The viability of a complete structural characterization of civil structures is explored and discussed. In particular, the identification of modal （i.e. natural frequencies, damping ratios and modal shapes） and physi...The viability of a complete structural characterization of civil structures is explored and discussed. In particular, the identification of modal （i.e. natural frequencies, damping ratios and modal shapes） and physical properties （i.e. mass and stiffness） using only the structure＇s free decay response is studied. To accomplish this, modal analysis from flee vibration response only （MAFVRO） and mass modification （MM） methodologies are engaged along with Wavelet based techniques for optimal signal processing and modal reconstruction. The methodologies are evaluated using simulated and experimental data. The simulated data are extracted from a simple elastic model of a 5 story shear building and from a more realistic nonlinear model of a RC frame structure. The experimental data are gathered from shake table test of a 2-story scaled shear building. Guidelines for the reconstruction procedure from the data are proposed as the quality of the identified properties is shown to be governed by adequate selection of the frequency bands and optimal modal shape reconstruction. Moreover, in cases where the structure has undergone damage, the proposed identification scheme can also be applied for preliminary assessment of structural health.展开更多
Active endogenous metabolites regulate the viability of cells. This process is controlled by a series ofinteractions between small metabolites and large proteins. Previously, several studies had reported thatmetabolit...Active endogenous metabolites regulate the viability of cells. This process is controlled by a series ofinteractions between small metabolites and large proteins. Previously, several studies had reported thatmetabolite regulates the protein functions, such as diacylglycerol to protein kinase C, lactose regulationof the lac repressor, and HIF-1α stabilization by 2-hydroxyglutarate. However, decades old traditionalbiochemical methods are insufficient to systematically investigate the bio-molecular reactions for a high-throughput discovery. Here, we have reviewed an update on the recently developed chemical proteomicscalled activity-based protein profiling （ABPP）. ABPP is able to identify proteins interacted eithercovalently or non-covalently with metabolites significantly. Thus, ABPP will facilitate the characteriza-tion of specific metabolite regulating; proteins in human disease progression.展开更多
基金supported by the National Science Foundation Grant No.CMMI-1121146
文摘The viability of a complete structural characterization of civil structures is explored and discussed. In particular, the identification of modal （i.e. natural frequencies, damping ratios and modal shapes） and physical properties （i.e. mass and stiffness） using only the structure＇s free decay response is studied. To accomplish this, modal analysis from flee vibration response only （MAFVRO） and mass modification （MM） methodologies are engaged along with Wavelet based techniques for optimal signal processing and modal reconstruction. The methodologies are evaluated using simulated and experimental data. The simulated data are extracted from a simple elastic model of a 5 story shear building and from a more realistic nonlinear model of a RC frame structure. The experimental data are gathered from shake table test of a 2-story scaled shear building. Guidelines for the reconstruction procedure from the data are proposed as the quality of the identified properties is shown to be governed by adequate selection of the frequency bands and optimal modal shape reconstruction. Moreover, in cases where the structure has undergone damage, the proposed identification scheme can also be applied for preliminary assessment of structural health.
基金supported by the National Natural Science Foundation of China(No.81672440)Innovation Program of Science and Research from the DICP,CAS(No.DICP TMSR201601)the 100 Talents Program of Chinese Academy of Sciences
文摘Active endogenous metabolites regulate the viability of cells. This process is controlled by a series ofinteractions between small metabolites and large proteins. Previously, several studies had reported thatmetabolite regulates the protein functions, such as diacylglycerol to protein kinase C, lactose regulationof the lac repressor, and HIF-1α stabilization by 2-hydroxyglutarate. However, decades old traditionalbiochemical methods are insufficient to systematically investigate the bio-molecular reactions for a high-throughput discovery. Here, we have reviewed an update on the recently developed chemical proteomicscalled activity-based protein profiling （ABPP）. ABPP is able to identify proteins interacted eithercovalently or non-covalently with metabolites significantly. Thus, ABPP will facilitate the characteriza-tion of specific metabolite regulating; proteins in human disease progression.
