The process of turning forest area into land is known as deforestation or forest degradation. Reforestation as a fraction of deforestation is extremely low. For improved qualitative and quantitative classification, we...The process of turning forest area into land is known as deforestation or forest degradation. Reforestation as a fraction of deforestation is extremely low. For improved qualitative and quantitative classification, we used Sentinel-1 dataset of State of Para, Brazil to precisely and closely monitor deforestation between June 2019 and June 2023. This research aimed to find out suitable model for classification called Satellite Imaging analysis by Transpose deep neural transformation network (SIT-net) using mathematical model based on Band math approach to classify deforestation applying transpose deep neural network. The main advantage of proposed model is easy to handle SAR images. The study concludes that SAR satellite gives high-resolution images to improve deforestation monitoring and proposed model takes less computational time compared to other techniques.展开更多
目的研究腺病毒携带Math1-EGFP基因经完整圆窗膜途径及鼓阶打孔途径导入耳蜗后对听功能和转导效率的影响,为内耳基因治疗提供实验基础和理论依据。方法健康成年白色红目豚鼠40只,雌雄不限,体重250～300g。随机分成四组,完整圆窗膜组12只...目的研究腺病毒携带Math1-EGFP基因经完整圆窗膜途径及鼓阶打孔途径导入耳蜗后对听功能和转导效率的影响,为内耳基因治疗提供实验基础和理论依据。方法健康成年白色红目豚鼠40只,雌雄不限,体重250～300g。随机分成四组,完整圆窗膜组12只,鼓阶打孔组12只,各组分别设对照8只。实验组(24只)导入重组腺病毒携带的Math1基因及增强型绿色荧光蛋白基因(enhanced green fluorescent protein,EGFP),对照组(16只)导入人工外淋巴液,所有动物均以左耳作为导入耳。术前及术后分别行听性脑干反应(ABR)检查。分别于术后5天、14天取双侧耳蜗标本做基底膜铺片观察基因表达情况。结果完整圆窗膜组导入耳ABR阈值,术后5天各频率与术前比较无显著性差异(P>0.05);鼓阶打孔组导入耳ABR阈值,术后5天在2kHz、4kHz与术前比较无差异(P>0.05),8kHz较术前增高(P<0.05),16kHz、20kHz较术前明显增高(P<0.01),术后14天在16kHz、20kHz较术后5天时明显好转(P<0.01),但较术前仍有增高(P<0.05)。转导成功率鼓阶打孔组为91.6%,优于完整圆窗膜组的50%。两种转导途径对目的基因在耳蜗内的表达部位和表达时间没有显著影响。结论完整圆窗膜途径及鼓阶打孔途径在转导成功率及听功能保护方面各有优劣。完整圆窗膜途径因其对耳蜗的损伤极小,在临床应用方面具有更好的发展前景。展开更多
目的研究以复制缺陷型腺病毒为载体的Math1基因内耳应用的安全性。方法将10只成年Wistar大鼠分为正常对照组和缺失E1、E3基因片段且构建有Math1基因和绿色荧光蛋白报告基因的复制缺陷型腺病毒(adenovirus-Math1-enhanced green fluoresc...目的研究以复制缺陷型腺病毒为载体的Math1基因内耳应用的安全性。方法将10只成年Wistar大鼠分为正常对照组和缺失E1、E3基因片段且构建有Math1基因和绿色荧光蛋白报告基因的复制缺陷型腺病毒(adenovirus-Math1-enhanced green fluorescence protein,Ad-Math1-EGFP)前庭阶导入组(实验组),每组5只,实验组大鼠在右耳通过耳蜗底回前庭阶打孔的方法导入物理滴度为2.1×1011v.p/ml的上述腺病毒5μl,对照组大鼠不做任何处理。7天后对动物进行颈髓硬膜外短声诱发电位(click-evoked potentials on the surface of the cervical dura mater,CDM-CEP)、听性脑干反应(ABR)阈值检测和游泳试验,评价前庭和耳蜗功能,然后将动物处死进行组织形态学观察。结果Ad-Math1-EGFP导入7天后,实验组大鼠前庭及耳蜗的毛细胞及纤毛均未见破坏,形态正常。短声刺激下,对照组大鼠CDM-CEP的阈值为85±3.54dBSPL,ABR阈值为37±4.47dBSPL;实验组大鼠CDM-CEP的阈值为89±6.52dBSPL,ABR阈值为40±3.54dBSPL;对照组大鼠的游泳时间为4.0±0.71s,实验组为5.0±0.71s,两组之间比较差异均无统计学意义。结论携带Math1基因的复制缺陷型腺病毒对前庭和耳蜗毛细胞是安全的,可以作为基因导入的理想载体。展开更多
文摘The process of turning forest area into land is known as deforestation or forest degradation. Reforestation as a fraction of deforestation is extremely low. For improved qualitative and quantitative classification, we used Sentinel-1 dataset of State of Para, Brazil to precisely and closely monitor deforestation between June 2019 and June 2023. This research aimed to find out suitable model for classification called Satellite Imaging analysis by Transpose deep neural transformation network (SIT-net) using mathematical model based on Band math approach to classify deforestation applying transpose deep neural network. The main advantage of proposed model is easy to handle SAR images. The study concludes that SAR satellite gives high-resolution images to improve deforestation monitoring and proposed model takes less computational time compared to other techniques.
文摘目的研究腺病毒携带Math1-EGFP基因经完整圆窗膜途径及鼓阶打孔途径导入耳蜗后对听功能和转导效率的影响,为内耳基因治疗提供实验基础和理论依据。方法健康成年白色红目豚鼠40只,雌雄不限,体重250～300g。随机分成四组,完整圆窗膜组12只,鼓阶打孔组12只,各组分别设对照8只。实验组(24只)导入重组腺病毒携带的Math1基因及增强型绿色荧光蛋白基因(enhanced green fluorescent protein,EGFP),对照组(16只)导入人工外淋巴液,所有动物均以左耳作为导入耳。术前及术后分别行听性脑干反应(ABR)检查。分别于术后5天、14天取双侧耳蜗标本做基底膜铺片观察基因表达情况。结果完整圆窗膜组导入耳ABR阈值,术后5天各频率与术前比较无显著性差异(P>0.05);鼓阶打孔组导入耳ABR阈值,术后5天在2kHz、4kHz与术前比较无差异(P>0.05),8kHz较术前增高(P<0.05),16kHz、20kHz较术前明显增高(P<0.01),术后14天在16kHz、20kHz较术后5天时明显好转(P<0.01),但较术前仍有增高(P<0.05)。转导成功率鼓阶打孔组为91.6%,优于完整圆窗膜组的50%。两种转导途径对目的基因在耳蜗内的表达部位和表达时间没有显著影响。结论完整圆窗膜途径及鼓阶打孔途径在转导成功率及听功能保护方面各有优劣。完整圆窗膜途径因其对耳蜗的损伤极小,在临床应用方面具有更好的发展前景。
文摘目的研究以复制缺陷型腺病毒为载体的Math1基因内耳应用的安全性。方法将10只成年Wistar大鼠分为正常对照组和缺失E1、E3基因片段且构建有Math1基因和绿色荧光蛋白报告基因的复制缺陷型腺病毒(adenovirus-Math1-enhanced green fluorescence protein,Ad-Math1-EGFP)前庭阶导入组(实验组),每组5只,实验组大鼠在右耳通过耳蜗底回前庭阶打孔的方法导入物理滴度为2.1×1011v.p/ml的上述腺病毒5μl,对照组大鼠不做任何处理。7天后对动物进行颈髓硬膜外短声诱发电位(click-evoked potentials on the surface of the cervical dura mater,CDM-CEP)、听性脑干反应(ABR)阈值检测和游泳试验,评价前庭和耳蜗功能,然后将动物处死进行组织形态学观察。结果Ad-Math1-EGFP导入7天后,实验组大鼠前庭及耳蜗的毛细胞及纤毛均未见破坏,形态正常。短声刺激下,对照组大鼠CDM-CEP的阈值为85±3.54dBSPL,ABR阈值为37±4.47dBSPL;实验组大鼠CDM-CEP的阈值为89±6.52dBSPL,ABR阈值为40±3.54dBSPL;对照组大鼠的游泳时间为4.0±0.71s,实验组为5.0±0.71s,两组之间比较差异均无统计学意义。结论携带Math1基因的复制缺陷型腺病毒对前庭和耳蜗毛细胞是安全的,可以作为基因导入的理想载体。
