The study objects includes seven swine breeds： Minzhu, Sanjiangbaizhu, Yorkshire, Landrace, Junmuyihao, Duroc and Double muscle Yorkshire. According to the sequences of MyoG, MyoD and Myf5 of swine in GenBank, sevent...The study objects includes seven swine breeds： Minzhu, Sanjiangbaizhu, Yorkshire, Landrace, Junmuyihao, Duroc and Double muscle Yorkshire. According to the sequences of MyoG, MyoD and Myf5 of swine in GenBank, seventeen pairs of primers for MyoG, MyoD and Myf5 were designed. PCR-SSCP technology was applied to detect SNPs of the exons of the three genes. The results showed that no polymorphism was in MyoG and MyoD, and some SNPs were in three exons of Myf5. There was one mutant site in the first exon of Myf5 （G → C）, three mutant sites in the second exon of Myf5 （C → A, A → G and G → A）; in the third exon of Myf5, there was one base A deficiency at 3 387 bp, three bases T deficiency at 3417 bp, one mutant site at 3443 bp （T → C）. This study obtained a tendency conclusion that gene frequency of allele M of Myf5 on the one hand is positively correlated with lean meat percentage, on the other hand is correlated with the orientation of selective breeding; it also deduced that allele F is possibly correlated with high lean meat percentage. Through statistical analysis, allele A, B, C of Myf5 have no obvious correlation with lean meat percentage of different swine breeds. In addition, the high polymorphism of Myf5 showed that seven swine breeds are rich in genetic variation, and have high selective competency.展开更多
[ Objective] This study aimed to investigate the slaughter performance of transgenic pigs harboring IGF-I gene under tetracycline induction. [ Method ] Pigs were given diets with the addition of tetracycline. After 45...[ Objective] This study aimed to investigate the slaughter performance of transgenic pigs harboring IGF-I gene under tetracycline induction. [ Method ] Pigs were given diets with the addition of tetracycline. After 45 d of tetracycline induction, experimental pigs were weighed and slaughtered for parameter determina- tion. [ Result] The lean meat percentage of experimental pigs was improved by 8.92%, but various blood biochemical parameters and carcass components exhibited no significant changes. [ Conclusion ] Under tetracycline induction, transganic pigs harboring IGF-I gane demonstrated an increase in lean meat percentage without abnormal changes in other parameters.展开更多
文摘The study objects includes seven swine breeds： Minzhu, Sanjiangbaizhu, Yorkshire, Landrace, Junmuyihao, Duroc and Double muscle Yorkshire. According to the sequences of MyoG, MyoD and Myf5 of swine in GenBank, seventeen pairs of primers for MyoG, MyoD and Myf5 were designed. PCR-SSCP technology was applied to detect SNPs of the exons of the three genes. The results showed that no polymorphism was in MyoG and MyoD, and some SNPs were in three exons of Myf5. There was one mutant site in the first exon of Myf5 （G → C）, three mutant sites in the second exon of Myf5 （C → A, A → G and G → A）; in the third exon of Myf5, there was one base A deficiency at 3 387 bp, three bases T deficiency at 3417 bp, one mutant site at 3443 bp （T → C）. This study obtained a tendency conclusion that gene frequency of allele M of Myf5 on the one hand is positively correlated with lean meat percentage, on the other hand is correlated with the orientation of selective breeding; it also deduced that allele F is possibly correlated with high lean meat percentage. Through statistical analysis, allele A, B, C of Myf5 have no obvious correlation with lean meat percentage of different swine breeds. In addition, the high polymorphism of Myf5 showed that seven swine breeds are rich in genetic variation, and have high selective competency.
基金Supported by Key Project on GMO Cultivation for New Varieties of China(2014ZX08006-003,2014ZX08010-3)National Natural Science Foundation of China(31201790)+1 种基金Project of Hubei Agricultural Science and Technology Innovation Center(2011-620-001-003)Science and Technology Support Program of Hubei Province(2014BBB010)
文摘[ Objective] This study aimed to investigate the slaughter performance of transgenic pigs harboring IGF-I gene under tetracycline induction. [ Method ] Pigs were given diets with the addition of tetracycline. After 45 d of tetracycline induction, experimental pigs were weighed and slaughtered for parameter determina- tion. [ Result] The lean meat percentage of experimental pigs was improved by 8.92%, but various blood biochemical parameters and carcass components exhibited no significant changes. [ Conclusion ] Under tetracycline induction, transganic pigs harboring IGF-I gane demonstrated an increase in lean meat percentage without abnormal changes in other parameters.
