Social anxiety (SA) is a prevalent mental health issue among adolescents, and vulnerable narcissism (VN) can exacerbate thiscondition. This study aims to investigate the impact of vulnerable narcissism on social anxie...Social anxiety (SA) is a prevalent mental health issue among adolescents, and vulnerable narcissism (VN) can exacerbate thiscondition. This study aims to investigate the impact of vulnerable narcissism on social anxiety in adolescents, specificallyfocusing on the mediating effects of self-concept clarity (SCC) and self-esteem (SE) in the relationship between vulnerablenarcissism and social anxiety. Through cluster sampling, a questionnaire survey was conducted among 982 students from threesecondary schools in two provinces. The data was analyzed using structural equation modeling (SEM). The results revealedthat there was a significant negative correlation between vulnerable narcissism and both self-concept clarity and self-esteem,while there was a significant positive correlation between vulnerable narcissism and social anxiety. Additionally, self-conceptclarity showed a significant positive correlation with self-esteem but had a negative correlation with social anxiety. Both selfconceptclarity and self-esteem played an intermediary role in the chain linking vulnerable narcissism to social anxiety. Thisstudy confirms the mediating role of both self-concept clarity and self-esteem in explaining how vulnerable narcissisminfluences social anxiety, providing valuable insights into its underlying mechanism.展开更多
RISC-V指令集架构(Instruction Set Architecture,ISA)作为一种新兴的精简ISA,因免费、开源、自由等特点而得到快速发展.由于国内外对RISC-V的研究主要集中在硬件开发,软件生态相较于成熟ISA还很薄弱,实现一套RISC-V指令集高性能基础数...RISC-V指令集架构(Instruction Set Architecture,ISA)作为一种新兴的精简ISA,因免费、开源、自由等特点而得到快速发展.由于国内外对RISC-V的研究主要集中在硬件开发,软件生态相较于成熟ISA还很薄弱,实现一套RISC-V指令集高性能基础数学库可以进一步丰富RISC-V软件生态.本文基于自动化移植技术实现申威数学库到RISC-V的移植,为RISC-V指令架构提供首个使用向量指令优化的基础数学库系统.本文提出向量寄存器自动分支查表法与路径标记插入法,重点解决不同架构间寄存器映射过程中的寄存器复用问题,实现寄存器正确高效映射,并依据不同指令等价转换策略自动化移植数学函数69个.测试结果表明,RISC-V基础数学库函数可实现正确计算,最大误差为1.90ULP,函数性能平均为157.03节拍.展开更多
目的研究腺病毒携带Math1-EGFP基因经完整圆窗膜途径及鼓阶打孔途径导入耳蜗后对听功能和转导效率的影响,为内耳基因治疗提供实验基础和理论依据。方法健康成年白色红目豚鼠40只,雌雄不限,体重250～300g。随机分成四组,完整圆窗膜组12只...目的研究腺病毒携带Math1-EGFP基因经完整圆窗膜途径及鼓阶打孔途径导入耳蜗后对听功能和转导效率的影响,为内耳基因治疗提供实验基础和理论依据。方法健康成年白色红目豚鼠40只,雌雄不限,体重250～300g。随机分成四组,完整圆窗膜组12只,鼓阶打孔组12只,各组分别设对照8只。实验组(24只)导入重组腺病毒携带的Math1基因及增强型绿色荧光蛋白基因(enhanced green fluorescent protein,EGFP),对照组(16只)导入人工外淋巴液,所有动物均以左耳作为导入耳。术前及术后分别行听性脑干反应(ABR)检查。分别于术后5天、14天取双侧耳蜗标本做基底膜铺片观察基因表达情况。结果完整圆窗膜组导入耳ABR阈值,术后5天各频率与术前比较无显著性差异(P>0.05);鼓阶打孔组导入耳ABR阈值,术后5天在2kHz、4kHz与术前比较无差异(P>0.05),8kHz较术前增高(P<0.05),16kHz、20kHz较术前明显增高(P<0.01),术后14天在16kHz、20kHz较术后5天时明显好转(P<0.01),但较术前仍有增高(P<0.05)。转导成功率鼓阶打孔组为91.6%,优于完整圆窗膜组的50%。两种转导途径对目的基因在耳蜗内的表达部位和表达时间没有显著影响。结论完整圆窗膜途径及鼓阶打孔途径在转导成功率及听功能保护方面各有优劣。完整圆窗膜途径因其对耳蜗的损伤极小,在临床应用方面具有更好的发展前景。展开更多
目的研究以复制缺陷型腺病毒为载体的Math1基因内耳应用的安全性。方法将10只成年Wistar大鼠分为正常对照组和缺失E1、E3基因片段且构建有Math1基因和绿色荧光蛋白报告基因的复制缺陷型腺病毒(adenovirus-Math1-enhanced green fluoresc...目的研究以复制缺陷型腺病毒为载体的Math1基因内耳应用的安全性。方法将10只成年Wistar大鼠分为正常对照组和缺失E1、E3基因片段且构建有Math1基因和绿色荧光蛋白报告基因的复制缺陷型腺病毒(adenovirus-Math1-enhanced green fluorescence protein,Ad-Math1-EGFP)前庭阶导入组(实验组),每组5只,实验组大鼠在右耳通过耳蜗底回前庭阶打孔的方法导入物理滴度为2.1×1011v.p/ml的上述腺病毒5μl,对照组大鼠不做任何处理。7天后对动物进行颈髓硬膜外短声诱发电位(click-evoked potentials on the surface of the cervical dura mater,CDM-CEP)、听性脑干反应(ABR)阈值检测和游泳试验,评价前庭和耳蜗功能,然后将动物处死进行组织形态学观察。结果Ad-Math1-EGFP导入7天后,实验组大鼠前庭及耳蜗的毛细胞及纤毛均未见破坏,形态正常。短声刺激下,对照组大鼠CDM-CEP的阈值为85±3.54dBSPL,ABR阈值为37±4.47dBSPL;实验组大鼠CDM-CEP的阈值为89±6.52dBSPL,ABR阈值为40±3.54dBSPL;对照组大鼠的游泳时间为4.0±0.71s,实验组为5.0±0.71s,两组之间比较差异均无统计学意义。结论携带Math1基因的复制缺陷型腺病毒对前庭和耳蜗毛细胞是安全的,可以作为基因导入的理想载体。展开更多
Aim The particle texture from diesel engine was imitated by use of computer. Methods The theory of fractal geometry and the diffusion limited aggregation model were used to simulate the micron texture. Results The...Aim The particle texture from diesel engine was imitated by use of computer. Methods The theory of fractal geometry and the diffusion limited aggregation model were used to simulate the micron texture. Results The fractal dimensions of granule distribution and corpuscle superficial area are quite conformed with those of measurement. Conclusion The texture parameters of engine particle cluster can be obtained precisely by use of fractal theory.展开更多
目的比较腺病毒、脂质体、纳米材料三种不同类型的载体体外携带目的基因Math1对HEK293T细胞的转染效率及细胞毒性的大小,以期筛选理想的基因转染载体材料。方法选用重组腺病毒、LipofectamineTM2000、Superfect纳米材料作为转染载体,携...目的比较腺病毒、脂质体、纳米材料三种不同类型的载体体外携带目的基因Math1对HEK293T细胞的转染效率及细胞毒性的大小,以期筛选理想的基因转染载体材料。方法选用重组腺病毒、LipofectamineTM2000、Superfect纳米材料作为转染载体,携带含有增强型绿色荧光蛋白(enhanced green fluorescent protein,EGFP)报告基因的Math1-EGFP基因及真核表达质粒pRK5-Math1-EGFP,并且根据不同转染载体说明步骤优化体外转染条件,分别转染293T细胞。在一定的时间内利用荧光显微镜观察细胞转染结果并计数阳性细胞,采用MTT(噻唑蓝)法检测三种不同载体体外细胞毒性,应用RT-PCR(逆转录聚合酶链反应)技术检测转染阳性细胞中目的基因Math1的mRNA表达情况。结果在优化的体外转染条件下,重组腺病毒载体介导的细胞转染率达到了94%以上,脂质体和商品化Superfect纳米材料转染的细胞中,荧光蛋白表达率分别为73%和80%以上,同时,商品化Superfect纳米材料在达到80%转染率的条件下,细胞存活率为90%。应用RT-PCR方法证实,三种载体转染细胞均有Math1基因的mRNA的表达。结论商品化Superfect纳米材料作为一种新型的、安全有效的纳米转染材料,能够成功携带耳聋治疗关键基因Math1转染293T细胞,以期在内耳基因治疗的研究当中得到有效的应用。展开更多
基金the National Natural Science Foundation of China(31960181,32360213 and 82260364).
文摘Social anxiety (SA) is a prevalent mental health issue among adolescents, and vulnerable narcissism (VN) can exacerbate thiscondition. This study aims to investigate the impact of vulnerable narcissism on social anxiety in adolescents, specificallyfocusing on the mediating effects of self-concept clarity (SCC) and self-esteem (SE) in the relationship between vulnerablenarcissism and social anxiety. Through cluster sampling, a questionnaire survey was conducted among 982 students from threesecondary schools in two provinces. The data was analyzed using structural equation modeling (SEM). The results revealedthat there was a significant negative correlation between vulnerable narcissism and both self-concept clarity and self-esteem,while there was a significant positive correlation between vulnerable narcissism and social anxiety. Additionally, self-conceptclarity showed a significant positive correlation with self-esteem but had a negative correlation with social anxiety. Both selfconceptclarity and self-esteem played an intermediary role in the chain linking vulnerable narcissism to social anxiety. Thisstudy confirms the mediating role of both self-concept clarity and self-esteem in explaining how vulnerable narcissisminfluences social anxiety, providing valuable insights into its underlying mechanism.
文摘RISC-V指令集架构(Instruction Set Architecture,ISA)作为一种新兴的精简ISA,因免费、开源、自由等特点而得到快速发展.由于国内外对RISC-V的研究主要集中在硬件开发,软件生态相较于成熟ISA还很薄弱,实现一套RISC-V指令集高性能基础数学库可以进一步丰富RISC-V软件生态.本文基于自动化移植技术实现申威数学库到RISC-V的移植,为RISC-V指令架构提供首个使用向量指令优化的基础数学库系统.本文提出向量寄存器自动分支查表法与路径标记插入法,重点解决不同架构间寄存器映射过程中的寄存器复用问题,实现寄存器正确高效映射,并依据不同指令等价转换策略自动化移植数学函数69个.测试结果表明,RISC-V基础数学库函数可实现正确计算,最大误差为1.90ULP,函数性能平均为157.03节拍.
文摘目的研究腺病毒携带Math1-EGFP基因经完整圆窗膜途径及鼓阶打孔途径导入耳蜗后对听功能和转导效率的影响,为内耳基因治疗提供实验基础和理论依据。方法健康成年白色红目豚鼠40只,雌雄不限,体重250～300g。随机分成四组,完整圆窗膜组12只,鼓阶打孔组12只,各组分别设对照8只。实验组(24只)导入重组腺病毒携带的Math1基因及增强型绿色荧光蛋白基因(enhanced green fluorescent protein,EGFP),对照组(16只)导入人工外淋巴液,所有动物均以左耳作为导入耳。术前及术后分别行听性脑干反应(ABR)检查。分别于术后5天、14天取双侧耳蜗标本做基底膜铺片观察基因表达情况。结果完整圆窗膜组导入耳ABR阈值,术后5天各频率与术前比较无显著性差异(P>0.05);鼓阶打孔组导入耳ABR阈值,术后5天在2kHz、4kHz与术前比较无差异(P>0.05),8kHz较术前增高(P<0.05),16kHz、20kHz较术前明显增高(P<0.01),术后14天在16kHz、20kHz较术后5天时明显好转(P<0.01),但较术前仍有增高(P<0.05)。转导成功率鼓阶打孔组为91.6%,优于完整圆窗膜组的50%。两种转导途径对目的基因在耳蜗内的表达部位和表达时间没有显著影响。结论完整圆窗膜途径及鼓阶打孔途径在转导成功率及听功能保护方面各有优劣。完整圆窗膜途径因其对耳蜗的损伤极小,在临床应用方面具有更好的发展前景。
文摘目的研究以复制缺陷型腺病毒为载体的Math1基因内耳应用的安全性。方法将10只成年Wistar大鼠分为正常对照组和缺失E1、E3基因片段且构建有Math1基因和绿色荧光蛋白报告基因的复制缺陷型腺病毒(adenovirus-Math1-enhanced green fluorescence protein,Ad-Math1-EGFP)前庭阶导入组(实验组),每组5只,实验组大鼠在右耳通过耳蜗底回前庭阶打孔的方法导入物理滴度为2.1×1011v.p/ml的上述腺病毒5μl,对照组大鼠不做任何处理。7天后对动物进行颈髓硬膜外短声诱发电位(click-evoked potentials on the surface of the cervical dura mater,CDM-CEP)、听性脑干反应(ABR)阈值检测和游泳试验,评价前庭和耳蜗功能,然后将动物处死进行组织形态学观察。结果Ad-Math1-EGFP导入7天后,实验组大鼠前庭及耳蜗的毛细胞及纤毛均未见破坏,形态正常。短声刺激下,对照组大鼠CDM-CEP的阈值为85±3.54dBSPL,ABR阈值为37±4.47dBSPL;实验组大鼠CDM-CEP的阈值为89±6.52dBSPL,ABR阈值为40±3.54dBSPL;对照组大鼠的游泳时间为4.0±0.71s,实验组为5.0±0.71s,两组之间比较差异均无统计学意义。结论携带Math1基因的复制缺陷型腺病毒对前庭和耳蜗毛细胞是安全的,可以作为基因导入的理想载体。
文摘Aim The particle texture from diesel engine was imitated by use of computer. Methods The theory of fractal geometry and the diffusion limited aggregation model were used to simulate the micron texture. Results The fractal dimensions of granule distribution and corpuscle superficial area are quite conformed with those of measurement. Conclusion The texture parameters of engine particle cluster can be obtained precisely by use of fractal theory.
文摘目的比较腺病毒、脂质体、纳米材料三种不同类型的载体体外携带目的基因Math1对HEK293T细胞的转染效率及细胞毒性的大小,以期筛选理想的基因转染载体材料。方法选用重组腺病毒、LipofectamineTM2000、Superfect纳米材料作为转染载体,携带含有增强型绿色荧光蛋白(enhanced green fluorescent protein,EGFP)报告基因的Math1-EGFP基因及真核表达质粒pRK5-Math1-EGFP,并且根据不同转染载体说明步骤优化体外转染条件,分别转染293T细胞。在一定的时间内利用荧光显微镜观察细胞转染结果并计数阳性细胞,采用MTT(噻唑蓝)法检测三种不同载体体外细胞毒性,应用RT-PCR(逆转录聚合酶链反应)技术检测转染阳性细胞中目的基因Math1的mRNA表达情况。结果在优化的体外转染条件下,重组腺病毒载体介导的细胞转染率达到了94%以上,脂质体和商品化Superfect纳米材料转染的细胞中,荧光蛋白表达率分别为73%和80%以上,同时,商品化Superfect纳米材料在达到80%转染率的条件下,细胞存活率为90%。应用RT-PCR方法证实,三种载体转染细胞均有Math1基因的mRNA的表达。结论商品化Superfect纳米材料作为一种新型的、安全有效的纳米转染材料,能够成功携带耳聋治疗关键基因Math1转染293T细胞,以期在内耳基因治疗的研究当中得到有效的应用。
