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Small-scale fire tests in the underwater tunnel section model with new sidewall smoke extraction
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作者 Shunyu Yue Ruifeng Miao +2 位作者 Huihang Cheng Maohua Zhong Xiujun Yang 《Deep Underground Science and Engineering》 2024年第2期247-254,共8页
The Shenzhen–Zhongshan Bridge is a 24‐km‐long bridge and tunnel system,including a 6.8-km-long super cross section subsea tunnel.To solve the smoke exhaust problem of a super large cross-section subsea tunnel,the t... The Shenzhen–Zhongshan Bridge is a 24‐km‐long bridge and tunnel system,including a 6.8-km-long super cross section subsea tunnel.To solve the smoke exhaust problem of a super large cross-section subsea tunnel,the tunnel has a new smoke exhaust system that combines a horizontal smoke exhaust cross section at the top and sidewall smoke exhaust holes.In order to evaluate the potential fire hazards of this type of tunnel,a 1:30 tunnel model was established and 140 smallscale experiments on underwater tunnel fires were conducted.By changing the fire power,fire location,and fan operation mode,different scenarios of submarine immersed tunnel fire were simulated and the related key parameters such as fire smoke diffusion behavior and smoke temperature distribution were studied.On this basis,the optimal smoke control strategy was proposed for different fire scenarios.The research results indicate that the new smoke exhaust system can fully utilize the smoke flow characteristics,significantly improve smoke exhaust efficiency,and increase available evacuation time,thus further enhancing the fire safety of super large cross-section subsea tunnels. 展开更多
关键词 sidewall smoke extraction system small‐scale fire tests smoke control
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Dysregulation of gastric H,K-ATPase by cigarette smoke extract 被引量:7
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作者 Muna Hammadi Mohamed Adi +2 位作者 Rony John Ghalia AK Khoder Sherif M Karam 《World Journal of Gastroenterology》 SCIE CAS CSCD 2009年第32期4016-4022,共7页
AIM:To test whether the expression and activity of H,K-ATPase in parietal cells would be affected by cigarette smoke extract.METHODS: Extracts of cigarette smoke were administered into mice by gastric gavage (5 mg/kg ... AIM:To test whether the expression and activity of H,K-ATPase in parietal cells would be affected by cigarette smoke extract.METHODS: Extracts of cigarette smoke were administered into mice by gastric gavage (5 mg/kg body weight/day) for 3 d or in drinking water for 7 or 14 d. For the latter, each day a mouse consumed 5 mL water containing extracts of two cigarettes, on average. Control littermate mice received only vehicle. To compare the amount of H,K-ATPase in control and smoke-treated mice, the stomach was processed for Western blotting and immunohistochemical analysis using monoclonal antibodies specific for α- or β-subunits of H,K-ATPase. The p-nitrophenylphospatase activity assay was used as a measurement for K-dependent H,K-ATPase activity.RESULTS: Probed transblots showed an increase in the amount of H,K-ATPase in smoke-treated mice which was confirmed by immunohistochemistry and was found to be due to increased amounts of protein per parietal cell rather than an increased parietal cell number. The increase in the amount of H,K-ATPase was associated with an enhancement of its enzymatic activity. K-dependent activity in control and smoke-treated mice was significantly different (respectively, 0.12 μmol/mg vs 0.27 μmol/mg per minute, P<0.05).CONCLUSION: Administration of cigarette smoke extract is associated with an increase in the amount and activity of H,K-ATPase and hence, smokers are susceptible to development of peptic ulcer. 展开更多
关键词 Proton pump H K-ATPase Parietal cell Gastric gland Oxyntic mucosa Cigarette smoke extract Smoking
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Berberine Attenuates Cigarette Smoke Extract-induced Airway Inflammation in Mice:Involvement of TGF-β1/Smads Signaling Pathway 被引量:6
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作者 Wen WANG Gan ZHA +3 位作者 Jin-jing ZOU Xun WANG Chun-nian LI Xiao-jun WU 《Current Medical Science》 SCIE CAS 2019年第5期748-753,共6页
Although several studies confirmed that berberine may attenuate airway inflammation in mice with chronic obstructive pulmonary disease(COPD),its underlying mechanisms were not clear until now.We aimed to establish an ... Although several studies confirmed that berberine may attenuate airway inflammation in mice with chronic obstructive pulmonary disease(COPD),its underlying mechanisms were not clear until now.We aimed to establish an experiment mouse model for COPD and to investigate the effects of berberine on airway inflammation and its possible mechanism in COPD model mice induced by cigarette smoke extract(CSE).Twenty SPF C57BL/6 mice were randomly divided into PBS control group,COPD model group,low-dose berberine group and high-dose berberine group,5 mice in each group.The neutrophils and macrophages were examined by Wright's staining.The levels of inflammatory cytokines TNF-α and IL-6 in bronchoalveolar lavage fluid(BALF)were detennined by enzyme-linked immunosorbent assay.The expression levels of TGF-β1,Smad2 and Smad3 mRNA and proteins in lung tissues were respectively detected by quantitative real-time polymerase chain reaction and Western blotting.It was found that CSE increased the number of inflammation cells in BALF,elevated lung inflammation scores,and enhanced the TGF-β1/Smads signaling activity in mice.High-dose berberine restrained the alterations in the COPD mice induced by CSE.It was concluded that high-dose berberine ameliorated CSE-induced airway inflammation in COPD mice.TGF-β1/Smads signaling pathway might be involved in the mechanism.These findings suggested a therapeutic potential of high-dose berberine on the CSE-induced airway inflammation. 展开更多
关键词 BERBERINE CIGARETTE smoke extract chronic OBSTRUCTIVE pulmonary disease TGF-β1/Smads signaling pathway
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Damaging Effect of Cigarette Smoke Extract on PrimaryCultured Human Umbilical Vein Endothelial Cells and Its Mechanism 被引量:4
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作者 Yu-MEIYANG GENG-TAOLIU 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2004年第2期121-134,共14页
Objective To investigate the cellular effects of cigarette smoke extract (CSE) on primarily cultured human umbilical vein endothelial cells (HUVEC). Methods The effects of CSE (5%-20%) and nicotine (10-4 mol/L) on HUV... Objective To investigate the cellular effects of cigarette smoke extract (CSE) on primarily cultured human umbilical vein endothelial cells (HUVEC). Methods The effects of CSE (5%-20%) and nicotine (10-4 mol/L) on HUVEC viability, proliferation, angiogenesis and apoptosis were observed. Results CSE decreased HUVEC survival rate and angiogenesis after 24 h as well as its proliferation after 48 h in a dose-dependent manner. Moreover, CSE induced apoptosis of HUVEC as indicated in condensation of nuclear chromatin and the presence of hypodiploid DNA. HUVEC incubated with CSE for 24 h gave a significant decrease in the expression of Bcl-2 as well as the decline in the Bcl-2/Bax ratio accompanied with the loss of mitochondrial membrane potential and excess cytosolic calcium. Our study also observed that p53 protein level decreased, rather than increased in cells treated with CSE. Nicotine had no discernible inhibitory effects on the above indices of HUVEC. Conclusion Exposure to CSE other than nicotine causes inhibition of viability, proliferation and differentiation of HUVEC. CSE-induced HUVEC injury is mediated in part through accelerated apoptosis but independent of p53 pathway. It appears that mitochondria have played a key role in the apoptosis of HUVEC induced by CSE. 展开更多
关键词 Cigarette smoke extracts (CSE) Human umbilical endothelial cell (HUVEC) VIABILITY Proliferation ANGIOGENESIS Mitochondrial membrane potential Cytosolic calcium Bcl-2 BCL-2/BAX p53
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Effect of Cigarette Smoke Extract on the Role of Protein Kinase C in the Proliferation of Passively Sensitized Human Airway Smooth Muscle Cells 被引量:2
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作者 林俊岭 徐永健 +2 位作者 张珍祥 倪望 陈仕新 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2005年第3期269-273,共5页
To investigate the effect of cigarette smoke extract (CSE) on the role of protein kinase C (PKC) in the proliferation of passively sensitized human airway smooth muscle cells (HASMCs). After synchronization of culture... To investigate the effect of cigarette smoke extract (CSE) on the role of protein kinase C (PKC) in the proliferation of passively sensitized human airway smooth muscle cells (HASMCs). After synchronization of cultured HASMCs, they were divided into a group A and Group B. The group A was treated with normal human serum and served as controls and the group B was treated with the serum of asthma patients. The group A was further divided into group of A_1, A_2 and A_3 and the group B was sub-divided into the group of B_1, B_2, B_3, B_4 and B_5. No other agents were added to the group A_1 and B_1. The cells of group A_2 and B_2 were stimulated with 5 % CSE for 24 h. HASMCs from group A_3 and B_3 were treated with PKC agonist PMA (10 nmol/L) and CSE (5 %) for 24 h. PKC inhibitor Ro-31-8220 (5 μmol/L) was added to the HASMCs of group B_4 for 24 h. The cells from group B_5 were stimulated with Ro-31-8220 (5 μmol/L) and CSE (5 %) for 24 h. The proliferation of HASMCs isolated from group A and B was examined by cell cycle analysis, MTT colorimetric assay and 3H-TdR incorporation test. The expression of PKC-α in each group was observed by Western blotting and RT-PCR, respectively. The results showed that the percentage of S phase, absorbance (A) value, the rate of 3H-TdR incorporation, the ratios of A value of PKC-α mRNA and the A value of PKC-α protein in HASMCs from group B_1, B_2 and B_3 were significantly increased compared to those of group A_1, A_2 and A_3 correspondingly and respectively (P<0.01). The proliferation of HASMCs of group A_2 and B_2 stimulated with CSE and group A_3 and B_3 stimulated with CSE and PMA were also significantly enhanced when group A_1, A_2 and A_3 and group B_1, B_2 and B_3 compared to each other (P<0.05, P<0.01, respectively). The percentage of S phase, absorbency (A) value, 3H-TdR incorporation rate, the ratios of A value of PKC-α mRNA and the A value of PKC-α protein in HASMCs from group B_4 treated with Ro-31-8220 and group B_5 treated with CSE and Ro-31-8220 were significantly decreased as compared to those of group B_1 and B_2 correspondingly and respectively (P<0.05, P<0.01). It was concluded that CSE can enhance the passively sensitized HASMC proliferation and the expression of PKC alpha. PKC and its alpha subtype may contribute to this process. Our results suggest cigarette may play an important role in ASMCs proliferation of asthma through PKC signal pathway. 展开更多
关键词 cigarette smoke extract protein kinase C ASTHMA airway smooth muscle cells PROLIFERATION
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Injury of Mouse Brain Mitochondria Induced by Cigarette Smoke Extract and Effect of Vitamin C on It in vitro 被引量:1
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作者 YU-MEI YANG AND GENG-TAO LIUDivision of Pharmacology, Institute of Materia Medica, Chinese Academy of Medical Sciences, Peking Union Medical College, 1 Xian Nong Tan Street, Beijing 100050, China 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2003年第3期256-266,共11页
Objective To investigate the toxicity of cigarette smoke extract (CSE) and nicotine on mouse brain mitochondria as well as the protective effect of vitamin C in vitro. Method Mouse brain mitochondria in vitro was incu... Objective To investigate the toxicity of cigarette smoke extract (CSE) and nicotine on mouse brain mitochondria as well as the protective effect of vitamin C in vitro. Method Mouse brain mitochondria in vitro was incubated with CSE or nicotine in the absence or presence of vitamin C for 60 minutes, and the changes of mitochondrial function and structure were measured. Results CSE inhibited mitochondrial ATPase and cytochrome C oxidase activities in a dose-dependent manner. However, no significant changes in the peroxidation indices were observed when mitochondrial respiratory enzymes activity was inhibited, and protection of mitochondria from CSE-induced injury by vitamin C was not displayed in vitro. The effect of CSE on mouse brain mitochondria swelling response to calcium stimulation was dependent on calcium concentrations. CSE inhibited swelling of mitochondria at 6.5μmol/L Ca2+, but promoted swelling response at 250μmol/L Ca2+. Nicotine, the major component of cigarette smoke, showed no significant damage in mouse brain mitochondria in vitro. The CSE treatment induced mitochondrial inner membrane damage and vacuolization of the matrix, whereas the outer mitochondrial membrane appeared to be preserved. Conclusion The toxic effect of CSE on brain mitochondria may be due to its direct action on enzymatic activity rather than through oxygen free radical injury. Nicotine is not the responsible component for the toxicity of CSE to brain mitochondria. 展开更多
关键词 Cigarette smoke extract NICOTINE Vitamin C Mitochondrial function Mitochondria! structure
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Roles of TGF-β Signaling Pathway in Endoplasmic Reticulum Stress in Endothelial Cells Stimulated with Cigarette Smoke Extract 被引量:1
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作者 黄宏 丁秋丽 +1 位作者 朱慧芬 杨道锋 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2017年第5期699-704,共6页
To investigate the role of signaling pathway in the effect of endoplasmic reticulum stress(ER stress) in endothelial cells stimulated with cigarette smoke extract(CSE). Human umbilical vein endothelial cells(HUV... To investigate the role of signaling pathway in the effect of endoplasmic reticulum stress(ER stress) in endothelial cells stimulated with cigarette smoke extract(CSE). Human umbilical vein endothelial cells(HUVECs) were cultured and divided into 3 groups: CSE-stimulated group, CSE-stimulated with 4-PBA group, and negative control group. HUVECs were cultured and stimulated with CSE at concentrations of 5%, 10% and 20%, respectively, mR NA of CXCL-8 and GRP78 was detected by real-time PCR. ELISA was performed to test the expression of CXCL-8 protein, and neutrophils migration was detected by Transwell board test. The NF-κB, ERK, p38 MAPK and transforming growth factor beta(TGF-β) were detected by flow cytometry. The mRNA of CXCL-8 and GRP78 increased in CSE-stimulated HUVECs(P〈0.05). Furthermore, it was concentration-dependent. 4-PBA significantly reduced the expression of CXCL-8 protein(P〈0.05) and neutrophil migration(P〈0.05). The TGF-β, rather than the NF-κB, ERK and P38 MAPK pathway might be involved in ER stress stimulated by CSE. CSE induced neutrophils migration by increasing the expression of CXCL-8 in endothelial cells. ER stress might play a role in the effect of neutrophils migration stimulated with CSE, and TGF-β pathway may contribute to the ER stress in HUVECs. 展开更多
关键词 endoplasmic reticulum stress cigarette smoke extract endothelial cells neutrophil migration signaling pathway
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Cigarette Smoke Extract Inhibits the Proliferation of Alveolar Epithelial Cells and Augments the Expression of P21^(WAF1) 被引量:1
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作者 焦宗宪 敖启林 +1 位作者 葛晓娜 熊密 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2008年第1期6-10,共5页
Cigarette smoking is intimately related with the development of chronic obstructive pulmonary diseases, and alveolar epithelium is a major target for the exposure of cigarette smoke extract. In order to investigate th... Cigarette smoking is intimately related with the development of chronic obstructive pulmonary diseases, and alveolar epithelium is a major target for the exposure of cigarette smoke extract. In order to investigate the effect of cigarette smoke extract on the proliferation of alveolar epithelial cell type Ⅱ and its relationship with P21^WAF1, the alveolar epithelial type Ⅱ cell line (A549) cells were chosen as surrogate cells to represent alveolar epithelial type Ⅱ cells. MTT assay was used to detect cell viability after interfered with different concentrations of cigarette smoke extract. It was observed cigarette smoke extract inhibited the growth of A549 cells in a dose- and time-dependent manner. The morphological changes, involving the condensation and margination of nuclear chromatin, even karyorrhexis, were observed by both Hoechst staining and electronic microscopy. Flow cytometry analysis demonstrated the increased cell percentages in G1 and subG1 phases after the cells were incubated with cigarette smoke extract. The expression of p21^WAF1 protein and mRNA was also significantly increased as detected by the methods of Western blot or reverse transcription-polymerase chain reaction respectively. In conclusion, cigarette smoke extract inhibits the proliferation of alveolar epithelial cell type Ⅱ and blocks them in G1/S phase. The intracelhilar accumulation of P21^WAF1 may be one of the mechanisms which contribute to cigarette smoke extract-induced inhibition of cell proliferation. 展开更多
关键词 cigarette smoke extract alveolar epithelial cell cell proliferation P21^WAF1
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Genotoxicity and Reduced Heat Shock Protein 70 in Human Airway Smooth Muscle Cells Exposed to Cigarette Smoke Extract 被引量:1
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作者 武小杰 罗国雄 +5 位作者 曾雪 兰立立 宁琴 徐永健 赵建平 谢俊刚 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2013年第6期827-833,共7页
Cigarette smoke is associated with the development of several diseases, such as chronic ob- structive pulmonary disease (COPD). The purpose of this study was to investigate genotoxicity and heat shock protein 70 (H... Cigarette smoke is associated with the development of several diseases, such as chronic ob- structive pulmonary disease (COPD). The purpose of this study was to investigate genotoxicity and heat shock protein 70 (Hsp70) in human airway smooth muscle cells (HASMCs) exposed to cigarette smoke extract (CSE). HASMCs was exposed to CSE with different doses for 24 h. The level of 8-hydroxydeoxyguanosine (8-OHdG) was determined by using HPLC-ECD, the DNA damage was ana- lyzed by using comet assay, and apoptosis was examined by using Annexin-FITC/PI staining. The pro- duction of Hsp70 after CSE stimulation was tested. Results indicated that CSE significantly increased the level of 8-OHdG, DNA damage and cell apoptosis, and reduced the production of Hsp70. In par- ticular, levels of Hsp70 were inversely correlated with 8-OHdG, DNA damage and cell apoptosis. It was concluded that cigarette smoke induced genotoxicity and decreased the production of cell protective protein Hsp70, which may contribute to the development of some airway diseases. 展开更多
关键词 airway smooth muscle cigarette smoke extract 8-hydroxydeoxyguanosine DNA damage heat shock protein 70
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Involvement of TRPC1 and Cyclin D1 in Human Pulmonary Artery Smooth Muscle Cells Proliferation Induced by Cigarette Smoke Extract 被引量:1
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作者 Xun WANG Wen WANG +1 位作者 Chan LIU Xiao-jun WU 《Current Medical Science》 SCIE CAS 2020年第6期1085-1091,共7页
Cigarette smoking contributes to the development of pulmonary artery hypertension(PAH).As the basic pathological change of PAH,pulmonary vascular remodeling is considered to be related to the abnormal proliferation of... Cigarette smoking contributes to the development of pulmonary artery hypertension(PAH).As the basic pathological change of PAH,pulmonary vascular remodeling is considered to be related to the abnormal proliferation of pulmonary artery smooth muscle cells(PASMCs).However,the molecular mechanism underlying this process remains not exactly clear.The aim of this research was to study the molecular mechanism of PASMCs proliferation induced by smoking.Human PASMCs(HPASMCs)were divided into 6 groups:0%(control group),cigarette smoking extract(CSE)-treated groups at concentrations of 0.5%,1%,2%,5%,10%CSE respectively.HPASMCs proliferation was observed after 24 h.HPASMCs were divided into two groups:0(control group),0.5%CSE group.The mRNA and protein expression levels of transient receptor potential channel 1(TRPC1)and cyclin D1 in HPASMCs after CSE treatment were respectively detected by RT-PCR and Western blotting.The intracellular calcium ion concentration was measured by the calcium probe in each group.In the negative control group and TRPC1-siRNA transfection group,the proliferation of HPASMCs and the expression of cyclin D1 mRNA and protein were detected.Data were compared with one-way ANOVA(for multiple-group comparison)and independent t-test(for two-group comparison)followed by the least significant difference(LSD)test with the computer software SPSS 17.0.It was found that 0.5%and 1%CSE could promote the proliferation of HPASMCs(P<0.05),and the former was more effective than the latter(P<0.05),while 3%and above CSE had inhibitory effect on HPASMCs(P<0.05).The mRNA and protein expression levels of TRPC1 and cyclin D1 in 0.5%and 1%CSE groups were significantly higher than those in the control group(P<0.05),while those in 3%CSE group were significantly decreased(P<0.05).Moreover,the proliferation of HPASMCs and the expression of cyclin D1 mRNA and protein in TRPC1-siRNA transfection group were significantly reduced as compared with those in the negative control group(P<0.05).It was concluded that low concentration of CSE can promote the proliferation of HPASMCs,while high concentrations of CSE inhibit HPASMCs proliferation.These findings suggested that CSE induced proliferation of HPASMCs at least in part via TRPC1-mediated cyclin D1 expression. 展开更多
关键词 cigarette smoke extract human pulmonary artery smooth muscle cells transient receptor potential channel 1 cyclin D1
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Effect of Cigarette Smoke Extract on the Proliferation of Human Airway Epithelial Cells and Expression and Activation of FAK
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作者 许丽 张珍祥 徐永健 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2005年第3期265-268,共4页
The effect of cigarette smoke extract (CSE) on the proliferation of human airway epithelial cells and the possible mechanism was studied. After airway epithelial cells were treated with different concentrations of CSE... The effect of cigarette smoke extract (CSE) on the proliferation of human airway epithelial cells and the possible mechanism was studied. After airway epithelial cells were treated with different concentrations of CSE for 24 h, the cell proliferation was measured by MTT and the distribution of different cell cycles by flow cytometry. The FAK expression level was detected by Western blot and the degree of tyrosine phosphorylation by immunoprecipitation. The results showed that CSE could inhibit the proliferation of human airway epithelial cells, arrest the epithelial cells in G1 phase of cell cycle, dramatically decrease the number of epithelial cells in S and G2 phases; Meanwhile CSE could decrease the expression level of FAK and the degree of its tyrosine phosphorylation. The above effects of CSE were concentration-dependent. The expression of FAK and the degree of its phosphorylation was positively correlated to the increased number of epithelial cells in G1 phase, and negatively to the number of epithelial cells in S and G2 phases. It was concluded that the mechanism by which CSE could inhibit the proliferation of human epithelial cells was contributed to the increased expression and activation of FAK. 展开更多
关键词 cigarette smoke extract airway epithelial cell PROLIFERATION FAK
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Effect of Cigarette Smoke on Diabetic Skin and Protection with Topical Administration of Pinus halepensis Extract
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作者 Varvara Zoumpliou Maria Stamatiadi +8 位作者 Clio Vassiliadis Michail Rallis Georgios Theodoros Papaioannou Sotirios Liakos Alexandros Angelou Styliani Daskalaki Maria Kyriazi Vasilios Roussis Constantinos Vagias 《American Journal of Plant Sciences》 2014年第26期3964-3973,共10页
Compared to normal, diabetic skin is characterized by great sensitivity. Oxidative stress is directly involved, contributing to accelerated skin aging, xerodermia and poor wound healing. In the last 10 years, cigarett... Compared to normal, diabetic skin is characterized by great sensitivity. Oxidative stress is directly involved, contributing to accelerated skin aging, xerodermia and poor wound healing. In the last 10 years, cigarette smoke (CS) exposure has been associated with several skin and dermatological conditions and is directly related to the oxidative stress affecting the skin. However, limited data exist concerning the effect of CS on diabetic skin. Some of the effects of cigarette smoke exposure on the skin of hairless diabetic mice were hereby studied and the potential skin protection by topical applications of Pinus halepensis bark extract was investigated. Female hairless SKH-2 diabetic mice were exposed for 8 days to tobacco smoke and topical applications were performed twice daily. Biophysical parameters such as transepidermal water loss (TEWL), skin elasticity and erythema were measured. In addition, the oxidative stress was evaluated. The results show that diabetes and CS have a synergistic negative action on skin condition, with the development of xerosis and high ROS levels whilst topical applications of Pinus halepensis bark extract protect efficiently the toxic effect of CS on skin, by decreasing skin dryness, oxidative stress and blood glucose levels. 展开更多
关键词 CIGARETTE smoke SKIN Diabetes Mice PINUS halepensis BARK extract
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Inhibitory Effect of Cigarette Smoke Extract on Experimental Lung Metastasis of Mouse Melanoma by Suppressing Tumor Invasion
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作者 Yuta Takahashi Shizuyo Horiyama +5 位作者 Yoko Kimoto Noriko Yoshikawa Masaru Kunitomo Satomi Kagota Kazumasa Shinozuka Kazuki Nakamura 《Pharmacology & Pharmacy》 2012年第3期316-321,共6页
We investigated the effect of a nicotine-and tar-free cigarette smoke extract (CSE) using an experimental metastasis mouse model which was intravenously injected with B16-BL6 mouse melanoma cells. Three-hour pretreatm... We investigated the effect of a nicotine-and tar-free cigarette smoke extract (CSE) using an experimental metastasis mouse model which was intravenously injected with B16-BL6 mouse melanoma cells. Three-hour pretreatment of cells with various concentrations of CSE (0, 0.1, 0.3, and 1%) dose-dependently reduced the number of lung metastatic nodules 14 days after tumor injection. To elucidate the mechanism of this anti-metastatic effect of CSE, we examined the invasion and migration activities of B16-BL6 cells pretreated with CSE for three hours in vitro. CSE significantly reduced the invasion of cells at 1% and the migration at 0.3% and 1%. Under the same pretreatment conditions, CSE had no effect on the proliferation of cells. These findings suggest that CSE contains some ingredients that suppress hematogenic lung metastasis via inhibition of the invasion and migration activities of mouse melanoma cells. 展开更多
关键词 CIGARETTE smoke extract (CSE) ANTI-METASTASIS B16-BL6 Mouse MELANOMA Cells INVASION Migration
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Cigarette smoke extract-induced 16HBE-derived exosomal miR-186 significantly promoted the proliferation of COPD MRC-5 cell
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作者 Dong-Chuan Xu Chan-Yi He +1 位作者 Qi Lin Yi-Peng Ding 《Journal of Hainan Medical University》 2021年第11期14-18,共5页
Objective:To study the effect of cigarette extract(CSE)on the expression of exosomal miR-186 derived from 16HBE cells and the effects of 16HBE-derived exosomal miR-186 on the proliferation of MRC-5 cells.Methods:To co... Objective:To study the effect of cigarette extract(CSE)on the expression of exosomal miR-186 derived from 16HBE cells and the effects of 16HBE-derived exosomal miR-186 on the proliferation of MRC-5 cells.Methods:To collect the exosomal miR-186 in the supernatant of CSE-treated 16HBE cells for MRC-5 cell culture;the expression of exosomal miR-186 was detected by qPCR;the proliferation of MRC-5 cell was detected by CCK-8;dual-luciferase reporter gene experiment was used to detect the targeted regulation relationship between miR-186 and Bcl2L11;the expression of Bcl2L11 was detected by Western blot.Results:The morphology of 16HBE cells about 100 nm in diameter were observed by TEM;CSE treatment significantly promoted the expression of exosomal miR-186;CSE-induced exosomal miR-186 promoted the proliferation of MRC-5 cells;Bcl2L11 is a target gene of miR-186;miR-186 mimics significantly decreased Bcl2L11 expression.Conclusion:CSE-induced 16HBE-derived exosomal miR-186 promoted the proliferation of MRC-5 cells by targeting Bcl2L11 genes. 展开更多
关键词 Chronic obstructive pulmonary disease Cigarette smoke extract EXOSOMES miR-186
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Hybrid Deep VGG-NET Convolutional Classifier for Video Smoke Detection 被引量:3
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作者 Princy Matlani Manish Shrivastava 《Computer Modeling in Engineering & Sciences》 SCIE EI 2019年第6期427-458,共32页
Real-time wild smoke detection utilizing machine based identification method is not produced proper accuracy,and it is not suitable for accurate prediction.However,various video smoke detection approaches involve mini... Real-time wild smoke detection utilizing machine based identification method is not produced proper accuracy,and it is not suitable for accurate prediction.However,various video smoke detection approaches involve minimum lighting,and it is required for the cameras to identify the existence of smoke particles in a scene.To overcome such challenges,our proposed work introduces a novel concept like deep VGG-Net Convolutional Neural Network(CNN)for the classification of smoke particles.This Deep Feature Synthesis algorithm automatically generated the characteristics for relational datasets.Also hybrid ABC optimization rectifies the problem related to the slow convergence since complexity is reduced.The proposed real-time algorithm uses some pre-processing for the image enhancement and next to the image enhancement processing;foreground and background regions are separated with Otsu thresholding.Here,to regulate the linear combination of foreground and background components alpha channel is applied to the image components.Here,Farneback optical flow evaluation technique diminishes the false finding rate and finally smoke particles are classified with the VGG-Net CNN classifier.In the end,the investigational outcome shows better statistical stability and performance regarding classification accuracy.The algorithm has better smoke detection performance among various video scenes. 展开更多
关键词 smoke detection foreground extraction optical flow estimation classification FILTERING THRESHOLDING
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HPLC separation of higher fullerenes in the synthetical "graphite smokes" soot
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作者 ZHANG Jun Luann Becker +3 位作者 LIANGHandong LUO Yang XU Wei WANG Hongcui 《Chinese Journal Of Geochemistry》 EI CAS 2012年第3期303-308,共6页
Higher fullerenes(C84,C90,C92,C94 and C96) were successfully isolated from the Soxhlet extract of the synthetical "Graphote Smokes" soot(GS sample) by using a big Cosmosil Buckysep(Phenomenex) column(250 mm ... Higher fullerenes(C84,C90,C92,C94 and C96) were successfully isolated from the Soxhlet extract of the synthetical "Graphote Smokes" soot(GS sample) by using a big Cosmosil Buckysep(Phenomenex) column(250 mm × 10 mm) with a large injection.The fractions isolated have been determined by high performance liquid chro-matography(HPLC) and laser desorption mass spectrometry(LDMS).It is found that there are different fullerenes molecules in different fractions with retention time.The result indicates that fullerenes do exist in GS samples.Also,it excludes the suspicion to some extent that fullerene molecules might be generated by the laser desorption process in the LDMS.In addition,it also provides the experimental basis for the study of natural higher fullerenes and might be helpful to figure out the question if higher fullerenes do exist in the natural samples. 展开更多
关键词 富勒烯分子 HPLC 分离 高效液相色谱仪 煤烟 吸烟 石墨 大容量注射剂
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重组CC16蛋白质抑制香烟烟雾提取物诱导人支气管上皮细胞衰老的初步研究
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作者 李婷 杨晓雪 +3 位作者 高睿 栗馨洋 王海龙 庞敏 《陆军军医大学学报》 CAS CSCD 北大核心 2024年第12期1410-1416,共7页
目的探讨重组人CC16蛋白质(rhCC16)对香烟烟雾提取物(cigarette smoke extract,CSE)诱导的人支气管上皮细胞(human bronchial epithelial cells,HBECs)衰老的抑制作用及机制。方法CCK-8法检测CSE(0%、1%、2.5%、5%、7.5%和10%)及rhCC16(... 目的探讨重组人CC16蛋白质(rhCC16)对香烟烟雾提取物(cigarette smoke extract,CSE)诱导的人支气管上皮细胞(human bronchial epithelial cells,HBECs)衰老的抑制作用及机制。方法CCK-8法检测CSE(0%、1%、2.5%、5%、7.5%和10%)及rhCC16(0、10、100、250和500 ng/mL)对HBECs细胞活力的影响;β-半乳糖苷酶染色试剂盒检测对照组、CSE组以及CSE+rhCC16组细胞β-半乳糖苷酶活性;实时荧光定量PCR(qPCR)检测各组细胞P16、P21 mRNA表达水平;Western blot检测各组细胞P16、P21、p-P53、P38、p-P38、ERK1/2、p-ERK1/2蛋白表达水平。结果与对照组相比,5%CSE刺激细胞72 h对HBECs细胞活力无显著影响;500 ng/mL及以下浓度rhCC16对HBECs细胞活力无显著影响;与对照组相比,5%CSE刺激HBECs细胞72 h致β-半乳糖苷酶染色阳性细胞率明显升高(P<0.05),且P16和P21蛋白和mRNA表达水平明显升高(P<0.05),p-P53、p-P38与p-ERK1/2蛋白表达量显著增加(P<0.05);与CSE组相比,250 ng/mL rhCC16干预下,HBECs细胞β-半乳糖苷酶染色阳性细胞率显著下降(P<0.05),P16和P21蛋白和mRNA表达水平显著下降(P<0.05),p-P53、p-p38和p-ERK1/2的表达量降低(P<0.05)。结论rhCC16蛋白质抑制香烟烟雾诱导的HBECs衰老,抑制P38 MAPK和ERK1/2信号通路可能是其作用机制。 展开更多
关键词 细胞衰老 香烟烟雾提取物 CC16蛋白质 P38 MAPK/ERK1/2
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隧道单向大间距点式排烟设计方法研究
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作者 姜学鹏 张子迪 陈玉远 《安全与环境学报》 CAS CSCD 北大核心 2024年第6期2220-2229,共10页
排烟口间距≤60 m、开启数量不少于3处的重点排烟模式已在隧道中广泛应用。针对其存在因间距小而排烟口数量多、漏风量大问题,提出了火源上游侧纵向通风+下游侧精准开启1处排烟口(含多个排烟阀)的新型单向大间距点式排烟模式。考虑参数... 排烟口间距≤60 m、开启数量不少于3处的重点排烟模式已在隧道中广泛应用。针对其存在因间距小而排烟口数量多、漏风量大问题,提出了火源上游侧纵向通风+下游侧精准开启1处排烟口(含多个排烟阀)的新型单向大间距点式排烟模式。考虑参数间相互关联性,基于特殊消防设计思路,构建了单向大间距点式排烟模式有效性评估模型,并提出了优化设计方法。依托实际隧道工程,通过数值模拟对新型点式排烟模式进行了研究,探讨了不同排烟阀个数、纵向风速、排烟口间距及坡度对烟控效果的影响,结果表明:考虑隧道火灾最不利情况火源功率50 MW下,推荐纵向风速3.67 m/s、1处排烟口(含12个排烟阀)、排烟口间距300 m的通风排烟方案。并采用缩尺寸模型试验,验证了新型点式排烟设计的合理性。 展开更多
关键词 安全工程 隧道火灾 单向大间距 点式排烟 设计方法 评估模型
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隧道火灾重点排烟系统烟气控制效果及排烟效率研究 被引量:2
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作者 申哲玮 郭庆华 李兴莉 《消防科学与技术》 CAS 北大核心 2024年第2期189-196,共8页
采用数值计算方法,考虑热释放速率、排烟量、排烟口间距、排烟口面积、尺寸比和隧道高度,研究隧道火灾重点排烟系统下烟气蔓延距离、温度分布、排烟口风速和排烟效率。结果表明:火灾烟气蔓延距离随排烟量的增大而缩短,隧道顶棚温度下降... 采用数值计算方法,考虑热释放速率、排烟量、排烟口间距、排烟口面积、尺寸比和隧道高度,研究隧道火灾重点排烟系统下烟气蔓延距离、温度分布、排烟口风速和排烟效率。结果表明:火灾烟气蔓延距离随排烟量的增大而缩短,隧道顶棚温度下降;在排烟量不变的条件下,更大的排烟口宽长比能够缩短烟气蔓延距离;而排烟口间距的变化对排烟效率影响不明显。基于研究结果,给出了5 MW和20 MW火源功率下的重点排烟系统排烟量和排烟口布置建议值。 展开更多
关键词 隧道火灾 重点排烟 排烟量 排烟口尺寸 排烟效率
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高大宴会厅防排烟系统改造效果研究 被引量:1
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作者 邹璐 韩如冰 《制冷与空调(四川)》 2024年第4期525-530,共6页
高大空间防排烟系统的安全性和可靠性非常重要。宁波市某宾馆防排烟系统需要改造,其中宴会厅为高大空间。针对宴会厅的防排烟系统改造设计方案进行数值模拟研究,首先根据现行规范计算排烟量,设计排烟口,计算排烟量比原设计排烟量减少41%... 高大空间防排烟系统的安全性和可靠性非常重要。宁波市某宾馆防排烟系统需要改造,其中宴会厅为高大空间。针对宴会厅的防排烟系统改造设计方案进行数值模拟研究,首先根据现行规范计算排烟量,设计排烟口,计算排烟量比原设计排烟量减少41%,排烟风口减少1个;然后采用Pyrosim软件模拟改造前后烟气扩散情况,监测宴会厅内能见度、温度和CO浓度。模拟结果表明:安全疏散时间内,改造后的烟气一直保持在距地面5m以上,人眼特征高度处的温度最高降低3℃,建筑顶棚温度最高降低20℃,最小清晰高度处的CO浓度最大可降低0.6PPM。改造后的防排烟系统方案更安全可靠。 展开更多
关键词 高大空间 防排烟系统 数值模拟 烟气扩散 改造效果
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