This study investigated the bioremediation of organic pollution in soil and water systems by cyanobacterial mats collected from Wadi Gaza. Acetochlor, a model compound of herbicide, was used as a standard organic poll...This study investigated the bioremediation of organic pollution in soil and water systems by cyanobacterial mats collected from Wadi Gaza. Acetochlor, a model compound of herbicide, was used as a standard organic pollutant. Various concentrations of acetochlor were injected in soil and water samples pre-treated with cyanobacterial mat for several periods of time. Percentage of growth of wheat as a test plant was taken as indicator of bioremediation of acetochlor. Results showed that acetochlor was degraded in both soil and water systems. Degradation was?much?faster in the water system than in the soil system. Concentrations of acetochlor above the field rate did not affect the bioremediation process in the water system whereas it did in soil pots. Furthermore, bioremediation in water system was nearly completed in 15 days whereas it did not reach high percentage?in the soil system. These encouraging results are new contribution in field of bioremediation of pesticide by cyanobacterial mats and suggest that application of cyanobacterial mat could be a fast and suitable methodology for bioremediation of organic pollutant in the ecosystem.展开更多
Lightweight roof greening is an important way for improving urban ecological environment and has good ecological and social benefits, but the investment is- too-high for the investors. Therefore, it is necessary to im...Lightweight roof greening is an important way for improving urban ecological environment and has good ecological and social benefits, but the investment is- too-high for the investors. Therefore, it is necessary to improve the system of lightweight roof greening. This study introduced a lightweight roof greening mode with low cost, simple construction, rapid formation, good economic benefit and convenient curing.展开更多
A new matting algorithm based on color distance and differential distance is proposed to deal with the problem that many matting methods perform poorly with complex natural images.The proposed method combines local sa...A new matting algorithm based on color distance and differential distance is proposed to deal with the problem that many matting methods perform poorly with complex natural images.The proposed method combines local sampling with global sampling to select foreground and background pairs for unknown pixels and then a new cost function is constructed based on color distance and differential distance to further optimize the selected sample pairs.Finally,a quadratic objective function is used based on matte Laplacian coming from KNN matting which is added with texture feature.Through experiments on various test images,it is confirmed that the results obtained by the proposed method are more accurate than those obtained by traditional methods.The four-error-metrics comparison on benchmark dataset among several algorithms also proves the effectiveness of the proposed method.展开更多
Background:The local pivotal method(LPM)utilizing auxiliary data in sample selection has recently been proposed as a sampling method for national forest inventories(NFIs).Its performance compared to simple random samp...Background:The local pivotal method(LPM)utilizing auxiliary data in sample selection has recently been proposed as a sampling method for national forest inventories(NFIs).Its performance compared to simple random sampling(SRS)and LPM with geographical coordinates has produced promising results in simulation studies.In this simulation study we compared all these sampling methods to systematic sampling.The LPM samples were selected solely using the coordinates(LPMxy)or,in addition to that,auxiliary remote sensing-based forest variables(RS variables).We utilized field measurement data(NFI-field)and Multi-Source NFI(MS-NFI)maps as target data,and independent MS-NFI maps as auxiliary data.The designs were compared using relative efficiency(RE);a ratio of mean squared errors of the reference sampling design against the studied design.Applying a method in NFI also requires a proven estimator for the variance.Therefore,three different variance estimators were evaluated against the empirical variance of replications:1)an estimator corresponding to SRS;2)a Grafström-Schelin estimator repurposed for LPM;and 3)a Matérn estimator applied in the Finnish NFI for systematic sampling design.Results:The LPMxy was nearly comparable with the systematic design for the most target variables.The REs of the LPM designs utilizing auxiliary data compared to the systematic design varied between 0.74–1.18,according to the studied target variable.The SRS estimator for variance was expectedly the most biased and conservative estimator.Similarly,the Grafström-Schelin estimator gave overestimates in the case of LPMxy.When the RS variables were utilized as auxiliary data,the Grafström-Schelin estimates tended to underestimate the empirical variance.In systematic sampling the Matérn and Grafström-Schelin estimators performed for practical purposes equally.Conclusions:LPM optimized for a specific variable tended to be more efficient than systematic sampling,but all of the considered LPM designs were less efficient than the systematic sampling design for some target variables.The Grafström-Schelin estimator could be used as such with LPMxy or instead of the Matérn estimator in systematic sampling.Further studies of the variance estimators are needed if other auxiliary variables are to be used in LPM.展开更多
At present time, there has been a demand for management systems that can survey and monitor a PC （personal computer） practice room, movement of people in an office, situation of utilization of facilities and so on i...At present time, there has been a demand for management systems that can survey and monitor a PC （personal computer） practice room, movement of people in an office, situation of utilization of facilities and so on in real time without causing psychological stress. For example, PC administrators in Japanese national educational institutions must report operation status of PC practice rooms once a year. But, there is currently no system for automatically recording PC operating situations. Therefore, the burden on the PC administrators is big. In this study, we aimed at systems for accurately managing the sitting and work time without psychologically stressing PC users. This time, we propose uniform management systems of sitting and work time using smart tap node and mat sensor node by IoT （Internet of Things） technology. The smart tap was connected to PCs to acquire the operating status of the PCs. In parallel with this smart tap, the mat sensor was used to acquire human presence state. By calculating binary data representing the PC operating status and human presence state from the two sensing data （smart tap and mat sensor） by the proposed technique, we can exactly calculate wasteful power consumption etc. The use of IoT technology makes it unnecessary to use large installation services when introducing our system. Therefore, this our proposal system can be easily installed even by unskilled workers.展开更多
Bayesian Matting has four limitations.Firstly,Bayesian matting makes strong assumption that the texture distribution of nature image satisfies Gaussian distribution with fixed variance.This assumption will fail for co...Bayesian Matting has four limitations.Firstly,Bayesian matting makes strong assumption that the texture distribution of nature image satisfies Gaussian distribution with fixed variance.This assumption will fail for complex texture distribution.In order to extract the nature images with complex texture distribution,we design an information entropy approach to estimate the scalable variance.Secondly,when the opacity is near the boundary of the value range,Bayesian matting method may be failure because of the error computation of opacity.Therefore,a rectification approach is proposed to adjust the computation model and keep the opacity within the valid value range.Thirdly,Bayesian matting is a local sample method which may miss some valid samples of matting.We propose a selection function to integrate valid global sample matting result into above matting framework as a supplement to the local sample matting result.The proposed function is compose of three criteria,that is,the similarity of results,the overlapping degree of samples,and the similarity of neighborhood.Fourthly,in order to obtain a smooth and vivid matte,the result is further refined by considering correlation between neighbouring pixels.Finally,We use online benchmark for image matting to evaluate the proposed method with both qualitative observation and quantitative analysis.The experiments show that our method achieves a competitive advantages over other methods.展开更多
To investigate the existence of the major outer membrane protein (MOMP) gene LipL32 in 15 dominant Chinese strains of 15 serogroups of Leptospira interrogans and 2 international strains of 2 serogroups of Leptospira b...To investigate the existence of the major outer membrane protein (MOMP) gene LipL32 in 15 dominant Chinese strains of 15 serogroups of Leptospira interrogans and 2 international strains of 2 serogroups of Leptospira biflexa, and to clone and construct the expression system as well as to identify the recombinant proteins, genomic DNAs from strains of leptospira were prepared by routine phenol-chloroform method, and the fragments of the LipL32 gene with the whole length from the strains were amplified with high fidelity PCR. The target amplification products were sequenced after T-A cloning, and the expression system for the genes were thereby constructed. Expression of the recombinant proteins was identified by using SDS-PAGE after induction with IPTG at different dosages. Western blot assays with rabbit antiserum against the whole cell of TR/PatocⅠ of Leptospira and immunized serum with rMOMPs were used to determine the immunoreactivity and immunogenicity of the recombinant proteins. Microscopic agglutination test was used to determine the cross- agglutination titres in rabbit sera immunized with rMOMPs, and the cell adherence model of Leptospira was used to examine the blocking effects of rabbit antisera against these rMOMPs. It was found that the LipL32 gene could be found in all the 17 strains of Leptospira mentioned above with two different genotypes, i.e. LipL32/1 and LipL32/2. Amounts of expressions of rMOMP1 and rMOMP2 after IPTG accounted for 40% and 10% of the total bacterial proteins respectively. Both rMOMP1 and rMOMP2 could combine with the rabbit antiserum against leptospiral TR/PatocⅠ, and could induce the production of agglutination antibodies to these 17 strains of Leptospira with 1∶2 to 1∶64 MAT titres. The rabbit anti-rMOMP1 and anti-MOMP2 antibodies at 1∶2 to 1∶16 dilutions could efficiently block adherence of Leptospira. It concludes that all the Leptospira tested in the present study possess LipL32/1 or LipL32/2 genes, and the constructed expression system can express the rMOMP1 and rMOMP2. These recombinant proteins are showed to have good immunogenicity and satisfactory immunoreactivity.展开更多
While the geodetic excitationχ(t)of polar motion p(t)is essential to improve our understanding of global mass redistributions and relative motions with respect to the terrestrial frame,the widely adopted method to de...While the geodetic excitationχ(t)of polar motion p(t)is essential to improve our understanding of global mass redistributions and relative motions with respect to the terrestrial frame,the widely adopted method to deriveχ(t)from p(t)has biases in both amplitude and phase responses.This study has developed a new simple but more accurate method based on the combination of the frequency-and time-domain Liouville's equation(FTLE).The FTLE method has been validated not only with 6-h sampled synthetic excitation series but also with daily and 6-h sampled polar motion measurements as well asχ(t)produced by the interactive webpage tool of the International Earth Rotation and Reference Systems Service(IERS).Numerical comparisons demonstrate thatχ(t)derived from the FTLE method has superior performances in both the time and frequency domains with respect to that obtained from the widely adopted method or the IERS webpage tool,provided that the input p(t)series has a length around or more than 25 years,which presents no practical limitations since the necessary polar motion data are readily available.The FTLE code is provided in the form of Mat Lab function.展开更多
Interference management is one of the most important issues in the device-to-device(D2D)-enabled heterogeneous cellular networks(HetCNets)due to the coexistence of massive cellular and D2D devices in which D2D devices...Interference management is one of the most important issues in the device-to-device(D2D)-enabled heterogeneous cellular networks(HetCNets)due to the coexistence of massive cellular and D2D devices in which D2D devices reuse the cellular spectrum.To alleviate the interference,an efficient interference management way is to set exclusion zones around the cellular receivers.In this paper,we adopt a stochastic geometry approach to analyze the outage probabilities of cellular and D2D users in the D2D-enabled HetCNets.The main difficulties contain three aspects:1)how to model the location randomness of base stations,cellular and D2D users in practical networks;2)how to capture the randomness and interrelation of cellular and D2D transmissions due to the existence of random exclusion zones;3)how to characterize the different types of interference and their impacts on the outage probabilities of cellular and D2D users.We then run extensive Monte-Carlo simulations which manifest that our theoretical model is very accurate.展开更多
BACKGROUND Kinesiophobia is a common condition often manifested in patients with musculoskeletal disorders within the process of rehabilitation.Recently,the literature has been investigating whether Pilates could cont...BACKGROUND Kinesiophobia is a common condition often manifested in patients with musculoskeletal disorders within the process of rehabilitation.Recently,the literature has been investigating whether Pilates could contribute to the management of kinesiophobia in various musculoskeletal disorders.However,aggregated data regarding its effectiveness are absent from literature.AIM To evaluate recordings of the Pilates method in kinesiophobia related to musculoskeletal disorders.METHODS PubMed,ScienceDirect,Scopus and Pedro databases were all scrutinized for randomized controlled trials,by two or more intervention groups,where at least one group received a Pilates-based intervention and which had been conducted in patients aged 18-65 years with musculoskeletal disorders,having assessed at least one outcome related to kinesiophobia.The systematic review was based on the PRISMA guidelines.RESULTS We have identified five studies,with a total of 366 patients with musculoskeletal disorders.Three of them showed that a Pilates-based intervention by either mat or equipment can combat kinesiophobia in patients with musculoskeletal conditions,while another showed that Pilates exercises with equipment may have better long-term effects on kinesiophobia compared to Pilates mat.CONCLUSION Overall,a strong level of research evidence has been amassed for the Pilates intervention as well as a moderate level of research evidence for the effectiveness of equipment-based Pilates in reducing kinesiophobia in patients with musculoskeletal disorders.While the underlying mechanisms driving such a result remain unknown,it appears that Pilates can influence both biological and psychological factors in musculoskeletal disorders,thus resulting in the management of kinesiophobic behaviours.展开更多
文摘This study investigated the bioremediation of organic pollution in soil and water systems by cyanobacterial mats collected from Wadi Gaza. Acetochlor, a model compound of herbicide, was used as a standard organic pollutant. Various concentrations of acetochlor were injected in soil and water samples pre-treated with cyanobacterial mat for several periods of time. Percentage of growth of wheat as a test plant was taken as indicator of bioremediation of acetochlor. Results showed that acetochlor was degraded in both soil and water systems. Degradation was?much?faster in the water system than in the soil system. Concentrations of acetochlor above the field rate did not affect the bioremediation process in the water system whereas it did in soil pots. Furthermore, bioremediation in water system was nearly completed in 15 days whereas it did not reach high percentage?in the soil system. These encouraging results are new contribution in field of bioremediation of pesticide by cyanobacterial mats and suggest that application of cyanobacterial mat could be a fast and suitable methodology for bioremediation of organic pollutant in the ecosystem.
基金Supported by Science and Technology Planning Project of Guangdong Province,China(No.2015B090904008)Soft Science Planning Project of Guangdong Province(2014B090903015)Ecological Environment Construction and Protection(Techand)Engineering and Technological Research Center(YKHZZ[2013]1589)~~
文摘Lightweight roof greening is an important way for improving urban ecological environment and has good ecological and social benefits, but the investment is- too-high for the investors. Therefore, it is necessary to improve the system of lightweight roof greening. This study introduced a lightweight roof greening mode with low cost, simple construction, rapid formation, good economic benefit and convenient curing.
基金Supported by the National Natural Science Foundation of China(No.61133009,U1304616)
文摘A new matting algorithm based on color distance and differential distance is proposed to deal with the problem that many matting methods perform poorly with complex natural images.The proposed method combines local sampling with global sampling to select foreground and background pairs for unknown pixels and then a new cost function is constructed based on color distance and differential distance to further optimize the selected sample pairs.Finally,a quadratic objective function is used based on matte Laplacian coming from KNN matting which is added with texture feature.Through experiments on various test images,it is confirmed that the results obtained by the proposed method are more accurate than those obtained by traditional methods.The four-error-metrics comparison on benchmark dataset among several algorithms also proves the effectiveness of the proposed method.
基金the Ministry of Agriculture and Forestry key project“Puuta liikkeelle ja uusia tuotteita metsästä”(“Wood on the move and new products from forest”)Academy of Finland(project numbers 295100 , 306875).
文摘Background:The local pivotal method(LPM)utilizing auxiliary data in sample selection has recently been proposed as a sampling method for national forest inventories(NFIs).Its performance compared to simple random sampling(SRS)and LPM with geographical coordinates has produced promising results in simulation studies.In this simulation study we compared all these sampling methods to systematic sampling.The LPM samples were selected solely using the coordinates(LPMxy)or,in addition to that,auxiliary remote sensing-based forest variables(RS variables).We utilized field measurement data(NFI-field)and Multi-Source NFI(MS-NFI)maps as target data,and independent MS-NFI maps as auxiliary data.The designs were compared using relative efficiency(RE);a ratio of mean squared errors of the reference sampling design against the studied design.Applying a method in NFI also requires a proven estimator for the variance.Therefore,three different variance estimators were evaluated against the empirical variance of replications:1)an estimator corresponding to SRS;2)a Grafström-Schelin estimator repurposed for LPM;and 3)a Matérn estimator applied in the Finnish NFI for systematic sampling design.Results:The LPMxy was nearly comparable with the systematic design for the most target variables.The REs of the LPM designs utilizing auxiliary data compared to the systematic design varied between 0.74–1.18,according to the studied target variable.The SRS estimator for variance was expectedly the most biased and conservative estimator.Similarly,the Grafström-Schelin estimator gave overestimates in the case of LPMxy.When the RS variables were utilized as auxiliary data,the Grafström-Schelin estimates tended to underestimate the empirical variance.In systematic sampling the Matérn and Grafström-Schelin estimators performed for practical purposes equally.Conclusions:LPM optimized for a specific variable tended to be more efficient than systematic sampling,but all of the considered LPM designs were less efficient than the systematic sampling design for some target variables.The Grafström-Schelin estimator could be used as such with LPMxy or instead of the Matérn estimator in systematic sampling.Further studies of the variance estimators are needed if other auxiliary variables are to be used in LPM.
文摘At present time, there has been a demand for management systems that can survey and monitor a PC （personal computer） practice room, movement of people in an office, situation of utilization of facilities and so on in real time without causing psychological stress. For example, PC administrators in Japanese national educational institutions must report operation status of PC practice rooms once a year. But, there is currently no system for automatically recording PC operating situations. Therefore, the burden on the PC administrators is big. In this study, we aimed at systems for accurately managing the sitting and work time without psychologically stressing PC users. This time, we propose uniform management systems of sitting and work time using smart tap node and mat sensor node by IoT （Internet of Things） technology. The smart tap was connected to PCs to acquire the operating status of the PCs. In parallel with this smart tap, the mat sensor was used to acquire human presence state. By calculating binary data representing the PC operating status and human presence state from the two sensing data （smart tap and mat sensor） by the proposed technique, we can exactly calculate wasteful power consumption etc. The use of IoT technology makes it unnecessary to use large installation services when introducing our system. Therefore, this our proposal system can be easily installed even by unskilled workers.
文摘Bayesian Matting has four limitations.Firstly,Bayesian matting makes strong assumption that the texture distribution of nature image satisfies Gaussian distribution with fixed variance.This assumption will fail for complex texture distribution.In order to extract the nature images with complex texture distribution,we design an information entropy approach to estimate the scalable variance.Secondly,when the opacity is near the boundary of the value range,Bayesian matting method may be failure because of the error computation of opacity.Therefore,a rectification approach is proposed to adjust the computation model and keep the opacity within the valid value range.Thirdly,Bayesian matting is a local sample method which may miss some valid samples of matting.We propose a selection function to integrate valid global sample matting result into above matting framework as a supplement to the local sample matting result.The proposed function is compose of three criteria,that is,the similarity of results,the overlapping degree of samples,and the similarity of neighborhood.Fourthly,in order to obtain a smooth and vivid matte,the result is further refined by considering correlation between neighbouring pixels.Finally,We use online benchmark for image matting to evaluate the proposed method with both qualitative observation and quantitative analysis.The experiments show that our method achieves a competitive advantages over other methods.
文摘To investigate the existence of the major outer membrane protein (MOMP) gene LipL32 in 15 dominant Chinese strains of 15 serogroups of Leptospira interrogans and 2 international strains of 2 serogroups of Leptospira biflexa, and to clone and construct the expression system as well as to identify the recombinant proteins, genomic DNAs from strains of leptospira were prepared by routine phenol-chloroform method, and the fragments of the LipL32 gene with the whole length from the strains were amplified with high fidelity PCR. The target amplification products were sequenced after T-A cloning, and the expression system for the genes were thereby constructed. Expression of the recombinant proteins was identified by using SDS-PAGE after induction with IPTG at different dosages. Western blot assays with rabbit antiserum against the whole cell of TR/PatocⅠ of Leptospira and immunized serum with rMOMPs were used to determine the immunoreactivity and immunogenicity of the recombinant proteins. Microscopic agglutination test was used to determine the cross- agglutination titres in rabbit sera immunized with rMOMPs, and the cell adherence model of Leptospira was used to examine the blocking effects of rabbit antisera against these rMOMPs. It was found that the LipL32 gene could be found in all the 17 strains of Leptospira mentioned above with two different genotypes, i.e. LipL32/1 and LipL32/2. Amounts of expressions of rMOMP1 and rMOMP2 after IPTG accounted for 40% and 10% of the total bacterial proteins respectively. Both rMOMP1 and rMOMP2 could combine with the rabbit antiserum against leptospiral TR/PatocⅠ, and could induce the production of agglutination antibodies to these 17 strains of Leptospira with 1∶2 to 1∶64 MAT titres. The rabbit anti-rMOMP1 and anti-MOMP2 antibodies at 1∶2 to 1∶16 dilutions could efficiently block adherence of Leptospira. It concludes that all the Leptospira tested in the present study possess LipL32/1 or LipL32/2 genes, and the constructed expression system can express the rMOMP1 and rMOMP2. These recombinant proteins are showed to have good immunogenicity and satisfactory immunoreactivity.
基金supported by the National Natural Science Foundation of China(grant numbers 41874025 and 41474022)。
文摘While the geodetic excitationχ(t)of polar motion p(t)is essential to improve our understanding of global mass redistributions and relative motions with respect to the terrestrial frame,the widely adopted method to deriveχ(t)from p(t)has biases in both amplitude and phase responses.This study has developed a new simple but more accurate method based on the combination of the frequency-and time-domain Liouville's equation(FTLE).The FTLE method has been validated not only with 6-h sampled synthetic excitation series but also with daily and 6-h sampled polar motion measurements as well asχ(t)produced by the interactive webpage tool of the International Earth Rotation and Reference Systems Service(IERS).Numerical comparisons demonstrate thatχ(t)derived from the FTLE method has superior performances in both the time and frequency domains with respect to that obtained from the widely adopted method or the IERS webpage tool,provided that the input p(t)series has a length around or more than 25 years,which presents no practical limitations since the necessary polar motion data are readily available.The FTLE code is provided in the form of Mat Lab function.
基金This work is funded in part by the Science and Technology Development Fund,Macao SAR(Grant Nos.0093/2022/A2,0076/2022/A2 and 0008/2022/AGJ)in part by the National Nature Science Foundation of China(Grant No.61872452)+3 种基金in part by Special fund for Dongguan’s Rural Revitalization Strategy in 2021(Grant No.20211800400102)in part by Dongguan Special Commissioner Project(Grant No.20211800500182)in part by Guangdong-Dongguan Joint Fund for Basic and Applied Research of Guangdong Province(Grant No.2020A1515110162)in part by University Special Fund of Guangdong Provincial Department of Education(Grant No.2022ZDZX1073).
文摘Interference management is one of the most important issues in the device-to-device(D2D)-enabled heterogeneous cellular networks(HetCNets)due to the coexistence of massive cellular and D2D devices in which D2D devices reuse the cellular spectrum.To alleviate the interference,an efficient interference management way is to set exclusion zones around the cellular receivers.In this paper,we adopt a stochastic geometry approach to analyze the outage probabilities of cellular and D2D users in the D2D-enabled HetCNets.The main difficulties contain three aspects:1)how to model the location randomness of base stations,cellular and D2D users in practical networks;2)how to capture the randomness and interrelation of cellular and D2D transmissions due to the existence of random exclusion zones;3)how to characterize the different types of interference and their impacts on the outage probabilities of cellular and D2D users.We then run extensive Monte-Carlo simulations which manifest that our theoretical model is very accurate.
文摘BACKGROUND Kinesiophobia is a common condition often manifested in patients with musculoskeletal disorders within the process of rehabilitation.Recently,the literature has been investigating whether Pilates could contribute to the management of kinesiophobia in various musculoskeletal disorders.However,aggregated data regarding its effectiveness are absent from literature.AIM To evaluate recordings of the Pilates method in kinesiophobia related to musculoskeletal disorders.METHODS PubMed,ScienceDirect,Scopus and Pedro databases were all scrutinized for randomized controlled trials,by two or more intervention groups,where at least one group received a Pilates-based intervention and which had been conducted in patients aged 18-65 years with musculoskeletal disorders,having assessed at least one outcome related to kinesiophobia.The systematic review was based on the PRISMA guidelines.RESULTS We have identified five studies,with a total of 366 patients with musculoskeletal disorders.Three of them showed that a Pilates-based intervention by either mat or equipment can combat kinesiophobia in patients with musculoskeletal conditions,while another showed that Pilates exercises with equipment may have better long-term effects on kinesiophobia compared to Pilates mat.CONCLUSION Overall,a strong level of research evidence has been amassed for the Pilates intervention as well as a moderate level of research evidence for the effectiveness of equipment-based Pilates in reducing kinesiophobia in patients with musculoskeletal disorders.While the underlying mechanisms driving such a result remain unknown,it appears that Pilates can influence both biological and psychological factors in musculoskeletal disorders,thus resulting in the management of kinesiophobic behaviours.
