This study aims to evaluate the subacute toxic effects of oil under different treatments on marine organism by simulating natural contaminative processes. In this study, 120#(RMD15) fuel oil was selected as the pollut...This study aims to evaluate the subacute toxic effects of oil under different treatments on marine organism by simulating natural contaminative processes. In this study, 120#(RMD15) fuel oil was selected as the pollutant and marine medaka(Oryzias melastigma) embryos as the experimental organism. The developmental toxicity of different volume concentrations(0.05%, 0.2%, 1% and 5%) of water-accommodated fractions, biologicallyenhanced water-accommodated fractions, and chemically-enhanced water-accommodated fractions on the embryos in different exposure time(8, 15 and 22 d) were compared and the content of relevant polycyclic aromatic hydrocarbons(PAHs) was studied(in dispersion and in vivo). The subacute toxic effects were assessed in terms of antioxidant activities of enzymes(superoxide dismutase, catalase and glutathione S-transferase) and the blue sac disease(BSD) indexes.The results showed that the BSD indexes of the treatment groups were significantly higher than the respective control groups and showed positive correlations with both concentration and exposure time. The experiments with three antioxidant enzymes indicated that enzymatic activities of the embryos changed dramatically under the oxidation stress of petroleum hydrocarbons, especially after adding the dispersants. With the increase of petroleum hydrocarbon concentration and exposure time, the three enzymes showed different degrees of induction and inhibition effects.展开更多
Perfluorobutanesulfonate(PFBS)is an emerging pollutant capable of potently disrupting the sex and thyroid endocrine systems of teleosts.However,the hypoxic and temporal variation in PFBS endocrine disrupting toxicity ...Perfluorobutanesulfonate(PFBS)is an emerging pollutant capable of potently disrupting the sex and thyroid endocrine systems of teleosts.However,the hypoxic and temporal variation in PFBS endocrine disrupting toxicity remain largely unknown.In the present study,adult marine medaka were exposed to environmentally realistic concentrations of PFBS(0 and 10μg/L)under normoxia or hypoxia conditions for 7 days,aiming to explore the interactive behavior between PFBS and hypoxia.In addition,PFBS singular exposure was extended till 21days under normoxia to elucidate the time-course progression in PFBS toxicity.The results showed that hypoxia inhibited the growth and caused the suspension of egg spawn regardless of PFBS exposure.With regard to the sex endocrine system,7-day PFBS exposure led to an acute stimulation of transcriptional profiles in females,which,subsequently,recovered after the 21-day exposure.The potency of hypoxia to disturb the sex hormones was much stronger than PFBS.A remarkable increase in estradiol concentration was noted in medaka blood after hypoxia exposure.Changes in sex endocrinology of coexposed fish were largely determined by hypoxia,which drove the formation of an estrogenic environment.PFBS further enhanced the endocrine disrupting effects of hypoxia.However,the hepatic synthesis of vitellogenin and choriogenin,two commonly used sensitive biomarkers of estrogenic activity,failed to initiate in response to the estrogen stimulus.Compared to sex endocrine system,disturbances in thyroidal axis by PFBS or hypoxia were relatively mild.Overall,the present findings will advance our toxicological understanding about PFBS pollutant under the interference of hypoxia.展开更多
基金supported by the National Natural Science Foundation of China(No.40632009,20607002)the National Basic Research Program(973)of China(No.2007CB407304).
文摘尽管 1,1-dichloro-2,2-bis (p-chlorophenyl ) 乙烯(p， p -DDE), dichlorodiphenyltrichloroethane (DDT ) 的主要、很坚持的代谢物，不断地在显示出象间性那样的反常性腺开发的野生的鱼被检测，很少对 p 的影响被知道，在在鱼的性腺开发的 p -DDE 暴露。调查 p，在 gonadal 开发的 p -DDE 和基因表达式的效果，男少年(20-d 帖子开口) 日本 med 也称为(Oryzias latipes ) 暴露于 1， 5， 20，和 100 μ g /L p，为二个月的 p -DDE。增加的 hepatosomatic 索引(HSI ) 和减少的 gonadosomatic 索引(GSI ) 在 p 被发现， p -DDE-treated 组。间性在 100 μ g /L p， p -DDE 暴露组，以及 100 ng/L17 α - ethynylestradiol (EE2 ) 被发现组。由量的即时 RT-PCR，它被发现那基因 vitellogenins 的表情(VTG-1， VTG-2 ) ， choriogenins (CHG-H， CHG-L ) ，并且雌激素受体α(嗯 -- 在鱼的肝的α) 由 p 是显著地起来调整的， p -DDE 暴露。VTG-1 和 VTG- 2 为估计 anti-androgenic p 被推荐为比较喜欢的简历标记， p -DDE 因为他们是在基因之中最高起来调整、显示出好剂量反应关系。起来调整嗯 -- 当时，α建议潜在的合作效果将发生 p， p -DDE 与其它一起共存嗯 -- α - 绑定内分泌破坏的化学药品(EDC ) 。
基金The National Natural Science Foundation of China under contract No.41276105/D0608
文摘This study aims to evaluate the subacute toxic effects of oil under different treatments on marine organism by simulating natural contaminative processes. In this study, 120#(RMD15) fuel oil was selected as the pollutant and marine medaka(Oryzias melastigma) embryos as the experimental organism. The developmental toxicity of different volume concentrations(0.05%, 0.2%, 1% and 5%) of water-accommodated fractions, biologicallyenhanced water-accommodated fractions, and chemically-enhanced water-accommodated fractions on the embryos in different exposure time(8, 15 and 22 d) were compared and the content of relevant polycyclic aromatic hydrocarbons(PAHs) was studied(in dispersion and in vivo). The subacute toxic effects were assessed in terms of antioxidant activities of enzymes(superoxide dismutase, catalase and glutathione S-transferase) and the blue sac disease(BSD) indexes.The results showed that the BSD indexes of the treatment groups were significantly higher than the respective control groups and showed positive correlations with both concentration and exposure time. The experiments with three antioxidant enzymes indicated that enzymatic activities of the embryos changed dramatically under the oxidation stress of petroleum hydrocarbons, especially after adding the dispersants. With the increase of petroleum hydrocarbon concentration and exposure time, the three enzymes showed different degrees of induction and inhibition effects.
基金supported by the National Natural Science Foundation of China (Nos.22006159 and 31971236)the Research Grants Council Theme-Based Research Scheme (No.T21-602/16-R)+1 种基金the Natural Science Foundation of Hubei Province,China (No.2021CFA086)the State Key Laboratory of Freshwater Ecology and Biotechnology (No.2022FBZ02)。
文摘Perfluorobutanesulfonate(PFBS)is an emerging pollutant capable of potently disrupting the sex and thyroid endocrine systems of teleosts.However,the hypoxic and temporal variation in PFBS endocrine disrupting toxicity remain largely unknown.In the present study,adult marine medaka were exposed to environmentally realistic concentrations of PFBS(0 and 10μg/L)under normoxia or hypoxia conditions for 7 days,aiming to explore the interactive behavior between PFBS and hypoxia.In addition,PFBS singular exposure was extended till 21days under normoxia to elucidate the time-course progression in PFBS toxicity.The results showed that hypoxia inhibited the growth and caused the suspension of egg spawn regardless of PFBS exposure.With regard to the sex endocrine system,7-day PFBS exposure led to an acute stimulation of transcriptional profiles in females,which,subsequently,recovered after the 21-day exposure.The potency of hypoxia to disturb the sex hormones was much stronger than PFBS.A remarkable increase in estradiol concentration was noted in medaka blood after hypoxia exposure.Changes in sex endocrinology of coexposed fish were largely determined by hypoxia,which drove the formation of an estrogenic environment.PFBS further enhanced the endocrine disrupting effects of hypoxia.However,the hepatic synthesis of vitellogenin and choriogenin,two commonly used sensitive biomarkers of estrogenic activity,failed to initiate in response to the estrogen stimulus.Compared to sex endocrine system,disturbances in thyroidal axis by PFBS or hypoxia were relatively mild.Overall,the present findings will advance our toxicological understanding about PFBS pollutant under the interference of hypoxia.
