[Objectives]To study the antibacterial effects of extracts from Pinus massoniana Lamb.needles.[Methods]In this experiment,the components from Pinus massoniana Lamb.needles were extracted by systematic solvent extracti...[Objectives]To study the antibacterial effects of extracts from Pinus massoniana Lamb.needles.[Methods]In this experiment,the components from Pinus massoniana Lamb.needles were extracted by systematic solvent extraction method,and the antibacterial activity against four common bacteria,Escherichia coli,Staphylococcus aureus,Bacillus subtilis,Aspergillus flavus and the antibacterial active component were examined for by punch method.[Results]Different solvent extraction rate was different,the rates of petroleum ether,chloroform,ethyl acetate,n-butanol,water extracts were 4.2%,16.7%,17.4%,21.1%,40.6%.All extracts showed inhibitory effect against test bacteria.It was observed that the inhibition of G+was stronger than G-,and the extracts had the best antibacterial activity to Staphylococcus aureus while the weakest to Aspergillus flavus.The antibacterial activity of the components decreased in the order:ethyl acetate extract>n-butanol extract>chloroform extract>petroleum ether extract>aqueous phase.The extracts were stable under ultraviolet radiation(UV)light and long term storage.The antibacterial activity of the extracts was weaker with the increase of pH value when the pH value≤8.[Conclusions]It is inferred that the antibacterial components in the extract of Pinus massoniana needles are widely distributed,and the components with medium polarity or above are the main antibacterial components.展开更多
Chitosan oligosaccharides(COSs)are the main degradation products from chitosan or chitin and have been reported to induce resistance to diseases in herbaceous plants like cucumber and Arabidopsis.Concomitantly,pine wi...Chitosan oligosaccharides(COSs)are the main degradation products from chitosan or chitin and have been reported to induce resistance to diseases in herbaceous plants like cucumber and Arabidopsis.Concomitantly,pine wilt disease(PWD)is a devastating disease of conifer tree species.Here,we hypothesized that COSs induce plant resistance gene(PRG)expression in the woody plant Masson pine,Pinus massoniana.COSs were inoculated into P.massoniana seedlings and the BGISEQ-500 platform was used to generate transcriptomes from COSs-treated P.massoniana and control seedlings.A total of 501 differentially expressed genes(DEGs)were identified by comparing the treatment and control groups.A total of 251(50.1%)DEGs were up-regulated in the treatment relative to the control seedlings and 250(49.9%)were down-regulated.Inoculation of COSs induced the expression of 31 PRGs in P.massoniana seedlings and the relative expression levels of six of the PRGs were verified by RT-qPCR.This is the first study to demonstrate that COS induces the expression of PRGs in a tree species.These results provide important insights into the function of COSs and further the prospects of developing a COS-based immune inducer for controlling PWD.展开更多
基金Supported by Zhanjiang Non-funded Science and Technology Research Plan in 2023(2023B01023)School-level Education and Teaching Reform Project of Lingnan Normal University in 2022(LingShiJiaoWu2022154).
文摘[Objectives]To study the antibacterial effects of extracts from Pinus massoniana Lamb.needles.[Methods]In this experiment,the components from Pinus massoniana Lamb.needles were extracted by systematic solvent extraction method,and the antibacterial activity against four common bacteria,Escherichia coli,Staphylococcus aureus,Bacillus subtilis,Aspergillus flavus and the antibacterial active component were examined for by punch method.[Results]Different solvent extraction rate was different,the rates of petroleum ether,chloroform,ethyl acetate,n-butanol,water extracts were 4.2%,16.7%,17.4%,21.1%,40.6%.All extracts showed inhibitory effect against test bacteria.It was observed that the inhibition of G+was stronger than G-,and the extracts had the best antibacterial activity to Staphylococcus aureus while the weakest to Aspergillus flavus.The antibacterial activity of the components decreased in the order:ethyl acetate extract>n-butanol extract>chloroform extract>petroleum ether extract>aqueous phase.The extracts were stable under ultraviolet radiation(UV)light and long term storage.The antibacterial activity of the extracts was weaker with the increase of pH value when the pH value≤8.[Conclusions]It is inferred that the antibacterial components in the extract of Pinus massoniana needles are widely distributed,and the components with medium polarity or above are the main antibacterial components.
文摘Chitosan oligosaccharides(COSs)are the main degradation products from chitosan or chitin and have been reported to induce resistance to diseases in herbaceous plants like cucumber and Arabidopsis.Concomitantly,pine wilt disease(PWD)is a devastating disease of conifer tree species.Here,we hypothesized that COSs induce plant resistance gene(PRG)expression in the woody plant Masson pine,Pinus massoniana.COSs were inoculated into P.massoniana seedlings and the BGISEQ-500 platform was used to generate transcriptomes from COSs-treated P.massoniana and control seedlings.A total of 501 differentially expressed genes(DEGs)were identified by comparing the treatment and control groups.A total of 251(50.1%)DEGs were up-regulated in the treatment relative to the control seedlings and 250(49.9%)were down-regulated.Inoculation of COSs induced the expression of 31 PRGs in P.massoniana seedlings and the relative expression levels of six of the PRGs were verified by RT-qPCR.This is the first study to demonstrate that COS induces the expression of PRGs in a tree species.These results provide important insights into the function of COSs and further the prospects of developing a COS-based immune inducer for controlling PWD.
