In the present paper, an attempt is made to obtain the degree of approximation of conjugate of functions (signals) belonging to the generalized weighted W(LP, ξ(t)), (p ≥ 1)-class, by using lower triangular matrix o...In the present paper, an attempt is made to obtain the degree of approximation of conjugate of functions (signals) belonging to the generalized weighted W(LP, ξ(t)), (p ≥ 1)-class, by using lower triangular matrix operator of conjugate series of its Fourier series.展开更多
Natural polyamine is an ideal antitumor drug carrier because of the great requirement difference between cancer cells and normal cells. Previous data demonstrated that many cytotoxic drugs conjugated with natural or s...Natural polyamine is an ideal antitumor drug carrier because of the great requirement difference between cancer cells and normal cells. Previous data demonstrated that many cytotoxic drugs conjugated with natural or synthetic polyamines have potent antitumor effects. Up to now, the antitumor mechanism of conjugates of naphthalimides with polyamine remains poorly understood in human colon cancer cells. The aim of this study is to evaluate the effect of MINS (a novel naph-thalimide-polyamine conjugate) and mechanism of MINS in human colon cancer cells. Mitochondrial toxicity, which was generated by ROS from mitochondria electron transport chain, might be a major factor in MINS-inducted apoptosis. Our data also demonstrated that MINS-mediated cell apoptosis depend on p53 status, for MINS induced Caco-2 cells (p53 null) only necrosis but not apoptosis. Furthermore, the apoptotic effect of MINS is stronger than that of Amonafide, the parent drug of MINS. Our data suggested that the MINS-mediated cell apoptosis depend on p53 in human colon cancer cells.展开更多
AIM: To determine the effect of cis -9, trans -11-conjugated linoleic acid (c9, t11-CLA) on the cell cycle of gastric cancer cells (SGC-7901) and its possible mechanism in inhibition cancer growth. METHODS: Using cell...AIM: To determine the effect of cis -9, trans -11-conjugated linoleic acid (c9, t11-CLA) on the cell cycle of gastric cancer cells (SGC-7901) and its possible mechanism in inhibition cancer growth. METHODS: Using cell culture and immunocytochemical techniques, we examined the cell growth, DNA synthesis, expression of PCNA, cyclin A, B(1), D(1), p16(ink4a) and p21(cip/waf1) of SGC-7901 cells which were treated with various c9, t11-CLA concentrations (25, 50, 100 and 200 micromol.L(-1))of c 9, t 11-CLA for 24 and 48h, with a negative control (0.1% ethane). RESULTS: The cell growth and DNA synthesis of SGC-7901 cells were inhibited by c9, t11-CLA.SGC-7901 cells. Eight day after treatment with various concentrations of c9, t11-CLA mentioned above, the inhibition rates were 5.92%, 20.15%, 75.61% and 82.44%, respectively and inhibitory effect of c9, t11-CLA on DNA synthesis (except for 25 micromol.L, 24h) showed significantly less (3)H-TdR incorporation than that in the negative controls (P【0.05 and P【0.01). Immunocytochemical staining demonstrated that SGC-7901 cells preincubated in media supplemented with different c9, t11-CLA concentrations at various times significantly decreased the expressions of PCNA (the expression rates were 7.2-3.0%, 24h and 9.1-0.9% at 48h, respectively), Cyclin A (11.0-2.3%, 24h and 8.5-0.5%,48h), B(1) (4.8-1.8% at 24h and 5.5-0.6% at 48h)and D(1) (3.6-1.4% at 24h and 3.7%-0 at 48h) as compared with those in the negative controls(the expressions of PCNA, Cyclin A, B(1) and D(1) were 6.5% at 24h and 9.0% at 48h, 4.2% at 24h and 5.1% at 48h, 9.5% at 24h and 6.0% at 48h,respectively)(P【0.01), whereas the expressions of P16(ink4a) and P21(cip/waf1), cyclin-dependent kinases inhibitors(CDKI), were increased. CONCLUSION: The cell growth and proliferation of SGC-7901 cell is inhibited by c9, t11-CLA via blocking the cell cycle, with reduced expressions of cyclin A,B(1) and D(1) and enhanced expressions of CDKI(P16(ink4a) and p21(cip/waf1)).展开更多
Antimicrobial susceptibility test was performed on 57 clinical isolates of P. aeruginosa and 36 clinical isolates of Acinetobacter with 11 antimicrobial agents including getamicin, amikacin, ciprofloxacin, ofloxacin, ...Antimicrobial susceptibility test was performed on 57 clinical isolates of P. aeruginosa and 36 clinical isolates of Acinetobacter with 11 antimicrobial agents including getamicin, amikacin, ciprofloxacin, ofloxacin, fleroxacin, piperacillin, cefotaxime, cefoperazone/sulbactam, ceftazidime, cefoperazone and doxycycline. Transferable drug resistance plasmid carrying rates of these clinical isolates were also studied. On the basis of the in vitro activities, 52.63%(30/57) of the isolated strains of P. aeruginosa were susceptible to antimicrobial agents selected (except doxycycline), 41.67%(15/36) of the isolated strains of Acinetobacter were susceptible to 11 antimicrobial agents. The sensitivity rate of P.aeruginosa and Acinetobacter to antimicrobial agents selected was 70% or greater to all except doxycycline. Furthermore, the sensitivity rate of P.aeruginosa to amikacin ciprofloxacin, ceftazidime, cefoperazone, cefoperazone/sulbactam, and that of Acinetobacter to cefoperazone/sulbactam, amikacin was more than 90%,among them amikacin, cefoperazone/sulbactam being the most effective. Plasmid analysis showed that 15.79%(9/57) P.aeruginosa strains and 13.89%(5/36) Acinetobacter strains carried plasmid. Conjugative plasmid carrying rates of P. aeruginosa strains and Acinetobacter strains were 7.02%(4/57), 13.89%(5/36), respectively. Conjugative plasmid didn′t play an important role in the formation and dissemination of drug resistance of P. aeruginosa and Acinetobacter.展开更多
Homoclinic solutions were introduced for a prescribed mean curvature Lienard p-Laplacian equation with a deviating argument.It was divided into three parts to discuss the existence of homoclinic solutions.By using an ...Homoclinic solutions were introduced for a prescribed mean curvature Lienard p-Laplacian equation with a deviating argument.It was divided into three parts to discuss the existence of homoclinic solutions.By using an extension of Mawhin's continuation theorem,the existence of a set with 2kT-periodic for a prescribed mean curvature Lienard p-Laplacian equation with a deviating argument was studied.According to a limit on a certain subsequence of 2kT-periodic set,homoclinic solutions were obtained.A numerical example demonstrates the validity of the main results.展开更多
The mean activity coefficient of 5, 10,15 , 20-tetrakis (P-methoxyl-O-sulfophenyl)porphyrin sodium in dilute aqueous solution has been determined in the modality range 0. 00547-0. 08871 mol · kg-1at 273. 2 K by t...The mean activity coefficient of 5, 10,15 , 20-tetrakis (P-methoxyl-O-sulfophenyl)porphyrin sodium in dilute aqueous solution has been determined in the modality range 0. 00547-0. 08871 mol · kg-1at 273. 2 K by the freezing-point depression method . The results of γ± are 0. 9945-0. 7695, it is in close agreement with that by isopiestic method.展开更多
RSRP(Reference Signal Receiving Power)是代表无线信号强度的关键参数,也是物理层测量的重要指标之一。当前5G NR中RSRP的测量算法多是均值法和其衍生算法,通过对现有算法的研究提出一种基于子集的共轭相乘算法。根据均值算法和共轭...RSRP(Reference Signal Receiving Power)是代表无线信号强度的关键参数,也是物理层测量的重要指标之一。当前5G NR中RSRP的测量算法多是均值法和其衍生算法,通过对现有算法的研究提出一种基于子集的共轭相乘算法。根据均值算法和共轭相乘算法进行测量值估计,并对相应算法进行测量结果的比较,进而得到子集共轭相乘算法在不同信噪比情况下可以使用不同子集数来提高RSRP估计值。将提出的新算法结合5G系统的特点与均值算法和共轭相乘算法进行比较分析,得到三种算法各自的特点。展开更多
文摘In the present paper, an attempt is made to obtain the degree of approximation of conjugate of functions (signals) belonging to the generalized weighted W(LP, ξ(t)), (p ≥ 1)-class, by using lower triangular matrix operator of conjugate series of its Fourier series.
文摘Natural polyamine is an ideal antitumor drug carrier because of the great requirement difference between cancer cells and normal cells. Previous data demonstrated that many cytotoxic drugs conjugated with natural or synthetic polyamines have potent antitumor effects. Up to now, the antitumor mechanism of conjugates of naphthalimides with polyamine remains poorly understood in human colon cancer cells. The aim of this study is to evaluate the effect of MINS (a novel naph-thalimide-polyamine conjugate) and mechanism of MINS in human colon cancer cells. Mitochondrial toxicity, which was generated by ROS from mitochondria electron transport chain, might be a major factor in MINS-inducted apoptosis. Our data also demonstrated that MINS-mediated cell apoptosis depend on p53 status, for MINS induced Caco-2 cells (p53 null) only necrosis but not apoptosis. Furthermore, the apoptotic effect of MINS is stronger than that of Amonafide, the parent drug of MINS. Our data suggested that the MINS-mediated cell apoptosis depend on p53 in human colon cancer cells.
基金the National Natural Science Foundation of China,No.39870661
文摘AIM: To determine the effect of cis -9, trans -11-conjugated linoleic acid (c9, t11-CLA) on the cell cycle of gastric cancer cells (SGC-7901) and its possible mechanism in inhibition cancer growth. METHODS: Using cell culture and immunocytochemical techniques, we examined the cell growth, DNA synthesis, expression of PCNA, cyclin A, B(1), D(1), p16(ink4a) and p21(cip/waf1) of SGC-7901 cells which were treated with various c9, t11-CLA concentrations (25, 50, 100 and 200 micromol.L(-1))of c 9, t 11-CLA for 24 and 48h, with a negative control (0.1% ethane). RESULTS: The cell growth and DNA synthesis of SGC-7901 cells were inhibited by c9, t11-CLA.SGC-7901 cells. Eight day after treatment with various concentrations of c9, t11-CLA mentioned above, the inhibition rates were 5.92%, 20.15%, 75.61% and 82.44%, respectively and inhibitory effect of c9, t11-CLA on DNA synthesis (except for 25 micromol.L, 24h) showed significantly less (3)H-TdR incorporation than that in the negative controls (P【0.05 and P【0.01). Immunocytochemical staining demonstrated that SGC-7901 cells preincubated in media supplemented with different c9, t11-CLA concentrations at various times significantly decreased the expressions of PCNA (the expression rates were 7.2-3.0%, 24h and 9.1-0.9% at 48h, respectively), Cyclin A (11.0-2.3%, 24h and 8.5-0.5%,48h), B(1) (4.8-1.8% at 24h and 5.5-0.6% at 48h)and D(1) (3.6-1.4% at 24h and 3.7%-0 at 48h) as compared with those in the negative controls(the expressions of PCNA, Cyclin A, B(1) and D(1) were 6.5% at 24h and 9.0% at 48h, 4.2% at 24h and 5.1% at 48h, 9.5% at 24h and 6.0% at 48h,respectively)(P【0.01), whereas the expressions of P16(ink4a) and P21(cip/waf1), cyclin-dependent kinases inhibitors(CDKI), were increased. CONCLUSION: The cell growth and proliferation of SGC-7901 cell is inhibited by c9, t11-CLA via blocking the cell cycle, with reduced expressions of cyclin A,B(1) and D(1) and enhanced expressions of CDKI(P16(ink4a) and p21(cip/waf1)).
文摘Antimicrobial susceptibility test was performed on 57 clinical isolates of P. aeruginosa and 36 clinical isolates of Acinetobacter with 11 antimicrobial agents including getamicin, amikacin, ciprofloxacin, ofloxacin, fleroxacin, piperacillin, cefotaxime, cefoperazone/sulbactam, ceftazidime, cefoperazone and doxycycline. Transferable drug resistance plasmid carrying rates of these clinical isolates were also studied. On the basis of the in vitro activities, 52.63%(30/57) of the isolated strains of P. aeruginosa were susceptible to antimicrobial agents selected (except doxycycline), 41.67%(15/36) of the isolated strains of Acinetobacter were susceptible to 11 antimicrobial agents. The sensitivity rate of P.aeruginosa and Acinetobacter to antimicrobial agents selected was 70% or greater to all except doxycycline. Furthermore, the sensitivity rate of P.aeruginosa to amikacin ciprofloxacin, ceftazidime, cefoperazone, cefoperazone/sulbactam, and that of Acinetobacter to cefoperazone/sulbactam, amikacin was more than 90%,among them amikacin, cefoperazone/sulbactam being the most effective. Plasmid analysis showed that 15.79%(9/57) P.aeruginosa strains and 13.89%(5/36) Acinetobacter strains carried plasmid. Conjugative plasmid carrying rates of P. aeruginosa strains and Acinetobacter strains were 7.02%(4/57), 13.89%(5/36), respectively. Conjugative plasmid didn′t play an important role in the formation and dissemination of drug resistance of P. aeruginosa and Acinetobacter.
基金Natural Foundation of Wuyi University,China(No.XQ201305)Young-Middle-Aged Teachers Education Scientific Research Project in Fujian Province,China(No.JA15524)
文摘Homoclinic solutions were introduced for a prescribed mean curvature Lienard p-Laplacian equation with a deviating argument.It was divided into three parts to discuss the existence of homoclinic solutions.By using an extension of Mawhin's continuation theorem,the existence of a set with 2kT-periodic for a prescribed mean curvature Lienard p-Laplacian equation with a deviating argument was studied.According to a limit on a certain subsequence of 2kT-periodic set,homoclinic solutions were obtained.A numerical example demonstrates the validity of the main results.
文摘The mean activity coefficient of 5, 10,15 , 20-tetrakis (P-methoxyl-O-sulfophenyl)porphyrin sodium in dilute aqueous solution has been determined in the modality range 0. 00547-0. 08871 mol · kg-1at 273. 2 K by the freezing-point depression method . The results of γ± are 0. 9945-0. 7695, it is in close agreement with that by isopiestic method.
文摘RSRP(Reference Signal Receiving Power)是代表无线信号强度的关键参数,也是物理层测量的重要指标之一。当前5G NR中RSRP的测量算法多是均值法和其衍生算法,通过对现有算法的研究提出一种基于子集的共轭相乘算法。根据均值算法和共轭相乘算法进行测量值估计,并对相应算法进行测量结果的比较,进而得到子集共轭相乘算法在不同信噪比情况下可以使用不同子集数来提高RSRP估计值。将提出的新算法结合5G系统的特点与均值算法和共轭相乘算法进行比较分析,得到三种算法各自的特点。
