree of mucosal injury, disease activity index and clinical activity index values as well as C-reactive protein concentration.There was no correlation between MMP-1 and laboratory,clinical or endoscopic indices of the disease activity.CONCLUSION: These results confirm the role of both MMP- 1 and TIMP-1 in the pathogenesis of ulcerative colitis. However only TIMP-1 can be useful as a biomarker of the disease activity, demonstrating association with clinical and endoscopic pictures.

Host markers and correlated mutations in the overlapping genes of influenza viruses: M1, M2;NS1, NS2;and PB1, PB1-F2

The influenza A viruses have three gene segments, M, NS, and PB1, which code for more than one protein. The overlapping genes from the same segment entail their interdependence, which could be reflected in the evolutionary constraints, host distinction, and co-mutations of influenza. Most previous studies of overlapping genes focused on their unique evolutionary constraints, and very little was achieved to assess the potential impact of the overlap on other biological aspects of influenza. In this study, our aim was to explore the mutual dependence in host differentiation and co-mutations in M, NS, and PB1 of avian, human, 2009 H1N1, and swine viruses, with Random Forests, information entropy, and mutual information. The host markers and highly co-mutated individual sites and site pairs (P values < 0.035) in the three gene segments were identified with their relative significance between the overlapping genes calculated. Further, Random Forests predicted that among the three stop codons in the current PB1-F2 gene of 2009 H1N1, the significance of a mutation at these sites for host differentiation was, in order from most to least, that at 12, 58, and 88, i.e., the closer to the start of the gene the more important the mutation was. Finally, our sequence analysis surprisingly revealed that the full-length PB1-F2, if the three stop codons were all mutated, would function more as a swine protein than a human protein, although the PB1 of 2009 H1N1 was derived from human H3N2.

The Risk of Islet Cell and Insulin Autoantibodies and Their Predictive Strength as Markers of Type-1 Diabetes in a Cross-Section of Nigerian Population

Aim: We demonstrated the risk of developing islet autoantibodies-Insulin Autoanti-bodies (IAAs) and Islets cell Autoantibodies (ICAs)-in type-1 diabetic relatives and newly diagnosed type-1 patients compared to non-diabetic controls. We also aimed to determine the predictive strengths of both autoantibodies in the development of type-1 diabetes mellitus, and which of the two autoantibodies is a better predictive marker of type-1 diabetes mellitus among Nigerian adults. Methodology: A total number of four hundred and fifty five (455) subjects (211 (46%) males, and 244 (54%) females) aged between 35 - 76 years were recruited for the study. IAA and ICA levels were estimated using ELISA reagents from Biomerica Inc. Other parameters such as fasting blood sugar, urine glucose, and urine protein were assessed using standard biochemical techniques. Results: Relatives of type-1 diabetic patients and newly diagnosed type-1 diabetic patients were at greater risk (p < 0.05) of testing positive for more than one autoantibody (ICA and IAA) compared to non-diabetic controls. In addition, IAAs appeared to be better predictors or markers of type-1 diabetes mellitus compared to ICAs. Conclusion: The present study indicated a greater risk of autoim-mune destruction of the insulin producing beta cells of the pancrease of the type-1 relatives and newly diagnosed type-1 patients and suggests the need for periodic re-cruitment of individuals in the general population, siblings and relatives of type-1 diabetic patients for planned intervention trials. In addition, IAAs appeared to be better autoimmune markers of type-1 diabetes compared to ICAs.

Monocyte chemotactic protein-1 and soluble adhesion molecules as possible prognostic markers of the efficacy of antiviral treatment in chronic hepatitis C

AIM:To explain the role of Monocyte chemotactic protein-1 (MCP-1) and soluble adhesion molecules in chronic hepatitis C during the treatment of interferon alpha (IFNα) 2 b and ribavirin (RBV). METHODS:Concentrations of MCP-1,soluble adhesion molecules intercellular adhesion molecule-1 (sICAM-1),sP- selectin,interleukin (IL) 6,and IL10 in serum were estimated in the group of 40 patients with chronic hepatitis C treated with IFNalpha2 b and RBV in 0,16,32,48 wk of the therapy, RESULTS:In chronic hepatitis C,before and during the treatment,the serum levels of MCP-1 and sP-selectin in responders were similar to those of healthy subjects.In non- responders (NR),MCP-1 increased in the course of IFNc^+RBV treatment,differences were statistically significant as compared to responders.MCP-1 correlated statistically with the activity of periportal inflammation (r=0.35,P<0.05) but not with staging of liver fibrosis,sICAM-1 positively correlated with inflammatory activity and fibrosis in NR.sP-selectin did not correlate with histological findings in the liver.The MCP-1 correlated with the soluble form of sP-selectin concentrations (r= 6,P<0.001) and with IL-10 level in NR (r=0.4,P<0.05).There was no correlation observed between the concentration of MCP-1 and sICAM-1,IL-6 during the treatment. CONCLUSION:MCP-1 concentration may be a prognostic marker of the efficacy of IFN+RBV therapy in patients with chronic hepatitis C.

过敏性鼻炎患儿血清HLA-G、Claudin-1水平变化及与病情严重程度的关系

目的 探讨过敏性鼻炎患儿血清人类白细胞抗原G(HLA-G)、封闭蛋白1(Claudin-1)水平的变化及其与病情严重程度的关系。方法 选取该院2021年7月1日至2022年7月1日收治的86例过敏性鼻炎患儿作为观察组,同期在该院体检的34例健康儿童作为对照组。采用酶联免疫吸附试验检测两组血清HLA-G、Claudin-1水平并进行比较。比较治疗前及治疗过程中观察组患儿过敏性鼻炎症状量表(SFAR)和伴随症状的视觉模拟量表(VAS)评分,分析不同SFAR、VAS评分患儿血清HLA-G、Claudin-1水平。采用Spearman法分析血清HLA-G、Claudin-1水平与SFAR和VAS评分的相关性。结果 与对照组相比,观察组血清HLA-G水平较高,Claudin-1水平较低,差异均有统计学意义(P<0.05);观察组在治疗30周、1年后HLA-G水平明显降低,Claudin-1水平明显升高,差异均有统计学意义(P<0.05)。与治疗前比较,治疗30周、1年后观察组SFAR评分降低,VAS评分轻度/中重度比例减少,差异有统计学意义(P<0.05)。相关性分析结果显示,过敏性鼻炎患儿血清HLA-G水平与病情严重程度呈正相关(r_(SFAR)=0.433,P<0.05;r_(VAS)=0.442,P<0.05),Claudin-1水平与病情严重程度呈负相关(r_(SFAR)=-0.483,P<0.05;r_(VAS)=-0.468,P<0.05)。结论 过敏性鼻炎患儿血清HLA-G水平较高,Claudin-1水平较低,且与病情严重程度相关,二者具有作为诊断和评价疾病标志物的潜力。

仑伐替尼及PD-1抑制剂联合经导管动脉栓塞化疗治疗不可切除肝细胞癌临床观察

目的 探讨仑伐替尼、程序性死亡受体-1(PD-1)抑制剂联合经导管动脉栓塞化疗(TACE)治疗不可切除肝细胞癌(uHCC)的临床疗效。方法 选取安徽省阜阳市阜南县人民医院2020年7月至2023年7月收治的uHCC患者106例,按治疗方案的不同分为对照组(32例)、药物组(34例)和联合组(40例),分别予TACE、TACE+PD-1抑制剂、TACE+PD-1抑制剂+仑伐替尼方案治疗,其中PD-1抑制剂为信迪利单抗注射液、替雷利珠单抗注射液、注射用卡瑞利珠单抗中三选一。3组治疗周期均为6周。结果 联合组疾病控制率为90.00%,显著高于对照组的65.63%和药物组的70.59%(P <0.05)。联合组患者治疗后的丙氨酸氨基转移酶、天门冬氨酸氨基转移酶、总胆红素、甲胎蛋白、癌胚抗原、α-L-岩藻糖苷酶、糖类抗原199水平及生活质量核心量表评分均显著低于对照组及药物组(P <0.05)。3组不良反应发生率无显著差异(P> 0.05)。结论 仑伐替尼、PD-1抑制剂联合TACE治疗uHCC,可有效改善患者的肝功能,降低血清肿瘤标志物水平,提高患者预后的生活质量。

超声内镜、巨噬细胞抑制因子-1、糖类抗原19-9联合诊断胰腺癌的临床价值分析

目的探究超声内镜、巨噬细胞抑制因子-1(MIC-1)、血清肿瘤标志物糖类抗原19-9(CA19-9)联合诊断胰腺癌的临床价值。方法选取2019年3月至2022年3月在南京医科大学附属苏州市立医院行超声内镜检查的100例高度疑似胰腺癌患者作为观察组,同期来院进行体检的50例健康志愿者作为对照组。采用化学发光法检测CA19-9水平,采用酶联免疫法检测MIC-1水平并进行组间比较。以术后患者的病理检查结果为金标准,比较超声内镜、MIC-1、CA19-9分别及联合应用对胰腺癌的诊断价值。结果2组研究对象的血清CA19-9、MIC-1水平比较差异有统计学意义(P<0.05)。与健康对照组相比较,观察组患者的血清CA19-9、MIC-1水平更高,差异有统计学意义(P<0.05)。超声内镜、MIC-1、CA19-9三者联合诊断的敏感度(84.21%)均低于三者分别诊断(92.11%、90.79%、90.79%),特异度(91.67%)均高于三者分别诊断(79.17%、83.33%、75.00%)。三者联合诊断的阳性预测值为96.97%,阴性预测值为64.71%。结论在对胰腺肿瘤进行良恶性诊断时,采用超声内镜、MIC-1、CA19-9联合诊断可以有效提高其诊断效能。

SDC1 acts as a novel molecular marker and prevents rheumatoid arthritis and modulates inflammatory response by targeting the miR-4531/SDC1 axis

Objective:Rheumatoid arthritis(RA)is a systemic autoimmune disease characterized by chronic erosive arthritis.Due to the lack of effective biomarkers for diagnosis and treatment,RA patients have many complications in the later stage,seriously affecting their quality of life.Thus,this study was conducted to investigate new therapeutic targets and to discover diagnostic biomarkers in RA.Methods:In this study,the expression profiles of GSE55235 and GSE55457 were downloaded from the Gene Expression Omnibus database to obtain DEGs between RA and healthy samples.Genetic Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were performed on the common genes existing in the RA-related modules.Additionally,we used the STRING database to construct the protein‒protein interaction network.Furthermore,we established the interaction analysis of Hub Genes and microRNA(miRNA)and verified the 10 Hub genes through the GSE77298 dataset and quantitative real-time polymerase chain reaction Results:276 and 69 DEGs were screened from the GSE55235 dataset and GSE55457 dataset,respectively.Then,we obtained 42 up-regulated genes in two chip datasets intersection.Genetic Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis of the 42 up-regulated genes showed that they were mainly concentrated in immune response-activating cell surface receptor signaling pathway,etc.Furthermore,the protein-protein interaction network indicated that 10 hub genes are closely related to RA,including MS4A1,CD27,LCK,CD79A,SDC1,CXCL9,CXCL10,CXCL13,IGLL5,and IGJ.In addition,we found that miR-4531 is the same target miRNAs between MS4A1 and SDC1 through messenger RNA-miRNA co-expression network.Finally,the GSE77298 gene chip and quantitative real-time polymerase chain reaction verified the expression of 10 Hub genes.The six Hub genes of CD27,SDC1,CXCL9,CXCL10,CXCL13,and IGJ are significantly increased.Conclusions:We found that SDC1 may be a novel molecular marker for the prevention and treatment of RA.The miR-4531/SDC1 regulatory axis may play a key role in this process.In conclusion,our study not only provides potential biomarkers for the diagnosis and treatment of RA,but also provides a basis and new targets for further revealing the potential mechanism of RA occurrence and development and discovering targeted drugs.

桃红四物汤联合西药治疗心力衰竭合并心绞痛的效果及对ET-1、NT-proBNP及心衰标志物的影响

目的 探讨桃红四物汤联合西药治疗心力衰竭合并心绞痛的效果及对内皮素-1(ET-1)、N末端B型利钠肽原(NT-proBNP)及心衰标志物的影响。方法 选择2020年1月—2022年1月在医院接受治疗的77例心力衰竭合并心绞痛患者,采用随机数表法分为试验组39例和对照组38例。对照组给瑞舒伐他汀钙治疗,试验组给予桃红四物汤联合瑞舒伐他汀钙治疗。比较两组临床疗效、ET-1、NT-proBNP、B型利钠肽(BNP)、超敏C反应蛋白(hs-CRP)、白细胞介素6(IL-6)、发作频率、持续时间及并发症发生情况。结果 治疗后,两组总有效率比较差异显著(P<0.05);治疗后,两组血清ET-1、NT-proBNP水平均降低,试验组降低更为明显,(P<0.05);治疗后,两组血清BNP、IL-6及hs-CRP水平均降低,试验组降低更为明显,(P<0.05);治疗后,两组发作频率、持续时间均降低,试验组降低更为明显,(P<0.05);两组并发症主要为恶心呕吐、头晕及腹泻,比较无显著差异(P>0.05)。结论 在心力衰竭合并心绞痛中桃红四物汤联合瑞舒伐他汀钙具有较好的效果,可能与其可改善ET-1、NT-proBNP及心衰标志物有关。

针刺联合穴位贴敷治疗中晚期食管癌疗效观察及对楔状组织中TGF-β_(1)/Smad信号通路的影响

目的:观察针刺联合穴位贴敷治疗中晚期食管癌的临床疗效及对楔状组织中转化生长因子β_(1)(TGF-β_(1))/胰腺癌缺失因子(Smad)信号通路的影响。方法:将90例中晚期食管癌患者随机分为对照组与治疗组各45例。对照组给予常规放化疗治疗,治疗组在对照组基础上给予针刺联合穴位贴敷。观察2组治疗后临床疗效,不良反应及随访3年生存率;以及治疗前后简体中文版生活质量评估表(QLQ-C30)、SF-36健康调查量表[躯体功能(PCS)、社会功能(PSS)和情绪角色(MCS)]评分,血清肿瘤标志物[糖类抗原19-9(CA19-9)、癌胚抗原(CEA)、细胞角蛋白19片段抗原21-1(CYFRA21-1)、糖类抗原125(CA125)]、血清胃肠激素[血管活性肠(VIP)、生长抑素(SOM)、胃动素(MTL)、5-羟色胺(5-HT)]、楔状组织上清液中TGF-β_(1)/Smad信号通路(TGF-β_(1)RⅠ型受体、TGF-β_(1)RⅡ型受体、Smad4、Smad7)水平。结果:治疗后,治疗组疾病控制率为95.24%,对照组为73.17%,2组比较,差异有统计学意义(P<0.05)。治疗1、2个疗程,2组PCS、PSS、MCS评分均较治疗前升高(P<0.05),且治疗组相同时间点PCS、PSS、MCS评分均高于对照组(P<0.05)。治疗1、2个疗程,治疗组相同时间点QLQ-C30评分均低于对照组(P<0.05),但对照组QLQ-C30评分治疗前后比较,差异无统计学意义(P>0.05)。治疗1、2个疗程,2组CA19-9、CEA(对照组第1疗程CEA指标除外)、CYFRA21-1、CA125水平均较治疗前降低(P<0.05),且治疗组相同时间点4项指标均低于对照组(P<0.05)。治疗1、2个疗程,2组VIP、SOM、5-HT水平均较治疗前降低(P<0.05),MTL水平均较治疗前升高(P<0.05);且治疗组相同时间点VIP、SOM、5-HT水平均低于对照组(P<0.05),MTL水平均高于对照组(P<0.05)。治疗1、2个疗程,治疗组TGF-β_(1)RⅠ型受体、TGF-β_(1)RⅡ型受体、Smad4和Smad7水平均较治疗前降低(P<0.05),且相同时间点均低于对照组(P<0.05);对照组TGF-β_(1)RⅠ型受体、TGF-β_(1)RⅡ型受体、Smad7水平治疗前后差异均无统计学意义(P>0.05);对照组仅有Smad4水平于治疗2个疗程后明显低于治疗前(P<0.05)。随访3年,治疗组生存率为78.57%,对照组为56.10%,2组比较,差异有统计学意义(P<0.05)。治疗期间,治疗组骨髓抑制、皮肤毒性反应、消化道反应发生率低于对照组(P<0.05);2组膀胱、肾、心功能损伤发生率比较,差异无统计学意义(P>0.05)。结论:针刺联合穴位贴敷可明显提高中晚期食管癌放化疗患者的生存时间和生活质量,改善血清肿瘤标志物和胃肠激素水平,且安全性较高,其作用机制可能与调节TGF-β_(1)/Smad信号通路有关。

血清WISP1水平与绝经后骨质疏松症的相关性研究

目的:观察血清Wnt1诱导信号通路蛋白1(Wnt1-inducible-signaling pathway protein 1,WISP1)水平在绝经后骨质疏松症(postmenopausal osteoporosis,PMOP)患者中的变化,并分析其与骨密度(bone mineral density,BMD)各项骨代谢指标的相关性,探索WISP1在PMOP发生发展中的作用。方法:选取2020年5月─2022年8月在苏州大学附属第一医院健康管理中心进行健康体检的绝经后女性共148例,利用双能X线吸收仪(dual-energy X-ray absorptiometry,DXA)骨密度仪测量腰椎骨密度,根据不同骨量分为骨量正常组(T值≥-1.0),骨量减少组(-2.5<T值<-1.0),骨质疏松组(T值≤-2.5),分别采集临床基本资料,测定骨代谢指标及血清WISP1质量浓度,经SPSS软件进行统计处理。结果:骨质疏松组及骨量减少组的血清WISP1质量浓度均显著高于骨量正常组(均P<0.05)。血清WISP1水平与成骨标志物碱性磷酸酶、总Ⅰ型前胶原氨基端肽密切相关。结论:WISP1可能通过刺激成骨反应参与PMOP的病理生理过程。

Identification of F1 Cassava (<i>Manihot esculenta</i>Crantz) Progeny Using Microsatellite Markers and Capillary Electrophoresis

Generation of genetic diversity is necessary in improving on the potential of cassava when faced with various biotic and abiotic challenges. Presently, cassava breeders are breeding for a number of traits, such as drought tolerance, early root bulking, yield, starch, beta-carotene, protein, dry matter, pest and disease resistance, by relying on genetic diversity that exists in manihot esculenta germplasm. Controlled pollination is one of the main methods used to generate genetic diversity in cassava. However, the process of controlled pollination especially in an open field is prone to contamination by illegitimate pollen right from the time of pollination, seed collection, nursery bed establishment to planting of the trials. Therefore, authentication of the progeny obtained from cassava crosses is very important for genetic studies. Twelve informative microsatellite markers were used to verify the authenticity of 364 F1 progeny thought to come from four controlled parental crosses. The transmission of each allele at nine microsatellite loci was tracked from parents to progeny in each of the four Namikonga-derived F1 cassava families. Out of the 364 F1 progeny, 317 (87.1%) were true-to-type, 44 (12.1%) were a product of self-pollination and 3 (0.8%) were a product of open pollination. The consistency of the results obtained using microsatellite markers makes this technique a reliable tool for assessing the purity of progeny generated from cassava crosses.

Elevated soluble 4-1BB is associated with serum markers of hepatitis B virus in patients with chronic hepatitis B

BACKGROUND Previous studies have suggested that the costimulatory molecule 4-1BB plays pivotal roles in regulating immunity during chronic viral infection.However,up to now,there are few studies about 4-1BB in chronic hepatitis B(CHB).AIM To clarify this issue,we report our comprehensive study results on the expression levels of 4-1BB in patients with CHB.METHODS From September 2018 to June 2019,a total of 64 patients with CHB were recruited from the Department of Hepatology,The First Hospital of Jilin University.Peripheral blood samples were collected from 52 treatment-naïve and 12 entecavir-treated patients with CHB as well as 37 healthy donors(including 24 healthy adults and 13 healthy children).The levels of soluble 4-1BB(s4-1BB)in plasma were measured by ELISA.4-1BB mRNA expression in peripheral blood mononuclear cells was detected by real-time quantitative PCR.RESULTS The s4-1BB levels in the plasma of patients with CHB were significantly higher than those in healthy adults(94.390±7.393 ng/mL vs 8.875±0.914 ng/mL,P<0.001).In addition,the s4-1BB level in plasma was significantly increased in patients with a higher viral load and a disease flare up.However,there were no significant differences between treatment-naïve and entecavir-treated patients.Interestingly,among treatment-naïve patients with CHB,the levels of s4-1BB in plasma had a significant positive correlation with hepatitis B surface antigen,hepatitis B virus DNA,hepatitis B e antigen,and triglyceride levels(r=0.748,P<0.001;r=0.406,P=0.004;r=0.356,P=0.019 and r=-0.469,P=0.007,respectively).The 4-1BB mRNA expression was higher in the peripheral blood mononuclear cells of patients with CHB than in the peripheral blood mononuclear cells of healthy adults,but the difference was not statistically significant.CONCLUSION These results suggest that the levels of s4-1BB may be associated with pathogenesis of hepatitis B virus and therefore may be a promising biomarker for disease progression.