Neurocircuit regeneration by extracellular matrix reprogramming

The brain's extracellular matrix(ECM),which is comprised of protein and glycosaminoglycan(GAG)scaffolds,constitutes 20%-40% of the human brain and is considered one of the largest influencers on brain cell functioning(Soles et al.,2023).Synthesized by neural and glial cells,the brain's ECM regulates a myriad of homeostatic cellular processes,including neuronal plasticity and firing(Miyata et al.,2012),cation buffering(Moraws ki et al.,2015),and glia-neuron interactions(Anderson et al.,2016).Considering the diversity of functions,dynamic remodeling of the brain's ECM indicates that this understudied medium is an active participant in both normal physiology and neurological diseases.

Stiffness-tunable biomaterials provide a good extracellular matrix environment for axon growth and regeneration

Neuronal growth, extension, branching, and formation of neural networks are markedly influenced by the extracellular matrix—a complex network composed of proteins and carbohydrates secreted by cells. In addition to providing physical support for cells, the extracellular matrix also conveys critical mechanical stiffness cues. During the development of the nervous system, extracellular matrix stiffness plays a central role in guiding neuronal growth, particularly in the context of axonal extension, which is crucial for the formation of neural networks. In neural tissue engineering, manipulation of biomaterial stiffness is a promising strategy to provide a permissive environment for the repair and regeneration of injured nervous tissue. Recent research has fine-tuned synthetic biomaterials to fabricate scaffolds that closely replicate the stiffness profiles observed in the nervous system. In this review, we highlight the molecular mechanisms by which extracellular matrix stiffness regulates axonal growth and regeneration. We highlight the progress made in the development of stiffness-tunable biomaterials to emulate in vivo extracellular matrix environments, with an emphasis on their application in neural repair and regeneration, along with a discussion of the current limitations and future prospects. The exploration and optimization of the stiffness-tunable biomaterials has the potential to markedly advance the development of neural tissue engineering.

THE SPECTRAL RADIUS OF UNIFORM HYPERGRAPH DETERMINED BY THE SIGNLESS LAPLACIAN MATRIX

This paper studies the problem of the spectral radius of the uniform hypergraph determined by the signless Laplacian matrix.The upper bound of the spectral radius of a uniform hypergraph is obtained by using Rayleigh principle and the perturbation of the spectral radius under moving the edge operation,and the extremal hypergraphs are characterized for both supertree and unicyclic hypergraphs.The spectral radius of the graph is generalized.

A matrix metalloproteinase-responsive hydrogel system controls angiogenic peptide release for repair of cerebral ischemia/reperfusion injury

Vascular endothelial growth factor and its mimic peptide KLTWQELYQLKYKGI(QK)are widely used as the most potent angiogenic factors for the treatment of multiple ischemic diseases.However,conventional topical drug delivery often results in a burst release of the drug,leading to transient retention(inefficacy)and undesirable diffusion(toxicity)in vivo.Therefore,a drug delivery system that responds to changes in the microenvironment of tissue regeneration and controls vascular endothelial growth factor release is crucial to improve the treatment of ischemic stroke.Matrix metalloproteinase-2(MMP-2)is gradually upregulated after cerebral ischemia.Herein,vascular endothelial growth factor mimic peptide QK was self-assembled with MMP-2-cleaved peptide PLGLAG(TIMP)and customizable peptide amphiphilic(PA)molecules to construct nanofiber hydrogel PA-TIMP-QK.PA-TIMP-QK was found to control the delivery of QK by MMP-2 upregulation after cerebral ischemia/reperfusion and had a similar biological activity with vascular endothelial growth factor in vitro.The results indicated that PA-TIMP-QK promoted neuronal survival,restored local blood circulation,reduced blood-brain barrier permeability,and restored motor function.These findings suggest that the self-assembling nanofiber hydrogel PA-TIMP-QK may provide an intelligent drug delivery system that responds to the microenvironment and promotes regeneration and repair after cerebral ischemia/reperfusion injury.

Immunohistochemical expression of matrix metalloproteinase-9 and 13 in oral squamous cell carcinoma and their role in predicting lymph node metastasis

BACKGROUND One of the main characteristics of oral squamous cell carcinoma(OSCC)is that it metastasizes to cervical lymph nodes frequently with a high degree of local invasiveness.A primary feature of malignant tumors is their penetration of neighboring tissues,such as lymphatic and blood arteries,due to the tumor cells'capacity to break down the extracellular matrix(ECM).Matrix metalloproteinases(MMPs)constitute a family of proteolytic enzymes that facilitate tissue remo-deling and the degradation of the ECM.MMP-9 and MMP-13 belong to the group of extracellular matrix degrading enzymes and their expression has been studied in OSCC because of their specific functions.MMP-13,a collagenase family member,is thought to play an essential role in the MMP activation cascade by breaking down the fibrillar collagens,whereas MMP-9 is thought to accelerate the growth of tumors.Elevated MMP-13 expression has been associated with tumor behavior and patient prognosis in a number of malignant cases.The authors wish to thank Jadhav KB for his valuable opinion during the preparation of the manuscript.

基于二阶统计特性的方向向量估计算法的DOA估计

为了减小天线阵流形误差对波达方向(DOA)估计结果的影响,以及克服基于传统盲源分离算法的DOA估计算法不能应用于少通道测向设备的不足,提出一种基于2阶统计特性的方向向量估计算法的DOA估计算法。首先,根据确定性最大似然(DML)估计算法谱函数的特征,构造关于协方差矩阵的酉约束下的优化问题;然后,通过优化该问题获得各个单信号的实际方向向量;最后,将各个单信号的实际方向向量输入到空间谱算法中实现DOA估计。由于将多信号的DOA估计转化为多个单信号的DOA估计,因此在天线阵列流形存在误差时,所提算法比传统的DOA方法具有更好的DOA估计性能。由于所提算法仅需使用协方差矩阵,因此所提算法可应用于少通道测向设备。由仿真实验结果可知,在阵列流形存在误差以及测向设备为少通道测向设备时,与传统DOA方法相比,所提算法的DOA估计的准确度、抗扰度以及分辨率更高。

阵元失效下稀疏阵列的二维DOA估计算法

本文针对二维稀疏阵列在阵元失效条件下,因数据缺失导致虚拟阵列连续性被破坏及自由度下降的问题,提出了一种二维DOA估计算法。首先基于二维差分共阵构建虚拟阵列,然后利用解耦原子范数最小化理论,以矩阵填充的形式恢复协方差矩阵数据,实现对虚拟阵列中丢失虚拟阵元的内插,最后采用SS-MUSIC算法进行多信源的二维DOA估计。所提方法弥补了物理阵元失效所造成的影响,恢复了原始虚拟阵列的完整孔径特性,保持了虚拟阵列的自由度,从而确保了较高精度的二维DOA估计性能。仿真实验结果表明,在相同阵元数量及阵元失效情况下,本文提出的算法相比已有方法能有效地估计更多信源,并在小快拍数和低信噪比条件下表现出更高的稳健性,最大限度地保留并利用了稀疏阵列在二维DOA估计中的自由度优势。

基于复合阵列的矩阵循环重构DOA估计算法

相干源角度估计一直是困扰雷达角度测量的一个重要难题,基于均匀阵列假设提出了模式空间解相干算法,一定程度上解决了相干干扰条件下的目标角度估计问题,但是上述算法对复合阵列的适应性较差。针对复合阵列下相干多源测角问题,本文提出一种基于模式空间循环重构的解相关算法,利用圆阵外其他任意天线赋形空域滤波特性,在信号源数多于阵元数时较好地解决相干源角度估计问题。仿真试验结果表明,该算法能较好地解决复合阵列天线下的相干源测角问题,并且其角分辨性能优于传统算法。

一种基于稀疏贝叶斯学习的离网DOA估计算法

本文提出一种基于稀疏贝叶斯学习的改进离网DOA估计算法,以提升非理想测向环境下在低信噪比、低快拍数时的DOA估计性能,称之为MOGSBL算法。本算法将信号源方位区间进行离散化,得到方位离散网格。为阵列接收信号建立稀疏贝叶斯模型,将网格节点修正量设为模型超参数。采用期望最大化算法迭代更新网格节点修正量,使更新后的网格节点更接近真实源信号方位。为了检验MOGSBL算法的性能,本文进行了大量的数值实验,并将MOGSBL算法的DOA估计结果与RSBL算法、OGSBL算法和L1-SVD算法进行对比。在不同信噪比和不同快拍数时,MOGSBL算法均能清晰分辨方位很接近的两个信号源,角度分辨率明显高于RSBL算法、OGSBL算法和L1-SVD算法。随着信噪比和快拍数的增加,4种算法的RMSE均逐渐减小。但MOGSBL算法的RMSE明显低于RSBL算法、OGSBL算法和L1-SVD算法,且RSBL算法、OGSBL算法优于L1-SVD算法。实验还分析了方向测试范围的离散网格节点数对DOA估计的影响,发现细密的离散网格可以提高DOA估计精度,但DOA估计的计算量会增加。且在任意网格节点数时,相比于RSBL算法、OGSBL算法和L1-SVD算法,本文的MOGSBL算法均具有最低的RMSE和最短的计算时间。

基于低秩块Hankel矩阵正则化的阵元故障MIMO雷达DOA估计

多输入多输出(Multiple-Input Multiple-Output,MIMO)雷达在阵元故障时虚拟阵列输出数据矩阵会出现大量的整行数据丢失,由于阵列接收数据矩阵的不完整而导致对波达方向(Direction of Arrival,DOA)的估计性能恶化。大多数低秩矩阵填充算法要求缺失数据随机分布于不完整的矩阵中,无法适用于整行缺失数据的恢复问题。为此,提出了一种基于低秩块Hankel矩阵正则化的阵元故障MIMO雷达DOA估计方法。首先,通过奇异值分解(Singular Value Decomposition,SVD)降低虚拟阵列输出矩阵的维度,以减少计算复杂度。然后,对降维数据矩阵建立基于块Hankel矩阵正则化的低秩矩阵填充模型,在该模型中将MIMO雷达降维数据矩阵排列成块Hankel矩阵并施加Schatten-p范数作为正则项。最后,结合交替方向乘子法(Alternate Direction Multiplier Method,ADMM)求解该模型,获得完整的MIMO雷达降维数据矩阵。仿真结果表明,所提方法能够有效恢复降维数据矩阵中的整行数据缺失,具有较高的DOA估计精度和实时性,在阵元故障率低于50.0%时DOA估计精度优于现有方法。

大间距均匀阵列抗模糊DOA估计实验研究

在波达方向(DOA)估计中,当接收天线阵列的单元间距大于入射信号载波的半波长时,会出现阵列流形模糊,这对分辨真实的信号DOA带来了挑战。该文基于子阵级大间距均匀双线极化阵列,提出通过随机改变每个子阵中某个单元的极化状态,使不同子阵对应的导向矢量相互独立,从而打破大间距均匀阵列下导向矢量间的线性关系,并抑制阵列流形模糊。仿真和实验结果表明,该方法可有效去除多重信号分类(MUSIC)算法空间谱中的伪峰,实现高精度DOA估计。

一种基于接收数据重构的DOA估计技术

在传统测向方法中,测向精度正比于阵列孔径,因此布阵空间与测向精度的矛盾性成为电子侦察系统在无人机等平台应用的主要工程约束之一。为实现空间受限下的高精度测向,提出一种通过协方差矩阵重构阵列接收数据的波达方向(Direction of Arrival,DOA)方法。结合均匀线阵的结构特点以及导向矢量Vandermonde矩阵与协方差Toeplitz矩阵的矩阵特征,通过重构阵列数据接收模型,实现阵列孔径的拓展,可在布阵空间不变的条件下显著提升阵列的测向精度。仿真结果表明,这种基于协方差数据重构的DOA方法实用有效,可作为传统DOA技术的前处理手段,提升算法性能及处理增益。

范数在稀疏重构类DOA估计领域的应用

本文主要探讨了基于稀疏重构理论的DOA估计方法,以及利用范数进行稀疏性约束的优化方法.

基于稀疏度自适应变步长的离格DOA估计方法

网格划分产生的量化误差是影响信源定位估计性能的一个重要缺陷。针对目前Lp类离格算法计算量大以及需要提前预知稀疏度的问题,提出了一种稀疏度自适应变步长的离格波达方向定位方法。首先根据一阶泰勒展开构建基于角度优化的离格参数模型,以残差能量的变化作为预估稀疏度K的条件。然后利用噪声子空间与信号子空间正交性作为原子误差入选判定依据,利用交替迭代优化方法实现离格模型下的准确求解。所提方法结合了贪婪算法支撑集选取策略与阵列协方差矩阵的有效信息。仿真实验表明,在满足稀疏性条件下,所提方法不仅大大缩短运算时间,而且可以实现空域角度范围内任意角度的精确估计。

一种协同二维DOA和TDOA观测量的超视距短波辐射源定位新方法

针对超视距远距离短波辐射源定位误差较大的问题,该文在观测站同时获得二维到达角度和到达时间差参数的场景下,提出一种协同这两种观测量的定位新方法。首先,基于单跳电离层虚高模型构建面向短波辐射源的二维到达角度和到达时间差的非线性观测方程。然后,将超视距定位几何模型与代数模型相结合,并依次将两种非线性观测方程转化为伪线性观测方程,进而提出一种无需迭代的两阶段协同定位方法。阶段1通过求解一元六次多项式的根获得目标位置向量闭式解,阶段2通过构建等式约束优化模型对阶段1的估计误差进行改良,并利用拉格朗日乘子技术得到精度更高的定位结果。最后,利用约束误差扰动理论对新提出的协同定位方法的估计性能进行理论分析,证明新方法具有渐近统计最优性,同时还利用约束误差扰动理论定量分析短波辐射源高度信息误差对定位精度产生的影响,并推导能确保地球椭圆约束产生性能增益的短波辐射源高度信息误差最大门限值。仿真实验结果验证该文新方法能够获得显著的协同增益。

基于稀疏恢复的快速高精度DOA估计算法

传统的基于稀疏恢复的波达方向(direction of arrival,DOA)估计算法使用密集的采样网格,导致计算量显著增加,且对邻近入射信号的估计精度不高。针对这一问题,提出一种快速高精度DOA估计算法。该算法首先使用网格进化方法降低网格点总数。然后,对噪声方差和信号功率进行二次估计,进而使用离网求根稀疏贝叶斯学习(off-grid root sparse Bayesian learning,OGRSBL)技术来实现入射角的精确估计。仿真表明,相比传统稀疏贝叶斯学习类算法,所提算法计算效率高,同时对紧邻信号有着更好的估计能力。

DOA estimation of coexisted noncoherent and coherent signals via sparse representation of cleaned array covariance matrix

A new direction finding method is presented to deal with coexisted noncoherent and co- herent signals without smoothing operation. First the direction-of-arrival （DOA） estimation task is herein reformulated as a sparse reconstruction problem of the cleaned array covariance matrix, which is processed to eliminate the affection of the noise. Then by using the block of matrices, the information of DOAs which we pursuit are implied in the sparse coefficient matrix. Finally, the sparse reconstruction problem is solved by the improved M-FOCUSS method, which is applied to the situation of block of matrices. This method outperforms its data domain counterpart in terms of noise suppression, and has a better performance in DOA estimation than the customary spatial smoothing technique. Simulation results verify the efficacy of the proposed method.

Construction of deterministic sensing matrix and its application to DOA estimation

Compressive sensing（CS） has emerged as a novel sampling framework which enables sparse signal acquisition and reconstruction with fewer measurements below the Nyquist rate.An important issue for CS is the construction of measurement matrix or sensing matrix.A new deterministic sensing matrix,named as OOC-B,is proposed by exploiting optical orthogonal codes（OOCs）,Bernoulli matrix and Singer structure,which has the entries of 0,＋1 and-1 before normalization.We have proven that the designed deterministic matrix is asymptotically optimal.In addition,the proposed deterministic sensing matrix is applied to direction of arrival（DOA） estimation of narrowband signals by CS arrays（CSA）processing and CS recovery.Theoretical analysis and simulation results show that the proposed sensing matrix has good performance for DOA estimation.It is very effective for simplifying hardware structure and decreasing computational complexity in DOA estimation by CSA processing.Besides,lower root mean square error（RMSE） and bias are obtained in DOA estimation by CS recovery.

基于稀疏线阵协方差矩阵重构的DOA估计方法研究

相比均匀线阵(Uniform Linear Array,ULA),相同阵元数目下稀疏线阵(Sparse Linear Array,SLA)的抗耦合效应更好,阵列孔径更大,到达方向(Direction of Arrival,DOA)估计的自由度(Degrees Of Freedom,DOF)更高,因而近年来得到了广泛的研究。为了可以进行高DOF的DOA估计,学者们开始研究SLA的差分虚拟阵元,差分虚拟阵元对应的协方差矩阵相比原阵元对应的协方差矩阵维度更大,因而估计的DOF更高。当SLA的差分虚拟阵元连续取值时,可以利用已有阵元的接收信息,得到SLA的协方差矩阵,在该矩阵的基础之上构建差分虚拟阵元的协方差矩阵进而进行DOA估计。然而,当SLA的差分虚拟阵元存在孔洞时,即差分虚拟阵元不能连续取值时,不能直接利用重构的协方差矩阵进行DOA估计,需要恢复完全增广协方差矩阵的信息再进行DOA估计。对于该问题,本文基于矢量化后原协方差矩阵和虚拟差分阵协方差矩阵的误差分布情况,并结合完全增广协方差矩阵的低秩特性和半正定特性来构建优化问题。通过求解该问题来恢复维度更高的完全增广协方差矩阵。最后对该矩阵进行奇异值分解,利用多重信号分类(Multiple Signal Classification,MUSIC)算法就可以获得多源的空间谱。本文最后通过数值仿真试验验证了所提算法可以实现高DOF的DOA估计,并且相比于现有算法,本文所提算法对欠定DOA估计的效果更好,多源DOA估计的精度更高,产生的误差更小。

稀疏阵列的鲁棒矩阵填充DOA估计算法

稀疏阵列布阵灵活,增大阵列孔径的同时还能减少阵元间耦合,但基于稀疏阵列的传统波达方向估计会导致角度模糊混叠,带来估计精度差和稳健性不足的问题。针对以上问题,提出一种适用于稀疏阵列波达方向估计的加权截断奇异值投影(weighted truncated singular value projection,WT-SVP)的鲁棒矩阵填充算法。在填充迭代过程中根据奇异值的大小分配权重,突出大奇异值包含的阵列信息,减少小奇异值中不必要的噪声信息,从而优化传统奇异值投影算法。该算法可以实现稀疏阵列的孔洞信息恢复,对不连续阵元充分利用,同时WT-SVP填充算法实现了稀疏阵列波达方向估计的高精度、高分辨以及在低信噪比、低快拍时的高鲁棒性。