mCLCA3 is a member of calcium activated chloride channel（CACC） family that may play an important role in mucin packaging and secretion in asthmatic and cystic fibrosis lung. To study the protein structure and expres...mCLCA3 is a member of calcium activated chloride channel（CACC） family that may play an important role in mucin packaging and secretion in asthmatic and cystic fibrosis lung. To study the protein structure and expression of mCLCA3 in asthmatic mouse lung, an N-terminal 269 amino acid peptide of mCLCA3 was expressed in E. coli, purified to homogeneity and rabbit polyclonal antibodies against this peptide were generated. Immunohistochemistry of asthmatic mouse lung using the antibody indicated exclusive mCLCA3 expression in mucin granules of goblet cells in airway surface and lumen, Immunoblot analysis of lavage fluid from asthmatic mouse lung revealed a single 90 kDa protein form of mClCA3. The results demonstrate that the 90 kDa N-terminal peptide, neither the flail-length protein nor the reported N-terminal 35 kDa cleaved form of mClCA3 is the major functional form involved in the packaging and exocytosis of mucin granules in asthmatic goblet cells.展开更多
Asthma is a worldwide prevalent disease that is a .considerable health burden in many countries. In recent years, the airway epithelium is increasingly recognized as a central contributor to the pathogenesis of asthma...Asthma is a worldwide prevalent disease that is a .considerable health burden in many countries. In recent years, the airway epithelium is increasingly recognized as a central contributor to the pathogenesis of asthma. One of the most highly induced genes in epithelial ceils in experimental allergic airway disease is the third murine calcium-activated chloride channel homologue （mclca3, alias gob-5）. Its human homology protein is hCLCA, which has been identified as clinically relevant molecules in diseases with secretory dysfunctions including asthma and cystic fibrosis.展开更多
基金Supported by the Distinguished Young Scholars Fund of China(No.30325011)the National Natural Science Foundation of China(Nos.30470405and30670477)+1 种基金Distinguished Young Scholars Fund of Jilin Province,China(No.20030112)Excellent Young Teachers Program of MOE,China.
文摘mCLCA3 is a member of calcium activated chloride channel（CACC） family that may play an important role in mucin packaging and secretion in asthmatic and cystic fibrosis lung. To study the protein structure and expression of mCLCA3 in asthmatic mouse lung, an N-terminal 269 amino acid peptide of mCLCA3 was expressed in E. coli, purified to homogeneity and rabbit polyclonal antibodies against this peptide were generated. Immunohistochemistry of asthmatic mouse lung using the antibody indicated exclusive mCLCA3 expression in mucin granules of goblet cells in airway surface and lumen, Immunoblot analysis of lavage fluid from asthmatic mouse lung revealed a single 90 kDa protein form of mClCA3. The results demonstrate that the 90 kDa N-terminal peptide, neither the flail-length protein nor the reported N-terminal 35 kDa cleaved form of mClCA3 is the major functional form involved in the packaging and exocytosis of mucin granules in asthmatic goblet cells.
文摘Asthma is a worldwide prevalent disease that is a .considerable health burden in many countries. In recent years, the airway epithelium is increasingly recognized as a central contributor to the pathogenesis of asthma. One of the most highly induced genes in epithelial ceils in experimental allergic airway disease is the third murine calcium-activated chloride channel homologue （mclca3, alias gob-5）. Its human homology protein is hCLCA, which has been identified as clinically relevant molecules in diseases with secretory dysfunctions including asthma and cystic fibrosis.
