In order to develop an efficient identification method for Fallopia plants and drugs,molecular analysis of the partial matK gene sequences was performed on 6 Fallopia species.Based on the matK sequences,a phylogenetic...In order to develop an efficient identification method for Fallopia plants and drugs,molecular analysis of the partial matK gene sequences was performed on 6 Fallopia species.Based on the matK sequences,a phylogenetic tree was constructed,which showed that different populations of inter-and intra-species could be specified and distinguished.The matK gene sequences of the 6 Fallopia species were all found to be of 1 271 bp in length,with some nucleotide variations throughout the entire sequences.The nucleotide difference at position 1 041 could distinguish F.denticulata from others,while specific nucleotide at position 1 154 became identification markers for F.aubertii.Moreover,four specific marker sites for F.multiflora var.ciliinerve at positions 216,224,1 060 and 1 179,seven for F.convolvulus at 318,765,772,874,936,952 and 1 036,and seven for F.dentate alata at 111,192,366,450,457,1 032 and 1 074 were also observed.By detecting the marker nucleotides and analyzing the phylogenetic relationship,the botanical origins of five inspected drugs were determined,suggesting that matK sequences can be used for authenticating Fallopia plants and drugs.展开更多
The chloroplast gene matK and the internal transcribed spacers (ITS) of the nuclear ribosomal DNA from Schnabelia, a genus endemic to China, and 6 genera of Verbenaceae and 13 genera of Lamiaceae were sequenced. The p...The chloroplast gene matK and the internal transcribed spacers (ITS) of the nuclear ribosomal DNA from Schnabelia, a genus endemic to China, and 6 genera of Verbenaceae and 13 genera of Lamiaceae were sequenced. The phylogenetic signal and validity outgroups were measured and evaluated by means of the relatively apparent synapomorphy analysis (RASA). Independent and combined phylogenetic analyses for the matK and ITS sequences were performed using the maximum parsimony (MP), neighbor- joining (NJ) and maximum likelihood (ML) methods, indicating that Schnabelia oligophylla and Caryopteris terniflora form a sister-group relationship. The Caryopteris complex is not shown to be a monophyly because Trichostema, C. paniculata and C. forrestii are paraphyletic to the clade containing the remaining members of the complex. A monophyly of Ajugoideae proposed by Cantino et al., including 8 genera in this study, is strongly supported and the closest relatives of Schnabelia are in the Ajugoideae (Lamiaceae), especially near Caryopteris terniflora. The polygenetic analyses also showed that the genera of Lamiaceae and Verbenaceae sampled in this tudy are phylogenetically mixed and the genus Avicennia is distant to other genera of Verbenaceae. RASA and combined analysis can be used as effective approaches to determining the relationships among phylogenetically complex groups.展开更多
目的 比较几种药典收载石斛与常见市场混淆品种的 mat K基因序列 ,为石斛类药材的鉴定提供分子依据。方法 PCR扩增、测序、PAUP软件分析。结果 非石斛属的几种混淆品与正品石斛间的 mat K基因序列差异远大于正品石斛间的差异 ,PAUP4....目的 比较几种药典收载石斛与常见市场混淆品种的 mat K基因序列 ,为石斛类药材的鉴定提供分子依据。方法 PCR扩增、测序、PAUP软件分析。结果 非石斛属的几种混淆品与正品石斛间的 mat K基因序列差异远大于正品石斛间的差异 ,PAUP4.0构建的树型图亦显示几种石斛属植物聚在一起 ,而非石斛属植物聚在外方。结论 通过 mat K基因序列可以分析石斛及其混淆品间的遗传关系 ,将正品与混淆品区别开来。展开更多
基金Supported by the Natural Science Foundation of Guangdong Province(06019716)
文摘In order to develop an efficient identification method for Fallopia plants and drugs,molecular analysis of the partial matK gene sequences was performed on 6 Fallopia species.Based on the matK sequences,a phylogenetic tree was constructed,which showed that different populations of inter-and intra-species could be specified and distinguished.The matK gene sequences of the 6 Fallopia species were all found to be of 1 271 bp in length,with some nucleotide variations throughout the entire sequences.The nucleotide difference at position 1 041 could distinguish F.denticulata from others,while specific nucleotide at position 1 154 became identification markers for F.aubertii.Moreover,four specific marker sites for F.multiflora var.ciliinerve at positions 216,224,1 060 and 1 179,seven for F.convolvulus at 318,765,772,874,936,952 and 1 036,and seven for F.dentate alata at 111,192,366,450,457,1 032 and 1 074 were also observed.By detecting the marker nucleotides and analyzing the phylogenetic relationship,the botanical origins of five inspected drugs were determined,suggesting that matK sequences can be used for authenticating Fallopia plants and drugs.
基金supported by funds from the Arnold Arboretum of Harvard University for fieldwork in the U.S.A.the National Science Foundation for Distinguished Young Scholars(Grant No.39825104)+2 种基金the National Natural Science Foundation of China(Grant Nos.30070053,30170071 and 30230030)the Natural Science Foundation of Guangdong Province(Grant No.001223)the Ministry of Education Special Foundation(Grant No.20010558013).
文摘The chloroplast gene matK and the internal transcribed spacers (ITS) of the nuclear ribosomal DNA from Schnabelia, a genus endemic to China, and 6 genera of Verbenaceae and 13 genera of Lamiaceae were sequenced. The phylogenetic signal and validity outgroups were measured and evaluated by means of the relatively apparent synapomorphy analysis (RASA). Independent and combined phylogenetic analyses for the matK and ITS sequences were performed using the maximum parsimony (MP), neighbor- joining (NJ) and maximum likelihood (ML) methods, indicating that Schnabelia oligophylla and Caryopteris terniflora form a sister-group relationship. The Caryopteris complex is not shown to be a monophyly because Trichostema, C. paniculata and C. forrestii are paraphyletic to the clade containing the remaining members of the complex. A monophyly of Ajugoideae proposed by Cantino et al., including 8 genera in this study, is strongly supported and the closest relatives of Schnabelia are in the Ajugoideae (Lamiaceae), especially near Caryopteris terniflora. The polygenetic analyses also showed that the genera of Lamiaceae and Verbenaceae sampled in this tudy are phylogenetically mixed and the genus Avicennia is distant to other genera of Verbenaceae. RASA and combined analysis can be used as effective approaches to determining the relationships among phylogenetically complex groups.
文摘目的 比较几种药典收载石斛与常见市场混淆品种的 mat K基因序列 ,为石斛类药材的鉴定提供分子依据。方法 PCR扩增、测序、PAUP软件分析。结果 非石斛属的几种混淆品与正品石斛间的 mat K基因序列差异远大于正品石斛间的差异 ,PAUP4.0构建的树型图亦显示几种石斛属植物聚在一起 ,而非石斛属植物聚在外方。结论 通过 mat K基因序列可以分析石斛及其混淆品间的遗传关系 ,将正品与混淆品区别开来。
