为探究des(rhamnosyl)verbascoside体外抗乙型肝炎病毒(HBV)的活性作用和机制,本研究以des(rhamnosyl)verbascoside为实验药物对HepG2.2.15细胞进行干预,实验分为药物干预组和对照组,采用串联质谱标签(Tandem Mass Tag,TMT)蛋白质组学...为探究des(rhamnosyl)verbascoside体外抗乙型肝炎病毒(HBV)的活性作用和机制,本研究以des(rhamnosyl)verbascoside为实验药物对HepG2.2.15细胞进行干预,实验分为药物干预组和对照组,采用串联质谱标签(Tandem Mass Tag,TMT)蛋白质组学方法对提取的总蛋白进行分析。结果表明,共筛选得到300个差异表达蛋白,其中有109个上调蛋白,191个下调蛋白。基因本体论(Gene Ontology,GO)分析结果显示,差异蛋白主要参与DNA复制(DNA replication)、鞘糖脂代谢(Glycosphingolipid metabolic process)、细胞增殖(Cell proliferation)、寡糖分解代谢(Oligosaccharide catabolic process)等生物学过程,以及DNA聚合酶活性(DNA polymerase activity)、丝氨酸型羧肽酶活性(Serine type carboxypeptidase activity)、DNA引物酶活性(DNA primase activity)等分子功能。京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)分析结果显示,差异蛋白主要参与细胞代谢(Metabolism)、遗传信息传导(Genetic information processing)、生物系统通路(Organismal systems)等相关信号通路。亚细胞定位分析表明,差异蛋白大多定位在细胞质和细胞核。本研究共筛选出13个与抗HBV密切相关的蛋白。通过定量蛋白组学初步揭示des(rhamnosyl)verbascoside可能通过增加HGF、SORT1、MAN2B1,减少PRIM1、PRIM2、POLA1、POLD3、POLD2、POLD1、POLE、ERCC2、LAMC1、SDC1等蛋白表达来起到体外抗HBV的作用。展开更多
[Objectives]This study was conducted to explore rapid and large-scale screening and detection of peste des petits ruminants(PPR),so as to provide important technical means for prevention,control and purification of PP...[Objectives]This study was conducted to explore rapid and large-scale screening and detection of peste des petits ruminants(PPR),so as to provide important technical means for prevention,control and purification of PPR.[Methods]Soluble N protein and NH fusion protein were successfully obtained in an Escherichia coli expression system by optimizing E.coli codon and expression conditions.Furthermore,based on purified soluble N protein and NH fusion protein,a double-antigen sandwich time-resolved fluorescence immunoassay method for detection of peste des petits ruminants virus(PPRV)was established.[Results]The method has high sensitivity and specificity and can specifically detect the antibody against PPRV in sheep serum,and it has no cross reaction with other related diseases.The method was used to detect 292 clinical samples,and compared with French IDVET competition ELISA kit.The coincidence rates of positive samples and negative samples from the two kinds of test kits were 92.47%and 97.26%,respectively,and the overall coincidence rate was 94.86%.The intra-group and inter-group coefficients of variation in the repeatability test were less than 10%.[Conclusions]Compared with the traditional ELISA method,the double-antigen sandwich time-resolved fluorescence immunoassay for detection of PPRV has equivalent sensitivity and specificity,and simple and rapid operation,and thus high application and popularization value.展开更多
To cultivate new professional farmers is a key way for rural labor development, resolving existing problems such as how to farming. It is notable that government and market take advantages in training of new professio...To cultivate new professional farmers is a key way for rural labor development, resolving existing problems such as how to farming. It is notable that government and market take advantages in training of new professional farmers. Therefore, it is necessary to guarantee government and market playing the roles. The research explored market-oriented farmer training model and the characteristics and investigated training routes for new professional farmers.展开更多
The impact of environmental regulation on technology innovation is a hot spot in current research where a large number of empirical studies are based on Porter Hypothesis(PH). However, there are still controversies in...The impact of environmental regulation on technology innovation is a hot spot in current research where a large number of empirical studies are based on Porter Hypothesis(PH). However, there are still controversies in academia about the establishment of "weak" and "narrow" versions of PH. Based on the panel data of application for patent of energy conservation and emission reduction(ECER) technology of Chinese city scale during 2008-2014, comprehensive energy price, pollutant emission, etc., mixed regression model and systematic generalized method of moments method were adopted, respectively,to study the impact of market-oriented and command-and-control policy tool on China's ECER technology innovation. The results show that the environmental regulation hindered the technological innovation in the immediate phase; however, it turned out to be positive in the first-lag phase. Hence, the establishment of "weak" PH is time-bounded. The command-and-control policy tool played a more positive role in promoting technological innovation in the first-lag phase than market-oriented policy tool. Therefore, "narrow" PH is not tenable. The reason is that the main participants of China's ECER technology innovation are state-owned companies and public institutions. Regionally speaking, the impact which command-and-control policy tool has on technological innovation at sight was nonsignificant in the eastern, the central, and the western regions of China whilst market-oriented policy tool had a negative effect. And market-oriented policy tool in the central region had strongest negative effect, which would diminish in the eastern region and become weakest in the western region. This was related to regional energy consumption level and the market economic vitality.展开更多
By analyzing the problems which exist currently in the accident hidden dangers management of the coal mine, this paper proposed a new kind of management method--"simulating the market", in which an operation pattern...By analyzing the problems which exist currently in the accident hidden dangers management of the coal mine, this paper proposed a new kind of management method--"simulating the market", in which an operation pattern of simulating the market to transact hidden troubles was constructed. This method introduces "Market Mechanism" into safe management, and adopts measurable value to describe the hidden dangers such as" human behavior, technique, environment, equipments etc.". It regards the hidden dangers as "the goods produced by labor" which are found out by the safety managers and the security inspectors, then sells as "commodity". By the process of disposing, counterchecking, re-selling, and redisposing. It forms a set of market-oriented closed-form management pattern of coalmine accident hidden dangers. This kind of management method changes the past traditional methods in which the wageworkers treat safety management passively, but to encourage and restrict them to participate in the check-up and improvement of the hidden dangers.展开更多
The purpose of this study is to analyze the characteristics of the spatial distribution and change trend of the marketing level of stateowned land supply so as to provide policy recommendations. Method of spatial auto...The purpose of this study is to analyze the characteristics of the spatial distribution and change trend of the marketing level of stateowned land supply so as to provide policy recommendations. Method of spatial autocorrelation analysis is employed. The results indicate that the spatial layout of the land supply marketing level is generally dispersed,but it does assemble in some specific area. The correlation between the marketing level of state-owned land supply and the economic development is not statistically significant. But their relations fluctuate obviously. The overall marketing level of state-owned land supply is increasing and spatially concentrated. The expansion rate of marketing has decreased first and then increased and stabilized now. The high-value cluster center of marketing level of state-owned land supply exists all over the country; however the existence probability of the cluster is bigger in undeveloped areas. It is concluded that spatial autocorrelation analysis is a good method to quantitatively analyze the spatial variation of marketing level of state-owned land supply in China. To grasp the spatial and temporal variations of the marketing level of state-owned land supply is also good to enhance running of the state-owned land market.展开更多
Not long ago, the Shanghai Port Machinery Co. Ltd. won the bid during international tender for the cement construction equipment for dams and factory buildings of Phase Ⅱ of the Three Gorges Project, thanks to its ex...Not long ago, the Shanghai Port Machinery Co. Ltd. won the bid during international tender for the cement construction equipment for dams and factory buildings of Phase Ⅱ of the Three Gorges Project, thanks to its excellent product quality, advanced technology content and fine enterprise image, obtaining the contract to build the world’s largest, technically difficult overhead cranes, fully displaying its tremendous strength.展开更多
This paper aims to explore the effects of market-oriented reforms on industrial technology progress.Based on a theoretical analysis,we performed an empirical study with a marketization index and panel data of high-tec...This paper aims to explore the effects of market-oriented reforms on industrial technology progress.Based on a theoretical analysis,we performed an empirical study with a marketization index and panel data of high-tech sectors in China.We found that market-oriented reforms had significantly propelled technology progress in China’s high-tech sectors,and the effects became more evident after China’s WTO entry.Market-oriented reforms induced technology progress by increasing capital allocation efficiency,R&D input,and technology diffusion.Among various aspects of market-oriented reforms,the institutional environment exerted the most significant effects,followed by the economy’s non-state sector,product market development,and factor market development;the government-market relationship index influenced technology’s progress the least.The effects are heterogeneous across sectors with different technology attributes and more significant for technology-intensive sectors.Our findings offer policy implications for China’s ongoing market-oriented reforms and policy design for technology progress in high-tech sectors.展开更多
The construction of the market-oriented ecological compensation mechanism in China is complicated system engineering. China's ecological compensation funds are mainly derived from the public finance, lacking marke...The construction of the market-oriented ecological compensation mechanism in China is complicated system engineering. China's ecological compensation funds are mainly derived from the public finance, lacking market-oriented operation. That not only increases the financial burden of the government, but also leads to the incomprehensive compensation scope. Moreover, China's ecological compensation lacks market mechanism so that it is difficult to set compensation standards and calculate offsets. This paper takes Gannan Tibetan Autonomous Prefecture as an example to analyze the market-oriented ecological compensation system of ethnic minority areas from the perspective of market economy, so as to provide a theoretical basis and a reference point for the establishment of efficient and reasonable ecological compensation mechanism and policies in ethnic minority areas and provide environmental protection for the sustainable development of economy and society of ethnic minority areas.展开更多
文摘为探究des(rhamnosyl)verbascoside体外抗乙型肝炎病毒(HBV)的活性作用和机制,本研究以des(rhamnosyl)verbascoside为实验药物对HepG2.2.15细胞进行干预,实验分为药物干预组和对照组,采用串联质谱标签(Tandem Mass Tag,TMT)蛋白质组学方法对提取的总蛋白进行分析。结果表明,共筛选得到300个差异表达蛋白,其中有109个上调蛋白,191个下调蛋白。基因本体论(Gene Ontology,GO)分析结果显示,差异蛋白主要参与DNA复制(DNA replication)、鞘糖脂代谢(Glycosphingolipid metabolic process)、细胞增殖(Cell proliferation)、寡糖分解代谢(Oligosaccharide catabolic process)等生物学过程,以及DNA聚合酶活性(DNA polymerase activity)、丝氨酸型羧肽酶活性(Serine type carboxypeptidase activity)、DNA引物酶活性(DNA primase activity)等分子功能。京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)分析结果显示,差异蛋白主要参与细胞代谢(Metabolism)、遗传信息传导(Genetic information processing)、生物系统通路(Organismal systems)等相关信号通路。亚细胞定位分析表明,差异蛋白大多定位在细胞质和细胞核。本研究共筛选出13个与抗HBV密切相关的蛋白。通过定量蛋白组学初步揭示des(rhamnosyl)verbascoside可能通过增加HGF、SORT1、MAN2B1,减少PRIM1、PRIM2、POLA1、POLD3、POLD2、POLD1、POLE、ERCC2、LAMC1、SDC1等蛋白表达来起到体外抗HBV的作用。
基金Supported by National Key R&D Program for the Prevention and Control of Major Exotic Animal Diseases(2022YFD1800500)National Mutton Sheep Industrial Technology System(CARS39)+2 种基金Key Research and Development Program of Shandong Province(Major Science and Technology Innovation Project)(2021CXGC011306)Scientific Research Project of General Administration of Customs(2024HK033)Scientific Research Project of Jinan Customs(2023JK005).
文摘[Objectives]This study was conducted to explore rapid and large-scale screening and detection of peste des petits ruminants(PPR),so as to provide important technical means for prevention,control and purification of PPR.[Methods]Soluble N protein and NH fusion protein were successfully obtained in an Escherichia coli expression system by optimizing E.coli codon and expression conditions.Furthermore,based on purified soluble N protein and NH fusion protein,a double-antigen sandwich time-resolved fluorescence immunoassay method for detection of peste des petits ruminants virus(PPRV)was established.[Results]The method has high sensitivity and specificity and can specifically detect the antibody against PPRV in sheep serum,and it has no cross reaction with other related diseases.The method was used to detect 292 clinical samples,and compared with French IDVET competition ELISA kit.The coincidence rates of positive samples and negative samples from the two kinds of test kits were 92.47%and 97.26%,respectively,and the overall coincidence rate was 94.86%.The intra-group and inter-group coefficients of variation in the repeatability test were less than 10%.[Conclusions]Compared with the traditional ELISA method,the double-antigen sandwich time-resolved fluorescence immunoassay for detection of PPRV has equivalent sensitivity and specificity,and simple and rapid operation,and thus high application and popularization value.
基金Supported by Chongqing Education Science Planning Program(2013-ZJ-060)Humanities and Social Science Research Planning Program of Ministry of Education(13YJA630042)+1 种基金Humanities and Social Science Research Program of Chongqing Education Committee(14SKN03)S&T Innovation Team Construction and Planning Foundation of Yangtze Normal University(2014XJTD03)~~
文摘To cultivate new professional farmers is a key way for rural labor development, resolving existing problems such as how to farming. It is notable that government and market take advantages in training of new professional farmers. Therefore, it is necessary to guarantee government and market playing the roles. The research explored market-oriented farmer training model and the characteristics and investigated training routes for new professional farmers.
文摘The impact of environmental regulation on technology innovation is a hot spot in current research where a large number of empirical studies are based on Porter Hypothesis(PH). However, there are still controversies in academia about the establishment of "weak" and "narrow" versions of PH. Based on the panel data of application for patent of energy conservation and emission reduction(ECER) technology of Chinese city scale during 2008-2014, comprehensive energy price, pollutant emission, etc., mixed regression model and systematic generalized method of moments method were adopted, respectively,to study the impact of market-oriented and command-and-control policy tool on China's ECER technology innovation. The results show that the environmental regulation hindered the technological innovation in the immediate phase; however, it turned out to be positive in the first-lag phase. Hence, the establishment of "weak" PH is time-bounded. The command-and-control policy tool played a more positive role in promoting technological innovation in the first-lag phase than market-oriented policy tool. Therefore, "narrow" PH is not tenable. The reason is that the main participants of China's ECER technology innovation are state-owned companies and public institutions. Regionally speaking, the impact which command-and-control policy tool has on technological innovation at sight was nonsignificant in the eastern, the central, and the western regions of China whilst market-oriented policy tool had a negative effect. And market-oriented policy tool in the central region had strongest negative effect, which would diminish in the eastern region and become weakest in the western region. This was related to regional energy consumption level and the market economic vitality.
文摘By analyzing the problems which exist currently in the accident hidden dangers management of the coal mine, this paper proposed a new kind of management method--"simulating the market", in which an operation pattern of simulating the market to transact hidden troubles was constructed. This method introduces "Market Mechanism" into safe management, and adopts measurable value to describe the hidden dangers such as" human behavior, technique, environment, equipments etc.". It regards the hidden dangers as "the goods produced by labor" which are found out by the safety managers and the security inspectors, then sells as "commodity". By the process of disposing, counterchecking, re-selling, and redisposing. It forms a set of market-oriented closed-form management pattern of coalmine accident hidden dangers. This kind of management method changes the past traditional methods in which the wageworkers treat safety management passively, but to encourage and restrict them to participate in the check-up and improvement of the hidden dangers.
基金Supported by Chongqing Key Humanities and Social Sciences Base--Research Center of Rural Economics and Management of Southwest University
文摘The purpose of this study is to analyze the characteristics of the spatial distribution and change trend of the marketing level of stateowned land supply so as to provide policy recommendations. Method of spatial autocorrelation analysis is employed. The results indicate that the spatial layout of the land supply marketing level is generally dispersed,but it does assemble in some specific area. The correlation between the marketing level of state-owned land supply and the economic development is not statistically significant. But their relations fluctuate obviously. The overall marketing level of state-owned land supply is increasing and spatially concentrated. The expansion rate of marketing has decreased first and then increased and stabilized now. The high-value cluster center of marketing level of state-owned land supply exists all over the country; however the existence probability of the cluster is bigger in undeveloped areas. It is concluded that spatial autocorrelation analysis is a good method to quantitatively analyze the spatial variation of marketing level of state-owned land supply in China. To grasp the spatial and temporal variations of the marketing level of state-owned land supply is also good to enhance running of the state-owned land market.
文摘Not long ago, the Shanghai Port Machinery Co. Ltd. won the bid during international tender for the cement construction equipment for dams and factory buildings of Phase Ⅱ of the Three Gorges Project, thanks to its excellent product quality, advanced technology content and fine enterprise image, obtaining the contract to build the world’s largest, technically difficult overhead cranes, fully displaying its tremendous strength.
基金by the General Program of the National Science Foundation of China(NSFC)“Study on the Effects of Factor Price Distortion on the Technology Sophistication of Exports from High-tech Sectors and Policy Response”(Grant No.71773107).
文摘This paper aims to explore the effects of market-oriented reforms on industrial technology progress.Based on a theoretical analysis,we performed an empirical study with a marketization index and panel data of high-tech sectors in China.We found that market-oriented reforms had significantly propelled technology progress in China’s high-tech sectors,and the effects became more evident after China’s WTO entry.Market-oriented reforms induced technology progress by increasing capital allocation efficiency,R&D input,and technology diffusion.Among various aspects of market-oriented reforms,the institutional environment exerted the most significant effects,followed by the economy’s non-state sector,product market development,and factor market development;the government-market relationship index influenced technology’s progress the least.The effects are heterogeneous across sectors with different technology attributes and more significant for technology-intensive sectors.Our findings offer policy implications for China’s ongoing market-oriented reforms and policy design for technology progress in high-tech sectors.
文摘The construction of the market-oriented ecological compensation mechanism in China is complicated system engineering. China's ecological compensation funds are mainly derived from the public finance, lacking market-oriented operation. That not only increases the financial burden of the government, but also leads to the incomprehensive compensation scope. Moreover, China's ecological compensation lacks market mechanism so that it is difficult to set compensation standards and calculate offsets. This paper takes Gannan Tibetan Autonomous Prefecture as an example to analyze the market-oriented ecological compensation system of ethnic minority areas from the perspective of market economy, so as to provide a theoretical basis and a reference point for the establishment of efficient and reasonable ecological compensation mechanism and policies in ethnic minority areas and provide environmental protection for the sustainable development of economy and society of ethnic minority areas.