Zhuang medicine medicated thread moxibustion is a kind of external therapy with ethnic medicine characteristics that spreads in Guangxi Zhuang region.It has a wide range of clinical application and significant curativ...Zhuang medicine medicated thread moxibustion is a kind of external therapy with ethnic medicine characteristics that spreads in Guangxi Zhuang region.It has a wide range of clinical application and significant curative effect,and can be used to treat diseases such as internal medicine,surgery,dermatology,gynecology,pediatrics,and five sense organs.Through reviewing and combing the literature on the Zhuang medicine medicated thread moxibustion in recent years,this paper summarizes and prospects its clinical application,with a view to further explaining the basic principles and application rules of Zhuang medicine medicated thread moxibustion and digging its clinical value.展开更多
Objective:To explore the efficacy and safety of Zhuang medicine medicated thread moxibustion(ZMTM)on psoriasis vulgaris.Methods:A multicenter,randomized,parallel controlled clinical trial was designed.A total of 241 o...Objective:To explore the efficacy and safety of Zhuang medicine medicated thread moxibustion(ZMTM)on psoriasis vulgaris.Methods:A multicenter,randomized,parallel controlled clinical trial was designed.A total of 241 outpatients with psoriasis vulgaris were randomly divided into a control group(120 cases)and a treatment group(121 cases)using a central block randomization from June 2015 to May 2018.The control group was treated with Western medicines alone including pidotimod dispersible tablets,vitamin B compound tablets,and compound cod liver oil-zinc oxide ointment.The treatment group was treated with ZMTM every 2 days combined with Western medicines.The two groups received continuous intervention for 30 days.The primary outcome was Psoriasis Area and Severity Index(PASI),and the secondary outcomes included Itch Rating Scale,Dermatology Quality of Life Index(DLQI),Hamilton Anxiety Rating Scale(HAMA),as well as PASI response rate.Meanwhile,adverse events were evaluated during the whole clinical trial.Follow-up was carried out 30 days after treatment.Results:There were 5 cases of shedding in this trial.In intention-to-treat analysis,236 cases were included and each group contained 118 cases.On the 30th and 60th days,PASI scores of patients in each group were significantly lower than that at baseline(P<0.01)and the PASI score reduction of the treatment group was greater than that of the control group(P<0.01).Itch Rating Scale,DLQI,and HAMA scale were decreased in both groups after treatment,and the treatment group showed a better therapeutic effect(P<0.01).The response rates of PASI 50 and 75 were significantly higher than those in the control group[81.4%(96/118),43.2%(51/118)vs.41.5%(49/118),11.0%(13/118),respectively,P<0.05].During follow-up,the improvements in scores of PASI,Itch Rating Scale,DLQI,and HAMA of the treatment group were significantly greater than those of the control group(P<0.01).The response rates of PASI 50 and 75 in the treatment group were significantly higher than those in the control group,respectively(both P<0.05).No obvious adverse reaction was found in either group.Conclusion:ZMTM combined with Western medicines showed a better therapeutic effect in the treatment of psoriasis vulgaris without obvious adverse reaction.(Trial Registration No.ChiCTR-IOR-16008159)展开更多
OBJECTIVE: To investigate the effect and underlying mechanisms of combined medicated thread moxibustion therapy plus needle picking therapy of Zhuang nationality medicine on antioxidant levels in a rat model of sciati...OBJECTIVE: To investigate the effect and underlying mechanisms of combined medicated thread moxibustion therapy plus needle picking therapy of Zhuang nationality medicine on antioxidant levels in a rat model of sciatica.METHODS: One hundred Wistar rats, of specific pathogen free level, were randomly divided into five groups: normal control group, model group,medicated thread moxibustion group, needle picking group, and combination group. Each group contained 20 rats. In the model, medicated thread moxibustion, needle picking, and combination groups,sciatica models were established through chronicconstriction injury of the sciatic nerve. After the model was established, the rats in the medicated thread moxibustion, needle picking, and combination groups were given the corresponding therapies for 21 days. The control and model groups received no treatment. Reactive oxygen species, superoxide dismutase, malondialdehyde, and total antioxidant capacity changes were determined.Nicotinamide adenine dinucleotide phosphate(NADPH) oxidase subunit NADPH oxidases 4(NOX4) m RNA expression and the morphology of cells were observed to detect apoptosis of gamma-aminobutyric acid ergic(GABAergic) neurons.RESULTS: Compared with control group, reactive oxygen species and malondialdehyde levels rose significantly in the model group(P < 0.01), while superoxide dismutase and total antioxidant capacity levels were lowered(P < 0.05). Compared with the model group, reactive oxygen species and malondialdehyde decreased in the needle picking group(P < 0.05), while superoxide dismutase levels were increased(P < 0.05); reactive oxygen species and malondialdehyde significantly decreased in the combination group(P < 0.01). In addition, the model group had higher NOX4 m RNA expression than that of the control group(P < 0.05), and the combination group had lower expression levels than that of the model group(P < 0.05). Apoptosis of GABAergic neurons was observed in the model group, and was attenuated after combined therapy.CONCLUSION: The medicated thread moxibustion therapy plus needle picking therapy of Zhuang nationality medicine can prevent oxidative damage in the rat model of sciatica via down-regulating NOX4 expression, improving antioxidant capacity, and in-hibiting the oxidative damage pathway of GABAergic neurons.展开更多
目的:运用代谢组学方法研究刺血结合药线治疗大鼠急性痛风性关节炎的机制。方法:Wistar成年雄性大鼠30只,随机分为模型组、西药组、治疗组,每组10只,治疗结束后,收集3组大鼠血清样本,运用气相色谱仪与飞行时间质谱仪的联用(gas chromato...目的:运用代谢组学方法研究刺血结合药线治疗大鼠急性痛风性关节炎的机制。方法:Wistar成年雄性大鼠30只,随机分为模型组、西药组、治疗组,每组10只,治疗结束后,收集3组大鼠血清样本,运用气相色谱仪与飞行时间质谱仪的联用(gas chromatographytime-of-flight mass spectrometry,GC-TOF-MS)代谢组学技术分析大鼠血清代谢色谱,并进行主成分分析法(PCA)和正交偏最小二乘方-判别分析(OPLS-DA)。结果:治疗组大鼠步态较西药组大鼠好,其肿胀度也低;治疗组较模型组的代谢物能抗炎镇痛;治疗组较西药组的代谢物能促进代谢,降尿酸。结论:采用代谢组学的方法,较全面的反应了刺血结合药线治疗急性痛风性关节炎大鼠的代谢状态,其对血清代谢物影响明显。展开更多
目的:观察针刺联合壮医药线点灸对睡眠剥夺大鼠相关神经递质的影响。方法:80只SPF级Wistar大鼠随机分为对照组、模型组、治疗组、安定组4组,每组20只。模型组、治疗组、安定组通过睡眠剥夺进行失眠模型造模。治疗组在造模基础上给予针...目的:观察针刺联合壮医药线点灸对睡眠剥夺大鼠相关神经递质的影响。方法:80只SPF级Wistar大鼠随机分为对照组、模型组、治疗组、安定组4组,每组20只。模型组、治疗组、安定组通过睡眠剥夺进行失眠模型造模。治疗组在造模基础上给予针刺和壮医药线点灸治疗,每日1次,连续治疗14天。安定组按照3 mg·kg-1体质量给予安定灌胃,对照组则给予等体积生理盐水灌胃,均是每日1次,连续灌胃14天。检测增食素(Orexin)、去甲肾上腺素(Norepinephrine,NE)、5-羟色胺(5-Hydroxytryptamine,5-HT)、促肾上腺皮质激素释放激素(Corticotropin-Releasing Hormone,CRH)、促肾上腺皮质激素(Adrenocorticotropic Hormone,ACTH)水平,观察Orexin m RNA、c-Fos表达。结果:与对照组比较,模型组大鼠Orexin、ACTH、5-HT、NE、CRH水平升高或明显升高,差异均有统计学意义(P<0.05或P<0.01);模型组大鼠Orexin m RNA、c-Fos表达升高或明显升高,差异均有统计学意义(P<0.05或P<0.01)。与模型组比较,治疗组大鼠Orexin、ACTH、5-HT、NE、CRH水平下降或明显下降,差异也均有统计学意义(P<0.05或P<0.01);治疗组大鼠Orexin m RNA、c-Fos表达水平下降或明显下降,差异也均有统计学意义(P<0.05或P<0.01)。结论:针刺联合壮医药线点灸通过影响Orexin,进而调控下丘脑-垂体-肾上腺轴(Hypothalamic Pituitary Adrenal axis,HPA axis),降低应激反应性,促进觉醒向睡眠的转变可能是其治疗失眠的机制之一。展开更多
基金"Zhuang medicine",a key discipline of traditional Chinese medicine in Guangxi(No.GZXK-Z-20-60),the project to improve the basic scientific research ability of young and middle-aged teachers in Guangxi universities(No.2020KY07023),and the scientific research project of Guangxi Zhuang Autonomous Region Administration of Traditional Chinese Medicine(No.GXZYZ20210456).
文摘Zhuang medicine medicated thread moxibustion is a kind of external therapy with ethnic medicine characteristics that spreads in Guangxi Zhuang region.It has a wide range of clinical application and significant curative effect,and can be used to treat diseases such as internal medicine,surgery,dermatology,gynecology,pediatrics,and five sense organs.Through reviewing and combing the literature on the Zhuang medicine medicated thread moxibustion in recent years,this paper summarizes and prospects its clinical application,with a view to further explaining the basic principles and application rules of Zhuang medicine medicated thread moxibustion and digging its clinical value.
基金Supported by the Scientific Research Special Fund of Traditional Chinese Medicine Industry(No.201507006)。
文摘Objective:To explore the efficacy and safety of Zhuang medicine medicated thread moxibustion(ZMTM)on psoriasis vulgaris.Methods:A multicenter,randomized,parallel controlled clinical trial was designed.A total of 241 outpatients with psoriasis vulgaris were randomly divided into a control group(120 cases)and a treatment group(121 cases)using a central block randomization from June 2015 to May 2018.The control group was treated with Western medicines alone including pidotimod dispersible tablets,vitamin B compound tablets,and compound cod liver oil-zinc oxide ointment.The treatment group was treated with ZMTM every 2 days combined with Western medicines.The two groups received continuous intervention for 30 days.The primary outcome was Psoriasis Area and Severity Index(PASI),and the secondary outcomes included Itch Rating Scale,Dermatology Quality of Life Index(DLQI),Hamilton Anxiety Rating Scale(HAMA),as well as PASI response rate.Meanwhile,adverse events were evaluated during the whole clinical trial.Follow-up was carried out 30 days after treatment.Results:There were 5 cases of shedding in this trial.In intention-to-treat analysis,236 cases were included and each group contained 118 cases.On the 30th and 60th days,PASI scores of patients in each group were significantly lower than that at baseline(P<0.01)and the PASI score reduction of the treatment group was greater than that of the control group(P<0.01).Itch Rating Scale,DLQI,and HAMA scale were decreased in both groups after treatment,and the treatment group showed a better therapeutic effect(P<0.01).The response rates of PASI 50 and 75 were significantly higher than those in the control group[81.4%(96/118),43.2%(51/118)vs.41.5%(49/118),11.0%(13/118),respectively,P<0.05].During follow-up,the improvements in scores of PASI,Itch Rating Scale,DLQI,and HAMA of the treatment group were significantly greater than those of the control group(P<0.01).The response rates of PASI 50 and 75 in the treatment group were significantly higher than those in the control group,respectively(both P<0.05).No obvious adverse reaction was found in either group.Conclusion:ZMTM combined with Western medicines showed a better therapeutic effect in the treatment of psoriasis vulgaris without obvious adverse reaction.(Trial Registration No.ChiCTR-IOR-16008159)
基金Supported by the National Natural Science Foundation of China(Research on Mechanism of Insomnia Treatment Administrated by Acupuncture Combined With Medicated Thread Moxibustion of Zhuang Nationality Medicine,No.81360579)
文摘OBJECTIVE: To investigate the effect and underlying mechanisms of combined medicated thread moxibustion therapy plus needle picking therapy of Zhuang nationality medicine on antioxidant levels in a rat model of sciatica.METHODS: One hundred Wistar rats, of specific pathogen free level, were randomly divided into five groups: normal control group, model group,medicated thread moxibustion group, needle picking group, and combination group. Each group contained 20 rats. In the model, medicated thread moxibustion, needle picking, and combination groups,sciatica models were established through chronicconstriction injury of the sciatic nerve. After the model was established, the rats in the medicated thread moxibustion, needle picking, and combination groups were given the corresponding therapies for 21 days. The control and model groups received no treatment. Reactive oxygen species, superoxide dismutase, malondialdehyde, and total antioxidant capacity changes were determined.Nicotinamide adenine dinucleotide phosphate(NADPH) oxidase subunit NADPH oxidases 4(NOX4) m RNA expression and the morphology of cells were observed to detect apoptosis of gamma-aminobutyric acid ergic(GABAergic) neurons.RESULTS: Compared with control group, reactive oxygen species and malondialdehyde levels rose significantly in the model group(P < 0.01), while superoxide dismutase and total antioxidant capacity levels were lowered(P < 0.05). Compared with the model group, reactive oxygen species and malondialdehyde decreased in the needle picking group(P < 0.05), while superoxide dismutase levels were increased(P < 0.05); reactive oxygen species and malondialdehyde significantly decreased in the combination group(P < 0.01). In addition, the model group had higher NOX4 m RNA expression than that of the control group(P < 0.05), and the combination group had lower expression levels than that of the model group(P < 0.05). Apoptosis of GABAergic neurons was observed in the model group, and was attenuated after combined therapy.CONCLUSION: The medicated thread moxibustion therapy plus needle picking therapy of Zhuang nationality medicine can prevent oxidative damage in the rat model of sciatica via down-regulating NOX4 expression, improving antioxidant capacity, and in-hibiting the oxidative damage pathway of GABAergic neurons.
文摘目的:运用代谢组学方法研究刺血结合药线治疗大鼠急性痛风性关节炎的机制。方法:Wistar成年雄性大鼠30只,随机分为模型组、西药组、治疗组,每组10只,治疗结束后,收集3组大鼠血清样本,运用气相色谱仪与飞行时间质谱仪的联用(gas chromatographytime-of-flight mass spectrometry,GC-TOF-MS)代谢组学技术分析大鼠血清代谢色谱,并进行主成分分析法(PCA)和正交偏最小二乘方-判别分析(OPLS-DA)。结果:治疗组大鼠步态较西药组大鼠好,其肿胀度也低;治疗组较模型组的代谢物能抗炎镇痛;治疗组较西药组的代谢物能促进代谢,降尿酸。结论:采用代谢组学的方法,较全面的反应了刺血结合药线治疗急性痛风性关节炎大鼠的代谢状态,其对血清代谢物影响明显。
文摘目的:观察针刺联合壮医药线点灸对睡眠剥夺大鼠相关神经递质的影响。方法:80只SPF级Wistar大鼠随机分为对照组、模型组、治疗组、安定组4组,每组20只。模型组、治疗组、安定组通过睡眠剥夺进行失眠模型造模。治疗组在造模基础上给予针刺和壮医药线点灸治疗,每日1次,连续治疗14天。安定组按照3 mg·kg-1体质量给予安定灌胃,对照组则给予等体积生理盐水灌胃,均是每日1次,连续灌胃14天。检测增食素(Orexin)、去甲肾上腺素(Norepinephrine,NE)、5-羟色胺(5-Hydroxytryptamine,5-HT)、促肾上腺皮质激素释放激素(Corticotropin-Releasing Hormone,CRH)、促肾上腺皮质激素(Adrenocorticotropic Hormone,ACTH)水平,观察Orexin m RNA、c-Fos表达。结果:与对照组比较,模型组大鼠Orexin、ACTH、5-HT、NE、CRH水平升高或明显升高,差异均有统计学意义(P<0.05或P<0.01);模型组大鼠Orexin m RNA、c-Fos表达升高或明显升高,差异均有统计学意义(P<0.05或P<0.01)。与模型组比较,治疗组大鼠Orexin、ACTH、5-HT、NE、CRH水平下降或明显下降,差异也均有统计学意义(P<0.05或P<0.01);治疗组大鼠Orexin m RNA、c-Fos表达水平下降或明显下降,差异也均有统计学意义(P<0.05或P<0.01)。结论:针刺联合壮医药线点灸通过影响Orexin,进而调控下丘脑-垂体-肾上腺轴(Hypothalamic Pituitary Adrenal axis,HPA axis),降低应激反应性,促进觉醒向睡眠的转变可能是其治疗失眠的机制之一。
